(2) (3)

4 Associate Professor of Molecular Biology in the Department of Biophysics and Microbiology, University of Pittsburgh, Pittsburgh, PA (10) November, 1964 to June, 1967 Assistant Professor of Biophysics in the Department of Biophysics, University of Pittsburgh, Pittsburgh, PA August, 1961 to October, 1964 Postdoctoral Training: From August, 1961 to June, 1962, Dr. Ho worked with Dr. Jacinto Steinhardt and Professor George Scatchard in the Department of Chemistry, Massachusetts Institute of Technology. From July, 1962 to October, 1964, Dr. Ho worked with Professor David F. Waugh in the Department of Biology, Massachusetts Institute of Technology, Cambridge, MA September, 1960 to August, 1961 Research Chemist, Linde Company, Union Carbide Corporation, Tonawanda, New York. Research work was on the liquid nitrogen temperature preservation of human blood. September, 1957 to August, 1960 Graduate study in the Department of Chemistry, Yale University; Thesis Advisor: Professor Julian M. Sturtevant; Title of Doctoral Dissertation: "A Kinetic Study of Carbonic Anhydrase Action".
ADVISORY COMMITTEES (1) Member of the Ad Hoc Review Committee of the Sickle Cell Disease Contracts of the National Heart, Lung, and Blood Institute, National Institutes of Health, July, 1974 to June 1978. Member of the Biophysics and Biophysical Chemistry B Study Section of the National Institutes of Health, July 1974 to June 1978. Chairman of the Molecular Section on the Analysis and Evaluation of the Sickle Cell Disease, appointed by the Sickle Cell Disease Branch of the National Heart, Lung, and Blood Institute, National Institutes of Health, June 1974. Member of the Advisory Committee of the NMR Facility for Biomedical Studies, Carnegie Mellon University, Pittsburgh, PA, 1965-1986. Member of the Advisory Committee of the Middle Atlantic Regional NMR Facility, University of Pennsylvania, Philadelphia, PA 1973-1982. Chairman of the Advisory Committee of the Stanford Magnetic Resonance Laboratory, Stanford University, Stanford, CA, 1975-1982.

(4) (5) (6)

5 (7) Member of the "Think Tank" - Workshop on the State of Research on Sickle Cell Disease, organized by the Sickle Cell Disease Branch of the National Heart, Lung, and Blood Institute, National Institutes of Health, October 4-6, 1976. Member of the Board of Scientific Councilors for the Developmental Pharmacology Branch, National Institute of Child Health and Human Development, National Institutes of Health, November, 1977. Member and Chairman of the Biotechnology Resources Review Committee, Division of Research Resources, National Institutes of Health, July 1, 1979 to June 30, 1983. Member of the Ad Hoc Advisory Committee for the Regional Instrumentation Facilities Program, National Science Foundation, June 28-29, 1979. Member of the Advisory Committee, Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, January, 1983 to present. Member of the Molecular Biology Advisory Panel, National Science Foundation, January, 1980 to January 1984. Member of the External Advisory Committee of the Division of Atherosclerosis and Lipoprotein Research, Baylor College of Medicine, Houston, Texas, September 1980 to August 1985. Member and Chairman of the National Advisory Committee for the National Biomedical NMR Resource at Madison, University of Wisconsin, Madison, Wisconsin, 1985 to 1990. Member of the Advisory Board of the Graduate Institute of Life Science, National Tsing-Hua University, Hsinchu, Taiwan, 1986 to present. Member of the Cellular and Molecular Basis of Disease Review Committee, National Institute of General Medical Sciences, National Institutes of Health, July 1987 to June 1990. Member of the Council of the International Conference on Magnetic Resonance in Biological Systems, August 1988 to September 1998; member of the Executive Committee, 1996 to 1988. Chairman of the Advisory Committee of the Structural Biology Program, Academia Sinica, Taipei, Taiwan, R.O.C., 1990 to 1998.

(10) (11) (12) (13)

(15) (16)
(19) Member of the Scientific Advisory Committee for the Functional Imaging Research Program, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 1993 to present.

10. 11.

10 Azide Derivatives of Methemoglobins", Proceedings of the National Academy of Sciences, U.S.A. 63, 1403-1409 (1969). 13. C. Ho, D. G. Davis, N. H. Mock, T. R. Lindstrom and S. Charache, "Nuclear Magnetic Resonance Studies of Hemoglobin. IV. The Structure-Function Relationship of Human Adult Hemoglobins A and Chesapeake and its Implication to the Nature of Oxygenation of Hemoglobin", Biochemical and Biophysical Research Communications 38, 779-786 (1970). C. Ho, J. J. Baldassare, and S. Charache, "Electron Paramagnetic Resonance Studies of Spin-Labeled Hemoglobins and Their Implications to the Nature of Cooperative Oxygen Binding to Hemoglobin", Proceedings of the National Academy of Sciences, U.S.A. 66, 722-729 (1970). D. G. Davis, N. H. Mock, T. R. Lindstrom, S. Charache, and C. Ho, "Nuclear Magnetic Resonance Studies of Hemoglobins. V. The Heme Proton Spectra of Human Deoxyhemoglobins A, F, Zrich, and Chesapeake", Biochemical and Biophysical Research Communications 40, 343-349 (1970). J. J. Baldassare, S. Charache, R. T. Jones, and C. Ho, "Electron Paramagnetic Resonance Studies of Spin-Labeled Hemoglobins. II. Roles of Subunit Interactions and of Intermediate Structures in the Cooperative Oxygenation of Hemoglobin and the Results on Hemoglobin Yakima, Hemoglobin J Capetown, and Carboxypeptidases A and B Treated Hemoglobin A", Biochemistry 9, 4707-4713 (1970). R. J. Kurland, R. G. Little, D. G. Davis, and C. Ho, "Proton Magnetic Resonance Study of High-and Low-Spin Hemin Derivatives", Biochemistry 10, 22372246 (1971). D. G. Davis, T. R. Lindstrom, N. H. Mock, J. J. Baldassare, S. Charache, R. T. Jones, and C. Ho, "Nuclear Magnetic Resonance Studies of Hemoglobins. VI. Heme Proton Spectra of Human Deoxyhemoglobins and their Relevance to the Nature of Co-operative Oxygenation of Hemoglobin", Journal of Molecular Biology 60, 101-111 (1971). I. B. E. Norn, C. Ho, and E. F. Casassa, "A Light-Scattering Study of the Effect of Sodium Chloride on the Molecular Weight of Human Adult Hemoglobin", Biochemistry 10, 3222-3229 (1971). T. R. Lindstrom, J. S. Olson, N. H. Mock, Q. H. Gibson, and C. Ho, "Nuclear Magnetic Resonance Studies of Hemoglobins. VIII. Evidence for Preferential Ligand Binding to Chains Within Deoxyhemoglobins", Biochemical and Biophysical Research Communications 45, 22-26 (1971). C. Ho, D. G. Davis, N. H. Mock, and S. Charache, "NMR Studies of Hemoglobins", in Magnetic Resonances in Biological Research, An International

15 Abnormalities, edited by W. S. Caughey, Academic Press, New York, NY, 1978, pp. 179-193. 64. M. P. N. Gent and C. Ho, "Fluorine-19 Nuclear Magnetic Resonance Studies of Lipid Phase Transitions in Model and Biological Membranes", Biochemistry 17, 3023-3038 (1978). M. F. Perutz, J. K. M. Sanders, D. H. Chenery, R. W. Noble, R. R. Pennelly, L. W.M. Fung, C. Ho, I. Giannini, D. Prschke, and H. Winkler, "Interactions Between the Quaternary Structure of the Globin and the Spin State of the Heme in Ferric Mixed Spin Derivatives of Hemoglobin", Biochemistry 17, 3640-3652 (1978). E. A. Pratt, L. W.-M. Fung, J. A. Flowers, and C. Ho, "Membrane-Bound D-Lactate Dehydrogenase from Escherichia coli: Purification and Properties", Biochemistry 18, 312-316 (1979). L. W.-M. Fung, E. A. Pratt, and C. Ho, "Biochemical and Biophysical Studies on the Interaction of a Membrane-Bound Enzyme, D-Lactate Dehydrogenase from Escherichia coli, with Phospholipids", Biochemistry 18, 317-324 (1979). B. A. Manuck and C. Ho, "High-Resolution Proton Nuclear Magnetic Resonance Studies of Histidine-Binding Protein J of Salmonella typhimurium: An Investigation of Substrate and Membrane Interaction Sites", Biochemistry 18, 566-573 (1979). J. M. Sturtevant, C. Ho, and A. Reimann, "Thermotropic Behavior of Some Fluorodimyristoylphosphatidylcholines", Proceedings of the National Academy of Sciences, U.S.A. 76, 2239-2243 (1979). Y.-F. Lam, A. K.-L. C. Lin, and C. Ho, "A Phosphorus-31 Nuclear Magnetic Resonance Investigation of Ingracellular Envirnoment in Human Normal and Sickle Cell Blood Blood 53, 196-209 (1979). G. Viggiano and C. Ho, "Proton Nuclear Magnetic Resonance Investigation of Structural Changes Associated with Cooperative Oxygenation of Human Adult Hemoglobin", Proceedings of the National Academy of Sciences, U.S.A. 76, 3673-3677 (1979). G. Viggiano, N. T. Ho, and C. Ho, "Proton Nuclear Magnetic Resonance and Biochemical Studies of Oxygenation of Human Adult Hemoglobin in Deuterium Oxide", Biochemistry 18, 5238-5247 (1979). I. M. Russu, N. T. Ho, and C. Ho, "Role of the 146 Histidyl Residue in the Alkaline Bohr Effect of Hemoglobin", Biochemistry 19, 1043-1052 (1980). I. M. Russu and C. Ho, "Proton Longitudinal Relaxation Investigation of Histidyl Residues of Normal Human Adult and Sickle Deoxyhemoglobin: Evidence

73. 74.

16 for the Existence of Pregelation Aggregates in Sickle Deoxyhemoglobin Solutions", Proceedings of the National Academy of Sciences, U.S.A. 77, 6577-6581 (1980). 75. S. Takahashi, A. K.-L. C. Lin, and C. Ho, "Proton Nuclear Magnetic Resonance Studies of Hemoglobins M Boston (58E7 HisTyr) and M Milwaukee (67E11 ValGlu): Spectral Assignments of Hyperfine Shifted Proton Resonances and of Proximal Histidine (E7) NH Resonances to the and Chains of Normal Human Adult Hemoglobin", Biochemistry 19, 5196-5202 (1980). C. Ho, Y.-h. Giza, S. Takahashi, K. E. Ugen, P. F. Cottam, and S. R. Dowd, "A Proton Nuclear Magnetic Resonance Investigation of Histidine-Binding Protein J of Salmonella typhimurium: A Model for Transport of L-Histidine Across Cytoplasmic Membranes", Journal of Supramolecular Structure 13, 131-145 (1980). E. Oldfield, R. W. K. Lee, M. Meadows, S. R. Dowd and C. Ho, "Deuterium NMR of Specifically Deuterated Fluorine Spin Probes", Journal of Biological Chemistry 255, 11652-11655 (1980). C. Ho, "1H NMR Investigation of Human Hemoglobins in Solution and Inside Erythrocytes", Bulletin of Magnetic Resonance 2, 25-27 (1980). M. P. N. Gent, P. F. Cottam, and C. Ho, "A Biophysical Study of Protein-Lipid Interactions in Membranes of Escherichia coli: Fluoromyristic Acid as a Probe", Biophysical Journal 33, 211-224 (1981). C. Ho and I. M. Russu, "Proton Nuclear Magnetic Resonance Investigation of Hemoglobins", in Methods in Enzymology, Volume 76, edited by E. Antonini, L. Rossi-Bernardi, and E. Chiancone, Academic Press, NY, 1981, pp. 275312. E. A. Pratt, J. A. Jones, P. F. Cottam, S. R. Dowd, and C. Ho, "Reconstitution of DLactate Dehydrogenase-Deficient Membrane Vesicles using FluorineLabeled Components, An Approach to Investigating Protein-Lipid Interactions in Biological Membranes", Biophysical Journal 37, 101-103 (1982). C. Ho, C.-H. J. Lam, S. Takahashi, and G. Viggiano, "1H NMR Studies Cooperative Oxygenation of Normal Human Adult Hemoglobin", Hemoglobin and Oxygen Binding, edited by C. Ho, W. A. Eaton, J. Collman, Q. H. Gibson, J. S. Leigh, Jr., E. Margoliash, K. Moffat, and W. Scheidt, Elsevier North Holland, Inc., NY, 1982, pp. 141-149. of in P. R.

78. 79.

I. M. Russu, N. T. Ho, and C. Ho, "A 1H NMR Investigation of the Role of Histidyl Residues in the Alkaline Bohr Effect of Normal Human Adult Hemoglobin", in Hemoglobin and Oxygen Binding, edited by C. Ho, W. A. Eaton, J. P.

17 Collman, Q. H. Gibson, J. S. Leigh, Jr., E. Margoliash, K. Moffat, and W. R. Scheidt, Elsevier North Holland, Inc., NY, 1982, pp. 249-256. 84. S. Takahashi, A. K.-L. C. Lin, and C. Ho, "A Proton Nuclear Magnetic Resonance Investigation of Proximal Histidyl Residues in Human Normal and Abnormal Hemoglobins: A Probe for the Heme Pocket", Biophysical Journal 39, 33-40 (1982). I. M. Russu and C. Ho, "Proton Longitudinal Relaxation Investigation of Histidyl Residues in Human Normal Adult Hemoglobin", Biophysical Journal 39, 203-210 (1982). A. Y. Tehrani, Y.-F. Lam, A. K.-L. C. Lin, S. Ferro-Dosch, and C. Ho, "Phosphorus31 Nuclear Magnetic Resonance Studies of Human Red Blood Cells", Blood Cells 8, 245-261 (1982). I. M. Russu, N. T. Ho, and C. Ho, "A Proton Nuclear Magnetic Resonance Investigation of Histidyl Residues in Human Normal Adult Hemoglobin", Biochemistry 21, 5031-5043 (1982). I. M. Russu and C. Ho, "A Proton Nuclear Magnetic Resonance Investigation of Histidyl Residues in Sickle Hemoglobin", Biochemistry 21, 5044-5051 (1982). S. Miura and C. Ho, "Preparation and Proton Nuclear Magnetic Resonance Investigation of Cross-Linked Mixed Valency Hybrid Hemoglobins: Models for Partially Oxygenated Species", Biochemistry 24, 6280-6287 (1982). M. Engelsberg, S. R. Dowd, V. Simplaceanu, B. W. Cook, and C. Ho, "Nuclear Magnetic Resonance Line-Shape Analysis of Fluorine-19-Labeled Phospholipids", Biochemistry 21, 6985-6989 (1982). M. P. Mims, J. S. Olson, I. M. Russu, S. Miura, T. E. Cedel, and C. Ho, "Proton Nuclear Magnetic Resonance Studies of Isonitrile-Heme Protein Complexes", Journal of Biological Chemistry 258, 6125-6134 (1983). M. Cerdonio, S. Morante, S. Vitale, C. Dalvit, I. M. Russu, C. Ho, A. DeYoung, and R. W. Noble, "Magnetic and Spectral Properties of Carp Carbonmonoxyhemoglobin: Competitive Effects of Chloride Ions and Inositol Hexakisphosphate", European Journal of Biochemistry 132, 461-467 (1983). E. A. Pratt, J. A. Jones, P. F. Cottam, S. R. Dowd, and C. Ho, "A Biochemical Study of the Reconstitution of D-Lactate Dehydrogenase-Deficient Membrane Vesicles Using Fluorine-Labeled Components", Biochimica et Biophysica Acta 729, 167-175 (1983).
18 94. A. G. Hunt, V. Simplaceanu, J.-S. Hong, and C. Ho, "Phosphorus-31 Nuclear Magnetic Resonance Investigation of Membrane Vesicles from Escherichia coli", Biochemistry 22, 6130-6134 (1983). C. Ho and I. M. Russu, " H NMR Investigation of the Cooperative Events During the Ligation of Hemoglobin", in Frontiers in Biochemical and Biophysical Studies of Proteins and Membranes, edited by T.-Y. Liu, S. Sakakibara, A. N. Schechter, K. Yagi, H. Yajima, and K. T. Yasunobu, Elsevier Science Publishing Co., Inc., New York, 1983, pp. 309-319. C. Ho, E. A. Pratt, S. R. Dowd, G. S. Rule, V. Simplaceanu, and P. F. Cottam, "Biochemical and Biophysical Studies of Protein-Lipid Interactions in Membranes", in Biochemical and Biophysical Studies of Proteins and Nucleic Acids, edited by T.-B. Lo, T.-Y. Liu and C.-H. Li, Elsevier Science Publishing Co., NY, 1984, pp. 157-168. C. Ho, "Recent Biochemical and Biophysical Studies of Sickle Cell Anemia", in The Proceedings of the Second Symposium on Recent Advances in Biological and Medical Sciences, Academia Sinica, Taipei, Taiwan, R.O.C., 1984, pp. 57-58. I. M. Russu, A. K.-L. C. Lin, S. Ferro-Dosch, and C. Ho, "A Proton Nuclear Magnetic Resonance Investigation of Human Hemoglobin A2: Implications on the Intermolecular Contacts in Sickle Hemoglobin Fibers and on the Bohr Effect of Human Adult Hemoglobin", Biochemica et Biophysica Acta 785, 123-131 (1984). S. Miura and C. Ho, "Proton Nuclear Magnetic Resonance Investigation of CrossLinked Asymmetrically Modified Hemoglobins: Influence of the Salt Bridges on Tertiary and Quaternary Structures of Hemoglobin", Biochemistry 23, 2492-2499 (1984). T. E. Cedel, P. F. Cottam, M.D. Meadows, and C. Ho, "A High-Resolution 1H-NMR Investigation of the Histidine-Binding Protein J of Salmonella typhimurium: Substrate-Induced Conformational Changes", Biophysical Chemistry 19, 279-287 (1984). C. Dalvit, S. Miura, A. De Young, R. W. Noble, M. Cerdonio, and C. Ho, "A HighResolution Proton Nuclear-Magnetic-Resonance Investigation of Carp Hemoglobin: Conformational Differences between Carp and Human Normal Adult Hemoglobins in Solution", European Journal of Biochemistry 141, 255259 (1984). J. F. M. Post, P. F. Cottam, V. Simplaceanu, and C. Ho, "A Fluorine-19 Nuclear Magnetic Resonance Study of 5-Fluorotryptophan-Labeled Histidine-Binding Protein J of Salmonella typhimurium", Journal of Molecular Biology 179, 729-743 (1984).

22 135. Q. Shen, V. Simplaceanu, P. F. Cottam, J.-L. Wu, J.-S. Hong, and C. Ho, "Molecular Genetic, Biochemical and Nuclear Magnetic Resonance Studies on the Role of the Tryptophan Residues of Glutamine-Binding Protein from Escherichia coli", Journal of Molecular Biology 210, 859-867 (1989). Z.-Y. Peng, V. Simplaceanu, S. R. Dowd, and C. Ho, "Effects of Cholesterol or Gramicidin on Slow and Fast Motions of Phospholipids in Oriented Bilayers", Proceedings of the National Academy of Sciences, U.S.A. 86, 8758-8762 (1989). Z.-Y. Peng, N. Tjandra, V. Simplaceanu, and C. Ho, "Slow Motions in Oriented Phospholipid Bilayers and Effects of Cholesterol or Gramicidin: A 19F-NMR T1 Study", Biophysical Journal 56, 877-885 (1989). M. Madrid, V. Simplaceanu, N. T. Ho, and C. Ho, "Effects of Chemical Exchange and Dipole-Dipole Interactions on the Proton Relaxation Rates of Surface Histidyl Residues in Human Hemoglobins", Journal of Magnetic Resonance 88, 42-59 (1990). E. A. Pratt and C. Ho, "NMR and Molecular Genetics as Tools for Investigating Protein Interactions in Membrane Systems", Journal of the Chinese Chemical Society 37, 125-134 (1990). K. E. Sommers, E. Simplaceanu, R. D. Siewers, C. Ho, and P. J. del Nido, "Pyruvate Improves Post-Ischemia Myocardial Function and ATP Recovery: A 31P NMR Study", Surgical Forum XLI, 241-243 (1990). I. M. Russu, S.-S. Wu, K. A. Bupp, N. T. Ho, and C. Ho, "1H and 31P Nuclear Magnetic Resonance Investigation of the Interaction Between 2,3Diphosphoglycerate and Human Normal Adult Hemoglobin", Biochemistry 29, 3785-3792 (1990). O. B. Peersen, E. A. Pratt, H.-T. N. Truong, C. Ho, and G. S. Rule, "Site-Specific Incorporation of 5-Fluorotryptophan as a Probe of the Structure and Function of the Membrane-Bound D-Lactate Dehydrogenase of Escherichia coli: A 19F Nuclear Magnetic Resonance Study", Biochemistry 29, 32563262 (1990). J. A. Detre, A. P. Koretsky, D. S. Williams, and C. Ho, "Absence of pH Changes During Altered Work in the in vivo Sheep Heart: A 31P-NMR Investigation", Journal of Molecular and Cellular Cardiology 22, 543-553 (1990). E. A. Pratt, H.-T. N. Truong, and C. Ho, "NMR and Molecular Genetics as Tools for Investigating Structural and Dynamic Properties of Membrane-Associated Proteins", in Structure and Function of Nucleic Acids and Proteins," edited by F. Y.-H. Wu and C.-W. Wu, Raven Press, New York, 1990, pp. 1-17.

23 145. S. Adler, V. Simplaceanu, and C. Ho, "Brain pH in Acute Isocapnic Metabolic Acidosis and Hypoxia: A 31P-Nuclear Magnetic Resonance Study", Am. J. Physiol. 258, F34-40 (1990). M. R. Busch and C. Ho, "Effects of Anions on the Molecular Basis of the Bohr Effect of Hemoglobin", Biophysical Chemistry 37, 313-322 (1990). D. R. Groebe, A. E. Chung, and C. Ho, "Cationic Lipid-Mediated Co-Transfection of Insect Cells," Nucleic Acids Research 18, 4033 (1990). L. Rossaro, V. Mazzaferro, C. L. Scotti-Foglieni, D. S. Williams, E. Simplaceanu, V. Simplaceanu, A. Francavilla, T. E. Starzl, C. Ho, and D. H. Van Thiel, "Effect of Cyclosporine on Hepatic Energy Status and on Fructose Metabolism after Portacaval Shunt in Dogs as Monitored by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy in vivo", Hepatology 13, 780785 (1991). M. R. Busch, J. E. Mace, N. T. Ho, and C. Ho, "Roles of 146 Histidyl Residue in the Molecular Basis of the Bohr Effect of Hemoglobin: A Proton Nuclear Magnetic Resonance Study", Biochemistry 30, 1865-1877 (1991). H.-T. N. Truong, E. A. Pratt, and C. Ho, "Interaction of the Membrane-Bound DLactate Dehydrogenase of Escherichia coli with Phospholipid Vesicles and Reconstitution of Activity Using a Spin-Labeled Fatty Acid as an Electron Acceptor: A Magnetic Resonance and Biochemical Study", Biochemistry 30, 3893-3898 (1991). H. Farghali, D. S. Williams, E. Simplaceanu, C. Ho, and D. H. Van Thiel, "An Evaluation of the Integrity of the Blood-Testis Barrier by Magnetic Resonance Imaging", Magnetic Resonance in Medicine 22, 81-87 (1991). L. Rossaro, V. Mazzaferro, C. L. Scotti-Foglieni, K. A. Porter, D. S. Williams, E. Simplaceanu, V. Simplaceanu, A. Francavilla, T. E. Starzl, C. Ho, R. Naccarato, and D. H. Van Thiel, "Cyclosporine and Liver Regeneration Studied by in vivo 31P Nuclear Magnetic Resonance Spectroscopy", Digestive Diseases and Sciences 36, 687-692 (1991). P. H. Axelsen, Z. Bajzer, F. G. Prendergast, P. F. Cottam, and C. Ho, "Resolution of Fluorescence Intensity Decays of the Two Tryptophan Residues in Glutamine-Binding Protein from E. coli using Single Tryptophan Mutants", Biophysical Journal 60, 650-659 (1991). W. Zhang, D. S. Williams, and C. Ho, "Longitudinal-Spin-Order Filtered STEAM for the Detection of X Nuclei by Volume-Localized 1H NMR Spectroscopy", Journal of Magnetic Resonance 95, 581-584 (1991). H.-T. N. Truong, E. A. Pratt, G. S. Rule, P. Y. Hsue, and C. Ho, "Inactive and Temperature-Sensitive Folding Mutants Generated by Tryptophan

146. 147. 148.

151. 152.
24 Substitutions in the Membrane-Bound D-Lactate Dehydrogenase of Escherichia coli", Biochemistry 30, 10722-10729 (1991). 156. H. Farghali, M. Sakr, A. Gasbarrini, D. S. Williams, S. R. Dowd, C. Ho, and D. H. Van Thiel, "A Biochemical and 31P-NMR Investigation of the Effect of FK 506 and Cyclosporine Pretreatment on Immobilized Hepatocytes Perifused with Ethanol", Transplantation Proceedings 23, 2805-2808 (1991). K. E. Sommers, A. Ohkado, E. Simplaceanu, B. Wise, A. P. Koretsky, C. Ho, and P. J. del Nido, Pyruvate Improves Postischemic Contractile Function, Energetic Efficiency, and Calcium Regulation", Surgical Forum XLII, 247249 (1991). W. Zhang, D. S. Williams, and C. Ho, "An Approach to Lactate Mapping by SpinFiltered 2D FT MR Imaging", Journal of Magnetic Resonance 96, 631-634 (1992). N. Tjandra, V. Simplaceanu, P. F. Cottam, and C. Ho, "Multidimensional 1H and 15N NMR Investigation of Glutamine-Binding Protein of Escherichia coli", Journal of Biomolecular NMR 2,149-160 (1992). D. R. Groebe, M. R. Busch, T. Y. M. Tsao, F. Y. Luh, M. F. Tam, A. E. Chung, M. Gaskell, S. A. Liebhaber, and C. Ho, "High-Level Production of Human and -Globins in Insect Cells", Protein Expression and Purification 3, 134141 (1992). H. Farghali, L. Rossaro, J. S. Gavaler, D. H. Van Thiel, S. R. Dowd, D. S. Williams, and C. Ho," 31P-NMR Spectroscopy of Perifused Rat Hepatocytes Immobilized in Agarose Threads: Application to Chemical-Induced Hepatotoxicity", Biochimica et Biophysica Acta 1139, 105-114 (1992). C. Ho, "Proton Nuclear Magnetic Resonance Studies on Hemoglobin: Cooperative Interactions and Partially Ligated Intermediates", Advances in Protein Chemistry 43, 153-312 (1992). E. A. Pratt and C. Ho, "Fluorotryptophan as a Nuclear Magnetic Resonance Probe for Investigating Structure and Interactions of D-lactate Dehydrogenase of E. coli", in Advances in Tryptophan Research 1992, Proceedings of the Seventh Meeting of the International Study Group for Tryptophan Research (ISTRY-92), edited by I. Ishiguro, R. Kido, T. Nagastu, Y. Nagamura, and Y. Ohta, Fujita Health University Press, Toyoake, 1992, pp 203-206. L. Rossaro, N. Murase, C. Caldwell, H. Farghali, A. Casavilla, T. E. Starzl, C. Ho, and D. H. Van Thiel, "Phosphorus 31-Nuclear Magnetic Resonance NMR Spectroscopy of Rat Liver During Simple Storage or Continuous Hypothermic Perfusion", Journal of Laboratory & Clinical Medicine 120, 559568 (1992).

25 165. K. E. Sommers, A. Ohkado, E. Simplaceanu, A. P. Koretsky, C. Ho, and P. J. del Nido, "The Role of Magnesium in Postischemic Cardiac Dysfunction", Surgery" 112, 159-165 (1992). L. Rossaro, S. R. Dowd, V. Simplaceanu, R. Naccarato, D. H. Van Thiel, and C. Ho, "Effect of FK506 and Cyclosporins on Model Membranes Studied by Nuclear Magnetic Resonance Spectroscopy", in: Drugs and The Liver, High Risk Patients And Transplantation, edited by D. Galmarini, L. R. Fassati, R. Paoletti, and S. Sherlock, Kluwer Academic Publishers, Dordrecht, The Netherlands, 1993, pp. 177-184. B. K. Fetler, V. Simplaceanu, N. S. VanderVen, I. J. Lowe, and C. Ho, "Design of Pulse Sequences for Solvent Suppression and Uniform Excitation. Application to Observing Hemoglobin in Solution and Inside Red Blood Cells", Journal of Magnetic Resonance B101, 17-27 (1993). T. R. Walsh, J. A. Detre, A. P. Koretsky, E. Simplaceanu, J. M. Halow, P. Rao, L. Makowka, and C. Ho, "Response of Normal and Reperifused Livers to Glucagon Stimulation: NMR Detection of Blood Flow and High-Energy Phosphates", Biochimica et Biophysica Acta 1181, 7-14 (1993). S. R. Dowd, J. M. Sturtevant, V. Simplaceanu, and C. Ho, "31P-NMR and Calorimetric Studies of the Low-Temperature Behavior of Three 19FLabeled Dimyristoylphosphatidylcholines", Journal of Physical Chemistry 97, 2946-2951 (1993). T.-J. Shen, N. T. Ho, V. Simplaceanu, M. Zou, B. N. Green, M. F. Tam, and C. Ho, "Production of Unmodified Human Adult Hemoglobin in Escherichia coli", Proceedings of the National Academy of Science, U. S. A. 90, 8108-8112 (1993). G. S. Rule, N. Tjandra, V. Simplaceanu, and C. Ho, Assignment Strategies for 15N-1H Correlated Spectra of Large Proteins in Solution", Journal of Magnetic Resonance B102, 126-128 (1993). M. C. Schrader, C. J. Eskey, V. Simplaceanu, and C. Ho, "A Carbon-13 Nuclear Magnetic Resonance Investigation of the Metabolic Fluxes Associated with Glucose Metabolism in Human Erythrocytes", Biochimica et Biophysica Acta 1182,162-178 (1993). M. C. Schrader, V. Simplaceanu, and C. Ho, "Measurement of Fluxes through the Pentose Phosphate Pathway in Erythrocytes from Individuals with Sickle Cell Anemia by Carbon-13 Nuclear Magnetic Resonance Spectroscopy", Biochimica et Biophysica Acta 1182,179-188 (1993). J. D. Fontenot, N. Tjandra, D. Bu, C. Ho, R. C. Montelaro, and O. J. Finn, Biophysical Characterization of One-, Two-, and Three-Tandem Repeats of
26 the Human Mucin (muc-1) Protein Core", Cancer Research 53, 5386-5394 (1993). 175. T.-c. Yeh, W. Zhang, S. T. Ildstad, and C. Ho, "Intracellular Labeling of T-Cells with Superparamagnetic Contrast Agents", Magnetic Resonance in Medicine 30, 617-625 (1993). PMID8259062 Z.-Y. Sun, H.-T. N. Truong, E. A. Pratt, D. C. Sutherland, C. E. Kulig, R. J. Homer, S. M. Groetsch, P. Y. Hsue, and C. Ho, "A 19F-NMR Study of the Membrane-Binding Region of D-Lactate Dehydrogenase of Escherichia coli",Protein Science 2, 1938-1947 (1993). H. Farghali, D. S. Williams, P. Caraceni, A. B. Borle, A. Gasbarrini, J. Gavaler, H. L. Rilo, C. Ho, and D. H. Van Thiel, "Effect of Ethanol on Energy Status and Intracellular Calcium of Sertoli Cells: A Study Using Immobilized Perfused Cells", Endocrinology 133, 2749-2755 (1993). W. Zhang and C. Ho, "Lactate Mapping with Full Sensitivity by Spin-Filtered NMR Imaging", Journal of Magnetic Resonance B103, 120-127 (1994). J. D. Fontenot, N. Tjandra, C. Ho, P. C. Andrews, and R. C. Montelaro, Structure and Self Assembly of a Retrovirus (FeLV) Proline Rich Neutralization Domain", Journal of Biomolecular Structure and Dynamics 11, 821-836 (1994). H. Farghali, H. Rilo, W. Zhang, V. Simplaceanu, J. S. Gavaler, C. Ho, and D. H. Van Thiel, "Liver Regeneration after Partial Hepatectomy in the Rat: Sequential Events Monitored by 31P-NMR Spectroscopy and Biomedical Studies", Laboratory Investigation 70, 418-425 (1994). C.-D. Hsiao, Y.-J. Sun, J. Rose, P. F. Cottam, C. Ho, and B.-C. Wang, "Crystals of Glutamine-Binding Protein in Various Conformational States," Journal of Molecular Biology 240, 87-91 (1994). C. Ho and J. R. Perussi, "Proton Nuclear Magnetic Resonance Studies of Hemoglobin", Methods in Enzymology 232, 97-139 (1994). A. W. Hing, N. Tjandra, P. F. Cottam, J. Schaefer, and C. Ho, An Investigation of the Ligand-Binding Site of Glutamine-Binding Protein of Escherichia coli Using REDOR NMR", Biochemistry 33, 8651-8661 (1994). H.-W. Kim, T.-J. Shen, D. P. Sun, N. T. Ho, M. Madrid, M. F. Tam, M. Zou, P. F. Cottam, and C. Ho, Restoring Allosterism with Compensatory Mutations in Hemoglobin", Proceedings of the National Academy of Science U.S.A. 91, 11547-11551 (1994).

31 223. C. Ho and J. A. Lukin, Hemoglobin: Cooperativity in Protein-Ligand Interactions, in Embryonic Encyclopedia of Life Sciences, Macmillan Reference Ltd., London, England, www.els.net (2000). S. Kanno, P. C. Lee, Z. Zhang, C. Ho, B. P. Griffith, L. L. Shears, II., and T. R. Billiar, Attenuation of Myocardial Ischemia/Reperfusion Injury by Superinduction of Inducible Nitric Oxide Synthase, Circulation 101, 27422748 (2000). PMID10851213 V. Simplaceanu, J. A. Lukin, T.-Y. Fang, M. Zou, N. T. Ho, and C. Ho, ChainSelective Isotopic Labeling for NMR Studies of Large Multimeric Proteins: Application to Hemoglobin, Biophysical Journal 79, 1146-1154 (2000). PMID10920044 M. J. Rao, A. Malavalli, B. N. Manjula, R. Kumar, M. Prabhakaran, D. P. Sun, N. T. Ho, C. Ho, R. L. Nagel, and A. S. Acharya, Interspecies Hybrid Hemoglobin S: Complete Neutralization of Val-6() Dependent Polymerization Reaction by Pig -Chains, Journal of Molecular Biology 300, 1389-1406 (2000). PMID10903876 O. Dym, E. A. Pratt, C. Ho, and D. Eisenberg, The Crystal Structure of D-Lactate Dehydrogenase, A Peripheral Membrane Respiratory Enzyme, Proceedings of the National Academy of Sciences USA 97, 9413-9418 (2000). PMID10944213 Y. Zhang, S. J. Dodd, K. S. Hendrich, M. Williams, and C. Ho, MRI Detection of Rat Renal Transplant Rejection by Monitoring Macrophages Infiltration, Kidney International 58, 1300-1312 (2000). PMID10972694 J. A. Lukin, V. Simplaceanu, M. Zou, N. T. Ho, and C. Ho, NMR Reveals Hydrogen Bonds Between Oxygen and Distal Histidines in Oxyhemoglobin, Proceedings of the National Academy of Sciences USA 97, 10354-10358 (2000). PMID10962034 S. Kanno, P. C. Lee, S. J. Dodd, M. Williams, B. P. Griffith, and C. Ho, A Novel Approach with Magnetic Resonance Imaging Used for the Detection of Lung Allograft Rejection, Journal of Thoracic and Cardiovascular Surgery 120, 923-934 (2000). PMID11044319 T.-Y. Fang, V. Simplaceanu, C.-H. Tsai, N. T. Ho, and C. Ho, An Additional HBond in the 12 Interface as the Structural Basis for the Low Oxygen Affinity and High Cooperativity of a Novel Recombinant Hemoglobin (L105W), Biochemistry 39, 13708-13718 (2000). PMID11076510 C.-H. Tsai, T.-Y. Fang, N. T. Ho, and C. Ho, Novel Recombinant Hemoglobin, rHb (N108Q), With Low Oxygen Affinity, High Cooperativity, and Stability 13719-13729 (2000). Against Autoxidation, Biochemistry 39, PMID11076511
C. L. Robertson, K. S. Hendrich, P. M. Kochanek, E. K. Jackson,, J. A. Melick, S. H. Graham, D. W. Marion, D. S. Williams, and C. Ho, Assessment of 2Chloroadenosine Treatment After Experimental Traumatic Brain Injury in the Rat Using Arterial Spin-Labeled MRI: A Preliminary Report, Acta Neurochirurgica 76 (Suppl), 187-189 (2000). PMID11450003 D. Barrick, N. T. Ho, V. Simplaceanu, and C. Ho, Distal Ligand Reactivity and Quaternary Structure Studies of Proximally Detached Hemoglobins, Biochemistry 40, 3780-3795 (2001). PMID11300758 P. M. Kochanek, K. S. Hendrich, C. L. Robertson, D. S. Williams, J. A. Melick, C. Ho, D. W., Marion, and E. K. Jackson, Assessment of the Effect of 2Chloroadenosine on Cerebral Blood Flow in Normal Rats Using Arterial Spin-Labeled MRI, Magnetic Resonance in Medicine 45, 924-929 (2001). PMID11323821 K. S. Hendrich, P. K. Kochanek, J. A. Melick, J. K. Schiding, K. D. Statler, D. S. Williams, D. W. Marion, and C. Ho, Cerebral Perfusion During Anesthesia With Fentanyl, Isoflurane, or Pentobarbital in Normal Rats Studied by Arterial Spin-Labeled MRI, Magnetic Resonance in Medicine 46, 202-206 (2001). PMID11443729 S. Kanno, Y.-J. Lin Wu, P. C. Lee, S. J. Dodd, M. Williams, B. P. Griffith, and C. Ho, Macrophage Accumulation Associated with Rat Cardiac Allograft Rejection Detected by Magnetic Resonance Imaging Using Ultrasmall Superparamagnetifc Iron Oxide Particles, Circulation 104, 934-938 (2001). PMID11514382 S. Kanno, Y.-J. L Wu, P. C. Lee, T. R. Billiar, and C. Ho, Angiotensin-Converting Enzyme Inhibitor Preserves p21 and Endothelial Nitric Oxide Synthase Expression in Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats, Circulation 104, 945-950 (2001). PMID11514384 C.-H. Tsai, S. C. Larson, T.-J. Shen, N. T. Ho, G. W. Fisher, M. F. Tam, and C. Ho, Probing the Importance of the Amino-Terminal Sequence of the - and Chains to the Properties of Normal Adult and Fetal Hemoglobins, Biochemistry 40, 12169-12177 (2001). PMID11580292 D. Yang, Q. Ye, M. Williams, Y. Sun, T. C.-C. Hu, D. S. Williams, J. M. F. Moura, and C. Ho, USPIO-Enhanced Dynamic MRI: Evaluation of Normal and Transplanted Rat Kidneys, Magnetic Resonance in Medicine 46, 1152-1163 (2001). PMID11746582 J. A. Lukin and C. Ho, Hemoglobin, in Wiley Encyclopedia of Molecular Medicine edited by T. E. Creighton, Volume 3, John Wiley & Sons, Inc., New York, pp. 1573-1579, 2002.

33 242. Q. Ye, D. Yang, M. Williams, D. S. Williams, C. Pluempitiwiriyawej, J. M. F. Moura, and C. Ho, In-Vivo Detection of Acute Rat Renal Allograft Rejection by MRI with USPIO Particles, Kidney International 61, 1124-1135 (2002). PMID11849467 S. J. Dodd, D. S. Williams, C. Olson, A. C. Silva, A. P. Koretsky, and C. Ho, An Open Transverse z-Gradient Coil Design for Magnetic Resonance Imaging, Review of Scientific Instruments 73, 2208-2210 (2002). C.-K. Chang, V. Simplaceanu, and C. Ho, Effects of Amino Acid Substitutions at 131 on the Structure and Properties of Hemoglobin: Evidence for the Communication Between 11 and 12 Subunit Interfaces, Biochemistry 41, 5644-5655 (2002). PMID11969426 S. J. Dodd and C. Ho, Short Planar Gradient Coils for MR Microscopy Using Concentric Return Paths, Journal of Magnetic Resonance 156, 1-9 (2002). PMID12081437 C.-H. Tsai and C. Ho, Recombinant Hemoglobins with Low Oxygen Affinity and High Cooperativity, an invited review, Biophysical Chemistry 98, 15-25 (2002). PMID12128186 Y. Yuan, V. Simplaceanu, J. A. Lukin, and C. Ho, NMR Investigation of the Dynamics of Tryptophan Side-Chains in Hemoglobins, Journal of Molecular Biology 321, 863-878 (2002). PMID12206767 M. Q. Huang, Q. Ye, D. S. Williams, and C. Ho, MRI of Lungs Using Partial Liquid Ventilation with Water-in-Perfluorocarbon Emulsions, Magnetic Resonance in Medicine 48, 487-492 (2002). PMID12210913 P. M. Kochanek, K. S. Hendrich, C. E. Dixon, J. K. Schiding, D. S. Williams, and C. Ho, Cerebral Blood Flow at One Year after Controlled Cortical Impact in Rats: Assessment by Magnetic Resonance Imaging, Journal of Neurotrauma 19, 1029-1037 (2002). PMID12482116 Y. Cheng, T.-J. Shen, V. Simplaceanu, and C. Ho, Ligand Binding Properties and Structural Studies of Recombinant and Chemically Modified Hemoglobins Altered at 93 Cysteine, Biochemistry 41, 11901-11913 (2002). PMID12269835 J. A. Lukin and C. Ho, Nuclear Magnetic Resonance of Hemoglobins, in Hemoglobin Disorders: Molecular Methods and Protocols, edited by R. L. Nagel, Methods in Molecular Medicine, Volume 82 Humana Press, Totowa, New Jersey, 2002, pp. 251-269. C.-H. Tsai, V. Simplaceanu, N. T. Ho, T.-J. Shen, D. Wang, T. G. Spiro, and C. Ho, Site Mutations Disrupt Inter-helical H-Bonds (14W-67T and 15W-72S)
34 Involved in Kinetic Steps in the Hemoglobin RT Transition without Altering the Free Energies of Oxygenation, Biophysical Chemistry 100, 131-142 (2002). PMID12128186 253. J. A. Lukin, G. Kontaxis, V. Simplaceanu, Y. Yuan, A. Bax, and C. Ho, Quaternary Structure of Hemoglobin in Solution, Proceedings of the National Academy of Sciences, U.S.A. 100, 517-520 (2003). PMID12525687

38 Absence and Presence of an Allostertic Effector, Biochemistry 46, 99739980 (2007). PMID17691822 291. X.-J. Song, V. Simplaceanu, N. T. Ho, and C. Ho, Effector-Induced Structure Fluctuation Regulates the Ligand Affinity of an Allosteric Protein: Binding of Inositol Hexaphosphate Has Distinct Dynamic Consequences for the T and R States of Hemoglobin, Biochemistry 47,4907-4915 (2008). PMC2493540 P. H. Mills, Y.-J. L. Wu, C. Ho, and E. T. Ahrens, Sensitive and Automated Detection of Iron-Oxide-Labeled Cells Using Phase Image CrossCorrelation Analysis, Magnetic Resonance Imaging 26, 618-628 (2008). NIHMS54555 Q. Ye, Y. L. Wu, L. M. Foley, T. K. Hitchens, D. F. Eytan, H. Shirwan, and C. Ho, Longitudinal Tracking of Recipient Macrophages in a Rat Chronic Cardiac Allograft Rejection Model With Noninvasive Magnetic Resonance Imaging Using Micrometer-Sized Paramagnetic Iron Oxide Particles, Circulation 118, 149-156 (2008). PMC2613640 H.-H. Chang, J. M. Moura, Y. L. Wu , and C. Ho, Automatic Detection of Regional Heart Rejection in USPIO-Enhanced MRI, IEEE Transactions on Medical Imaging 27, 1095-1106 (2008). PMC2562708 D. H., Maillett, V. Simplaceanu, T.-J. Shen, N. T. Ho, J. S. Olson, and C. Ho, Interfacial and Distal Pocket Mutations Exhibit Additive Effects on the Structure and Function of Hemoglobin, Biochemistry 47, 10551-10563 (2008). PMC2692483 L. M. Foley, T. K. Hitchens, J. A. Melick, H. Bayir, C. Ho, and P, M. Kochanek, Effect of Inducible Nitric Oxide Synthase on Cerebral Blood Flow After Experimental Traumatic Brain Injury in Mice, Journal of Neurotrauma 25, 299-310 (2008). PMID18373480 M. D. Manole, L. M. Foley, T. K. Hitchens, P. M. Kochanek, R. W. Hickey, H. Bayir, H. Alexander, C. Ho, and R. S. B. Clark, Magnetic Resonance Imaging Assessment of Regional Cerebral Blood Flow After Asphyxial Cardiac Arrest in Immature Rats, Journal of Cerebral Blood Flow & Metabolism 1-9 (2008). PMC2613172 C. Brinegar, Y.-J. L. Wu, L. M. Foley, T. K. Hitchens, Q. Ye, C. Ho, and Z.-P. Liang, Real-Time Cardiac MRI Without Triggering, Gating, or Breath Holding, 30th Annual International IEEE Conference, IEEE-EMBS, Vancouver, British Columbia, Canada, August 20-24, 2008, pp. 3381-3384. PMC2793087 Y. L. Wu, Q. Ye, K. Sato, L. M. Foley, T. K. Hitchens, and C. Ho, Non-invasive Evaluation of Cardiac Allograft Rejection by Cellular and Functional MRI,
39 Journal of the American College of Cardiology: Cardiovascular Imaging 2, 731-741 (2009). PMC2752220 300. G. A. Bermejo, M.-P. Strub, C. Ho, and N. Tjandra, Determination of the Solution Bound Conformation of an Amino-acid Binding Protein by NMR Paramagnetic Relaxation Enhancement: Use of a Single Flexible Paramagnetic Probe with Improved Estimation of Its Sample Space, Journal of the American Chemical Society 131, 9532-9737 (2009). PMC2720827 C. Brinegar, H. Zhang, Y.-J. L. Wu, L. M, Foley, T. K. Hitchens, Q. Ye, D. Pocci, F. Lam, C. Ho, and Z.-P. Liang, Real-Time Cardiac MRI Using Prior SpatialSpectral Information, 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS), Minneapolis, Minnesota, September 2-6, 2009, pp. 4383-4386. PMC2800039 L. M. Foley, T. K. Hitchens, C. Ho, K. L. Janesko, J. A. Melick, H. Bayir, and P. M. Kochanek, Magnetic Resonance Imaging Assessment of Macrophage Accumulation in Mouse Brain After Experimental Traumatic Brain Injury, Journal of Neurotrauma 26, 1509-1519 (2009). PMC 2822809 X. Jia, C. Li, Y. Sun, A. A. Kassim, Y. L. Wu, T. K. Hitchens, and C. Ho, A DataDriven Approach to Prior Extraction for Segmentation of Left Ventricle in Cardiac MR Images, Proceedings IEEE International Symposium on Biomedical Imaging (ISBI), pp. 831-834, July 2009. G. A. Bermejo, M.-P. Strub, and C. Ho, Ligand-free Open-closed Transitions of Periplasmic Binding Proteins: The Case of Glutamine-binding Protein, Biochemistry 49, 1893-1902 (2010). PMC2831130 C. Ho and Y. Yue, Haemoglobin: Cooperativity in Protein-Ligand Interactions (version 2.0) in Encyclopedia of Life Sciences, John Wiley & Sons, Ltd., http://www/els.net [DOI Chichester 10.1002/9780470015902.a0001345.pub2], April 2010. K. L. Campbell, J. E. E. Roberts, L. N. Watson, J. Stetefeld, A. M. Sloan, A. V. Signore, J. W. Howatt, J. R. H. Tame, N. Rohland, T.-J. Shen, J. J. Austin, M. Hofreiter, C. Ho, R. E. Weber, and A. Copper, Substitutions in Woolly Mammoth Hemoglobin Confer Biochemical Properties Adaptive for Cold Tolerance, Nature Genetics 42, 536-540 (2010). L. M. Foley, T. Kevin Hitchens, B. Barbe, F. Zhang, C. Ho, G. R$. Rao, and E. M. Nemoto, Quamtitative Temporal Profiles of Penumbra and Infarction During Permanent Middle Cerebral Artery Occlusion in Rats, Journal of Translational Stroke Research 1 (Issue 3), in press (2010). DOI 10.1007/s12975-010-0032-6], July 19, 2910.