Biophysical Journal (ISSN 0006-3495) is published monthly (two volumes of six issues each) by the Biophysical Society, 9650 Rockville Pike, Room L-0502, Bethesda, Maryland 20814-3998 for delivery in the USA at $810 and for all other countries at $860 per calendar year. Canadian GST no. 898477062. Periodicals postage paid at Bethesda, MD and additional mailing offices. Postmaster: Send address changes to Biophysical Joumal. Order Service, Biophysical Society Office, 9650 Rockville Pike, Bethesda, MD 20814-3998. Copyright 2000 by the Biophysical Society. Printed in the United States of America. Subscriptions to Biophysical Journal All members of the Biophysical Society receive BJ Online as a benefit of membership. Members may purchase print journal subscriptions for $60 (US members) $120 (foreign members). Non-member subscriptions may be ordered through: Subscription Manager Biophysical Society 9650 Rockville Pike, Room L-0502 Bethesda, MD 20814 society@biophysics.faseb.org

Publications Committee

Richard Horn Jack H. Kaplan Michael L. Klein Peter Kollman Barry R. Lentz Leslie M. Loew Gary Matthews Stuart G. McLaughlin Edward G. Moczydlowski Richard L. Moss Colin G. Nichols B. Montgomery Pettitt M. Thomas Record, Jr. Lynne J. Regan Emil Reisler Dagmar Ringe Benoit Roux Michael Sanguinetti John R. Silvius Jeffrey Skolnick John L. Spudich Marion Thurnauer Douglas H. Turner Anthony Watts Sunney Xie William N. Zagotta
Clare K. Woodward, Chair Yale E. Goldman Timothy A. Keiderling Nadrian C. Seeman Eugene Stanley Brian Sykes

Editorial Office

Dianne McGavin, Publications Manager Rosemary Sachs, Publications Assistant Christina Williams, Production Coordinator Julia Petteway, Technical Projects Coordinator Jeanne Brooksbank, Marketing & Sales Manager Drita Schruefer, Information Systems Administrator
Copy Editing & Production

Leilani Cochran

Claims for nonreceipt of issues will be honored when received within 3 months of mailing for US subscribers, 6 months for foreign subscribers. For a single issue the price is $65.00 in the United States and $70.00 outside the United States, payable in advance. Remittances should be made by draft or check drawn on US banks or by postal money order, payable to Biophysical Joumal. Inquiries about backfiles of the Joumal in microfilm or microfiche should be addressed to University Microfilms, 300 North Zeeb Road, Ann Arbor, Ml 48106. Back volumes 1-13 (1960-1973) are handled by AMS PRESS Inc., 56 East 13th Street, New York 10003; write directly to AMS for information about these issues. For information about other issues contact the Biophysical Society Office. Submission of Manuscripts Submit manuscripts to: Biophysical Journal Peter B. Moore, Editor 9650 Rockville Pike Bethesda, MD 20814

Coffee Break

Session IV Drug Discovery for Ion Channels
3:45-4:30 PM Neil Castle, ICAgen From Genomics to Discovery of Ion Channel Drugs 4:30-5:15 PM Wilhelm G. Lachnit, Molecular Devices HTS and Drug Screening for Ion Channels 5:15-6:30 PM Thomas M. Connolly Merck Research Labs Ion Channel Screening

6:30-8:30 PM

Session II Gene Discovery of Ion Channels

10:45-11:30 AM

Rory Curtis
Millennium Pharmaceuticals, Inc. Genomic Discovery of Ion Channels Past, Present and Future

Cocktail Reception

2:00 PM
All rooms in Convention Center unless otherwise noted.
Presentation of Young Investigator Awards for 2001

BIOENERGETICS SUBGROUP

8:30 AM-7:00 PM, Room 312 (Generously supported by Avanti Polar Lipids, Bristol-Myers Squibb, MitoKor, and Bio-Rad Laboratories, Life Science Group.)
Afternoon Platform Session: Protein Import into Mitochondria
Kathleen W Kinnally, Organizer New York University College of Dentistry

2:05 PM

Young Investigator Awardee Poster Session
Morning Platform Session: Mitochondria and Disease
Hartmut Wohlrab and Maureen McEnery, Organizers

8:50 AM

Introduction Kathleen W Kinnally New York University College of Dentistry

2:15 PM

Introduction
Hartmut Wohlrab Boston Biomedical Research Institute and
Bioenergetics of Mitochondrial Protein Translocation Walter Neupert University of Munich

3:00 PM

Harvard Medical School

9:00 AM

Mitochondrial Mutation in Cancer Progression and

Detection

Translocation Across and Insertion into the Mitochondrial Inner Membrane Robert Jensen Johns Hopkins University School of Medicine
Makiko S Fliss Johns Hopkins University School of Medicine 9:45 AM Free Radical Mediated Dedines in Mitochondrial Function during Cardiac Ischemia/Reperfusion Luke L Szweda Case Western Reserve University School of Medicine

3:45-4:00 PM

Coffee break
4:00 PM The Protein Translocation Channels of Mitochondria Kathleen Kinnally New York University College of Dentistry

PLATFORMS SESSIONS E-H

11:00 AM-1:00 PM, ROOMS 302, 304, 311, & 312
E- Self Assembled Session: Physics & Structural Biology of Monolayers, Bilayers & Proteins: Phys & Phenom & Methods, Room 311 F- Self Assembled Session: Structure & Gating in Cation Channels I, Room 302 G-Transporters & Exchangers, Room 312 H-Rhodopsin & Visual Receptors, Room 304
Non-L-Type Voltage-gated Ca2+ Channels (521-554) Intracellular Communication (555-562) Gap-Junction Channels (563-573) Bacterial Channels (574-580) Peptide & Toxin Ion Channels (581-613) Endoplasmic Reticulum & Protein Trafficking (614-626) Exocytosis & Endocytosis (627.1-627.14) Polymer Physical Chemistry (628-632) Biotechnology & Bioengineering (633-654) Calorimetry (655) Single Molecule Biophysics (655.01-655.53) Imaging & Optical Microscopy (656.01-656.57) Theory I (656.58) SRAA Posters

SYMPosIuM 5

CPOW PANEL DISCUSSION
12:00 NOON-2:00 PM, ROOM 202

3:45-5:45 PM, AUDITORIUM

Sizing up Single Molecules
Sunney Xie, Chair, Harvard University 3:45 PM Single Molecule Studies of Enzyme Activity and Folding Steven Chu, Stanford University 4:15 PM Studying Replication and Transcription One Molecule at a Time Carlos Bustamante, HHMI University of California, Berkeley 4:45 PM Imaging and Spectroscopy of Single Molecules with Carbon Nanotube Probes Charles Lieber, Harvard University 5:15 PM Single-Molecule Enzymatic and Conformational Dynamics Sunney Xie, Harvard University
Navigating the Transition from Postdoctoral to Independent Scientist: A Discussion with Representatives from Industry and Academia
The discussion will cover topics including choosing between industry and academia, how to approach the application process, negotiating a new position, and expectations of the newly independent scientist. All meeting participants are invited to attend.

POSTER SESSIONS

1:00-3:45 PM, HALLS C & D
(Numbers in parentheses refer to abstract numbers, found beginning on page IA.)
Ion Motive ATPases (145-170) Na/Ca and Na/H Exchangers (171-182) Electron Transfer Systems (183-201) Erythrocyte Membranes (202-204) Membrane Proteins (205-243) G Proteins (244-252) Protein Structure (253-280) Calcium Sparks (281-301) Signal Transduction in Muscle Cells (302-314) Muscle & Motility, Other (315-333) Actomyosin Interactions (334-367) Muscle Regulatory Proteins (368-419) Actin & Actin-binding Proteins (420-443) Microtubules & Microtubules-binding Proteins (444-449) Neuronal Systems & Modeling (450-457) Ligand-gated Channels (458-489) Mechanosensitive Channels (490-504) Biophysics of Ion Permeation (505-520)

PLATFORM SESSION I-M

3:45-5:45 PM, BALLROOM C ROOMS 302, 304, 311 & 312
I - Na Channels, Room, Ballroom C J - Self Assembled Session: Physics & Structural Biology of Monolayers, Bilayers, & Proteins: Biological Relevance, Room 311 K-Cardiac Muscle, Room 302 L - Protein Structure, Liganding & Mechanism, Room 312 M-Photosynthesis (Plant & Bacteria), Room 304

SRAA COMPETITION

6:00 PM, ExHIBIT HALLS C & D

WORKSHOPS

7:30-10:30 PM, BALLROOMS A-C

Workshop I: BALLROOM A

Putting the Pressure on Proteins
Catherine Royer, Centre de Biochimie Structurale, Montpellier Jerson Silva, Universidade Federal do Rio de Janeiro Co-chairs 7:30 PM Introduction 7:35 PM Modeling Pressure Effects in Protein Folding Kinetics and Thermodynamics Angel Garcia, Los Alamos National Laboratory 8:10 PM Pressure-Jump Kinetics of Protein Folding: Volume and Hydration Changes in Folding/Unfolding Reactions Catherine Royer, Centre de Biochimie Structurale, Montpellier 8:45 PM Protein Folding Misfolding and Amyloidogenesis as Studied by High Pressure Jerson Silva, Universidade Federal do Rio de Janeiro 9:20 PM Pressure as a Tool for Protein Dissection Andrew joshua Wand, University of Pennsylvania 9:55 PM Use of Pressure for Studying the Energetics and Kinetics of Biomolecular Phase Transformations: Proteins in Solution and Lipid Bilayers Roland Winter, University of Dortmund

WORKSHOP II: BALLROOM C

8:30 PM Flippases, Floppases, and Scramblases: Regulators of Transbilayer Lipid Organization Dave Daleke, Indiana University 9:00 PM Lipid Rafts in Model & Biological Membranes Ken Jacobson, University of North Carolina 9:30 PM Sphingolipid and Cholesterol-Rich Membrane Microdomains Deborah Broum, SUNY Stony Brook 10:00 PM

Open Discussion

WORKSHOP III: BALLROOM B
Databases for Biophysicists
Martin Caffiry, Chair, Ohio State University 7:30 PM The Lipid Data Bank Martin Caffiey, Ohio State University 7:55 PM LIPIDAT and LIPIDAG Databases and Their Uses Stephanie Tristram-Nagle, Carnegie Mellon University 8:15 PM The Protein Data Bank and the Nucleic Acid Databases Helen Berman, Rutgers University 8:40 PM The CATH Domain Database: A Structural Perspective on Protein Evolution Christine Orengo, University College, London 9:05 PM NCBI Databases Steve Bryant National Center for Biotechnology Information 9:30 PM The Protein Information Resource (PIR) Database and Tools Winona Barker National Biomedical Research Foundation 9:55 PM The Binding Database Michael K Gilson University of Maryland Biotechnology Institute 10:15 PM Electrophysiology Databases: A Common Data Model Daniel Gardner, Cornell University Medical College

Dan Oprian, Chair, Brandeis University
11:00 AM Binding at Signaling Interfaces James Wels, Sunesis Pharmaceuticals, Inc.

11:30 AM

High-Resolution Structural Studies of Receptors Wayne Hendrickson, Columbia University

12:00 PM

W-Electron Transfer Systems, Oxidative Phosphorylation, Room 304

EDUCATION ROUNDTABLE

12:00 NOON-2:00 PM, ROOM 202 Bernard Chasan, Boston University, and Barry Selinsky, Villanova University, Co-Chairs An open roundtable discussion, sponsored by the Education Committee.
Activation of G Protein-Coupled Receptors Dan Oprian, Brandeis University 12:30 PM Transmembrane Signaling and Adaptation in Bacterial Chemoreceptors Gerald Hazelbauer, University of Missouri, Columbia

SYMPOSIUM 9

1 1:00 AM-1:00 PM, BALLROOM B
1:00-3:45 PM, HALLS C & D (Numbers in parentheses refer to abstract numbers, found beginning on page 1A.) Channel Regulation & Modulation (792-836) Voltage K Channels I (837-891) Voltage-gated Na Channels (892-939) Presynaptic Channels & Release Mechanisms (940-945) Mitochondrial Channels (946-968) Ion Channels, Other (969-997) Membrane Receptors & Signal Transduction (998-1034) Membrane Transporters & Exchangers (1035-1052) Cardiac Muscle I (1053-1079) Muscle Mechanisms & Ultrastructure (1080-1130) Cytoskeleton (1131-1143) Enzymes (1144-1163) Heme Proteins (1164-1203) Virus Structure & Assembly (1204-1215) DNA Binding Proteins (1216-1224) X-ray Diffraction (1225-1228) Solution NMR (1229-1237) EPR Spectroscopy (1238-1244) Vibrational Spectroscopy (1245-1256) Atomic Force Microscopy(1257-1279) Theory II (1280-1281) Protein Ligand Interaction I: Thermodynamics & Kinetics (1282-1296) Protein Ligand Interactions II: Spectroscopic & Other Physical Studies (1297-1319) Protein-Ligand Interactions III: Protein Engineering Approaches (1320-1326) Protein Ligand Interactions IV: Computational Studies (1327-1336) Protein Structure Prediction (1337-1342) Structure-based Drug Design (1343) Computational Methods (1344-1361) Molecular Dynamics (1362-1376)

PLATFORM SESSION AG-AK

8:30-10:30 AM BALLROOMS A & C, RooMs 304, 311 & 312
Ligand Recognition by Olfactory Receptor Neurons Randall R. Reed, Johns Hopkins University School of Medicine/HHMI 11:30 AM Coding Odor Information: From Biology to an Artificial Olfactory System John S. Kauer, Tufts University
12:00 PM 02 Sensors from Bacteria and Archaea Maria-Alda Gilles-Gonzalez, Ohio State University 12:30 PM Crystal Structure of CooA: A CO-Sensing Transcription Factor Thomas Poulos, University of California, Irvine
AG-E-C Coupling & Calcium Sparks I, Ballroom A AH-Phospholipid Membrane Structure, Ballroom C AI -Protein-Ligand Interactions, Room 311 AJ -Gating Channels by External & Internal Ligands,
Room 312 AK-New Biophysical Methods, Room 304

PLATFORM SESSIONS AL-AP

11:00 AM-1:00 PM BALLROOM A & C, ROOMS 304, 311 & 312
AL- Protein Conformation, Dynamics & Assemblies II, Ballroom A AM-Motility & Regulatory Proteins, Ballroom C AN-Receptors & Signal Transduction, Intracellular Communication, Room 311 AO-Mechanosensitive & Acid-Sensing Channels, Room 312 AP -Exocytosis, Endocytosis & Trafficking, Room 304
STUDENT EDUCATION PROGRAM
11:00 AM-2:00 PM, ROOM 302 Richard Ludescher, Organizer, Rutgers University Educational program for high school and undergraduate college students and their faculty will include the Emily M. Gray Award Lecture and presentations by speakers on topics of general interest in biophysics, a poster session describing research, and educational projects undertaken by the students. All Society members are encouraged to attend.
What is Biophysics Richard Ludescher, Rutgers University
Cryo Electron Microscopy & Reconstruction (1556-1565) Biophysical Chemistry, Other (1566-1580) Excitation-Contraction Coupling & Skeletal Muscle (1581-1601) Ryanodine Receptors (1602-1629) Smooth Muscle (1630-1665) Protein Conformation & Dynamics (1666-1733) Protein Assemblies & Aggregates (1734-1756) Molecular Chaperones (1757-1761) Membrane Fusion (1762-1786) Lipid Physical Chemistry (1787-1814) Bacterial Photosynthesis (1814.5-1817) Plant Photosynthesis (1818-1828) Oxidative Phosphorylation (1829-1835) Membrane Transport (1836-1845) Voltage-gated K Channels II (1846-1905) Modulation of Voltage-gated Ca2+ Channels (1906-1928) Molecular Mechanism of Ca2+ Signaling (1929-1953) Intracellular Channels (1954-1961) Acetylcholine Receptors (1962-1981) Anion Channels (1982-2011) Epithelial Channels & Physiology (2012-2024) Replication & Recombination (2025-2031) DNA Repair(2032-2036) Transcription (2037-2045) Translation (2046-2047) DNA, RNA Structure & Conformation (2048-2075) DNA-Drug Interactions (2076-2086) Physical Chemistry of Nucleic Acids (2087-2093) Nucleic Acids, Other (2094-2105) High School Student Posters

Role of Sialate Bound Influenza Hemagglutinin During Fusion Aditya Mittal (1774-Pos, Board 270)
Interaction Between SIV gp4l and Membranes: A Novel Role for gp4l Ectodomain in Membrane Fusion Sergio Peisajovich (1775-Pos, Board 271)
Correlation of A,B Aggregation State to Changes in Bilayer Structure in Liposomes John Kremer (2446-Pos, Board 133)
Where Do Amyloid Peptides Lie on Lipid Membranes? Canay Ege (247 1-Pos, Board 158)
Negative Feedback Function of Kal Channels in Cerebral Arterioles
Fat-Chong (Alex-Pos,) Cheong-Chun-Lin (1803-Pos, Board 299)
Mutational Changes in the Putative "Antiporter Motif" of the Multifunctional Tet(L-Pos,) and Tet(K-Pos,) TetracyclineMetal/H+ Antiporters Alter the Ion Coupling Properties Jie Jin (1845-Pos, Board 342)
Nanosecond Dynamics of Peptide Folding and Unfolding by Temperature Jump Infrared Probe Spectroscopy Shelia Maness (2486-Pos, Board 173)
Modification of an Extracellular Residue by Mtset Mimics the Effect of Acidosis on the Kv1.4 K+ Channel Thomas Claydon (1887-Pos, Board 384)
Probing MCAK's Microtubule Depolymerizing Activity in Vitro Andrew Hunter (2578-Pos, Board 265)
Recovery of Cardiac Ca Signaling from Ca Release-Induced
Inactivation Zuzana Kubalovd (2676-Pos, Board 363)
Gene Expression of SERCA2a, L-and T-Type Ca Channels During Human Heart Development Yangxia Qu (271 1-Pos, Board 398)
Modulation of Cardiac K+ Currents by 17[B-Estradiol in Mouse Ventride Veronique Trlpanier-Boulay (1889-Pos, Board 386)
Modification of K Channel Gating by a Mercaptoindole Jon Sack (1898-Pos, Board 395)
Reducing External pH Decreases External Lock-In and Slows Activation Gating for Low-Voltage-Activated Ca Channels Brian Delisle (1914-Pos, Board 41 1)
Conformational Changes of Bacteriorhodopsin LynellMartinez (2722-Pos, Board 409)
Role of Ca2+ and Voltage-Dependent Inactivation in Cavl.2 Inhibition by (-) Gallopamil Stanislav Sokolov (2798-Pos, Board 485)
A Glutamate Residue GIRK4-E146Q in the H5 Pore Region of GIRK1/GIRK4 Affects Channel Characteristics Katharine Dibb (2816-Pos, Board 503)
Differential IP3 Receptor Mediated Calcium Signaling in Zebrafish Purkinje Cells of the Cerebellum Friedrich WJohenning (1934-Pos, Board 431)
Binding Kinetics of Calbindin-D28k Determined by Flash Photolysis of Caged Ca2+ Valentin Naegerl (1951-Pos, Board 448)
CFTR Functions as a Monomer Chen Jey-Hsin (2013-Pos, Board 510)
Conformational Changes in the Pore of Rod Cyclic NucleotideGated Channels Revealed by State-Dependent Block Kimberly Craven (2849-Pos, Board 536)
Disulfide Bond Formation in the Ligand-Binding Domain of Rod Cyclic Kimberly Matulef(2852-Pos, Board 539)

Basl, A., 574-Pos, 575-Pos Bass, A.H., 1084-Pos Bassey, C., 646-Pos Basso, C., 1483-Plat, 1853-Pos Batenjany, M.M., 2324-Pos Bates, F.S., 1037-Pos Ba-Thein, W., 147-Pos Batiza, A.F., 1449-Plat Baud, F., 1720-Pos Bauer, E., 989-Pos Bauer, P.J., 86-Plat Bauer, R, 2352-Pos Baumann, C.G., 2042-Pos, 2571-Pos Baumann, W.J., 1388-Plat Baumgarten, C.M., 2867-Pos Baumgartner, W., 2118-Plat Baur, A., 654-Pos Bausch, A., 2117-Plat Bausch, A.R, 1135-Pos Bausman, J., 2885-Pos Bax, A., 698-Plat Baxter, D.F., 634-Pos Bayer, J., 1273-Pos Bayerl, T.M., 2380-Pos, 2381-Pos Bayguinov, O.P., 1944-Pos Bayley, H., 601-Pos, 602-Pos, 2114-Plat Bayley, P.M., 1134-Pos, 1316-Pos Baylor, D., 80-Plat Baylor, S., 294-Pos Baylor, S.M., 295-Pos, 1121-Pos Bayramov, S.K., 1160-Pos Baysal, C., 1696-Pos Bazzini, C., 2009-Pos Beach, B.M., 256-Pos Beall, B.M., 435-Pos Beam, K.G., 1381-Plat, 2793-Pos Beattie, D.S., 187-Pos Beatty, A.L., 1565-Pos Beatty, J.T, 1815-Pos Beaumont, J., 2908-Pos Beaurepaire, E., 656.05-Pos Beavis, A.J., 639-Pos Bechhofer, D.H., 1845-Pos Beck, E.J., 1868-Pos Beck, R., 848-Pos Becker, A., 1040-Pos Becker, M., 268-Pos Beckett, D., 682-Plat, 1403-Plat Beech, DJ., 237-Pos, 973-Pos, 1903-Pos Beechem, J.M., 656.42-Pos Beedle, A.M., 542-Pos Beerman, J., 1847-Pos Beeton, C., 1846-Pos Befus, D.A., 1694-Pos Begin, K., 377-Pos, 1061-Pos Behnke, C.A., 1465-Symp
Behrends, J.C., 622-Pos, 1413-Plat Beier, N., 385-Pos Beker, F.E., 1980-Pos Belagaje, R., 1018-Pos Belagyi, J., 326-Pos Belenky, M., 85-Plat Belevych, A.E., 2868-Pos Belknap, B., 353-Pos, 354-Pos Bell, J.D., 203-Pos, 2474-Pos Bell, S.P., 1070-Pos Bellamy, H., 697-Plat Bellet-Amalric, E., 60-Plat Bell-Homer, C.L., 477-Pos Bellisent-Funel, M.C., 2379-Pos Belmont, A., 656.56-Pos Belomoin, G., 1524-Pos, 1525-Pos Belterman, C., 2743-Pos Belus, A., 2874-Pos Benakezouh, Z., 2797-Pos Benavides-Haro, D., 2877-Pos Ben-Avraham, D., 1140-Pos Bendahhou, S., 750-Plat, 919-Pos, 938-Pos Bender, K., 2817-Pos Benedek, G.B., 1709-Pos, 1728-Pos, 2152-Plat Benedetti, A., 2765-Pos Benevides, J.M., 1212-Pos, 1215-Pos, 1676-Pos, 2061-Pos Benitah, J., 803-Pos Benkusky, N.A., 834-Pos Benndorf, K., 656.34-Pos, 892-Pos Benndorf, K., 2888-Pos Bennett, E.S., 903-Pos Bennett, J.S., 1477-Plat Bennett, M.V., 48.4-Plat, 571-Pos Bennett, PB., 845-Pos Benson, C.J., 2164-Plat Benson, L.M., 271-Pos Ben-Tabou, S., 767-Plat Ben-Tal, N., 221-Pos Bentz, J., 1770-Pos, 1774-Pos Benveniste, M., 474-Pos BenzaniLla, F., 862-Pos Beratan, D.N., 186-Pos, 2142-Plat Bdraud, E., 1846-Pos Beretta, S., 655.08-Pos, 2382-Pos, 2384-Pos Berg, H.C., 315-Pos, 773-Plat Berger, A.L., 2001-Pos Berger, C., 775-Plat Berger, C.E., 779-Plat Berger, M.D., 2030-Pos Bergsma-Schutter, W., 1780-Pos Berg-Sorensen, K., 655.3-Pos, 656.22-Pos

Graneli, A., 105 1-Pos Graner, F., 60-Plat, 656.49-Pos Grant, C.W., 2393-Pos Grant, D.F., 2896-Pos Granzier, H., 1069-Pos, 1070-Pos, 1071-Pos Granzier, H.L., 1072-Pos, 2119-Plat Grassi, C., 1920-Pos Grassucci, R., 2555-Pos Gratton, E., 133-Wkshp, 656.06-Pos, 656.1-Pos, 656.14-Pos, 656.31-Pos, 656.56-Pos, 1508-Pos, 1514-Pos, 1524-Pos, 1525-Pos, 1536-Pos, 2376-Pos, 2382-Pos, 2384-Pos, 2463-Pos, 2509-Pos, 2570-Pos Gratton, E.C., 656.39-Pos Graves, D.E., 2081-Pos Gray, D.M., 1222-Pos Gray, P., 496-Pos Greaser, M.L., 2608-Pos Greathouse, D.V., 587-Pos, 589-Pos, 609-Pos, 2397-Pos, 2438-Pos, 2439-Pos Green, M.E., 837-Pos Green, R., 2107-Symp Greenbaum, N.L., 2063-Pos Greene, A.E., 735-Plat Greenfield, N., 394-Pos Greenfield, N.J., 227-Pos, 2522-Pos Greenstein, J.L., 1361-Pos, 268 1-Pos Greenwood, IA., 1631-Pos Greer, S.C., 426-Pos Gregg, R.G., 546-Pos Grego, S., 444-Pos, 655.3-Pos Gregor, W, 1241-Pos Gregory, S.T., 2555-Pos Gregurick, S.K., 1256-Pos Greilberger, J., 2902-Pos Grewer, C.T., 71-Plat Gribbon, P., 635-Pos Griesinger, C.B., 656.03-Pos Griffin, M., 291 1-Pos, 2914-Pos Griffin, R.G., 85-Plat, 1545-Pos, 1546-Pos, 2728-Pos Griffith, L.G., 638-Pos Griffiths, P., 1 100-Pos Grigera, J.R, 1710-Pos Grigorieff, N., 1421-Symp, 1563-Pos Griko, YV., 611-Pos Grimard, V., 1687-Pos Grimes, S., 786-Plat Grimshaw, S.B., 2503-Pos Grishin, S.N., 942-Pos Grissmer, S., 458-Pos, 463-Pos, 974-Pos
Grobaski, T.D., 1893-Pos Grobner, G., 1547-Pos, 2417-Pos Grone, D.L., 656.09-Pos Groome, J.R, 936-Pos Groschner, K., 795-Pos, 805-Pos, 896-Pos, 1917-Pos, 2794-Pos, 2795-Pos Grosman, C., 1977-Pos, 1978-Pos Gross, C.H., 14-Subg Gross, D.J., 1443-Plat Gross, E.L., 1827-Pos, 1828-Pos Gross, J.D., 51-Symp Grossfield, A., 1793-Pos Grossman, A.D., 2047-Pos Groth, G., 212-Pos Groves, J.T., 643-Pos, 1022-Pos, 1180-Pos
Gurel, O., 1695-Pos Gurevich, V.V., 1682-Pos Gurnev, PA., 596-Pos, 599-Pos Gurney, A.M., 1859-Pos Gurrola, G.B., 2886-Pos Gursky, O., 262-Pos, 274-Pos, 758-Plat Gusev, K., 2788-Pos Gusev, N.B., 380-Pos Gushchin, M.I., 2614-Pos Gustin, M.C., 1451-Plat Gutberlet, T., 240 1-Pos Guthold, M., 1274-Pos Gutierrez, A.A., 2764-Pos Gutierrez, A.M., 2020-Pos Gutierrez-Cirlos, E.B., 195-Pos Guttenberg, Z., 1006-Pos Guy, H., 495-Pos, 496-Pos Guy, H.R., 1450-Plat,

Sternin, E., 2358-Pos, 2419-Pos Sternweis, PM., 249-Pos Steven, A., 1742-Pos Steven, A.C., 755-Plat Steven, L.J., 1384-Plat Stevens, C.F., 1004-Pos Stevens, M.J., 2075-Pos Stevenson, L.M., 1700-Pos Stewart, A., 1085-Pos, 1090-Pos Stewart, J., 2423-Pos Stewart, R.J., 2197-Plat Sticht, H., 2540-Pos Stieglitz, K.A., 714-Plat Stillman, J., 2527-Pos Stillwell, W., 2325-Pos, 2326-Pos, 2331-Pos, 2338-Pos, 2349-Pos Stimac, R., 157-Pos Stimers, J.R., 2896-Pos
Stites, WE., 15 10-Pos, 2544-Pos Stitt, M., 656.32-Pos Stobiecka, A., 2021-Pos Stock, A.M., 658-Symp Stock, J., 769-Plat Stockell, D., 115-Plat Stoicheva, N.G., 2391-Pos Stojiljkovic, L., 2598-Pos Stojilkovic, S.S., 1016-Pos Stokes, A., 2749-Pos Stone, D.A., 1824-Pos Stone, M.P., 2084-Pos Storm, A., 2064-Pos Stotz, S.C., 522-Pos Stout, A.L., 655.34-Pos Stowe, D.E, 181-Pos, 1469-Plat, 2625-Pos, 2742-Pos, 2744-Pos Straight, A., 1 121-Pos Strain, C., 1657-Pos Strambini, G.B., 1050-Pos Strand, J., 409-Pos Strand, K., 112-Plat, 1693-Pos Strandberg, E., 2441-Pos Strasburg, G., 1599-Pos Strasburg, G.M., 1627-Pos Straub, J.E., 1685-Pos, 2514-Pos Straub, S.V., 1945-Pos Strauss, C.E., 2482-Pos Strauss, H.C., 796-Pos, 1873-Pos Streaker, E.D., 1403-Plat Strekowski, L., 2405-Pos Strey, H.H., 263-Pos, 2070-Pos Striessnig, J., 545-Pos, 1915-Pos, 1916-Pos Strobel, S., 2106-Symp Stroffekova, K., 2793-Pos Stroppolo, M., 664-Plat Stroud, P., 1705-Pos Stroud, R.M., 996-Pos Strube, C., 546-Pos Strubing, C., 48.7-Plat Struth, B., 2371-Pos Strzalka, J., 34-Plat, 220-Pos, 649-Pos Stuehr, D.J., 1199-Pos, 1376.1-Symp Stull, J., 1497-Plat Sturek, M.S., 1058-Pos Su, C., 1241-Pos Su, J.Y., 302-Pos, 303-Pos Su, M., 1232-Pos Su, Y., 2493-Pos Suadicani, S.O., 1034-Pos Subramaniam, S., 1557-Pos, 1559-Pos, 1565-Pos, 1677-Pos Subramaniam, T., 476-Pos Subramaniam, V., 33-Plat Suchyna, T., 499-Pos
Suda, H., 2593-Pos Sue, S., 1303-Pos Suel, G.M., 1004-Pos Sugar, I.T., 2362-Pos., 2363-Pos Suggs, J.A., 2183-Plat, 2590-Pos Sugi, H., 1124-Pos Sugiura, S., 1124-Pos Sugiura, Y, 1480-Plat Suh, Y., 2504-Pos Suhkorukov, G., 1794-Pos Sukal, S., 1307-Pos Sukharev, S., 495-Pos, 496-Pos, 1450-Plat Sukhareva, M., 1929-Pos Sukow, C., 2120-Plat Sullivan, D., 1970-Pos Sullivan, J., 454-Pos Sullivan, J.M., 79-Plat Sullivan, M., 727-Plat Sullivan, SA., 1893-Pos Sumandea, M.P., 386-Pos Summers, M.F., 269-Pos Sumners, C., 548-Pos Sun, H., 550-Pos Sun, H., 589-Pos Sun, H., 1949-Pos Sun, H., 2397-Pos Sun, J., 817-Pos Sun, J., 1363-Pos Sun, Y., 392-Pos Sun, Y., 655.39-Pos Sun, Y, 218 1-Plat Sunami, A., 908-Pos, 924-Pos Sundaralingam, M., 2048-Pos Sundaram, S., 589-Pos, 521-Pos, 524-Pos, 525-Pos, 526-Pos Sung, R., 2899-Pos Sung, U., 971-Pos Superfine, R., 1206-Pos Suppiramaniam, V., 476-Pos Surek, J., 1242-Pos Surprenant, A., 473-Pos,
1459-Plat Susa, I., 2741-Pos Sussman, F.S., 1335-Pos Sussman, J.L., 2128-Plat Sutherland, G.R., 486-Pos Sutherland, M., 2620-Pos Sutin, J., 425-Pos, 656.25-Pos Sutin, J.D., 656. 1-Pos, 2570-Pos Sutin, J.J., 656.36-Pos Sutka, M.R., 1044-Pos Sutko,J., 1956-Pos Sutoh, K., 339-Pos, 656.1 1-Pos Sutton, K.G., 534-Pos Suzuki, K., 1023-Pos Suzuki, M., 336-Pos Suzuki, T., 363-Pos Suzuki, Y., 656.1 1-Pos

Wingerd, K.L., 2842-Pos Wingfield, P., 1742-Pos Winkler, H., 1087-Pos Winslow, R.L., 1361-Pos, 2681-Pos Winstone, T.L., 224-Pos Winter, R.H., 131-Wkshp Winterhalter, M., 576-Pos, 640-Pos, 1426-Plat Winters-Hilt, S., 785-Plat, 213 1-Plat Wirtz, K.W, 1032-Pos WWischmeyer, E., 976-Pos Wise, J.G., 117-Plat Wiseman, P.W, 2161-Plat Wisner, M.A., 2407-Pos Wissler, J.H., 2556-Pos Wit, A.L., 566-Pos Witt, C.C., 1067-Pos Witt, S., 1759-Pos Witten, TA., 2344-Pos
Wittung-Stafshede, P., 2523-Pos, 2524-Pos Wnag, W., 1165-Pos Woehlke, G., 2575-Pos Wohlrab, H., 1046-Pos Wojcikiewicz, E., 655.46-Pos Wojtuszewski, K., 47-Plat,

2564-Pos

Wolanin, P., 769-Plat Woledge, R.C., 366-Pos Wolff, M.R., 2870-Pos Wolkers, WF., 1248-Pos Wolska, B.M., 1437-Plat Wolters, A., 1898-Pos Womack, M., 992-Pos, 993-Pos, 994-Pos Wonderlin, WF., 619-Pos Wong, C., 2658-Pos Wong, G.C., 436-Pos Wong, J., 59-Plat, 1393-Plat,

1398-Plat

Wong, J.Y, 324-Pos Wong, L., 1132-Pos Wong, T.C., 1355-Pos
Woo, S., 286-Pos Woo, S., 2172-Plat Woodard, S., 277-Pos Woodbury, D.J., 21 1-Pos,

211 1-Plat

Woodbury, J.W, 516-Pos
Woodsome, TP., 1655-Pos Woodward, J.T., 647-Pos Woody, R.W, 1203-Pos, 1222-Pos, 1342-Pos,
2497-Pos Woolf, P., 2354-Pos Woolf, T.B., 1328-Pos, 1375-Pos, 1723-Pos, 1793-Pos, 2141-Plat, 2348-Pos, 2464-Pos,

2720-Pos

Woolley, A., 591-Pos Woolley, GA., 2551-Pos Woosley, R., 855-Pos Wordeman, L., 704-Symp,

2578-Pos

Wotring, V.E., 506-Pos Wraight, C.A., 120-Plat,

1814.5-Pos

Wray, D., 2841-Pos Wriggers, W., 429-Pos, 1690-Pos, 1752-Pos Wright, V.P., 305-Pos, 1983-Pos
Wu, C.H., 142-Wkshp Wu, C.W., 2553-Pos Wu, D.T, 1360-Pos Wu, G., 2594-Pos Wu, H., 126-Plat Wu, H.Y, 1432-Plat Wu, J., 2363-Pos Wu, L., 1310-Pos Wu, M., 1648-Pos Wu, M., 1657-Pos Wu, M., 2086-Pos
Wu, W., 1303-Pos Wu, X., 1008-Pos Wu, Y, 731 -Plat Wu, Y., 762-Plat Wu, Y., 1069-Pos Wu, Y., 1070-Pos Wu, Y., 1384-Plat Wu, Y., 2603-Pos Wu, Y., 2604-Pos Wu, Y, 2883-Pos Wucherpfennig, K.W, 266-Pos Wulff, H., 978-Pos, 1846-Pos Wuskell, J.P., 656.16-Pos, 656.46-Pos Wussling, M.H., 2750-Pos, 2751-Pos Wymore, T., 1355-Pos, 2348-Pos
Xu, Q., 1315-Pos Xu, S., 237-Pos Xu, S., 1091-Pos Xu, S., 1092-Pos Xu, S., 1264-Pos Xu, X., 568-Pos Xu, X., 1 190-Pos Xu, X., 1389-Plat Xu, X., 2492-Pos Xu, X., 2883-Pos Xu, Y, 351-Pos Xue, H., 1689-Pos Xue, X., 2644-Pos Xun, L., 655.24-Pos
Xian, W., 443-Pos Xiang, T., 1047-Pos Xiang, T., 1367-Pos Xiao, G., 806-Pos Xiao, G., 1009-Pos Xiao, J., 2033-Pos Xiao, K., 197-Pos Xiao, K., 737-Plat Xiao, L., 217-Pos Xiao, M., 345-Pos Xiao, T., 759-Plat Xiao, Y., 468-Pos Xiao, Y., 902-Pos Xie, A., 2729-Pos Xie, L., 2833-Pos Xie, X, 655.24-Pos Xie, X.S., 656.29-Pos, 656.57-Pos Xie, Y., 479-Pos Xie, Y., 634-Pos Xie, Y., 2169-Plat Xie, Z., 308-Pos Xin, H., 1941-Pos Xin, H., 1943-Pos Xin, H., 2620-Pos Xing, J., 780-Plat Xing, J., 2496-Pos Xiong, W, 2693-Pos Xiong, W, 2699-Pos Xiong, Y., 2048-Pos Xu, C., 1013-Pos Xu, C.Z., 756-Plat Xu, H., 2830-Pos Xu,J., 153-Pos Xu, J., 341-Pos Xu,J., 1743-Pos Xu, L., 520-Pos Xu, L., 817-Pos Xu, M., 1548-Pos Xu, Q., 665-Plat Xu, Q., 1227-Pos

2607, 2608, 2609, 2610, 2611, 2612, 2613, 2614, 2615, 2616, 2617,2618,2619
9C - 1238, 1239, 1240, 1241, 1242, 1243, 1244
9D - 1245, 1246, 1247, 1248, 1249, 1250, 1251, 1252, 1253, 1254,1255,1256
9E- 1505, 1506, 1507, 1508, 1509, 1510, 1514, 1515, 1516, 1517, 1518, 1519, 1520, 1524, 1525, 1526, 1527, 1528, 1529, 1530, 1534, 1535, 1536, 1537, 1538, 1539, 1540, 1544

9F - 656.58, 1280, 1281

1511, 1521, 1531, 1541,

1512, 1522, 1532, 1542,

1513, 1523, 1533, 1543,
9G - 655 9H - 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655.07, 91 - 655.01, 655.02, 655.03, 655.04, 655.05, 655.06, 655.08, 655.09, 655.1, 655.11, 655.12, 655.13, 655.14, 655.15, 655.16, 655.17, 655.18, 655.19, 655.2, 655.21, 655.22, 655.23, 655.24, 655.25, 655.26, 655.27, 655.28, 655.29, 655.3, 655.31, 655.32, 655.33, 655.34, 655.35, 655.36, 655.37, 655.38, 655.39, 655.4, 655.41, 655.42, 655.43, 655.44, 655.45, 655.46, 655.47, 655.48, 655.49, 655.50, 655.51, 655.52, 655.53 9J - 1566, 1567, 1568, 1569, 1570, 1571, 1572, 1573, 1574, 1575,1576,1577,1578,1579,1580

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Bioenergetics Biophysical Theory/Modeling * Cell Biophysics * Channels, Receptors and Transporters * Electrophysiology * Membranes * Musde & Contractility * Nucleic Acids * Photobiophysics Proteins Spectroscopy Supramolecular Assemblies
BJ Online is a benefit ofmembership to the Biop/ysica Societyt Members may puehaste nominalfee. The Society also offers a number ofother benefit to members, inehldinr

Letters to the Editor

Table Concentrations ofpotassium in serum and plasma compared with that ofplasma from whole blood collected into Concord Pulsator syringes

Subject

original and review papers with selected illustrations, accompanied by introductory or summarising editorial comments.

Serum (mmol/l)

3-8 4-3 4-5 4-2 4-2

Plasma (mmol/l)

3-6 4-1 4-2 3-9 4-4
Dangerous hyperkalaemia after analysis of
arterial blood sample Despite the known hazards of measuring electrolytes in heparinised arterial blood samples taken for blood gas analysis,'3 we report a case of a patient whose arterial sample was used for measurement of electrolytes which led to incorrect and potentially dangerous treatment being given. We also report the use of the same commercially available arterial blood sampling syringe on the measurement of electrolytes in samples taken from five healthy subjects. An arterial blood sample was collected in the coronary care unit from a 74 year old man with acute left ventricular failure using a Concord Pulsator syringe (Concord Laboratories Ltd, Folkestone, Kent). Blood gas and electrolyte analyses requested on this sample gave the following results: pH 7-06, Pmm Hg, pCOmm Hg, base excess - 12, potassium concentration mmol/l. The patient was given 60 mmol of potassium over two hours by infusion in 500 ml of 5% dextrose. Eight hours later a further blood sample was collected. Analysis of this sample (SMAC 1, Technicon, Tarrytown, New York) gave a serum potassium concentration of mmol/l which was confinned by a repeat sample. There had been no deterioration in the patient's renal function since admission. Subsequently, venous blood samples were also collected from five healthy subjects using Concord Pulsator syringes. One syringe had been correctly primed by expulsion of heparin diluent. The heparin diluent was not expelled from the other. Venous blood was also collected into a plain glass tube and into a lithium heparin tube. Potassium concentrations were measured in the serum or plasma by indirect ion selective electrode (SMAC 1, Technicon, Tarrytown, New York). The table shows the results. Compared with serum, there was an apparent fall in potassium concentration of up to mmol/l when the syringe had been correctly primed in all samples but one. When the heparin diluent had not been expelled a fall in potassium concentration of
In the main it is an interesting exercise. The editors from the University of North Carolina at Chapel Hill give a fair commen3-4 2-9 tary, although I do not understand how the 3-8 3-2 concept that liposarcomas of the breast are 4-0 3-4 3-7 3-7 examples of metaplastic breast carcinomas 4-4 3-6 arose, and the abstracts themselves are easily assimilable. My only criticism is the lack of a general review of the events in the period up to mmol/l occurred. A smaller but 1986 to mid 1987 which is the year covered similar apparent fall in potassium concentra- by this book. tion occurred on comparison with plasma JD DAVIES samples. Heparin interference in the measurement of this patient's potassium concentration was unlikely to have occurred as the heparin concentration in the incorrectly primed syringe (about 140 U/mL), would Aociation of Clinical Pathologists not have been high enough to interfere in ion selective electrode measurement.2 The potassium concentrations in the JUNIOR MEMBERSHIP samples from the healthy subjects indicated that the patient's initially low potassium concentration was due to failure to expel the Junior Membership of the Association of heparin diluent from the syringe. It is clear, Clinical Pathologists is available to however, that even when the syringe was trainees in all branches of pathology for used correctly a considerable fall in up to six years or until they attain measured potassium concentration could consultant status. The annual subscripstill occur. tion is 18, which may be claimed against WD NEITHERCUT tax. JM ORRELL All Junior Members receive monthly Department ofPathological Biochemistry, copies of the Journal of Clinical PathWestern Infirmary, ology. Other benefits include membership Glasgow GIl 6NT. of the Junior Members' Group and a References regular junior members' newsletter, the ACP Newsletter, and all the documents 1 Shek CC, Swaminathan R. Errors due to regularly sent to full members of the heparin in the estimation of plasma sodium Association. These include the twice and potassium concentrations. Intensive yearly summary of pathology courses Care Med 1985;11:309-11. 2 Mann SW, Green A. Interference from heparin included in the ACP Postgraduate in commercial heparinised tubes in the Education Programme. measurement of plasma sodium by ion selecFor Junior Membership apply to: Dr JD tive electrode: a note of caution. Ann Clin Davies, Education Secretary, University Biochem 1986;23:355-6. Department of Pathology, Bristol Royal 3 Widdowson G. Determination of pH, pCO2, P02, sodium and potassium in the same Infirmary, Bristol BS2 8HW. whole blood sample. Ann Clin Biochem 1987; 24:641.

TZIPORI S,

WREGHITT' TG, 660*, 1135 (L)

WRIGHT DH,

UCHINO H, 763 UNADKAT P, 806 UNDERWOOD JCE, 444,1273 UPSON R, 80 URQUHART GED, 597 (L)

WRIGHT ED, 809 (L)

YAMABE H, 320
YAMAGUCHI K, 57* YANAGIHARA E, 705 (L) YOSHIDA Y, 763*
YOUNG BD, 1125 YUEN WC, 553* YULE A, 806 ZALOUDIK J, 1078'
UTTLEY D, 38 VAIRA D, 355* (L), VAN GALEN EM, 21

812* (L)

STILWELL JA, 235* (L) STOBBERINGH E, 85 STODDART RW, 1017 STOKER M, 113 (L) STOKES H, 260 STOLK K, 644
VAN HASSELT CA, 1332 VAN HERWEGHEM J-L, 158 VAN SPREEUWEL JP, 85, 1313 VAN VOOREN Jp, 62 VARIEND s, 598 (L), 742* VERGANI D, 143, 1114 VERSLUIS DJ, 49* VILLANI L, 178 VON WULFFEN H, 653*
ZANEN HC, 826 ZANEN-LIM OG, 826* ZAROD AP, 280*, 284 ZAuL D, 722 ZERBINI M, 722 ZUK JA, 410*

ZUK Ri, 1277*

ZWEYGBERG WIRGART B, 1005

Volume 41: Subject index

Abbreviations:-"(L)" refers to letters to the Editor
ABO(H) antigenicity in transitional cell carcinoma of bladder, correlation with urine cytology, 538 Abortion, detection of Chlamydia trachomatis in women seeking, comparison between cell culture and serology for, 89 Achromobacter xylosoxidans, nosocomial outbreak associated with diagnostic contrast solution (L), 920 Acid phosphatase activity, mononuclear cells positive for, in bone marrow in renal osteodystrophy, nature of, 277 Adenocarcinoma, colonic and ovarian, differential diagnosis between, statistical evaluation of antigen profiles in, 644 -, colorectal, degree of differentiation, multivariate analysis of influence on survival, 532 , -, survival in, influence of mucinous components, multivariate analysis, 1068 -in situ and epithelial membrane and 3-fucosyl-N-acetyllactosamine antigen expression in cervix uteri, 547 , tumour marker immunoreactivity in, 1064 AIDS, microsporidiosis in British patient with (L), 477 Mycobacterium avium-intracellulare isolation from blood in, comparison of methods, 687 , virus, See HIV AIDS-related complex in haemophilia, high dose immunoglobulin treatment for (L), 111 Alanine transferase assays, microtitre plate method for, 918 Albumin and retinol-binding protein, urinary excretion during normal pregnancy, 1089 Alcohol abuse, chronic, parotid and submandibular glands in, histological analysis at necropsy, 837 Alkaline phosphatase activity, leucocytic, as marker of evolution in leprosy (L), 920 histochemistry, specific diagnostic features of microvillus inclusion disease shown by, mW - isoenzyme activity in serum, determination of, quantitative method for, with estimation of intestinal component, 202 Aluminium demonstration in iliac bone, aluminon and solochrome azurine staining correlated with flameless atomic absorption spectrophotometry for, 1171 Alzheimer's disease, unchanged plasma fibronectin concentrations in (L), 476 Amiloride prevention of amphotericin B related hypokalaemia in neutropenic patients, 494 Amoebiasis and giardiasis, acute, faecal preservation and staining methods in diagnosis of, evaluation of, 694 Amphotericin B, hypokalaemia related to, prevention by amiloride in neutropenic patients, 494 Ampicidlin, Haemophilus influenzae resistant to, detection in UK laboratories, 716 Amyloid A protein and apolipoproteins A-I and B associated with high density lipoprotein during course of acute myocardial infarction, 1263 Amyloidosis, osteoarticular, associated with haemodialysis, immunoultrastructural study of case of, 158 Anabolic steroids, hepatic tumours in athlete induced by, 441 Anaemia, aplastic, severe, after parvovirus infection in absence of underlying haemolytic anaemia (L), 1242 Anaemias, myelodysplastic, refractory, with hypocellular bone marrow, 763 Anaerobes, isolation of, increase at low cost (L), 595 Angiofollicular lymph node hyperplasia (L), 353 Anorexia nervosa, vitamin D deficiency and low osteocalcin concentrations in, 195

Antibiotics, enterobacteriaceae isolates from urine resistant to, biochemical identification of, combination of six conventional tests for, 1010 Antibodies, anti-glomerular basement membrane, circulating, routine and rapid ELISAs for, 163 , anti-streptolysin 0, determination of, comparison of latex and haemolysin tests for, 1330 , IgA, to Pseudomonas aeruginosa in sera in cystic fibrosis, ELISA to detect, 1130 monoclonal. See Monoclonal antibodies red cell, screening for, polybrene technique using microplates for, 556 -to dsDNA, detection of, evaluation of five commercial kits for, 1223 to HTLV-I in systemic lupus erythematosus, detection of, comparison of immunoperoxidase staining with indirect immunofluorescence, ELISA, and Western blotting assays for, 57 Antibody, HIV, in laboratory reagents (L), 813 , IgG, to Toxoplasma gondii, ELISA compared with dye test for detection of, 802 monoclonal. See Monoclonal antibody , rabbit, polyclonal, specificity problem of (L), 705 to cytomegalovirus in donor plasma, ELISA for, 1233 to HTLVI, comparison of assays, 700 Antiepileptic drugs, zinc concentrations in leucocytes of patients receiving, 553 Antigen, carcinoembryonic, CA125, CA19-9 and h-i n milk fat globule membrane antigen, expression in ovarian tut ours, 260 D5, in normal breast, benign breast disease, at, I mammary carcinoma in situ, immunohistochemical study of, 448 , LI, myelomonocytic, formalin resistant, detection of, and Mac 387 antibody, 963 , neuroendocrine differentiation, monoclonal antibody against, detection of small cell lung cancer metastases in bone marrow aspirates using, 273 profiles, statistical evaluation of, in differential diagnosis between colonic and ovarian adenocarcinomas, 644 , T3 (CD3), antisera against epitopes resistant to denaturation on, detection of reactive and neoplastic T cells in paraffin embedded biopsy specimens by, 121 , tissue type plasminogen activator, and activity in sickle cell disease, 490 Antigens, bacterial, in blood cultures and cerebrospinal fluid, rapid identification of, 691 , epithelial membrane and 3-fucosyl-N-acetyllactosamine, expression in cervix uteri, and adenocarcinoma in situ, 547 HLA-DR. See HLA-DR antigens liver membrane, identification in tissue sections, sensitive avidin biotin based technique for, 1217 MHC class II. See MHC class II antigens tumour associated, in diagnosis of serous effusions, 633 Anti-glomerular basement membrane antibodies, circulating, routine and rapid ELISAs for, 163 Anti-inflammatory drugs, non-steroidal, "diaphragm disease" of small intestine induced by, pathology of, 516 Antimicrobial susceptibility testing of Neisseria gonorrhoeae, trial for UK national external quality assessment scheme for microbiology,

Colonic and ovarian adenocarcinomas, differential diagnosis between, statistical evaluation of antigen profiles in, 644 Colorectal adenocarcinoma, degree of differentiation, multivariate analysis of influence on survival, 532 -, survival in, influence of mucinous components, multivariate analysis, 1068 cancer, new prognostic classification and reporting (L), 358 Complement components. See C3; C4; C4d pathway, classical, activation of, assessment by laser nephelometric technique measuring C4d, 143 Computers. See Microcomputers Connective tissue cells containing factor XIII subunit a, characterisation of, 49 Contamination, environmental, in clinical laboratory due to "Centrifichem" centrifugal analyser using double fluorescent/bacterial tracer, 1229 Contrast solution, diagnostic, nosocomial outbreak ofAchromobacter xylosoxidans associated with (L), 920 Corrections, 708, 924 Coulter Plus IV leucocyte volume analysis instrument, sensitivity in identification of blast cells in peripheral blood (L), 1136 C-reactive protein in serum in infective endocarditis, 44 Creatinine determination in plasma, enzymatic method for, evaluation of, 576 Crohn's disease and ulcerative proctocolitis, polymorphonuclear leucocytes in, association between enhanced adherence to nylon fibre and disease variables, 241 , mild, reduced selenium concentrations in, 198 Cryoprecipitate, freeze dried, Factor VIII:C recovery from, influence of heat treatment, 945 Cryptosporidium oocysts, shedding of, in immunocompetent patients,
1104 Cyclosporin, histological lesions associated with, incidence and reversibility in one year old kidney transplants, 498 Cystic fibrosis, antipseudomonal IgA antibodies in sera in, ELISA to detect, 1130 -1, neonatal screening in Wales and West Midlands for, evaluation of immunoreactive trypsin test in, 726 ovarian tumours, sarcoma-like nodules in (L), 1028 Cysts, odontogenic, fluid from, lactoferrin in aspirates of, 1117 Cytokeratin proteins in squamous and transitional cell lesions of urinary tract, immunohistochemistry of, 1288 Cytology, cervical, quality assessment in, pilot study, 215 Cytomegalovirus and host balance, impairment in elderly, 722 antibody in donor plasma, ELISA for, 1233 detection by in situ hybridisation and immunohistochemistry using monoclonal antibody CCH2, comparison of, 1005 infection, primary, after bone marrow transplantation, prevention with seronegative blood products, 948 infections in heart and heart-lung transplant recipients, 660 Cytopenias, immune-mediated, intravenous Ig in, mechanism of action (Review article), 1249
D5 antigen in normal breast, benign breast disease, and mammary carcinoma in situ, immunohistochemical study of, 448 De-Nol. See Bismuth subcitrate, colloidal Des-gamma-carboxyprothrombin for diagnosis of hepatoblastoma

Desk top analysers, use for "near patient" testing, comparison of hospital staff performance in, 223 Diabetes, insulin dependent, low serum C4 haemolytic function in, 1114 type II, calcium and magnesium homeostasis in, effect of improved glycaemic control, 933 with advanced nephropathy, serum laminin PI concentrations in patients with, 929 Diagnostic equipment, extralaboratory, and involvement of laboratories with, 925 "Diaphragm disease" of small intestine induced by non-steroidal antiinflammatory drugs, pathology of, 516

(L), 356

Subject index Digestive. See Gastrointestinal Dipstick screening for bacteriuria (L), 112, 113 Diverticular disease of right colon (L), 1025 Diverticulitis, bronchial, complicating bronchial asthma, 2 cases of (L), 921 DNA amplification in HIV detection (L), 1242 -content and cell cycle distribution of erythroid and myeloid cells from bone marrow, assessment of, 1120 and MHC class II antigen expression in malignant melanoma, relation to clinical course, 1078 -, double stranded, antibodies to, detection of, evaluation of 5 commercial kits for, 1223 extraction from small sections of frozen tissue with simultaneous histological examination, 1125 flow cytometry of urinary bladder carcinoma, prognostic importance of, retrospective study, 21 human and viral, detection of, hybridisation in situ in formalin fixed, paraffin wax embedded liver specimens using biotinylated probes for, 793 hybridisation in situ, parvovirus detected by, as cause of hydrops fetalis, 381 -of cervical scrapes, comparison with cytological findings in Papanicolaou smears, 296 image cytometry in myelodysplasia, diagnostic and prognostic value, 604 of human papilloma virus type 6, laryngeal papilloma associated with, malignant progression of, 280 sequences of human papillomavirus, detection by in situ DNADNA hybridisation in cervical intraepithelial neoplasia and invasive carcinoma, 289 stemline and prognosis in pure and mixed mucinous breast carcinomas, 300 Down's syndrome and megakaryoblastic leukaemia, dicentric chromosome in bone marrow of child with, 378 Duodenal ulcer, Campylobacter colonisation, and changes in gastric mucosa (L), 1027 Dye test for detection of antitoxoplasma IgG, comparison with ELISA, 802 Dysfibrinogenaemia, acquired, masquerading as disseminated intravascular coagulation in acute pancreatitis, case of, 615 Dyspepsia, non-ulcer, Campylobacter associated gastritis in, 85 Dysplasia of bladder, primary, clinicopathological importance of, 1277
, staphylococcal, new type of, case of (L), 809 Endoscopic biopsy, use for diagnosis ofcongestive gastropathy, 1187 Enterobacteriaceae isolates from urine resistant to antibiotics, biochemical identification of, combination of 6 conventional tests for, 1010 Enteropathy, gluten sensitive, adult, slow and incomplete histological and functional recovery in, 886 Enteroviruses, non-polio, and motor neurone disease (L), 596 Enzymatic method for determination of creatinine in plasma, evaluation of, 576 Enzyme immunoassay (Chlamydiazyme) for detection of Chlamydia trachomatis in genital tract specimens, evaluation of (L), 113, 114 limitations in general hospital laboratories (L), 357 microplate reader, immunoturbidimetric assay for rheumatoid factor using, 228 linked immunosorbent assay(s). See ELISA(s) Epithelial membrane and 3-fucosyl-N-acetyllactosamine antigen expression in cervix uteri, and adenocarcinoma in situ, 547 Erythrocyte. See Red cell Erythroid and myeloid cells from bone marrow, DNA content and cell cycle distribution of, assessment of, 1120 Erythropoiesis and zinc supplementation in elderly (L), 706 Escherichia coli 0157 producing verotoxin, detection in clinical microbiology laboratory, and haemorrhagic colitis, 80; (L), 923 0157:H7 cerebral infection in humans and gnotobiotic piglets, 1099 Ewing's sarcoma of childhood, nucleolar organiser regions in, prognostic importance of (L), 232 Factor VII, human, monoclonal antibodies to, in one step immunoradiometric assay for VII:Ag, 337 -, , , in production of immunodepleted plasma for VII:C assays, 342 VIII deficient plasma, artificial, preparation with monoclonal antibodies and use in one stage coagulation assays, 562 VIII:C recovery from freeze dried cryoprecipitate, influence of heat treatment, 945 XIII subunit a, connective tissue cells containing, characterisation of, 49 Factors VII and X with triglyceride rich lipoproteins, association between, 940 Faecal preservation and staining methods in diagnosis of acute amoebiasis and giardiasis, evaluation of, 694 specimens, Campylobacterjejuni isolation from, improved blood free selective medium for (L), 704 , detection of Clostridium perfringens enterotoxin in, evaluation of ELISA, RPLA, and Vero cell assays for, 458 Faecaliths, parasites in (L), 232 Faeces, Campylobacter jejuni isolation from, enrichment culture necessary for? (L), 1135 Fat, brown, and sudden infant death (L), 597, 598 Ferritin in cerebrospinal fluid for differentiation between central nervous system haemorrhage and traumatic spinal puncture (L),

HMFG membrane antigen, CEA, CA125 and CA 19-9, expression in ovarian tumours, 260 HMFG1 expression in breast carcinoma, quantitation and association with survival, 875 HMFG-2 as prognostic indicator in superficial bladder cancer, 1191 Hodgkin's disease, flow cytometric study, 365 Hospital staff performance in use of desk top analysers for "near patient" testing, comparison of, 223 workers, medical and paramedical, in general hospital in Zimbabwe, risk of hepatitis B infection among, 334 HTLV-I antibodies in systemic lupus erythematosus, detection of,
Haemangioblastoma, cerebellar, primary, differential diagnosis from metastatic renal carcinoma, immunohistochemical methods in, 861 Haematological abnormalities in HIV disease (Review article), 711 Haematology, use of dark ground microscopy in, 786 Haemodialysis, osteoarticular amyloidosis associated with, immunoultrastructural study of case of, 158 Haemodilution, correction of plasma protein concentrations for (L),
Haemoglobin electrophoretic pattern, normal, in patient with sickle cell disease and end stage renal failure (L), 355 , glycated, and glycated protein (fructosamine) and glucose concentrations in necropsy blood samples, 415 Haemolysin and latex tests for determination of anti-streptolysin 0 antibodies, comparison of, 1330 Haemolytic function of C4 in serum, low, in insulin dependent
comparison of immunoperoxidase staining with indirect immunofluorescence, ELISA, and Western blotting assays for, 57 , antibody to, comparison of assays, 700 Human immunodeficiency virus. See HIV milk fat globule. See HMFG T cell leukaemia virus I. See HTLV-I Hybridisation in situ in formalin fixed, paraffin wax embedded liver specimens using biotinylated probes for detection of human and viral DNA, 793 non-isotopic, in detection of nucleic acid in cervix, updated protocol for, 897 Hydrogen breath test, interpretation of, effect of oral microflora (L),
1244 Hydrops fetalis, parvovirus detected by in situ DNA hybridisation as cause of, 381 5-Hydroxytryptamine, immunological detection by monoclonal antibodies as aid to diagnosis of carcinoid tumours, comparison with standard silver stains, 265 Hyperkalaemia, dangerous, after analysis of arterial blood sample (L), 1339 Hypertension, systemic, immune complexes in choroid plexus in (L), 1245 Hypocellular bone marrow in refractory myelodysplastic anaemias, 763 Hypokalaemia related to amphotericin B, prevention by amiloride in neutropenic patients, 494 Iliac bone, aluminium demonstration in, aluminon and solochrome azurine staining correlated with flameless atomic absorption spectrophotometry for, 1171 Image analysis in quantitation of intestinal metaplasia of stomach, 799 Immune complexes in choroid plexus in systemic hypertension (L), 1245 Immune-mediated cytopenias, intravenous Ig in, mechanism of action (Review article), 1249 Immunoassays, duplication of, still necessary?, 989 Immunoblastic lymphoma, positive, and terminal deoxynucleotidyl transferase (L), 354 Immunoblot analysis of immune response to Campylobacter pylori and clinical associations, 653 fingerprinting of coagulase negative staphylococci, 103 Immunochemistry and electron microscopy of head and neck rhabdomyoma, 1058 Immunocytochemical and ultrastructural study of case of invasive cribriform breast carcinoma, 17 detection of tumours of neuroectodermal origin, 586; Erratum, 924 study of cellular immune response in meningiomas, 314 Immunocytochemistry, role in diagnostic pathology, information from necrotic tissue (L), 478 Immunoelectrophoresis, two dimensional, in study of von Willebrand factor, comparison with multimer analysis, 346 Immunoglobulin A antibodies to Pseudomonas aeruginosa in sera in cystic fibrosis, ELISA to detect, 1130 D myeloma presenting as diffuse osteosclerosis, case of, 486 G antibody to Toxoplasma gondii, ELISA compared with dye test for detection of, 802 serological response to Branhamella catarrhalis in acute bronchopulmonary infections, 329 gene rearrangement and antigenic profile confirming B cell origin of primary cerebral lymphoma, 128 , intravenous, in immune-mediated cytopenias, mechanism of action (Review article), 1249 treatment, high dose, for AIDS-related complex in haemophilia (L), 111 Immunohistochemical methods in differential diagnosis between primary cerebellar haemangioblastoma and metastatic renal carcinoma, 861

staining panel for characterisation of ovarian neoplasms, 12 study of D5 antigen in normal breast, benign breast disease, and mammary carcinoma in situ, 448 of 22 cases of thymoma, 1297 Immunohistochemistry, diagnostic, tumour multiblock as aid in (L), 1337 of cytokeratin proteins in squamous and transitional cell lesions of urinary tract, 1288 Immunohistological demonstration of progesterone receptor in breast carcinomas, correlation with radioligand binding assays and oestrogen receptor immunohistology, 444 determination of proliferative activity in seminomas, 304 Immunological abnormalities in myelodysplastic syndromes (L), 922 Immunology, cervical, local, choice of control patients in studies of (L), 1138 Immunoradiometric assay, one step, for factor VII antigen with monoclonal antibodies to human factor VII, 337 Immunoreactive trypsin test for cystic fibrosis, evaluation in neonatal screening in Wales and West Midlands, 726 Immunoreactivity with tumour markers in adenocarcinoma, 1064 Immunoturbidimetric assay for rheumatoid factor using enzyme immunoassay microplate reader, 228 assays for serum apolipoproteins Al and B using Cobas Bio centrifugal analyser, 471 Immuno-ultrastructural study of case of osteoarticular amyloidosis associated with haemodialysis, 158 of circulating lymphocytes in 50 cases of HIV infection, 62 Infant death syndrome, sudden, and brown fat (L), 597 , , bacterial isolates from cases of, lethal challenge of gnotobiotic weanling rats with (L), 707 Inflammation, acute, simultaneous, in entopic and ectopic pancreas, case of, 430 Inflammatory change in rectal biopsy specimens and colitis, statistical approach, 72 response and changes in acute phase proteins, selection of laboratory tests for monitoring, ICSH guidelines, 1213 Insulin, diabetes dependent on, low serum C4 haemolytic function in, 1114 Intestinal component of serum alkaline phosphatase isoenzyme activity, estimation of, 202 markers, expression in gastric carcinoma, light and electron microscopic study using BD-5, 178 metaplasia of stomach, quantitation using image analysis, 799 Intestine, small, "diaphragm disease" of, induced by non-steroidal anti-inflammatory drugs, pathology of, 516 , , volvulus of, fetal (L), 594 Intracranial pressure, high, fatal non-missile head injury without, brain damage in, 34 Intralipid and lipaemic serum interference (L), 1026 Intraplacental choriocarcinoma, 2 cases of, 1085 Intravascular coagulation, disseminated, in acute pancreatitis, acquired dysfibrinogenaemia masquerading as, case of, 615 , necropsy evidence of, and coagulation variables before death in patients in intensive care units, 138 Intrinsic factor and binding antibody to IF, rapid protein A assay for,

1026 Lipopolysaccharide profiles of serologically identical Legionella pneumophila serogroup 6 strains, differences in, 826 Lipoprotein, high density, amyloid A protein and apolipoproteins A-I and B associated with, during course of acute myocardial infarction, 1263 Lipoproteins, triglyceride rich, factors VII and X with, association
Major histocompatibility complex. See MHC Malthus microbiological growth analyser in automated screening of blood cultures, 671 Marrow. See Bone marrow Maternal deaths, confidential enquiry into, role and importance for pathologists, 820 Megakaryoblastic leukaemia and Down's syndrome, dicentric chromosome in bone marrow of child with, 378 Melanoma, malignant, DNA content and MHC class II antigen expression in, relation to clinical course, 1078 , , primary, of gall bladder, case of, 1073 Meningiomas, cellular immune response in, immunocytochemical study of, 314 Methyl group of hepatic 5-methyltetrahydofolate, in vivo oxidation of,
Mucin glycoproteins, neutral and acidic sugars in, assessment of relative quantities, and cyclical changes in cervical mucins, 1021 Mucinous breast carcinomas, pure and mixed, DNA stemline and prognosis, 300 components, influence on survival in colorectal adenocarcinomas, multivariate analysis, 1068 -thyroid carcinomas (L), 234, 235 Mucosa, gastric, changes in, Campylobacter colonisation, and duodenal ulceration (L), 1027 Mucus penetration by Campylobacter pylori, improved visualisation using Brown-Hopps stain (L), 232 Multimer analysis in study of von Willebrand factor, comparison with two dimensional immunoelectrophoresis, 346 Muscle disorders with inflammatory infiltrate, skeletal muscle expression of HLA-DR antigens in, 410 Muscularis mucosae, degeneration of, familial autonomic visceral myopathy with, 424 Mycobacteriun avium-intracellulare isolation from blood in AIDS, comparison of methods, 687 Myelodysplasia, DNA image cytometry in, diagnostic and prognostic value, 604 Myelodysplastic anaemias, refractory, with hypocellular bone marrow, 763 and proliferative syndrome, red cell hypoplasia, thrombocytosis, and leucocytosis with morphology suggesting, 1168 syndromes, immunological abnormalities in (L), 922 Myeloid and erythroid cells from bone marrow, DNA content and cell cycle distribution of, assessment of, 1120 leukaemia, cells in, monoclonal antibody (NP57) against human neutrophil elastase in, 853 , chronic, bone marrow sea-blue histiocytes and Gaucher cells in, 960 Myeloma, IgD, presenting as diffuse osteosclerosis, case of, 486 Myeloproliferative disease in children, demographic study, 883 Myocardial infarction, acute, amyloid A protein and apolipoproteins A-I and B associated with high density lipoprotein during course of, 1263 Myocarditis, giant cell, associated with lymphoma (L), 598 Myoepithelial sialadenitis and chronic sialadenitis (sialolithiasis), clinicopathological study, 403 Myopathy, distal, recessive, autosomal, 5 cases of, 188 -, visceral, autonomic, familial, with degeneration of muscularis mucosae, 424

MHC class II antigens, expression and DNA content in malignant melanoma, relation to clinical course, 1078 by Langerhans' cells in cervical intraepithelial neoplasia, 253 Microbact-24E bacterial identification system, evaluation of, 910 Microcomputer data base ("TOXBASE") for post mortem toxicology, 1240 software to facilitate costing in pathology laboratories (L), 235 system using IBM PC and dBaseII for histopathology, 220 Microcomputers, statistics on, non-algebraic guide to use: 1, Data handling and preliminary analysis, 1033; 2, Confidence intervals and significance tests, 1148; 3, Analysis of variance and distribution-free methods, 1256 Microflora. See Bacterial flora a,-Microglobulin. See Protein HC f2-Microglobulin expression in liver after liver transplantation, 1049 Microscopy, dark ground, use in haematology, 786 Microsporidiosis in British patient with AIDS (L), 477 Microtitre plate method for alanine transferase assays, 918 Microvillus inclusion disease, specific diagnostic features shown by alkaline phosphatase histochemistry, 880 Microwave irradiation of human brain tissue for production of microscopic slides within one day, 590 Milk fat globule, human. See HMFG Monoclonal antibodies, immunological detection of 5-hydroxytryptamine by, as aid to diagnosis of carcinoid tumours, comparison with standard silver stains, 265 , Listeria monocytogenes shown in necropsy tissue samples by, 983 , preparation of artificial factor VIII deficient plasma with, and use in one stage coagulation assays, 562 -tto human factor VII in one step immunoradiometric assay for VII:Ag, 337 in production of immunodepleted plasma for VII:C assays, 342 See also BD-5; EBM/1 1; HMFG; Mac, 387 antibody against neuroendocrine differentiation antigen, detection of small cell lung cancer metastases in bone marrow aspirates using, 273 in rapid diagnosis of whooping cough, 573 (BFI) in definitive identification of human T cells in formalin fixed paraffin wax embedded tissue, 900 (CCH2), detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using, comparison of, 1005 (NP57) against human neutrophil elastase in normal and leukaemic myeloid cells, 853 (YI/82A) with specificity towards peripheral blood monocytes and tissue macrophages, 753 Monocytes of peripheral blood and tissue macrophages, monoclonal antibody (Yl/82A) with specificity towards, 753 Mononuclear cells positive for acid phosphatase activity in bone marrow in renal oesteodystrophy, nature of, 277 Morphometry in cytological preparations for diagnosis of follicular non-Hodgkin's lymphomas, 370 Motor neurone disease and non-polio enteroviruses (L), 596

Nasopharyngeal tuberculosis, diagnosis by detection of tuberculostearic acid in formalin fixed, paraffin wax embedded tissue biopsy specimens, 1332 Neck and head rhabdomyoma, immunochemistry and electron microscopy of, 1058 Neisseriagonorrhoeaeantimicrobial susceptibility testing, trial for UK national external quality assessment scheme for microbiology, 97 , susceptibility testing to penicillin and spectinomycin in diagnostic laboratory, 978 Neonatal intensive care unit, comparison of Bactec and Signal blood culture systems in (L), 704 screening for cystic fibrosis in Wales and West Midlands, evaluation of immunoreactive trypsin test in, 726 Neoplasia, intraepithelial, cervical, and invasive cervical carcinoma, human papillomavirus DNA sequences in, detection by in situ DNA-DNA hybridisation, 289 ,91 -, -9expression of MHC class II antigens by Langerhans' cells in, 253 Neoplasms, intraepithelial,, cervical, nucleolar organising regions in, 1200 , ovarian, characterisation of, immunohistochemical staining panel for, 12 , thyroid, argyrophilic nucleolar organising region-associated protein enumeration in assessment of, limited value of (L), 1136 Nephropathy, advanced, diabetes with, serum laminin P1 concentrations in patients with, 929
Nervous system, central, haemorrhage of, and traumatic spinal puncture, differentiation between, cerebrospinal fluid ferritin for (L), 809 Neuroblastoma, differentiation of, degree of, and mean numbers of nucleolar organiser regions, comprative study of, 527 Neuroectodermal origin, tumours of, immunocytochemical detection of, 586; Erratum, 924 Neuroendocrine differentiation antigen, monoclonal antibody against, detection of small cell lung cancer metastases in bone marrow aspirates using, 273 Neutropenia, amphotericin B related hypokalaemia in patients with, prevention by amiloride, 494 preceding acute lymphoblastic leukaemia, case of (L), 703 Neutrophil chemotaxis and leukotriene B4 synthesis in chronic granulocytic leukaemia, 1163 -elastase associated with elastic tissue in emphysema?, 435; (L), 1337, 1338 , human, in normal and leukaemic myeloid cells, monoclonal antibody (NP57) against, 853 Niemann-Pick disease, late, with neurovisceral storage, classification problem in case of, 619 Nitroimidazole resistance in Campylobacter pylori, prevention by coadministration of colloidal bismuth subcitrate, clinical and in vitro studies, 207 Notices, 120, 240, 360, 480, 600, 816, 924, 1031, 1140, 1248, 1338 NSAIDs. See Anti-inflammatory drugs, non-steroidal Nucleic acid detection in cervix by non-isotopic in situ hybridisation, updated protocol for, 897 Nucleolar organiser region-associated proteins, argyrophilic, enumeration of, limited value in assessment of thyroid neoplasms (L), 1138 regions in cervical intraepithelial neoplasia, 1200 in embryonal rhabdomyosarcoma, prognostic importance of (L), 477 in Ewing's sarcoma of childhood, prognostic importance of (L), 232 in fibrous proliferations of childhood and infantile fibrosarcoma, 31 in lymphocytes in chronic lymphocytic leukaemia (L), 1336 - in normal, cirrhotic, and carcinomatous livers, 1044 mean numbers of, and degree of differentiation of neuroblastoma, comparative study of, 527

Radioimmunoassay in monitoring of treatment of human cerebral gliomas with bleomycin entrapped within liposomes, 38 Radiology, post mortem, in children with congenital heart disease, 830 Reagents, laboratory, anti-HIV positive (L), 813 Rectal and skin biopsy specimens after autologous and allogeneic bone marrow transplantation, histological features of, 148 -biopsy, smooth muscle pseudotumours as potentially confusing artefact of, 737 specimens, inflammatory change in, and colitis, statistical approach, 72
carcinoma, new prognostic classification for, feasibility of, 1273 Rectum and colon, brush cytology in ulcerative colitis as aid to cancer diagnosis, 1180 Red cell antibody screening, polybrene technique using microplates for, 556 hypoplasia, thrombocytosis, and leucocytosis with morphology suggesting myelodysplastic and proliferative syndrome, 1168 volume distribution width, increase in patients with bone marrow metastases (L), 1246 Reflectance meters, 3 types of, for self monitoring of blood glucose concentrations, laboratory assessment of, 397 Renal biopsy specimens, percutaneous, in stone formers, 971 - carcinoma, metastatic, differential diagnosis from primary cerebellar haemangioblastoma, immunohistochemical methods in, 961 failure, chronic, plasma oxalate concentration and secondary oxalosis in, 1107 , end stage, and sickle cell disease, normal haemoglobin electrophoretic pattern in patient with (L), 355 histology, postmortem, routine, cost effectiveness of (L), 233 osteodystrophy, mononuclear cells positive for acid phosphatase activity in bone marrow in, nature of, 277 transplant recipient with disseminated histoplasmosis, caecal perforation in, 992 with focal and segmental glomerulosclerosis, unusual interpodocyte cell junction in, 155 transplants, one year old, incidence and reversibility of histological lesions associated with cyclosporin in, 498 Retinol-binding protein and albumin, urinary excretion during normal pregnancy, 1089 Rhabdomyoma of head and neck, immunochemistry and electron microscopy of, 1058 Rhabdomyosarcoma, embryonal, nucleolar organiser regions in, prognostic importance of (L), 477 Rheumatoid arthritis and osteoarthritis, synovium from patients with, morphometric comparison of, 847 , synovium in, morphometric study of representative sample, 841 factor, immunoturbidimetric assay using enzyme immunoassay microplate reader for, 228 Rowmanowsky stain deposits, removal of (L), 920
Sarcoma, Ewing's, of childhood, nucleolar organiser regions in, prognostic importance of (L), 232 Sarcoma-like nodules in cystic ovarian tumours (L), 1028 Selenium concentrations, reduction in mild Crohn's disease, 198 , serum concentration associated with risk of cancer, 454 Seminomas, proliferative activity in, immunohistological determination of, 304 Septicaemia, detection of, and blood culture systems, 668 Sera, pooling for HIV testing as economical method for developing countries, 582 Serous effusions, diagnosis of, tumour associated antigens in, 633 Serum alkaline phosphatase isoenzyme activity, determination of, quantitative method for, with estimation of intestinal component, 202 -antipseudomonal IgA antibodies in cystic fibrosis, ELISA to detect, 1130 apolipoproteins Al and B, immunoturbidimetric assays using Cobas Bio centrifugal analyser for, 471 C4 haemolytic function, low, in insulin dependent diabetes, 1114 C-reactive protein in infective endocarditis, 44 laminin P1 concentrations in diabetics with advanced nephropathy, 929 selenium concentration associated with risk of cancer, 454 spreading factor. See Vitronectin Shigellaflexneri, glycocalyx in virulent and avirulent strains of (L), 810 Sialadenitis, myoepithelial and chronic sialadenitis (sialolithiasis),

clinicopathological study, 403 Sialic acid heterogeneity in ulcerative colitis, basis of, 388 Sickle cell disease and end stage renal failure, normal haemoglobin electrophoretic pattern in patient with (L), 355 -, tissue type plasminogen activator antigen and activity in, 490 Signal and Bactec blood culture systems, comparison in neonatal intensive care unit (L), 704 NR-660 blood culture systems, comparison of, 676 blood culture system in paediatrics, evaluation of, 683 Silver deposition in cervix after silver nitrate cautery, 871 stains, standard, as aid to diagnosis of carcinoid tumours, comparison with immunological detection of 5-hydroxytryptamine by monoclonal antibodies, 265 Skeletal muscle expression of HLA-DR antigens in polymyositis and other muscle disorders with inflammatory infiltrate, 410 Skin and rectal biopsy specimens after autologous and allogeneic bone marrow transplantation, histological features of, 148 Slavin, Gerard, editor, Journal of Clinical Pathology, 1978-88, 1 Small cell carcinoma of lung, detection of metastases in bone marrow aspirates using monoclonal antibody against neuroendocrine differentiation antigen, 273 Smooth muscle pseudotumours as potentially confusing artefact of rectal biopsy, 737 Solochrome azurine and aluminon staining for aluminium demonstration in iliac bone, correlation with flameless atomic absorption spectrophotometry, 1171 Spectinomycin and penicillin, susceptibility testing of Neisseria gonorrhoeae to, in diagnostic laboratory, 978 Spermatogenesis, human, shown with Arachis hypogaea or peanut lectin, 1017 Spinal puncture, traumatic, and central nervous system haemorrhage, differentiation between, cerebrospinal fluid ferritin for (L), 809 Spleen, pseudotumour of, inflammatory, case of, 734 Squamous and transitional cell lesions of urinary tract, cytokeratin proteins in, immunohistochemistry of, 1288 Staphylococcal endocarditis, new type of, case of (L), 809 Staphylococci, coagulase negative, immunoblot fingerprinting of, 103 Statistics on microcomputers, non-algebraic guide to use: 1, Data handling and preliminary analysis, 1033; 2, Confidence intervals and significance tests, 1148; 3, Analysis of variance and distribution-free methods, 1256 Steroids, anabolic, hepatic tumours in athlete induced by, 441 Stomach biopsy specimens, Campylobacter-like organisms in, retrospective study of, 1313 -, intestinal metaplasia of, quantitation using image analysis, 799 Stones. See Calculi Streptococcus pneumoniae, antimicrobial susceptibility testing of, quality assessment results, 384 from clinical isolates, penicillin sensitivity of, decrease in, incidence of, 720 Submandibular and parotid glands in chronic alcohol abuse, histological analysis at necropsy, 837 Sugars, neutral and acidic, in mucin glycoproteins, assessment of relative quantities, and cyclical changes in cervical mucins, 1021 Synovium from patients with osteoarthritis and rheumatoid arthritis, morphometric comparison of, 847 -, rheumatoid, morphometric study of representative sample, 841

Vaginal Weeksella virosa (formerly group I1f), incidence of, 211 Vasitis nodosa and associated clinical findings, 419 Vegetarian diet and cobalamin deficiency, association with tuberculosis, 759 Verotoxin, E coli 0157 producing, detection in clinical microbiology
Zinc concentrations in leucocytes of patients receiving antiepileptic drugs, 553 -supplementation and erythropoiesis in elderly (L), 706