Leica TCS SPE

Spectacular Imaging! Technical Documentation
Spectacular Imaging Easy to Achieve A Reliable System Affordable Excellence
The high resolution spectral confocal Leica TCS SPE is an integrated system, optimized for target applications in small research groups and multi-user environments. A reliable partner providing spectacular results easily and yet affordably.
2 G 3 G 4 G 5 G 6 G 7 G 8 G 9 G 10 G 11 G 12 G 13 G 14 G 15 16
Research microscope A Inverted B Upright Confocal scan head Supply unit, including three or four lasers and power supply, PC Galvometer stage Microscope control unit Camera port Digital camera EL6000 fluorescence illumination control Smart move microscope control Computer table Microscope table Computer mouse Keyboard Control monitor

Specifications

Scan Head Scanner Method Confocal channels Scanner Sequential scan Channels Range (min - max) Scan formats Image depth Spectral detection Type Spectral resolution Bandwidth [pixel] [pixel] [bit] true confocal 1 Galvo, [x,y] yes 1- 8, sequential multiplexing 1282 - 20482 128, 256, 512, 1024, or 12, switchable yes continuously variable 5 nm 430 - 750

Resolution

Spectral Detection

[nm] [nm]

Detector
Detector Detector type Detector connection Illumination
1 ultra high dynamic PMT direct laser

Pinhole

Pinhole type Range (min - max) Pinhole adjustment Control

[m] [%]

motorized, variable 35 - - 100 automated via GUI

Beam Splitter

Type Beam splitter wavelength ND-splitter for TLD Beam splitter exchange

[nm] [%]

new high performance dichroics 405/532, 488/633 30/70 automated
Zoom type Zoom range Zoom increment 2D: x-y x-z x-t 3D: x-y-z x-y-t x-y- Band 4D: x-y-z-t
continuously variable 1x - 16x 0.1 yes yes yes yes yes yes yes yes

Scan Modes

FOV Speed
Field of view (diagonal) Speed mode Line speed Line speed range max @ 1282 standard @ 5122 min @ 20482
15.5 uni-directional 800 400, 600, 800 3.0 1.0 0.36
[Hz] [Hz] [f/s] [f/s] [f/s]

Supply Unit

Laser type Laser Laser excitation wavelength Excitation attenuation Excitation attenuation control Range
solid state max 4 405, 488, 532, 635 AOTF automated 0 - 100

Computer

Integrated PC Processor HD-Size Operating system
yes Intel Pentium-M 120 XP-embedded

Interfaces

Ethernet USB FireWire Parallel Serial Type Monitor resolution Monitor size [Pixel]

1 optical 1280 x 1024 19

Mouse Monitor

Power Supply

Power supply integration Type Voltage range Z-focus Z-resolution Z-focus device, other
yes autoselect 100 - 240 galvanometer stage 10 nm no DM2500 CSQ DM5500 CSQ DMI 4000 CSQ LEI, LIF, TIFF, AVI, JPEG yes yes yes yes yes (yes) yes

Z-Drive

Microscope

upright inverted

Software

Export Modules

Formats Acquisition 3D-visualisation Acqusition, time series Averaging Time lapse Multi point time lapse Colocalization Humidity Operating temperature Guaranteed stability [%] [C]

Environment

Ratings

10 - - 30 23C +/- 2C

System Overview Leica TCS SPE
TCS SPE VIS with HC-Optics

Solid State Laser:

R: SS 18 mW 635 nm G: SS 10 mW 532 nm B: SS 10 mW 488 nm V: SS 25 mW 405 nm

Scan Head

Standard Accessories:
Fast Z-Movement SuperZ Galvo Focus SPE 10 SuperZ Galvo Inserts

Fluo-Illuminat EL6000

Itensity Shuter
Supply Unit with integrated PC RGB 14 13

Shutter

Insert for SuperZ, standard

Solid State Laser

Microscopes:
DM2500 CSQ VIS Lasersafe 6x fluo BF incl. Optical Outfit and Basic Objective Set
DM5500 CSQ VIS Lasersafe 5x fluo BF incl. Optical Outfit and Basic Objective Set
DMI 4000 CSQ VIS Lasersafe 6x fluo BF incl. Optical Outfit and Basic Objective Set
201 TCS SPE w. DM2500 CSQ VIS Additional Options: Transmitted Light DetectorKits
TLD for DM 101 TLD for DMI
401 TCS SPE w. DM5500 CSQ VIS
501 TCS SPE w. DMI4000 CSQ VIS

Motorized xy-Stage Kits

Digital Camera Kit

Climate Chamber Kit

400 Climate chamber for DMI 4000 CSQ (1) Including Heating control unit
251 Optical outfit DIC for DM2500 CSQ 7
200 DM Basic 3-Plate stage motorized kit
300 Leica DFC350 FX incl. C-Mount Adapter x 0,63 (1)
Including Temp control unit
551 Optical outfit DIC for DM5500 CSQ
451 Optical outfit DIC for DMI 4000 CSQ
201 DMI Basic 3-Plate s tage motorized kit
Note: (1) Available after May 2006
TCS SPE V-VIS with ACS-Optics
Scan Head Input / Output Equipment External light source HXP120 burner Liquid light guide Adapter TFT Monitor 19" Keyboard, Mouse Supply Unit with integrated PC RGBV 14 13
1 DM2500 CSQ V-VIS Lasersafe 6x fluo BF incl. Optical Outfit and Basic Objective Set
DM5500 CSQ V-VIS Lasersafe 5x fluo BF incl. Optical Outfit and Basic Objective Set
DMI 4000 CSQ V-VIS Lasersafe 6x fluo BF incl. Optical Outfit and Basic Objective Set
210 TCS SPE w. DM2500 CSQ V-VIS
410 TCS SPE w. DM5500 CSQ V-VIS
510 TCS SPE w. DMI4000 CSQ V-VIS

SuperZ Galvo Inserts

411 Insert for SuperZ, universal 410 Insert for SuperZ, rotatable Option Insert for SuperZ Galvo Focus w/o heating Option CO2 cover for insert for SuperZ Galvo Focus

Tables

Software LAS AF TCS SPE
202 LAS AF SPE Live Data Mode
190 Microscope table for upright and inverted systems, passive.
203 LAS AF SPE Colocalization 204 LAS AF SPE Deconvolution 205 LAS AF SPE 3D Visualization 207 LAS AF SPE Motion Spy 208 LAS AF SPE Dye Finder

190 Computer table

Room Requirements
Power Supply Power supply integration Type Voltage range Power consumption Independent circuits Frequency Fuse: standard Humidity (noncondensing) Operating temperature Guaranteed stability Confocal unit, max Microscope, max System Static floor load [V] [VA] [no.] [Hz] [A] [%] [C] yes autoselect 100 - 1 50/10 - - 30 23C +/- 2C 120 150

visible radiation:

Load Capacity and Weight

[kg] [kg] [kg] [kg/m2]

1450 mm

900 mm

100 mm

1200 mm

720 mm

700 mm

2100 mm

1400 mm

www.leica-microsystems.com/Confocal_Microscopes
Copyright Leica Microsystems CMS GmbH Am Friedensplatz Mannheim Germany 2006 Tel. +49 (0)621-Fax +49 (0)621-LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. Printed on chlorine-free bleached paper. II/06/SK

TCS SPE II_en_Data sheet_New.qxd:Datenblatt_TCS_SPE

20.07.2010

13:45 Uhr

Seite 1

Leica TCS SPE
Spectacular Imaging! Technical Documentation

Seite 2

Spectacular Imaging Easy to Achieve Built-in Reliability Affordable Excellence
The new high resolution spectral confocal Leica TCS SPE is an integrated system for life science research. A reliable partner, providing spectacular results easily.

Seite 3

Research microscope A DM2500 CSQ B DMI4000 CSQ C DM5500 CSQ Confocal scan head Supply unit, including 3 or 4 lasers and power supply SuperZ Galvo stage Microscope control unit Camera port Digital camera EL6000 fluorescence illumination control Smart move microscope control Computer table Anti-vibration microscope table Computer mouse Keyboard Monitor Workstation Control panel

Seite 4

Specifications Leica TCS SPE
Scan Head Scanner Method Confocal channels Scanner Sequential scan Channels Range (min - max) Scan formats Image depth Spectral detection Type Spectral resolution Bandwidth [pixel] [pixel] [bit] True Confocal Point Scanner 1 Galvo, [x,y] yes 1 8, sequential multiplexing 128, 256, 512, 1024, or 12, switchable yes continuously variable 5 nm 430 - 750

Resolution

Spectral Detection

[nm] [nm]

Detector
Detector Detector type Detector connection Illumination
1 ultra high dynamic PMT direct laser

Pinhole

Pinhole type Range (min - max) Pinhole adjustment Control

[m] [%]

motorized, variable automated via GUI

Beam Splitter

Type Beam splitter wavelength (532nm laser systems) Beam splitter wavelength (561nm laser systems) ND-splitter for TLD/ Beam splitter exchange

[nm] [nm] [%]

high performance dichroics 405/488/633 405/532 405/488/561/635 488/561 30/70 automated continuously variable 1x 58x 0.1 xt xyz; xyz xyt; xzt xy; xz xyzt; xzyt xyt; xzt xyz xyzt 15.5
Zoom type Zoom range Zoom increment 2D 3D Time Lambda Time Time & lambda Lambda Time & lambda [mm]

Scan Modes

Field of view (diagonal)
Speed mode Line speed range max @ 1282 bi-directional standard @ 5122 min @ 20482

[Hz] [f/s] [f/s] [f/s]

uni-, bi-directional 400, 600, 800, bi: 1200, 1600 6.1 2.2 0.8

13:46 Uhr

Seite 5

Supply Unit

Laser type Laser Laser excitation wavelength Excitation attenuation Excitation attenuation control Range
solid state max 4 405, 488, 532, 561, 635 AOTF, direct modulation automated 0 100

Computer

External PC Processor Memory HD-Size Operating system USB FireWire Ethernet Serial Mouse Keyboard

[GB] [GB]

yes Intel Core i500 Windows 4(2x 1394a, 3x 1394b) optical yes

Interfaces

Monitor
Graphics resolution Monitor

[Pixel]

1280 x 1024, 2560 x 1600 (optional) 1x 19'', 2x 19'' (optional), 30'' (optional)

Power Supply

Power supply integration Type Voltage range Z-focus Z-resolution Z-focus device, other Leica DFC360 FX Digital Camera Leica DFC310 FX Digital Camera Leica Roper CoolSNAP HQ2 Camera Leica Hamamatsu 9100-02 Camera upright inverted
yes autoselect 100 - 240 galvanometer stage 10 [nm] microscope drive DM5500 yes yes yes yes DM2500 CSQ DM5500 CSQ DMI4000 CSQ LIF, TIFF, AVI, JPEG, MOV yes yes yes yes yes yes yes yes yes 23C +/- 2C

Z-Drive

Digital Cameras Support

Microscope Types

Software

Export Modules

Formats LAS AF SPE Live Data Mode LAS AF SPE Colocalization LAS AF SPE Deconvolution LAS AF SPE 3D Visualization LAS AF SPE Dye Finder LAS AF SPE Core Review Software LAS AF SPE Camera Advanced LAS AF SPE FRAP LAS AF High Cont. Screening Autom. Humidity Operating temperature Guaranteed stability

Environment

Ratings

[%] [C]

Seite 6
System Overview Leica TCS SPE

Confocal Systems

Confocal Scanning Units
Solid State Laser: R: SS 18 mW 635 nm Y: SS 20 mW 561 nm G: SS 10 mW 532 nm B: SS 10 mW 488nm V: SS 25 mW 405 nm Supply Unit R Y B V PC Insert for SuperZ, standard SuperZ Galvo Focus SPE 9a EL 6000 External H Scan Head Scanning Accessories: Fast Z Movement SuperZ Galvo Inserts Microscope Access Fluo Equipment

Liquid Adap Ocula

Solid State Laser

Keyboard, Mouse

Confocal Prepared Microscopes
9 DM 2500 CSQ VIS Lasersafe 5x fluo BF incl. Optical Outfit DM 5500 CSQ VIS Lasersafe 5x fluo BF incl. Optical Outfit DMI 4000 CSQ VIS Lasersafe 5x fluo BF incl. Optical Outfit 15 7

Additional Options:

Transmitted Light Detector-Kits
100 TLD for DM 101 TLD for DMI DM Basic 3-Plate Stage motorized kit 202 DM Basic 3-Plate Stage motorized kit upgr. 201 DMI Basic 3-Plate Stage motorized kit 203 DMI Basic 3-Plate Stage motorized kit upgr. 9b 9a 9b 410 Stage Insert for SuperZ, rotatable 411 Stage Insert for SuperZ, universal 412 Stage Insert for SuperZ, basic (system included)

Motorized xy-Stage Kits

SuperZ Galvo Stage Inserts
Optical Outfit DIC for Microscopes
Optical outfit DIC for DM 2500 CSQ 551 Optical outfit DIC for DM 5500 CSQ 451 Optical outfit DIC for DMI 4000 CSQ

Digital Camera Kit

8 9b 156605303 Leica DFC360 FX Digital Camera Kit SPE 304 Leica DFC310 FX Digital Camera Kit SPE 305 Roper CoolSNAP HQ2 Camera Kit SPE 306 Hamamatsu 9100-02 Camera Kit SPE
DIC Prism Sets for Basic Objective Sets
DM2500 Prism Set Basic DM Prism Set Basic DMI Prism Set Basic

Plate Inserts

420 Plate Insert -Microtiter Plate 421 Plate Insert -Willco Dish 35 mm 422 Plate Insert -Willco Dish 50 mm 423 Plate Insert -MatTek Dish 35 mm 424 Plate Insert -Petri dishes 36 mm 425 Plate Insert -Petri dishes 39 mm
DM2500 Prism Set Basic V-VIS DM Prism Set Basic V-VIS DMI Prism Set Basic V-VIS

Seite 7

Confocal Systems RGB/RYB
Accessories: pment External light source HXP120 burner
Leica TCS SPE w. DM2500 RGB Leica TCS SPE w. DM5500 RGB Leica TCS SPE w. DMI4000 RGB Leica TCS SPE w. DM2500 RYB Leica TCS SPE w. DM5500 RYB Leica TCS SPE w. DMI4000 RYB
HC-Objectives for RGB/RGBV Systems
Basic Objective Set HC 10x,40x,HC PL APO 10x/0.40 CS 15506326 HC PL APO 20x/0.70 Imm/Corr CS 15506251 HCX PL APO 40x/1.25 Oil CS 15506188 HCX PL APO 63x/1.40 Oil CS 15506279 HCX PL APO 63x/1.20 W Corr CS 15506194 HCX PL APO 63x/1.30 Glyc Corr CS 21
Confocal Systems RGBV/RYBV
Liquid light guide Adapter, Filter, Oculars Leica TCS SPE w. DM2500 RGBV Leica TCS SPE w. DM5500 RGBV Leica TCS SPE w. DMI4000 RGBV Leica TCS SPE w. DM2500 RYBV Leica TCS SPE w. DM5500 RYBV Leica TCS SPE w. DMI4000 RYBV
Confocal Prepared Microscope for all types
including Microscope Accessories
Leica TCS SPE DM2500 Confocal Prep. Leica TCS SPE DM5500 Confocal Prep. Leica TCS SPE DMI4000 Confocal Prep.
Leica TCS SPE Scanning Unit RGB Leica TCS SPE Scanning Unit RYB Leica TCS SPE Scanning Unit RGBV Leica TCS SPE Scanning Unit RYBV
ACS Objectives for RGBV/RYBV Systems
155935210 Basic ACS Objective Set 10x,40x,63x 15507902 ACS APO 10x/0.30 Dry CS 15507904 ACS APO 20x/0,60 Imm CS

IS o BF fit

15507901 ACS APO 40x/1.15 Oil CS 15507900 ACS APO 63x/1.30 Oil CS

Climate Control

602 Universal Gas Cover for SuperZ w. In/Outlet 9b 9c

Hardware-Options

Control Panel for TCS SPE 702 Second 19"-TFT Monitor for TCS SPE 703 Eizo 30" high brilliance monitor TCS SPE
Software LAS AF - Screening TCS SPE HCS A High Content Screening Automation
14 LEICA HCS A for TCS SPE-II see LAS AF Matrix Products
Software LAS AF - Imaging TCS SPE
202 LAS AF SPE Live Data Mode 203 LAS AF SPE Co-Localisation
600 TCS SPE CO2 Control Set 110V/230V 9c Including: SuperZ CO2 Cover CO2 Controller, Humidifier

Tables

Microscope Table for Upright and Inverted Systems, passive 190 Computer Table
204 LAS AF SPE Deconvolution 205 LAS AF SPE 3D Visualisation 208 LAS AF SPE Dye Finder 211 LAS AF SPE Camera Advanced 215 LAS AF SPE FRAP
400 Climate Chamber for DMI 4000 CSQ Including Heating & Temp Control Unit 10 12

Seite 8

Room Requirements
Power Supply Power supply integration Type Voltage range Power consumption Independent circuits Frequency Fuse: standard Humidity (noncondensing) Operating temperature Guaranteed stability Confocal unit, max Microscope, max System Static floor load [V] [VA] [no.] [Hz] [A] [%] [C] yes autoselect 100 - 1 50/10 - - 30 23C +/- 2C 120 150

visible radiation:

Load Capacity and Weight

[kg] [kg] [kg] [kg/m2]

www.leica-microsystems.com
LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.