Zeiss Axioplan 2 Manual
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|jaguar71||7:15pm on Thursday, March 11th, 2010|
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Zebrafish composite image courtesy of Ayca Cinaroglu, Kirsten Sadler Lab.
This is an upright epi-fluorescence microscope controlled by Zeiss AxioVision software. This microscope has a Zeiss AxioCam MRm camera for capturing fluorescent dyes and a Q-imaging MP3.3 RTV color camera for capturing full color brightfield images. This microscope also has an ApoTome unit that uses structured illumination to perform real-time deconvolution of tissues and cells. Furthermore, it can perform transmitted light imaging techniques such as darkfield, polarized light and DIC.
Fluorescence Filters Fluorophore
DAPI FITC Texas Red Cy5 CFP YFP Spectrum Orange*
D350/50x BP450-490 D560/40x BP640/30 D436/20x HQ500/20x HQ548/20x
400DCLP FT510 595DCLP FT660 455DCLP Q515LP Q565LP
D460/50m BP515-565 D630/60m BP690/50 D480/40m HQ535/30m HQ580/14m
Objectives Magnification Immersion
2.5x* 5x* 10x 20x 40x 40x 63x 100x air air air air air oil oil oil
0.075 0.15 0.3 0.75 0.75 1.3 1.4 1.3
*available upon request
Human endothelium cells.
Quadruple fluorescence: DAPI, Alexa 350, Alexa 488, Phalloidin-Alexa 594. Plan-Neofluar 63/1.25 oil. J. Zbaeren, Insel Hospital, Bern
The decisive gain in contrast: FITC fluorescence micrograph without Light Trap (1), with Light Trap (2)
Multichannel fluorescence is playing an increasingly important role in research, whether used alone or in combination with other optical methods. There is a current trend, for example, from single channel FISH (fluorescence in-situ hybridization) to multicolor FISH, where combinations of up to seven fluorochromes yield 24 colors for automatic chromosome analysis. In many research labs, different mutants of fluorescent proteins are combined with standard fluorescent dyes. So it is becoming more and more important to have six or more positions on the filter turret. With Axioplan 2 imaging, you have eight.
With 8 positions in the lead : Filters and filter turret
Eight filter places, easily accessible in a compact turret without compromising the full 25 mm field of view: This is top performance combined with unequalled flexibility. You can insert a wide range of dichroic mirror-emission filter combinations, Optovar modules and a DIC analyzer for convenient change. If you install the optional external filter changer, you can use up to eight excitation filters as well.
With the Push&Click mechanism, filter cubes are changed within seconds. Fast access to the right filter set: the filter wheel with 8 positions for Push&Click filter cubes
Number One In Performance And Flexibility
Fast changeover : High-resolution four-color banding of human chromosomes. Dr. I. Chudoba, Institute of Human Genetics, Jena, and MetaSystems, Altlussheim Push&Click
With so many options to choose from, flexibility is the key. The new, cube-mounted filter sets can be quickly removed or added to the Axioplan 2 imaging filter turret. No more screws, no problems with alignment: The practical Push&Click mechanism ensures the filters are correctly positioned and held
Metaphase. Left to right:
Inverted and band-enhanced DAPI counterstaining; RGB presentation; classified pseudocolor presentation
securely in place.
Chart of chromosome karyotypes. Comparison of DAPI counterstaining and classified chromosomes. Dr. Khler, Applied Spectral Imaging
Research assignments pose formidable challenges to both people and equipment. Axioplan 2 imaging brilliantly satisfies the demands placed on a microscopy workplace. It is the researchers ideal partner when it comes to getting the best results.
Turn minimum signals into maximum results : Zeiss optics
Suppose you want to observe signals at a cell membrane or flows inside a cell, or investigate the presence of GFP in, or GFP transport between, specific cell compartments. No matter whether the emission signal comes from a fluo15 C. elegans. GFP-labeled neurons. H. Hutter, MPI Heidelberg Development stadia of C. elegans. DIC. Prof. Schnabel, Braunschweig University of Technology
Differentiation by better contrast : DIC with a memory
For studies in developmental biology using DIC microscopy, the maximum in resolution and contrast is only just good enough. To achieve this, all parts of the instrument must be perfectly tuned to each other as is the case with Axioplan 2 imaging. For every objective, there is a specially matched DIC slider, including one with Snarmont contrasting. Once the DIC slider has been set, the objective will keep the setting in mind. Axioplan 2 imaging comes with an extensive range of ICS objectives of renowned, continuously optimized Zeiss quality, with superb contrast and resolution. One of them will be ideally suited to your current application, whatever it may be. rescence-labeled antibody, an iondepicting dye or another specific marker, it is too faint to be detectable with optics of any but the highest performance. Objectives from Carl Zeiss feature highest NA (limited only by the laws of physics), ideal chromatic correction and image flattening, and the very best transmission properties for visible light, UV and IR imaging. In short: maximum performance for every application.
Focus On Research
Perfect specimen protection : The high-speed shutter
Correct excitation : AttoArc 2
With AttoArc 2 you can control the The microscopy of live objects is particularly demanding. In order to get the best images, specimens must be treated with the least possible damage. The motorized high-speed shutter of Axioplan 2 imaging provides a superb advantage in fluorescence work. With shutter speeds down to a few microseconds, it is the perfect solution for GFP images of living systems. Simply leave the light source on and control exposure time with the shutter. This reduces bleaching and phototoxicity of the specimen.
Fast but gentle : The SoftStop function
intensity of the excitation light via the brightness of the lamp source. As a result, you automatically get signals of the intensity needed for documentation. In computer-controlled multichannel fluorescence work, the light intensity will be automatically adjusted from channel to channel. Whether with manual or digital control, AttoArc 2 will always provide signals of optimum strength. And this means perfect results.
With the new SoftStop function, you can change reflector modules and objectives within fractions of a second. This will save valuable time, especially in automated processes. In addition, a special algorithm prevents switching oscillations otherwise inevitable at such speeds a feature that is easy on your microscope and your specimens.
Electronic dimmer for high-pressure lamps: AttoArc 2
Axioplan 2 imaging ICS Optics
Infinity optics were designed in the early 1930s on the basis of August Khlers concepts and calculations. But it took the invention of ICS (Infinity Color-corrected System) in 1986 to make this type of optics the core of a complete range of instruments. ICS optics aim at achieving the best possible performance with the least possible number of optical elements. As every optical element contributes to limiting the systems light transmission, minimizing the number of optical components means maximizing the overall optical performance. ICS optics and SI (System Integration) stand design have brought about visible improvements in optical performance with regard to image contrast, brightness and detail resolution. Today, after more than a decade, the ICS concept is still unsurpassed. With continued upgrading of the imaging elements and advanced manufacturing processes, ICS optics have become indispensable for providing the high apertures and long working distances needed for the applications of today and tomorrow.
Limited Only By The Laws Of Physics
1) Mycelia. Grocott silvering. Prof. Hfler, Munich University of Technology 2) Salivary gland of a drosophila. GFP labeling 3) Cell nuclei and mitosis. Triple fluorescence. Dr. Beensen, Friedrich Schiller University, Jena 4) Embryo of C. elegans. DIC. Prof. Schnabel, Braunschweig University of Technology
If flexibility and a wide range of techniques are required, universal semi-apochromatic Plan-Neofluar objectives are the answer. With transmission from the near UV, semi-apochromatic correction, excellent image flattening, optimum working distances, low strain and high numerical apertures, they are ideal for brightfield, darkfield, phase contrast, DIC, polarization and fluorescence. High contrast makes them perfect for capturing sharp, clear images for post processing and subsequent analysis.
The Fluar range is designed for maximum transmission and photon collection. Constructed from special optical glass, these objectives feature high numerical apertures and high contrast, ensuring the best light transmission throughout the visible spectral range down to the near UV. Fluar objectives are your best choice if you want to capture even the faintest fluorescence signal.
For the examination of living organisms and immersion preparations, you need high-performance objectives capable of optically compensating differing refractive indices and volumes of the media involved. These requirements are best met by the special family of MultiImmersion Plan Neofluar objectives or the apochromatically corrected C-Apo family for the most demanding applications. Both will always give you brilliant images.
Thanks to the combination of best color correction with highest numerical apertures, Plan-Apochromat objectives provide the ultimate in resolution, image definition, and rendition of subtlest color differences. With their large apertures, they deliver outstanding brightfield and DIC images and perform brilliantly in fluorescence microscopy.
Axioplan 2 imaging The Digital Imaging Platform
tor, this allows you to expand your system with video cameras, digital compact cameras or other advanced imagers meaning that your system is one hundred percent future-safe.
Digital micrography : Image quality with AxioCam perfection
AxioCam + Axioplan 2 imaging the ideal combination of high-end camera and microscope provide you with a digital imaging workstation unparalleled in perfection and convenience. No films, no time lost with processing the images are available immediately on a PC screen, perfectly resolved with up to 3900 x 3090 non-interpolated pixels per color channel. The programmable resolution of AxioCam not only ensures perfect documentation but also allows you to digitize the maximum available optical resolution without any losses. This produces brilliant digital image files with the resolution of traditional transparencies ideal for printed publications and your notes, workbooks or records. Of course, they can be incorporated into your web site or sent by email. AxioCam can be used with a wide range of tubes and adapters for micrography. Together with a useful device called the port replicaFor top-quality digital images, whether in color or black&white, AxioCam is the optimum imager for Axioplan 2 imaging. A camera designed by Carl Zeiss to meet the requirements of microscopy one hundred percent. Perfect image quality is ensured by a sophisticated electronic system composed of first-grade devices, including a 14bit analog-to-digital converter. The camera is connected via glass fiber cable so as to be isolated from ambient interferences. Cooled by a Peltier element, the CCD image sensor is kept free from disturbing effects, so that even long exposures needed for very faint fluorescences produce images of excellent quality. AxioCam is the perfect digital choice for brightfield and DIC micrography. It is amazing how well it performs with singleand multichannel fluorescence; in the black&white configuration, it satisfies even the most exacting demands.
Overall view Selective enlargement
Dual capability with many benefits : The multi-imaging tube
Added flexibility: The dual video adapter
In addition to the binocular or Want to use a video camera and a digital camera in parallel? Split up fluorescence signals according to wavelength and document the image at the same time? The new motorized multi-imaging tube, with ports for two cameras, makes it possible. Equipped with a software control interface, with XY camera positioning to ensure perfect image overlay and with a neutral beam splitter mirror in the Push&Click module, which you can replace with a dichroic mirror, the multi-imaging tube opens up any documentation road you want to go with full automation. multi-imaging tube, the dual video adapter provides another optical port. For wavelength selection, you can easily replace the neutral beam splitter with a dichroic mirror, using the Push&Click mechanism just as with the multi-imaging tube. That means that you can assemble the exact system you need for your specific application.
Cat's cerebellum. Silver staining. J. Zbaeren, Insel Hospital, Bern Top: Axioplan 2 imaging with motorized multiimaging tube and dual video adapter Bottom: Axioplan 2 imaging with motorized multi-imaging tube
Dual TV tube
Axioplan 2 imaging Motorization
Whether manually controlled or motorized, Axioplan 2 imaging lets you decide precisely which configuration suits your routine or research tasks best.
Simple and clever : SmartContrast
Precisely in focus : The third dimension Revolving nosepiece
Maximum resolution governs the third dimension as well: Axioplan 2 imaging combines minute, discrete focusing steps with motorized speed, satisfying the most exacting requirements of 3D imaging. Manual focusing is supported by programmable step sizes and separate coarse and fine focusing knobs a first for a motorized microscope. Precision and repeatability at their finest!
Automated convenience : Motorized scanning stages Filter wheels and field diaphragm Condensers Z con
Precise images require precise microscope settings. Every time you change an objective or a contrasting technique, you need to readjust the light source, brightness, filters and diaphragms. Axioplan 2 imaging saves you much of the effort and time involved: The new SmartContrast feature and the motorized DIC condenser allow you to save your favored settings and automatically retrieve them time and again for every objective and every contrast technique. For example, change from 40 x DIC to 40 x FITC at the touch of a single button!
Motorized scanning stages open up almost unlimited possibilities: Let Axioplan 2 imaging scan your specimens overnight; detect, select, locate and group predefined patterns with the intelligent image processing software; effect chromosome karyotyping; produce a high-resolution scan of your specimen, control the microscope remotely, or automatically track the growth of live cells beyond the margin of the visual field. Microscopy unlimited in time and space!
The graphic operator interface of AxioVision Control
High-speed shutter, integrated
Perfection The Automatic Way
8-place filter wheel
Personalized settings : The function keys
To facilitate setting, you, as well as
The motorized components of Axioplan 2 imaging Mot
any other user, can store up to twenty individual, frequently used settings in separate user menus and activate them when needed. Select the field diaphragm setting, the phase annulus, the objective, and the intensity of illumination, and save the information behind a screen button or with even faster access behind a key of your keyboard. Simply strike this key to
Supports your success : Repeatability
Flexible choice : Manual or motorized functions
reproduce the settings in next to no time. In micrography, with every shot you can save the exact settings of all motorized and encoded microscope mechanisms in a database. Reproducibility at a keystroke even in documentation!
The brilliance of images is one of the two decisive quality criteria in microscopy. Reliable repeatability is the other. With its coded and motorized stands and components, Axioplan 2 imaging not only ensures exact documentation of your experiments but also allows all settings made to be exactly reproduced. AxioVisionControl, a basic software package option available with the motorized Axioplan 2 imaging, allows you to control all microscope functions and inquire all settings. Flexible, easy to operate and absolutely user-friendly, AxioVisionControl is also ideal in a multi-user environment.
It is up to you to decide which functions of your microscope should be motorized or coded. Whatever you decide or set is saved by the software whether you make manual settings on a motorized component or select a function on the graphic user interface and see the microscope home in on the selected position. Here is a concept providing you with full control of everything. You decide whether you operate the motorized functions of Axioplan 2 imaging digitally via PC or by hand as with a manual microscope the flexible system design gives you the choice.
Carl Zeiss Digital Technology
Computer-assisted work in microscopy has become a matter of course. In fact, many applications are inconceivable without digital help. Carl Zeiss has developed a package of solutions tailored to specific applications. The solutions comprise software and hardware modules, including a digital camera. Used in conjunction with Axioplan 2 imaging, the package is among the best tools available to digital microscopy today.
Perfection the digital way: AxioVision
Precise image acquisition easily controlled: This is what AxioVision, the Carl Zeiss imaging software system, does for you. From exposure via processing, annotation and archiving to the report AxioVision reliably controls the camera and the microscope, including the automatic storing of magnifications and calibration of the scaling bar when you change objectives. The graphical user interface is easy to handle and clearly laid out, with the main window split into a working and a document area. Configurable menus allow you to adapt AxioVision optimally to your application. Its functionality can be expanded easily and quickly. Supplementary modules enable added capabilities for automatic image acquisition techniques, thereby providing you with the full performance spectrum of a motorized microscope for example: fully automatic control in taking 3D image stacks with motorized focusing, in multichannel fluorescence work, time-resolved series, or deconvolution.
Division of a CHO cell. Bottom left: Original data. Top right: Result after deconvolution
Intelligence in Microscopy
Open-end system: Zeiss & partners
Software tailored to Axioplan applications
When special software is needed for specific research assignments, your partners are our partners. The open architecture of Axioplan 2 imaging allows for easy linking of software packages from other suppliers, making this microscope the ideal workstation for a great variety of special, software-controlled applications in many fields.
Optimized fluorescence : Carl Zeiss deconvolution software
FISH and M-FISH image analysis Telepathology and telemicroscopy Stereometric microscopy Automated 3D, 4D and 6D imaging Automated assay scanning systems (e.g. ELISPOT) General image acquisition in varied fields
Stray light from above or below the focal plane is a familiar problem in fluorescence microscopy. In extreme cases, it completely outshines the fluorescence-labeled structures. Carl Zeiss has solved the problem for you: An efficient 3D deconvolution software mathematically traces the disturbing stray light back to its origin and thus deconvolutes the distorted structure. As a result, you get a noise-free image of high resolution and quality.
Digital imaging Telemikroskopie CAN/SUB-BUS Shutter-Sync Video
Offering many interfaces for the individual control of all motorized components, the Axioplan 2 imaging Mot integrates easily into any system.
LAN WAN ISDN ATM.
Internal data Image data Remote control data
Laser Scanning Microscopy
Its exceptional capabilities make Axioplan 2 imaging a perfect mate to the high-performance laser scanning systems of Carl Zeiss. Where the ultimate in resolution in four or more dimensions is absolutely vital, LSM 510 and its little brother, LSM 5 PASCAL, are the ideal tools. Designed strictly along confocal principles, they deliver first-grade optical slices of cells and tissues and thus guarantee high-contrast, 3D multiple fluorescence images.
Maximum comfort with minimum effort
The Carl Zeiss LSM devices based on Axioplan 2 imaging are complete, advanced high-tech systems and yet they are very easy and convenient to use. Thanks to their complete motorization, a highcapability image database and the re-use concept, you can set the desired system configuration with a single mouse click and start your experiment no matter whether you use the system for one or several applications. Flexibility combined with reproducibility!
High functionality and freedom of choice
Carl Zeiss LSM devices are unequalled for flexibility with regard to scanning with 360 field rotation, scanning zoom, offset and such standard and special scanning modes as spline scan or real region of interest (rROI) scan. Short beam paths and detectors picked for performance make the system extremely sensitive. In conjunction with the precisely adjustable laser power, this guarantees best results, even with long-time experiments. Moreover, the LSM multitracking technique yields multichannel fluorescence images free from emission crosstalk, which are ideal for colocalization measurements of, for
Your entry into confocal microscopy: LSM 5 PASCAL
example, GFP-labeled proteins.
See More, Identify More
Large or small : The LSM family
If you are looking for a cost-effective entry into confocal microscopy that doesn't compromise on image quality, LSM 5 PASCAL from Carl
1) Drosophila embryo. Selected optical slices and projection of the entire stack of 47 slices 2) Section through the kidney of an adolescent mouse. Triple fluorescence. 3D shadow projection of a digitally extracted renal tubule 3) Purkinje cell. Fluorescence (Lucifer yellow) in a vital brain section with multiphoton excitation at 805 nm, surface-rendered 3D projection from 105 optical slices
Zeiss is the system of choice for both single users or small teams. It is versatile in use, with an emphasis on GFP applications. At the high end of the family, LSM 510 permits the use of lasers from UV to the visible region to the near infrared. A special model, LSM 510 NLO, is prepared for multiphoton imaging and can be used for examining thicker tissue samples and observing live specimens in
The powerful software is constantly upgraded. Installed on a high-end PC ready for use, it offers a host of functions for data acquisition in one to five or more dimensions. In addition, varied image processing functions allow you to master almost all applications in cell, developmental and molecular biology, physiology, genetics and other fields of research no matter whether youre studying fixed or living specimens.
vitro and in vivo for prolonged times. In combination with Axioplan 2 imaging, the LSM family comprises a perfect system focused on your success.
Stage carriers, Stages, Condenser carriers
DIC-Prism I / 0.9 (with polarizer) (000000-1005-002) DIC-Prism II / 0.9 (with polarizer) (000000-1005-003) DIC-Prism III / 0.9 (with polarizer) (000000-1005-004)
Mechanical stage 75 x 50 / 240 R with ceramic coating (000000-1067-325) Specimen holder for mechanical stage 75 x 50, suitable for one-hand operation (000000-1067-330)
DIC-Prism II/1.4 (445375-0000-000) DIC-Prism III/1.4 (445376-0000-000)
Achromatic-aplanatic universal condenser 0.9 H (000000-1035-632) Achromatic-aplanatic universal condenser 0.9 H D Ph (445436-0000-000) Achromatic-aplanatic universal condenser 0.9 H D Ph DIC (445439-0000-000) Polarizer for condensers (000000-1028-973)
DIC-Prism I / 0.9 (000000-1004-901) DIC-Prism II / 0.9 (000000-1004-902) DIC-Prism III / 0.9 (000000-1004-903)
Specimen holder, reflected light (000000-1070-588)
Mechanical stage 75 x 50 R with electric vernier scale (000000-1046-520) Display unit SCD (413507-9001-000)
Universal mounting frame for Petri dishes and specimen holder (000000-1100-843)
Achromatic-aplanatic condenser 1.4 H Ph DIC (445453-0000-000)
Heatable universal mounting frame A-H (000000-1116-055) Tempocontrol 37 (1-channel) (000000-1116-057) Achromatic-aplanatic universal condenser 0.9 H MOT (445437-0000-000) Achromatic-aplanatic universal condenser 0.9 H D Ph MOT (445438-0000-000) Achromatic-aplanatic universal condenser 0.9 H D Ph DIC MOT (445440-0000-000) Polarizer for condensers (000000-1028-973)
Specimen holder D Pol (45363-0000-000)
Mechanical stage 75 x 50 right with height-adjustable drive and friction setting (000000-1063-835) Specimen holder, special (particularly for immersion objectives) (000000-1070-589)
Achromatic condenser 0.8 H DIC (000000-1087-444) DIC-Prism III / 0.8 (000000-1087-445)
Specimen holder A Pol (453564-0000-000) Brightfield insert (445467-0000-000)
Mounting frame for heating and cooling devices (000000-1107-544) Kreuztisch 75 x 50 L mit Ergonomietrieb (000000-1063-836) Mechanical stage 75 x 50 mot with control unit (000000-1025-145)
Front optics 0.9 / dia. 2.9 Pol (445331-0000-000)
Specimen holder (acc. Price list) optional Joystick for 2 axes (000000-1033-996) or Coaxial electronic drive (000000-1034-960)
Achromatic condenser 0.8 H D Ph DIC (445445-9901-000) DIC-Prism III / 0.8 (445485-0000-000)
Rotary stage Pol (453550-0000-000) with object guide Pol (453560-0000-000)
Achromatic-aplanatic system condenser 0.24/dia. 10.7, folded Pol (445325-9901-000)
Darkfield illuminator (445314-9901-000) Ultra condenser 1.2/1.4 (0.75-1.0) (465500-0000-000) Dry darkfield condenser 0.8 / 0.95 (0.6 - 0.75) (465505-0000-000) Condenser carrier with left and right hand controls for vertical adjustment (000000-1101-554)
Stage carrier fixed with condenser carrier Axioplan 2 imaging (000000-1062-273)
Condenser holder Z (445323-0000-000)
Stage carrier, lowerable and detachable (000000-1062-274)
Stand, Tubes, Eyepieces, Filters, Reflectors
Intermediate tubes, Nosepieses
Double video adapter (000000-1058-640) Binocular phototube with height adjustment (452146-0000-000)
Optovar intermediate tube 1.0x/1.25x/1.6x/2.0x/2.5x cod. (452175-0000-000) Double TV-tube mot. with 2 outputs (000000-1054-146) Accessories (acc. price list Reflector module H (000000-1046-274) Reflector module FI (000000-1046-281) Filter dia. 25 mm and beam splitter (acc. to price list)
Intermediate zoom tube 1.0x. 2.5x cod. (452180-0000-000)
Intermediate tube Pol (with quartz depolarizer) (452184-0000-000)
Intermediate tube for image projection (452181-0000-000)
Binocular phototube 30/25 with sliding prism (100:0/50:50/0:100) (452143-0000-000)
Eyepiece PI 10x/25 Br. (444033-0000-000) Eyepiece PI 10x/25 Br. foc (444034-0000-000) Eyepiece PI 16x/16 Br. (444053-0000-000) Eyepiece PI 16x/16 Br. foc. (444054-0000-000) Eyepiece E - PI 10x/25 Br. foc. (444234-0000-000) Eyepiece E - PI 10x/23 Br. foc. (444235-0000-000)
Binocular tube with two ports 30/25 (100:0/50:50/0:100) (452145-000-000) Axioplan 2 imaging stand, upper part (000000-1087-134) Axioplan 2 imaging MOT stand, upper part (000000-1079-078) High speed shutter FL (000000-1101-354) Reflected-light illuminator HD (000000-1079-047) Reflected-light illuminator FL (000000-1079-073) Antiglare screen (452163-000-000)
Objective nosepiece 7x H W 0.8 (452130-0000-000) Objective nosepiece 6x H DIC W 0.8 (452131-0000-000) Objective nosepiece 6x H D DIC M27 cod. (452136-0000-000) Objective nosepiece 7x H W 0.8 mot. (452137-0000-000) Objective nosepiece 6x H DIC M27 mot. (452138-0000-000) Objective nosepiece 6x H DIC W 0.8 mot. (000000-1067-351) Objective nosepiece 6x Pol W 0.8 cod. (412139-0000-000)
Reflector module H (000000-1046-274) Reflector module D P & C (000000-1099-898) Reflector module DIC (000000-1046-277) Reflector module DIC Rot I (000000-1046-278) Reflector module Pol (000000-1046-279) Reflector module FI (000000-1046-281) Optovar module 2.5x (000000-1046-283) Optovar module 1.6x (000000-1046-283) Optovar module 1.25x (000000-1046-284) Analyzer module D (000000-1050-958) Storing case for reflector modules (000000-1103-997)
Filter slider A (for 3 filters d = 18 mm) (452174-0000-000)
Iris stop slider for incident-light equipment (000000-1062-360) DIC slider (acc. price list) Built-in power supply 12 V/100 W, stabilized 90. 264 V AC/50. 60 Hz/ 225 VA, 2.5. 12 V DC (000000-1079-075) Transmitted-light illumination (452150-0000-000)
Luminous field diaphragm insert D (452160-0000-000) Luminous field diaphragm insert D mot. (000000-1079-076) Filter magazine D (452155-0000-000) Filter magazine D Foto (000000-1088-124) Filter magazine D mot. (000000-1079-077)
Axioplan 2 imaging man stand, lower part (000000-1078-232) Axioplan 2 imaging MOT stand, lower part (000000-1078-233) Axioplan 2 imaging E stand, lower part (000000-1078-234)
Objectives (ICS-Objectives acc. price list)
Antiflexcap for Epiplan-Neofluar 1.25 (44921-0000-000) Antiflexcap for Epiplan-Neofluar 2.5 (4444922-0000-000)
Intermediate ring M 27 on W 0.8 H = 0.94 (444911)-0000-000) Intermediate ring H 0 M 27 on W 0.8 (000000-1095-168)
Intermediate tubes, Illumination, Analyzers
Our local consultants and technicians and the Carl Zeiss customer service all support you in your research with technical and applications aid, whenever you need it. Fast and reliably. Moreover, Carl Zeiss training courses and workshops provide you with added insights into practical areas of microscopy and imaging techniques. In fact, when you add together all the services and support that you get with Axioplan 2 imaging, youll see that it is more than a high-power microscope: Axioplan 2 imaging is your ticket to a powerhouse of knowledge in microscopy that has been built up over the past 150 years.
Convincing from A(bbe)* to Z(eiss)**
Accessible: Clearly laid out: Complete: Convenient: Customized: Efficient: Flexible: Highly resolved: Individual: Infinite: More contrast: Motorized: Open-end: Powerful: Precise: Retrofittable: Sturdy: Time-saving: Versatile: Vibration-free: Zappy:
the diaphragm planes the AxioVisionControl software system solutions from a single source the Push&Click filter cubes the modular system the motorized features the dual video adapter DIC (even with Snarmont) the macro function keys the ICS optics the Light Trap the multi-imaging tube the system architecture the optics the dual-guidance stage the confocal systems the mechanisms the Smart Contrast feature 8 filter positions with 25 mm field of view the SoftStop function the high-speed shutter
* Ernst Abbe (1840 1905), physicist and ingenious researcher, associate of Carl Zeiss, became sole owner in 1889. ** Carl Zeiss (1816 1888), mechanic, opened an optical workshop in 1846, which grew into a global player in optics.
Stand, lower part
Stand, upper part
Manual version Coded version Motorized version
Manual version Motorized version
25 mm field of view Light Trap High-speed shutter (< 20 ms) 8-place reflector turret Push&Click filter cubes Exchangeable field diaphragm Adjusting aid AttoArc 2
Multi-imaging tube Reflector turret Revolving nosepiece Condensers Z motion with 25 nm increments Filter wheels & field diaphragm High-speed shutter External 8-place filter wheel Deflecting mirror for 2 lamps
Trinocular tube Binocular tube with 2 TV ports Binocular tube with 2 TV ports, motorized version Dual video adapter Axiophot 2 photomodule
For further details, please contact:
Rat tongue. Double fluorescence (Alexa 594, DAPI). Plan-Apochromat 40x/1.0 oil. J. Zbaeren, Insel Hospital, Bern
Carl Zeiss Light Microscopy P.O.B. 37030 Gttingen GERMANY Phone : ++Telefax: ++E-Mail: email@example.com www.zeiss.de/micro
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