CM1950_en_Brochure_Neu.qxd:CM1950_en_Brochure_Neu.qxd

28.08.2008

13:00 Uhr

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Leica CM1950
THE Disinfecting Clinical Cryostats

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Leica CM1950 Platform
THE cryostats for high quality sectioning, user safety and efficient workflow
The Leica CM1950 platform is the latest innovation in a long line of distinguished cryostats manufactured by Leica Microsystems. Leica designed and developed the new family of cryostats based upon users feedback and their requirements for high quality sections, user safety, and efficient laboratory workflow. The brightl cabinet is allow effici ple specim

High Quality Sectioning

High precision microtome with stepper motor object feed Motorized sectioning (optional) New stable specimen clamp system New flat specimen discs for high specimen stability New rapid-freezing shelf design New effective object cooling system (optional)

User Safety

Learn quickly and work confidently using the externally located, self-explanatory controls with easily recognizable symbols.
For an extra measure of safety, the inside of the instrument features an ozone-free UVC disinfection system that is certified to be effective against most bacteria, virus, and fungi, even at cold temperatures.
UVC disinfection AgProtect antimicrobial nanosilver surface coating New disposable blade holder CE Debris-free, frost-free enclosed microtome assembly Leicas Section Waste Removal System (optional) Spacious cabinet with rounded edges inside and out

Efficient Workflow

Increased safety for the user and others in the immediate work area: Leicas AgProtect antimicrobial nanosilver surface coating deters the propagation of infectious agents on the outside of the instrument.

Protect

UVC disinfection at any time and temperature Organized work space inside and out Frost-free, encapsulated microtome assembly Leicas Section Waste Removal System with easily-accessible, concealed filter system (optional) Easily recognizable control symbols Visible operating parameters at all times
To protect the laboratory environment from contamination, a multi-filter system (including HEPA filter) removes particulates and cleans the air exhausted from the optional Section Waste Removal System
Liquid condensate collected in an eas ble waste container

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rightly lit, ergonomic cryoet is spacious enough to efficient handling of multiecimens.
Store tools, glass slides, staining containers, etc. in a recessed area on top of the cryostat to have these items immediately at hand.
For extra ease of use and consistent sectioning of all specimen types, an optional sectioning motor drive is available.
nsate is safely easily accessiainer.
Leica Design by Werner Hlbl

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THE cryostats for high quality sectioning
Obtain high quality sections through:
NEW: CryoZone blade holder and anti-roll guide cooling object temperature Maintaining theofidealmicrotome and bladefor specific specimens holder system Great stability the the stepper motor object feed Extreme accuracy of cutting stroke (optional) Consistent, motor-driven aid (optional) Vacuum-assisted sectioning exact stepper motor object feed. High precision microtome with
CryoZone the cool way to improve section quality The CryoZone system controls a zone of uniformly cool air in the critical areas of the cryochamber. This cool air circulates around the specimen, knife and anti-roll guide to create the optimal conditions for consistent, high-quality sectioning.

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Efficient specimen loading is achieved via single lever operation that provides maximum clamping force independent of individual user strength.Direct contact cooling through the (optional) actively cooled object head facilitates rapid changes of specimen temperatures to provide the best conditions for sectioning various specimen types.
High specimen stability is achieved by adherence to deeply grooved specimen discs. Specimens freeze quickly because the pre-cooled discs feature a large back surface that fully contacts the freezing shelf with integrated Peltier element.
Superior sectioning of routine, hard, and/or large samples is achieved using a choice of knife/blade holders as well as the new dove tail mounting system that provides increased stability and extended working distance. The new CE holder for high and low profile disposable blades evenly distributes clamping pressure across the entire length of the blade, which provides vibration-free sectioning.
The new CN and CNZ knife holders are available for steel or tungsten carbide knives (optional accessories).
The optional vacuum sectioning aid achieves time-saving section preparation and reduces section curling.

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THE cryostats for the highest user safety
Histology laboratorires continue to express major concerns about biohazard exposure and non-ergonomic work practices. As a result of their feedback, the Leica CM1950 family of cryostats is designed to provide maximum protection from these risks at all stages of the sectioning process. exposed surfaces Certified UVC disinfectionatofcold temperatures and air within the cryochamber AgProtect antimicrobial nanosilver outside surfaces of the cryostat coating on the Optional Section Waste Removal System with a series of three filters Ergonomic exterior cabinet design with rounded edges and comfortable working height Optional ergonomic chair and foot rest Leicas built-in UVC disinfection system provides certified protection from infectious material and minimizes the risk of contamination in the cryochamber. Scientific studies prove that surface disinfection from bacteria, viruses, and fungi can be achieved with UVC light. Certification proving the effectiveness of UVC disinfection against a range of microorganisms can be downloaded from www.leica-microsystems.com/cm1950_safety, which includes detailed information on the test procedure and the efficacy of UVC disinfection. To efficiently reduce the propagation of infectious agents on the outside surfaces of the cryostat, the Leica CM1950 family features an antimicrobial nanosilver surface coating, called AgProtect. Silver (Ag) ions are well documented for their ability to reduce bacterial growth.

Section waste is easily removed by using Leicas optional Section Waste Removal System during trimming with the magnetized nozzle, or during extensive cleaning with the flexible hose. Solid waste is collected in a concealed primary filter system and air is filtered through a HEPA filter for added safety in the laboratory environment. Liquid condensate is collected in a condensate bottle for safer disposal.

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The new disposable blade holder CE with integrated, colored safety guard and blade removal aid makes blade handling safer. For users who prefer using the brush technique during sectioning, the palm rest can be used instead of the anti-roll guide.
The ergonomic design of the Leica CM1950 protects the user from sharp edges and helps prevent work-related musculoskeletal disorders (MSD) and repetitive strain injury (RSI). Rounded edges allow the user to adopt a comfortable working position. Bright, glare-free illumination helps reduce eye fatigue.
For individual user comfort, the Leica chair and height adjustable foot rest are available as optional accessories.

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THE cryostats for highly efficient workflow
The best workflow for efficient operation is achieved by:
the optional, color-coded specimen Simple specimen identification usingwith clearly defined zero position discs Fast, efficient specimen orientation and low profile blades blade holder for both high Disposableenclosed microtome assembly Frost-free, drive for specimen sectioning Optional motordisinfection at low temperatures does not produce condensation or Certified UVC waste, is effective at low temperatures, and can be terminated at any contaminated time to process urgent cases
Easy for the multi users: the selfexplanatory, single-function keys and easily readable LEDs help prevent operating errors, facilitate cryosectioning, and make scrolling through multiple menus unnecessary. The control panels are comfortably positioned in the armrests to eliminate the need to reach into the cold chamber to use or adjust settings.
Tools, glass slides, staining containers, etc. can be stored in a recessed area on top of the cryostat to keep them immediately at hand.

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Sectioning tools/aids etc. can be conveniently placed on the optional, movable shelf.
The specimen discs can be pre-cooled in the optional internal storage container, and then easily organized and transported.

Optional color coded specimen discs help identify specimens coming from multiple sources. The specimen disc features a visual specimen orientation aid to enable precise orientation.
A clearly defined zero-position allows faster specimen alignment prior to sectioning and easy realignment if the specimen is removed from the object head.
With the new disposable blade holder CE blade exchange is simple and fast. The holders lateral displacement function allows the use of the full length of the blade. Small and large sections are easily lifted from the large, horizontal pressure plate.

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Leica CM1950 Technical Specifications
The Leica CM1950 basic instrument is equipped with CryoZone technology, UVC Disinfection, specimen orientation and AgProtect antimicrobial nanosilver coating. An individual, tailor-made instrument can be configured by use of the various options available: Actively cooled object head (double compressor) Vacuum Section Waste Removal System Motorized Sectioning A wide range of accessories 30 or 180 minutes, user selectable
UVC surface disinfection: Microtome Section thickness range:

Setting values:

Trimming range:
Clinical: Setting values: Research: Setting values:
Maximum specimen size: Total specimen feed: Vertical specimen stroke: Specimen retraction: Specimen orientation: Electric coarse feed: Slow: Fast:
1 to 100 m 1.0 m 5.0 m in 0.5 m steps 5.0 m 20.0 m in 1.0 m steps 20.0 m 60.0 m in 5.0 m steps 60.0 m 100.0 m in 10.0 m steps m 10 m, 20 m, 30 m, 40 m m 1,0 m 10,0 m in 1,0 m steps 10,0 m 20,0 m in 2,0 m steps 20,0 m 50,0 m in 5,0 m steps 50,0 m 100,0 m in 10,0 m steps 100,0 m 600,0 m in 50,0 m steps 50 x 80 mm 25 mm 59 mm 20 m or off 8 (x-, y-axis), 360 rotation of specimen disc 300 m/s, in 20 m steps 900 m/s
Cryochamber Temperature range: Specimen cooling (optional) Temperature range: Defrosting of specimen head: Cryochamber defrosting Automatic cryochamber defrosting:
0C to -35C at ambient temperature of 20 C
10 to 50 C manual defrost
Automatic shut off defrost:
programmable, (hot gas defrost), selectable time, 1 defrost in 24 h or manual hot gas defrost, defrost time: 12 minutes at 5 C chamber temperature

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Quick-freeze shelf Minimum temperature: Number of freezing stations: Defrost: Peltier element Number of freezing stations: Maximum temperature difference: Power draw General information Operating temperature range: Relative humidity: Storage humidity: Dimensions and Weights Width (without handwheel): Width (with handwheel): Depth (cabinet only): Height (total): Working height (armrest): Weight: Weight (with vacuum): Weight (with motor): Weight (with motor and vacuum):

down to 42 C, at chamber temp. 35C 15 + 2 manual hot-gas defrost
K, at chamber temp. of 35 C 1900 VA
18C to 35C max. 60%, non-condensing < 60%
700 mm 835 mm 850 mm 1215 mm 1025 mm 165 kg 183 kg 185 kg 193 kg
Choice of blade or knife holders: Stable blade holder base with segment arc for lateral displacement of the blade. Disposable blade holder insert CE for high and low profile blades with 2 anti-roll guides with 50 and 100 m spacer with glare minimizing glass insert, colored safety guard and blade removal help. Palm rest for brush technique. Stable knife holder base for knife holder CN or knife holder CNZ. Knife holder CN for reusable steel knives Knife holder CNZ for reusable steel or tungsten carbide knives with central pressure plate for full usage of the knife. Wide range of accessories available on request. Technical specifications subject to change without prior notice. As confirmed by the successful c_CSA_us certification, the Leica CM1950 has been designed and manufactured in compliance with US, Canadian and IEC requirements. State-of-the-art development, manufacturing and quality control procedures certified under DIN EN ISO 9001 ensure highest quality and reliability.

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With the user, for the user Leica Microsystems
Leica Microsystems operates internationally in four divisions, where we rank with the market leaders.

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The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems customers at the leading edge of science.
The statement by Ernst Leitz in 1907, with the user, for the user, describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.

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Leica Microsystems GmbH HRB 5187 09/2008

Unpacking and Installation
To ensure proper function of the instrument, it must be set up with a minimum distance on all sides from walls and furniture (see Site Requirements, p. 20) The instrument may only be transported in an upright or slightly inclined position. To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down. Before connecting to the power supply system, please check if the local voltage complies with the power rating specified on the name plate of the instrument (see also Technical data)! Never connect the instrument to a power socket that does not have a protective conductor terminal. Length of power cable: up to 3.5 m extension possible: no
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return into its original position. Any condensation on electrical parts that has formed due to temperature differences during transport must be allowed to dry completely. Failure to comply with this can cause severe damage to the instrument!
When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. 2

Fig. 2

Opening the packaging
1. Remove the 3 screws (1) on each of the two sides of the box (7) at the top. 2. Remove the 2 screws on each of the 4 hinges (2) on the front and rear side of the box.
Do NOT remove the screws of the hinges on the top lid! Cover = ramp
3. Remove 1 screw (3) between the two hinges on the front and rear side of the box. 4. Remove the 3 screws (4) at the bottom on all four sides of the box. 5. Of the two vertical rows of screws (5), remove one on either side (which side does not matter) entirely.

Fig. 3

Removing the packaging

Fig. 4

1. Remove the cover (17) and place it on the floor with the foam parts facing downwards. - The cover will be used as a ramp later. 2. Remove the 4 foam parts (6) by pulling them upwards. 3. Open the box (7) on its side hinges like an accordion partition and remove it. 4. Remove the accessories carton (8) and operating manual on the front of the instrument. 5. Pull off the dust cover (9) by pulling upwards. 6. Take off the foam strip (10) on the bottom rear.

Ramp assembly

Fig. 5
1. After the foam strip (10) is removed, 2 screws (12) are visible on the baseplate. 2. Swing out the cover hinges (2) at the front and rear. 3. Attach the cover (17) from the rear on the transport pallet (11). The notches (13) of the hinges (2) must be pointing towards the instrument. 4. Move the cover towards the left so that the notches (13) of the hinges slide beneath the heads of the screws (12).

6 oclock position Fig. 7

To lock the handwheel, press the lever (1) outward. Continue turning the handwheel slowly until the grip is in the upper or lower position and the handwheel is locked. Press the lever fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably. To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.

Fig. 8

Centering of the handwheel (motorized instruments only) Pull out the handwheels handle and position it in the middle of the handwheel. The handle automatically engages in this position.
An important safety device on the cryostat is the centering of the handwheel for motorized instruments. Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold.

Fig. 9

Cleaning, Disinfection Turning the Instrument Back On
It is not necessary to remove the microtome for disinfection.
The instrument has been designed for UV disinfection! Spray disinfection with Leica Cryofect is also possible, thanks to the special insulation of the microtome. (Cryofect is not available in all countries!)
Remove section waste after EVERY sectioning operation and BEFORE changing specimens. Remove the section waste using a paper towel soaked in Cryofect or an alcohol-based disinfectant) or remove the waste using the extraction nozzle (optional). Do not start the disinfection until after folding the section stretcher to the side! Each new specimen is a potential source of contamination.
When disinfecting the instrument, take appropriate protective measures (gloves, mask, protective clothing, etc.). When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer. The integrated glass anti-roll guide of the blade holder CE, CN and CN-Z can be cleaned either with acetone or alcohol. Dispose of waste liquid according to the waste disposal regulations! Do not use external heaters for drying the cryochamber. This can cause damage to the cooling system! Do not turn the instrument on before the cryochamber is completely dry! Frost formation! All components removed from the cryostat must be carefully dried before returning them to the cryochamber! The front panel and the slit cover of the microtome must be completely dry before turning on the instrument!

To ensure proper function of the instrument, it must be set up while maintaining a minimum distance from walls and furniture.
Distance to walls and furniture, calculated from the cabinet: - rear: 15 cm - right side: 30 cm - left side: 15 cm No heat dissipating appliances around.
High room temperatures and excessive air humidity affect the cooling capacity of the cryostat and lead to ice forming in the instrument!
Plug the instrument into power sockets with ground only. Power cable length: up to 3.5 m Extendable: no

6. Installation

6.2 Transport to the Site
First, check if the location meets the conditions specified in "Site requirements". Transport the instrument to the desired location. Observe the following:
The instrument must be transported in an upright position or slightly tilted (max. 30)! When tilting the instrument 2 people must counterbalance from the front side to prevent the instrument from falling down and causing severe injury!
When transporting the instrument on wheels (2) grip the cabinet only at the marked locations ( ). To do so, unscrew the adjustable feet using the No. 13 open-end wrench (when subsequently transporting the instrument on castors, screw the feet back in as far as they will go). To ensure a secure upright position at its intended location, align both adjusting feet (1).
Before transport or relocation, remove the filter bag from the chamber. If you fail to do this, the filter bag will thaw, then freeze solid when the instrument is reconnected. When subsequently removed, the filter will be destroyed, causing section waste to get into the bacteria filter. (Refer to p. 26.)

1 Fig. 12

2 (covered)
When not using the extraction for a long period, tightly close the opening for the extraction hose using the silicone stopper (5, page 28) included in the scope of delivery.
Transport with a fork lift The instrument can be transported with a fork lift.
To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down.
At the installation site, unscrew the adjustable feet (1) with the openend wrench No. 13 (see Fig. 12). This is absolutely necessary for a stable stand.

Total width with handwheel Fig. 13

Assembling the Handwheel

Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold.

Fig. 14

Insert the pin (1) of the handwheel shaft into the hole (2) of the handwheel. Tighten the screw (4) using the No. 6 Allen key. Place the protective cap on the screw (4). To dismount, proceed in reverse order.
6.3.1 Locking/Unlocking the Handwheel
Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold!
6 oclock position Fig. 15
Always lock the handwheel prior to manipulating the knife or specimen, changing the specimen, or taking a break.

Fig. 16

To lock the handwheel, move its handle to the 12 or 6 oclock position. Press the lever (1) fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably. To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.
Centering the Handwheel (Optional)
An important safety device on the cryostat is the feature for centering the handwheel in motorized sectioning mode.
To do so, pull out the handwheels handle and position it in the middle of the handwheel. The handle automatically engages in this position.

Fig. 17

6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor)
The footswitch dummy must be installed on the outer right side of the instrument (see page 16, General overview) if no footswitch (optional) is used. If the red LED in the E-STOP field in control panel field 3 is illuminated, either: emergency-stop function active, or footswitch dummy (optional footswitch) connected incorrectly or not at all.

Fig. 18

Connection to Power Supply System
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return into its original position. Furthermore, any condensation that has formed during this time due to temperature fluctuations must be allowed to dry completely. Failure to comply with this can cause severe damage to the instrument! During the start-up of the compressor the nominal voltage must not drop below the values specified in the "Technical Data". Please note that the compressor requires a start-up current between 25 and 35 A. Therefore, the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. Failure to comply with the above will cause severe damage to the instrument!

Check mains voltage and mains frequency to comply with the specification on the type plate. Do not connect any other consumers to this electric circuit.
Never connect the instrument to a power socket that does not have a protective conductor terminal.
Only for instruments sold in Japan Selecting the frequency
After unpacking the instrument and setting it up at its intended location, use the lever (1) to select the frequency corresponding to the conditions of the existing power system.

Power plug Fig. 19

6.5 Installing Accessories/Inserting Chamber Accessories
To install the optional footrest, unscrew the screws (1) using the No. 3 Allen key provided.
6.5.1 Installing the Adjustable Footrest (Optional)
When installing the holder (2), ensure that the cutout faces downwards so that the support (3) can be hooked in.
Attach the holders (2) for the footrest on the left and right to the front wall of the housing. To do so, use the Allen screws you used earlier. Ensure that the screws are tight. Hook the footrest (3) into the installed holder according to individual requirements (height). Once installed, the user can adjust the height of the footrest at any time by relocating it (3) to the desired height on both sides in the holder (2).

Fig. 20

6.5.2 Installing the Storage Systems (Optional)
For reasons of accessibility, the (optional) storage system must always be installed first.

Fig. 21

To do so, remove the insert (1), place the frame (2) in front of the bore and tighten the screws/ washers on the cryostat housing using the No. 4 Allen key. Afterwards, insert the insert (1) into the frame and fold it up.
6.5.3 Shelf, Movable (Optional)
Attach the rod for the shelf to the inner front side of the cryostat housing using the provided screws (1) and the No. 3 Allen key, then attach the caps (3). (The rear side of the movable shelf has white plastic screws (2) that prevent the interior of the chamber from being scratched.) Now hook the movable shelf into the guide rod.

3 Fig. 22

6.5.4 Inserting the Section Waste Tray
Before installing the knife/ blade holder base, insert the section waste tray with the cutout (a) facing the user.

Fig. 23

6.5.5 Installing the Heat Conductor Block, Stationary (Optional)
The holder (1, Fig. 24) of the heat conductor block is screwed to the left housing wall using the No. 4 Allen key provided. (Preferably, begin with the bottom screw. Then, rotate the holder upwards (see arrow) and insert and tighten the top screw).

If emergency stop has been activated, the cutting mode must be selected again. The red LED in the E-STOP field indicates either: emergency-stop function active, or footswitch dummy (optional footswitch) connected incorrectly or not at all. The mechanical handwheel brake is enabled when the yellow LED is lit in the M-STOP field.
Motor speed controller (0-100%)
Hold the button to section at maximum speed. Release the button to continue sectioning at the previously selected speed (see controller, above). Switch from slow speed range to fast speed range: while switching on the instrument, press and hold down the Vmax button. Fig. 34 Speed ranges Slow: 0-50 strokes/min Fast: 0-85 strokes/min Vmax: 85-90 strokes/min
The handwheel must also be locked when working on the specimen head. Press to brake the handwheel electronically (LED lit) specimen stops in lower (6 oclock) position. Can be used in any position. 1. Press the buttons at the same time to start motorized sectioning. 2. To end the sectioning process, press RUN/STOP, ENABLE or BRAKE. - Specimen head stops at bottom (for BRAKE, automatic electronic braking takes place). 3. For motorized sectioning, does not have to be unlocked; continue working by pressing both the RUN/STOP and ENABLE keys. 4. If, during manual work using handwheel, you have braked using BRAKE also use BRAKE to release!

Sectioning modes

The instrument can be used in manual and motorized modes. The following settings are available: Single stroke (SINGLE) or continuous stroke (CONT.) in automatic mode, as well as ROCK (sectioning using handwheel) in manual mode.
When switching the instrument on, no operating mode is active for safety reasons.
Disinfection Duration - 30min Duration - 180 min UVC key - to activate / deactivate the disinfection cycle and/or to acknowledge interruption of a disinfection cycle. To start disinfection the sliding window must be completely closed. Press UVC key once briefly to start the 30 min. mode Press UVC key once for a longer time (approx. 4 sec.) to start the 180 min. mode

Fig. 35

The germicidal UV lamp is a simple and safe disinfection device which considerably reduces the risk of infection. Nevertheless, UV light disinfection cannot replace chemical disinfection of the cryochamber at regular intervals. UV light disinfection is effective only on those surfaces that are directly illuminated.

Specimens and section waste must be thoroughly removed from the cryochamber first (e. g. using the optional vacuum extractor, or a paper towel soaked with Cryofect or alcoholic disinfectant). Before UV disinfection, move the anti-roll guide to the side to allow complete disinfection.
Leica Cryofect is not available in all countries.
Opening the sliding window cancels the disinfection cycle. Press the UVC key to acknowledge this.
When the keypad lock is activated (via the key button) the UV lamp can be shut off only by opening the glass, as the UV keys are locked. The cancellation can be acknowledged only if the keypad lock is disabled. Only then can the UV lamp be switched back on.
Working with the Instrument
Preparing Cutting Tools, Specimen Discs and Preparation Aids
The knives are extremely sharp! Handle with care! Never try to catch a falling knife!
Place working materials such as the blade box or knives (in the knife case), brush, pincers or preparation needles and, where applicable, specimen discs into the cryostat chamber. The necessary tools and preparation aids can be precooled on the (optional) movable shelf, making them available at all times in a convenient position for the user. In addition, specimen discs can be precooled and stored in the storage system (see page 26, Fig. 21).
For installation of knife/blade holder and installation in the chamber, refer to optional accessories on page 50).

Fig. 36

Turning the Instrument On
The instrument must be switched on at least 5 hours before the planned use.
The circuit breaker is used as power switch. The switch must be in the top position for switching on and in the bottom position for switching off. The switch must be accessible without obstruction. Close the sliding window.

Fig. 37

To avoid frost formation always put the cover on the quick freeze shelf. Always cover the quick freeze shelf during breaks and overnight.
8.3 Configuring the Parameters

Turn the lamp on or off.

This button activates or deactivates the manual defrost of the cooling chamber, specimen head or freeze shelf. (For more detailed handling instructions, refer to the chapter on "Working with the Instrument" on p. 39 of this Operating Manual.) Pressing the key button for approx. 5 sec. locks the entire keyboard (the LEDs in the clock go out). Pressing the key button briefly, then the "-" key in the specimen head control panel field, switches off the specimen head. Pressing the key button briefly, then the "+" key in the specimen head control panel field, switches the specimen head back on. Programming the temperature of the cryochamber The temperature of the cryochamber is set and indicated on the control panel marked with the cryostat symbol. The actual temperature is the standard indication. Briefly pressing the "+" or "-" button displays the target temperature. Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the chamber temperature continuously. The actual value will be indicated 5 seconds after finishing the programming.

Refer to page 52 for a table with guide values. The temperature values given there are based on long-term experience, but are intended solely as guide values, as any tissue may require particular adjustments.
Programming the specimen temperature Select the desired temperature of the specimen. The specimen temperature is set and indicated on the control panel marked with the specimen head symbol. The actual temperature is the standard indication. Briefly pressing the "+" or "-" button displays the target temperature. Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the specimen temperature continuously. The actual value will be indicated 5 seconds after finishing the programming.
Specimen temperature - "Max-Cool" function The snowflake button for the Max-Cool function is in the panel with the specimen head symbol. Pressing the button sets the lowest possible specimen head temperature (-50 C) as the target temperature. The instrument adjusts the maximum low temperature of the specimen head, i.e. -50 C. Push the snowflake button again to stop the "Max-Cool" function. The temperature adjusts to the value programmed prior to activating the "Max-Cool"-function. Alternate flashing of "LL" and the actual temperature indicates activation of the Max-Cool function. Setting the time The clock time is set with the +/- buttons in the control panel marked with the clock symbol. To do so, set the current time using the "+" or "-" button below the small clock symbol. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the time continuously (auto-repeat function).
Programming the defrost cycle
Set the beginning of the automatic defrost cycle. The automatic defrost cycle takes place once within 24 hours. It is set with the "+"/"-" buttons on the right of the panel with the clock. symbol. The two buttons are marked by a melting snowflake Briefly press the "+" or "-" button for indication of the beginning of the defrost cycle which has actually been set. At the same time, the LEDs between the indication of hours and minutes are flashing. To change the beginning of the defrost cycle in steps of 15 minutes, push the "+" or "-" key. When the "+" or "-" button is pushed for more than 1 s, the defrost time value increases or decreases continuously.

Press the key button, then the "+" button = specimen head on Press the key button, then the "-" button = specimen head off
Entering the trimming thickness
To switch from a trimming section thickness for research applications (1-600 m) to a thickness for clinical applications (10, 20, 30 or 40 m), press and hold down the TRIM/SECT key while switching on the instrument.
Press the TRIM/SECT button. TRIM mode is active if the LED at the top right is illuminated. Set the desired trimming thickness using the "+" or "-" button in control panel field 2. (Refer to page 32 for adjustable sequence of steps) Entering the section thickness Press the TRIM/SECT button. SECT mode is active if the LED at the bottom right is illuminated. Set the desired section thickness using the "+" or "-" button in control panel field 2. (Refer to page 32 for adjustable sequence of steps) Switching the retraction on or off in manual sectioning mode Press the TRIM/SECT button for approx. 3 sec. The LED in control panel field 2 displays on or oFF. You can switch by pressing the "+" or "-" button. "Retraction on" means a specimen retraction of 20 m in manual mode.
During motorized sectioning, the retraction value is speeddependent and cannot be changed by the user.
Working with the Precooled Cryostat
Lock the handwheel in the top (12 oclock) position. Cut the specimen to size outside of the cryostat. Select the precooled specimen disc, cover it with freezing compound, then attach and orient the specimen. Attach the specimen disc and specimen to the Peltier position on the freeze shelf. Activate the Peltier element and wait until the specimen is completely frozen.
Specimens that have been frozen on the Peltier element are often too cold and split apart during sectioning. Allow time for the specimens to become acclimated.

8.4.1 Preparatory Work

Insert the specimen disc into the specimen head. Fold the anti-roll guide away and to the side. Insert a precooled knife or blade.
Before using them for the first time, degrease new knives using acetone or alcohol.
Approach the specimen with the knife or blade holder: To do so, open the clamping lever of the base, approach the specimen and close the lever again. Open the lever of the orientation. Orient the specimen (move it into a favorable position relative to the knife/blade) and close the lever again. Approach the knife or blade holder using the coarse feed buttons and gentle movements of the handwheel.

Condensation on anti-roll plate and knife during cleaning Anti-roll plate damaged after adjustment
Brush, forceps and/or cloth too warm.
Anti-roll plate too high above the knife edge. The adjustment was carried out in the direction of the knife. Temperature incorrect for the tissue cut. Knife profile inappropriate for the specimen cut. Ice buildup on the knife back. Handwheel speed not uniform. Knife not clamped tightly enough. Specimen disc not clamped tightly enough. Cryocompound applied to cold specimen disc; specimen detached from disc after freezing.
Replace anti-roll plate. Be more careful next time!

Thick-thin sections

Select correct temperature. Use knife with different profile (c or d). Remove ice. Adapt speed. Check knife clamping. Check disc clamping. Apply cryocompound to warm disc, mount specimen and freeze.

Problem Causes

Blunt knife. Inappropriate section thickness. Incorrect clearance angle. Microtome not dried thoroughly enough. Dried specimen. Tissue sticks to the anti-roll plate Anti-roll plate is too warm or incorrectly positioned. Fat on the corner or edge of anti-roll plate. Anti-roll plate not correctly fixed. Rust on the knife. Anti-roll plate too warm.
Use different part of the knife. Select correct section thickness. Set correct angle. Dry microtome. Prepare new specimen. Cool down anti-roll plate, or reposition correctly. Remove fat from anti-roll plate. Fix correctly. Remove rust. Cool down anti-roll plate.
Flattened sections curl up when anti-roll plate is folded up Sections tear
Temperature too low for the tissue cut. Blunt part, dirt, dust, frost or rust on the knife. Top edge of the anti-roll plate damaged. Hard particles in the tissue. Dirt on knife back. Power plug not properly connected. Defective fuses, or circuit breaker has triggered.
Increase temperature and wait. Remove cause. Replace anti-roll plate. --Clean. Check if properly connected. Replace fuses, or switch circuit breaker back on. If not possible, call technical service. Apply concentrated alcohol to the contact point.

Cryostat non-operational

Specimen disc cannot be removed
Moisture on the underside cause the specimen to freeze to the freezing shelf or specimen head.

No or insufficient refrigeration of the cryochamber Sliding window collects condensation No or insufficient refrigeration of the specimen Lamp does not work
Cooling system or electronic drive defective.

Call technical service.

Air humidity and room temperature too high. Cooling system or electronic drive defective.
Comply with the requirements for the installation site. Call technical service.
Lamp defective. Switch defective.
Check lamp and replace it, if necessary. Call technical service. Replace UV tube following the manufacturers instructions. Contact technical service and follow the instructions given.
Both disinfection LEDs flash alternately
UV radiation provided by UV tube no longer sufficient. Image of an open-ended wrench appears due to fault to be rectified.
Temperature Selection Chart (in minus C)
Chamber temperature -15 C to -20 C -10 C -15 C Specimen head temperature -11 C -20 C off to -15 C -20 C A*: off to -20 C E*: -20 C

Tissue type Spleen Liver

Intestine

-10C -15 C

A: -20 C E: -20 C to -30 C
-15 C Ovaries -10 C -15 C Fallopian tubes -10 -15 C Kidney -10 C -15 C -20 C Muscle Skin with fat Hard fat Stomach -18 C to -20 C -19 C -19 C -10 C -15 C Brain *A = blocked, *E = complete -15 C
off to -20 C E: -20 C off to -15 C E: -20 C off to -15 C -20 C A: off to -15 C -20 C -15 C -32 C to -40 C -21 C to -25 C -20 C off to -15 C -10 C, *E
The temperature values given above are based on long-term experience, however, these are only approximate values, as any tissue may require particular adjustments.

11. Optional Accessories

11.1 Ordering Information
Specimen disc 20mm assembly.. Specimen disc 30 mm assembly.. Specimen disc 40 mm assembly.. Specimen disc 55 mm assembly.. Specimen disc 80 x 50 mm, assembly.. O-ring blue (10 pieces), 20 mm... O-ring red (10 pieces), 20 mm... O-ring blue (10 pieces), 30 mm... O-ring red (10 pieces), 30 mm... O-ring blue (10 pieces), 40 mm... O-ring red (10 pieces), 40 mm... O-ring blue (10 pieces), 55 mm... O-ring red (10 pieces), 55 mm... Blade holder base complete.. Blade holderextension CE-BB, complete.. Insert for low-profile blades for blade holder CE, complete. Finger rest, complete... 047740387 Anti-roll plate 70 mm - 50 m spacer.. Anti-roll plate 70 mm -100m spacer.. Anti-roll plate 70 mm -150m spacer.. Glass insert 70 mm, polished.. Knife holder base assembly... Knife holder CN assembly... Knife support CN short... Knife support CN... Knife holder attachment CNZ, assembly... Anti-roll plate assembly glass 50mm.. Heat and cold extractor, assembly... Cold extractor, assembly... Heat extractor, assembly.. 0477 43126

In case of visible pollution (such as dust), clean the air inlet opening (Fig. 10, p. 18) of the condenser on the bottom right-hand side of the instrument using a brush, broom or extraction cleaner in the direction of the louvers.
Do not turn the instrument on before the cryochamber is completely dry! Frost formation! The front panel and the slit cover of the microtome must be completely dry before turning on the instrument! Dry all parts completely before reinserting them in the cryochamber!

12.2 Changing Fuses

In case of power supply faults, please contact an authorized Leica service technician immediately.
Do not carry out any repairs on your own as this will invalidate the warranty. Repairs may only be carried out by qualified service engineers authorized by Leica.
12.3 Replacement of the Lamp/UVC Lamp
Turn the instrument off with the automatic power fuse and pull the power plug, before replacing the lamp! If the lamp is broken, it must be replaced by the technical service, as the replacement involves a high risk of injury. Beware of the metallic mercury in the UV lamp; handle it carefully and dispose of it properly.
A UVC lamp has an estimated service life of approx. 9,000 hours. Each on/off switching cycle reduces the lamp life by approx. one hour plus burning time (30 minutes or 180 minutes respectively). When both disinfection indicator lights are blinking alternately in control panel field 1, the UVC lamp must be replaced.
Instructions on how to replace the UVC lamp are provided together with the replacement lamp (spare part).
Slightly lift the sliding window (1) holding it by the grip (2) and pull it out to the front.
Removal of the lamp The lamp is mounted behind a glare shield and therefore not visible. Touch the lamp for better orientation. Lightly tilt the fluorescent tube down to the left and pull it out of the clip (3). Hold the lamp with both hands and pull it to the left out of the holder (4).
Only use lamps of the same specification!

2 Fig. 68

Installation of the new lamp
Type: OSRAM DULUX L 18W/ 840.
Hold the lamp in the correct mounting position as shown and push it to the left until it engages in the holder.

Fig. 70

13. Decontamination Certificate (master)
Dear Customer, Any product which is to be returned to Leica Microsystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Leica accepts that it is not possible to decontaminate for CJD and its variants and in this instance following current customer safety guidelines is acceptable. Equipment exposed to CJD samples cannot be returned to Leica Microsystems for repair. This certificate, duly completed, must be placed in the unit prior to packaging or handed over to the service engineer. Packages will not be opened nor servicing commenced until the Company or service engineer have received a satisfactory certificate. Should returned goods be considered a hazard by the Company, they will be returned immediately to the customer at his/her expense. NB: Microtome knives must be in boxes.

In addition, the following in-house standards were applied: DIN EN ISO 9001: 2000 Quality management system - requirements. Leica Biosystems Nussloch GmbH Postfach 1120 D-69222 Nussloch October 11, 2007
.. Anne De Greef-Safft President Biosystems Division

Warranty

Warranty and Service
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively. Technical service information If you require technical service or replacement parts, please contact your Leica sales representative or dealer who sold the product. Please provide the following information: Model name and serial number of the instrument. Location of the instrument and name of the person to contact. Reason for the service call. Date of delivery.
Shutdown and disposal of the instrument The instrument or parts of the instrument must be disposed of in compliance with the local laws. Dispose of UV tube separately.
People's Republic of China