KGO netto 5,00 1,49 5,00 1,49 5,00 1,49 1,49 1,49 1,49 1,49 1,49 2,13 2,54 2,75 3,42 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 2,12 2,12 2,12 2,12 2,12 2,12 2,12 2,12 2,12 1,63 1,59 1,46 1,58 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59 1,59
KGO brutto 6,15 1,83 6,15 1,83 6,15 1,83 1,83 1,83 1,83 1,83 1,83 2,62 3,12 3,38 4,21 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 2,61 2,61 2,61 2,61 2,61 2,61 2,61 2,61 2,1,95 1,79 1,94 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95 1,95

Strona 57 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe Kuchnie mikrofalowe MARKA LG LG LG LG MANTA MANTA MANTA MANTA MASTERCOOK MASTERCOOK MASTERCOOK MASTERCOOK MASTERCOOK MIELE MOULINEX MOULINEX MOULINEX MOULINEX MOULINEX MOULINEX MOULINEX MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT OPTIMUM OPTIMUM OPTIMUM OPTIMUM OPTIMUM OPTIMUM OPTIMUM PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC PANASONIC SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG SAMSUNG NAZWA PRODUKTU MS-2337AR MS-2337PR MS-2349HS MS-2387ARS MM414 MM415 MM460 MM461 MM-17 X MM-17GE B MM-17GE X MM-23GE B MM-23GE X M 8201-1 AFM243 Ultimys Duo Grill AFM443 Ultimys Duo Grill AFM8 AFW242 Ultimys Duo Combi MW2210 MW5310 MW7001 D70D17EP-D6 D70D17EP-D6/S D70D17P-D2 D70D17P-D2/S D80D20ESL-T5 D90D23EL-T2 WD800ESL20-SP2 WD900ESL23Q-2W MFAG 17L MFAG 20L MFAG 23 L Digital Silver MFB 17L MFE 17L MFEG 17L MFEG 23L Digital NN-CD557WEPG NN-CD757WEPG NN-CS 598 SETG NN-CS596SEPG NN-E205WBEPG NN-E235MBEPG NN-GD358WEPG NN-GD359WEPG NN-GD368MEPG NN-GD369MEPG NN-GD377SEPG NN-GD379SEPG NN-GD458WEPG NN-GD459WEPG NN-GD468MEPG NN-GD469M NN-GD556WEPG NN-GD566MEPG NN-J125MBEPG NN-J125MBETG NN-J155WBEPG NN-J155WBETG NN-K105WBEPG NN-S225MBEPG NN-S255WBEPG NN-S269MMEPG CE107BAF CE107BAF-S CE117AE-X CE1193F CE287AST GE86H GE86H-S GE89AST GE89M GE89M-B

KGO netto 0,23 0,23 0,23 0,23 0,23 0,41 0,06 0,06 0,41 0,05 0,41 0,12 0,15 0,11 0,11 0,14 0,18 0,23 0,23 0,23 0,12 0,52 0,35 0,35 0,35 0,35 0,35 0,35 0,35 0,35 0,35 0,33 0,33 0,33 0,33 0,33 0,33 0,33 0,33 0,33 0,33 0,33 0,10 0,10 0,70 0,70 0,50 1,72 1,59 2,54 2,49 2,49 2,00 0,60 0,46 0,59 0,35 0,35 0,60 0,46 0,45 0,63 0,59 0,35 0,35 0,35 0,31 0,31 0,41 0,41 0,06 0,06
KGO brutto 0,28 0,28 0,28 0,28 0,28 0,5 0,07 0,07 0,5 0,06 0,5 0,15 0,18 0,13 0,13 0,17 0,22 0,28 0,28 0,28 0,15 0,64 0,43 0,43 0,43 0,43 0,43 0,43 0,43 0,43 0,43 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,4 0,12 0,12 0,86 0,86 0,62 2,11 1,95 3,12 3,06 3,06 2,46 0,74 0,56 0,73 0,43 0,43 0,74 0,56 0,55 0,78 0,73 0,43 0,43 0,43 0,38 0,38 0,5 0,5 0,07 0,07

Strona 140 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Masaery stp Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia Maszynki do mielenia MARKA HOMEDICS HOMEDICS HOMEDICS HOMEDICS HOMEDICS LAICA LAICA MANTA MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT ROWENTA ROWENTA SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SCHOLL SEVERIN SEVERIN SEVERIN SEVERIN SEVERIN SOLAC SOLAC SOLAC SOLAC TERRAILLON TERRAILLON TERRAILLON TERRAILLON ADLER ADLER ADLER ARDO BOMANN BOSCH BRAUN BRAUN CLATRONIC CLATRONIC CLATRONIC CONCEPT DE LONGHI ELDOM ELESKO GORENJE GORENJE GORENJE HOLDEN HOLDEN HOLDEN KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD MANTA MOULINEX MOULINEX MOULINEX MOULINEX MOULINEX MOULINEX NAZWA PRODUKTU FM-100H FM-CR FM-TS9-GB FMV-300 HL-200 BF 2011 PC 1011 MM103 HX-9600 J-0112 SLT-1R SLT-335 TS 3510 Fitspa Massage TS 5510 Fitspa Reflexology DR1135E DR6624BE DR6624E DR6624GE DR6624PKE DR6653ER DR6654E DRFB7132BE DRFB7132GE DRFB7132PE DRFB7132VE DRFB7133E FM 7608 FM 7609 FM 7631 FM 7632 FM 7633 E800 E801 ME7750 ME7755 Aqua Spa 10 Aqua Spa 60 Tonic 2 Tonic 3 AD 48 B AD 48 W AD 4801 TN 21 CB 427 MFW 1501 G 3000 Power Plus G 1300 FW 2684 FW 2924 FW 3151 MM-4210 KMG 1200 M100 MM-01 MG 1000 B MG 2000 E MG 2000 TJW MM-40 MM-41 MM-42 MG 450 MG 470 MG 510 MG 700 PG 520 MM503 DKA147 Combi HV8 DKA14E DKA247 Combi HV8 DKA24E ME406185 ME41113E Charlotte HV4

KGO netto 1,33 1,17 1,17 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 2,98 0,45 0,45 0,45 0,45 1,98 2,98 2,98 2,98 1,16 1,15 1,15 1,12 1,23 1,23 1,07 1,09 1,14 1,34 1,38 1,38 1,38 1,38 1,38 1,11 1,48 1,49 1,50 0,33 0,23 0,41 0,10 0,51 0,41 0,22 0,22 0,22 0,28 0,52 0,41
KGO brutto 1,64 1,44 1,44 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 3,66 0,55 0,55 0,55 0,55 2,44 3,66 3,66 3,66 1,43 1,42 1,41 1,38 1,51 1,51 1,31 1,34 1,4 1,65 1,7 1,7 1,7 1,7 1,7 1,36 1,82 1,83 1,85 0,41 0,28 0,51 0,126 0,627 0,5 0,27 0,27 0,27 0,34 0,64 0,5

Strona 143 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne Miksery rczne MARKA BOMANN BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BRAUN BRAUN BRAUN BRAVO BRAVO CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CONCEPT CONCEPT ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELECTROLUX ELECTROLUX ELECTROLUX ELESKO ELESKO ELESKO ELTA ELTA ELTA ELTA ELTA ELTA ETA FAGOR FAGOR FAGOR FAGOR GORENJE GORENJE GORENJE HB HOLDEN HOLDEN HOLDEN HOLDEN HOLDEN HOLDEN HOLDEN HOMEFRIEND HYUNDAI HYUNDAI HYUNDAI HYUNDAI NAZWA PRODUKTU KM 1903 CB MFQ 3010 MFQ 3030 MFQ 3520 MFQ 3530 MFQ 3540 MFQ 3560 MFQ 3570 MFQ 3580 MFQ 4020 MFQ 4070 M 1000 Columbus M 1050M Columbus Multimix M 700 TSK 941 TSK 941 GST HM 2642 HM 2738 HM 2830 HM 3014 HM 302 HM 3316 HMS 2477 HMS 2739 HMS 2772 HMS 3152 HMS 3320 KM 2893 KM 3118 SR-3110 SR-3320 R100 R101 R2 R2M R3 R3M R4 R4M AHM 310 ASM 450 ASM 550 MK-01 MK-02 MK-03 HM 100/6 HM 106 HM 110/4 HM 120 HM 210 HM Fresco SP-251 SP-251 PLUS SP-350 SP-350 M 701 W ME 500 N MRP 330 EA HM 0251BS MR-40 MR-40M MR-41 MR-41M MR-42 MR-43 MR-43M HM20D11 HM628 HM638 HMB205W HMB801E

KGO netto 0,40 0,40 0,40 0,20 0,20 0,20 0,20 0,40 0,20 0,37 0,41 0,41 0,41 0,41 0,78 0,78 0,78 0,22 0,78 0,22 0,22 0,22 0,78 0,78 0,79 0,48 0,35 0,35 0,35 0,76 0,44 0,71 0,51 0,80 0,64 0,64 0,96 0,59 0,51 0,54 0,48 0,99 0,83 0,34 0,34 0,83 0,40 0,40 0,65 1,29 1,29 0,99 1,00 1,00 0,68 0,22 0,75 0,75 0,41 0,26 0,26 0,26 0,41 0,41 0,41 1,27 1,27 0,24 0,63 1,27 1,27 0,36
KGO brutto 0,49 0,49 0,49 0,24 0,24 0,24 0,24 0,49 0,24 0,45 0,5 0,5 0,5 0,5 0,96 0,96 0,96 0,27 0,96 0,27 0,27 0,27 0,96 0,96 0,97 0,59 0,43 0,43 0,43 0,94 0,54 0,87 0,63 0,98 0,79 0,79 1,18 0,72 0,63 0,67 0,59 1,22 1,02 0,42 0,42 1,02 0,49 0,49 0,8 1,59 1,59 1,22 1,23 1,23 0,84 0,27 0,92 0,92 0,5 0,32 0,32 0,32 0,51 0,51 0,51 1,56 1,56 0,3 0,77 1,56 1,56 0,44

Strona 146 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Miksery stojce Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki Mini piekarniki MARKA KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KENWOOD-AGD KRUPS MELISSA MOULINEX MOULINEX MOULINEX MPM PRODUCT MPM PRODUCT MPM PRODUCT OPTIMUM OPTIMUM PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS PHILIPS SEVERIN SEVERIN SEVERIN SEVERIN SEVERIN SIEMENS SOLAC SOLAC SOLAC SOLAC TAURUS TAURUS TIGER TIGER ZELMER ZELMER ZELMER ZELMER ZELMER ADLER ADLER ADLER ARDES ARDES ARDES ARDO ARDO ARIETE ARIETE BOMANN BOMANN NAZWA PRODUKTU BL 745 BL 760 BL 770 BL 900 BLX 50 BLX 51 BLX 54 SB 055 SB 100 SB 106 SB 245 SB 255 SB 256 SB 266 SB 277 SB 300 SB 306 SB 307 SB327 KB710D41 YD-FM-805 DAB447 Vivacio DAB547 Vivacio LM600E4E Direct Service BL-747 MBL-01M Zosia TSK-9356 RK-2010 RK-3210 HR 2000/70 HR 2020/70 HR 2061/55 HR 2074/50 HR 2084/01 HR 2084/30 HR 2084/90 HR 2094/00 HR 2161/40 HR 2170/50 HR 2800/50 HR 2860/55 SM 3713 SM 3714 SM 3715 SM 3716 SM 3717 MB 91101 B317 B714 BV5710 BV5720 Olimpia Glass Optima Legend Smoothie Maker Biay Smoothie Maker Czerwony 32Z010 Czarny 32Z010 Kremowy 32Z012 32Z013 Expressive 32Z013 Symbio AD 6001 AD 6002 AD FN 22 FN Gran Gusto Arte Bon cusine petit CB 1236 CB 1260

KGO netto 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 0,12 0,23 0,23 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,49 1,49 1,49 1,49 0,63 0,63 0,75 1,30 1,30 0,62 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,63 1,19 1,19 0,87 1,41 1,97
KGO brutto 2 0,15 0,28 0,2 1,83 1,83 1,83 1,83 0,78 0,78 0,92 1,6 1,6 0,1,46 1,46 1,07 1,74 2,42
S750T CAPTOR SJ12 WSR Jovis Wet&Dry SJ144 WSR Jowis Wet&Dry SJ6 DSW Jovis Dry SJ72 CW Jowis cyclonic SJ72 WSA Jowis Wet&Dry SJ8 DSB Jovis Dry SJ96 CO Jovis cyclonic SL 8123 Slalom SP36 DI Dry SP48 DR Dry SP48 WO Wet&Dry ST 321 EA ST 325 EC ST 345 EC Athyss SU 120 P SU 144 BR2 SU 144 CBR SU 180T HVC-601S FD-SDJ01 Marysia FD-SDJ01/R Marysia FC 6050/99 FC 6091/01 FC 6093/01 FC 6095/01 FC 6126/01 FC 6132/02 FC 6140/01 FC 6142/01 FC 6144/01 FC 6146/01 FC 6148/01 SH5051 AH 7908 AH 7909 AH 7910 AH 7912 A017E2 A017F2 A018 AE2500 AE2510 AD 70 VJ 1031 VJ 1032 VK 1011 Universis VK 1012 VK 1012 VK 3011 Carris VK 3012 Carris Plus VK 4011 Dublis Plus VK 4021 VK 4022 VK 5011 Maxis Power VK 5012 Maxis Power Plus VK 5013 Maxis Silent VK 5014 Maxis Silent Plus VK 5021 Maxis Power Cyclone VK 5022 Maxis Power Cyclone Plus VM 1021 VM 1021 VM 1021 (old) VM 1022 VM 1023 AP 51 AP Vacuum Cleaner 2799 Bagless Vacuum Cleaner BS 970 CB BS 971 CB BS 972 CB

Strona 151 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe Odkurzacze standardowe MARKA BOMANN BOMANN BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BOSCH BRAVO CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT NAZWA PRODUKTU BS 980 CB BS 985 CB BSD 2822 BSD 3030 BSG 42500 BSG 61877 BSG 62085 BSG 62282 BSG 71266 BSG 72000 BSG 72223 BSG 72230 BSG 82425 ProAnimalHair BSG 8PRO1 BSG 8PRO11 BSGL 2MOVE1 BSGL 2MOVE2 BSGL 2MOVE3 BSGL 2MOVE5 BSGL 32015 BSGL 32030 BSGL 32125 BSGL 32222 BSGL 32500 BSGL 42282 BSGL 52130 BSGL 52233 BSGL 5PRO1 BXBXBXB 4141 Gaston BS 1229 BS 1231 BS 1232 BS 1234 BS 1237 Czarny BS 1238 Czerwony BS 1238 Niebieski BS 1240 BS 1243 BS 1247 BS 1248 BS 1249 BS 1250 Granatowy BS 1250 Kremowy BS 1253 BS 1256 BS 1257 BS 1259 BS 1264 BS 1266 Czerwony BS 1267 BS 1268 BS 1272 BS 1273 Biay BS 1273 Czerwony BS 1273 Niebieski BS 1274 BS 1275 BS 900 BS 901 VP-5031 VP-5060 VP-5070 VP-5070 WH VP-9050 Comfort VP-9070 Sprinter VP-9071 VP-9080 VP-9090 VP-9141 BL

KGO netto 7,14 4,96 5,00 5,00 5,00 7,14 5,00 5,00 7,14 7,14 7,14 4,96 7,14 7,14 4,96 5,00 5,00 7,14 7,14 5,00 5,00 4,96 5,00 5,00 4,96 5,00 7,14 7,14 7,14 7,14 5,00 7,14 5,00 5,00 7,14 7,14 5,00 5,00 5,00 7,14 7,14 7,14 5,10 5,10 5,10 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27
KGO brutto 8,78 6,1 6,15 6,15 6,15 8,78 6,15 6,15 8,78 8,78 8,78 6,1 8,78 8,78 6,1 6,15 6,15 8,78 8,78 6,15 6,15 6,1 6,15 6,15 6,1 6,15 8,78 8,78 8,78 8,78 6,15 8,78 6,15 6,15 8,78 8,78 6,15 6,15 6,15 8,78 8,78 8,78 6,27 6,27 6,27 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71
EWF 12670W Time Manager EWF 127410 W Time Manager EWF 127413W Time Manager EWF 127440W Time Manager EWF 127570W Time Manager EWF 14470W Time Manager EWF 146410W Time Manager EWF 147540W Time Manager EWF 14780W Time Manager EWF 16981W Time Manager EWF 8040W Time Manager EWF 86110W Time Saver EWFM 12470W Time Manager EWFM 14480W Time Manager EWM 126410W Time Manager EWM 147410W Time Manager EWN 148640W Time Manager EWN 14991K Icon Time Manager EWN 14991W Icon Time Manager EWN 167540W Time Manager EWP 106100W EWP 106200W EWP 106300W EWP 126100W EWP 126300W EWP 86100W EWS 10070W Time Manager EWS 10170W EWS 10470S Time Manager EWS 10470W Time Manager EWS 106110W Time Saver EWS 106210W EWS 106410S Time Manager EWS 106410W Time Manager EWS 10670W Time Manager EWS 12470W Time Manager EWS 126410W Time Manager EWS 126510W Time Manager EWS 126540W Time Manager EWS 12670W Time Manager EWS 12971W Time Manager EWS 14971W Time Manager 1F-4611 1F-4613 1F-4613 X WA 40109 WA 50089 WA 50109 WA 50129 WA 60109 WA 60129 WA 60149 WA 612 SYB WA 612 SYW WA 614 SYB WA 614 SYW WA 63100 WA 63120 WA 64123 WA 64143 WA 72125 WA 72145 WA 72145 BK WA 73120 WA 73121 WA 74123 WA 74143 WA 74163 WA 75185 WS 40109 WS 40129 WS 40149

Strona 226 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu Pralki adowane od frontu MARKA GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE GORENJE HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOOVER HOTPOINT ARISTON HOTPOINT ARISTON NAZWA PRODUKTU WS 43100 WS 43121 WS 50109 WS 50129 WS 50149 WS 512 SYB WS 512 SYW WS 52105 WS 52125 WS 53100 WS 53120 WS 53121 AAA 160 AI 1040 AI 120 AIS 136 AL 1040 AL 120 AL 130 AL 80 DST 8166 P DST10146PG DYN 7124D-85S DYN 7144DP-8 DYN 8144DHC DYN 8146 P DYN 9166 PG DYN10124D DYN10124DG H 100 H 120 H 140 H 80 HN 6125 HN 6135 HNF 6127 HNF 6137 HNF 9167 HNL 6106 HNL 9126 HNS 6105 HNS 9125 HVP 16 HVP 16 ALU HVP 16-03 ALU VHD 106 VHD 146 VHD 148 VHD 166 I VHD 168 VHD 612 VHD 614 VHD 6143D VHD 616 ZI VHD 6163D VHD 814 VHD 814/3 VHD 8143ZDB VHD 9143ZD VHD 9144ZD VHD 9163ZI VHD 9164ZD VHDVHDD VHDS 610 VHDS 6103 D VHDS 612 VHDS 612 3D VHDS 612 4D VHDS 614 3ZD AQ7D 29 U (EU)/1B AQ7F 29 U (EU)

KGO netto 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 7,14 7,14 7,14 7,14 7,14 4,96 5,00 5,00 7,14 5,00 7,14 5,00 7,14 4,96 4,96 4,96 5,10 6,22 6,22 6,22 6,22 6,10 6,10 5,68 6,10 6,33 6,10
KGO brutto 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 8,78 8,78 8,78 8,78 8,78 6,1 6,15 6,15 8,78 6,15 8,78 6,15 8,78 6,1 6,1 6,1 6,27 7,65 7,65 7,65 7,65 7,5 7,5 6,99 7,5 7,79 7,5

Strona 233 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry Pralki adowane od gry MARKA BOSCH CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY CANDY ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX GORENJE GORENJE GORENJE GORENJE HOOVER HOOVER HOOVER NAZWA PRODUKTU WOT 26352 BY CT 1296 TXT CT 1496 TXT CT 1496/1 TXT CTD 1066 CTD 10662 CTD 11652 CTD 1207 CTD 1208 CTD 1266 CTD 12662 CTD 1276 CTD 12762 CTD 13652 CTD 1466 CTD 14662 CTDF 1006 CTDF 1007 CTDF 1206 CTDF 1406 CTF 1006 CTF 1206 CTG 1056 CTG 1056/1 CTG 1256 CTG 1456 CTG 1456/1 CTL 1208 CTL 1406 CTY 1046 CTY 1046/1 CTY 105 CTY 1246 CTY 1246/1 EWB 105205W Time Saver EWB 105405W Time Manager EWB 95205W Time Saver EWT 10110W EWT 10115W Time Manager EWT 10120W Time Manager EWT 10410W Time Manager EWT 10420W Time Manager EWT 105210W Time Manager EWT 105410W Time Manager EWT 105510W Time Manager EWT 10620W Time Manager EWT 10730W Time Manager EWT 13420W Time Manager EWT 135410 W EWT 135510W Time Manager EWT 13620W Time Manager EWT 136540W Time Manager EWT 136580W Time Manager EWT 136640W Time Manager EWT 13741W Time Manager EWT 13931W Time Manager EWT 9120W Time Manager EWT 9125W Time Manager EWTS 10120W Time Manager EWTS 10420W Time Manager EWTS 10620W Time Manager EWTS 13420W Time Manager EWTS 13620W Time Manager EWTS 13741W Time Manager EWTS 13931W Time Manager WT 63090 WT 63100 WT 63110 WT 63130 DYT8104D DYT8126 HNT 412/1

KGO netto 6,23 6,10 6,36 6,10 6,61 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,10 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 6,27 5,00 2,76 1,09 1,60 0,61 1,54 2,67 3,07 3,59 3,59 4,79 7,06 7,06 2,30 2,30 2,44 1,89 3,54 4,96 6,22 6,22 6,22 6,22 6,22 7,93 6,10 6,10 6,27 6,27 6,27
KGO brutto 7,66 7,5 7,82 7,5 8,13 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,5 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 7,71 6,15 3,4 1,34 1,97 0,75 1,89 3,29 3,78 4,41 4,41 5,89 8,68 8,68 2,83 2,2,32 4,36 6,1 7,65 7,65 7,65 7,65 7,65 9,76 7,5 7,5 7,71 7,71 7,71

Strona 236 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy Pralko-suszarki do zabudowy MARKA CANDY CANDY CANDY DAEWOO DAEWOO DAEWOO DAEWOO DAEWOO ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX ELECTROLUX GORENJE HOOVER HOOVER HOOVER HOTPOINT ARISTON HOTPOINT ARISTON HOTPOINT ARISTON HOTPOINT ARISTON HOTPOINT ARISTON HOTPOINT ARISTON INDESIT INDESIT INDESIT INDESIT LG LG MASTERCOOK MIELE MIELE SAMSUNG SAMSUNG SAMSUNG SIEMENS SIEMENS WHIRLPOOL WHIRLPOOL AEG-ELECTROLUX AEG-ELECTROLUX ARDO ARDO CANDY CANDY CANDY CANDY CANDY CANDY ELECTROLUX HOOVER HOOVER HOTPOINT ARISTON MIELE MIELE NAZWA PRODUKTU GOW 464 D GOW 465 D GOW 485 D DWC-LD1412S DWC-LD141RS DWC-UD1213 DWC-UD121DC DWD-E1211R EWW 12470W Time Manager EWW 126410W Time Manager EWW 14791W Time Manager EWW 148540W Time Manager EWW 168540W Time Manager WD 63110 VH W854ZD/1 VHW 964DP-37S WDYN 9646PG AQGMD 149/B (EU) AQGMD 149/B H (EU) AQM9D 49 U (EU)/B AQM9D 49 U H (EU)/B ARMXXD 109 (EU) ARMXXD 129 (EU) IWDC 7105 EU IWDE 7105 B EU WIDXL 106 WIDXL 86 F-1203CD WD-12330CDP SPFD-1064 WT 2670 WPM WT 2780 WPM WD7101CKW WD7704S8V WD8704DJF WD 12D520EU WD 14H420EU AWZ 410/D AWZ 414/D Lavamat 12843 ViT Lavamat 14710 ViT WDI 120 L WDOI 1063 S CDB 115 CDB 134-SY CDB 464-47S CDB 465 CDB 485D CIW 100 T EWX 14550W HDB 284 SY HDB 854D/1-3 CAWD 129 (EU) WT 2679i WPM WT 2789i WPM

KGO netto 0,39 0,54 0,54 0,42 0,46 0,41 0,46 0,18 0,35 0,35 0,35 0,33 0,35 0,18 0,18 0,16 0,16 0,35 0,30 0,30 0,30 0,30 0,74 0,74 0,46 0,28 0,28 0,35 0,63 0,78 0,78 0,41 0,41 0,78 1,70 0,41 0,41 0,25 0,25 0,78 0,45 0,30 0,11 0,13 0,12 0,13 0,13 0,13 0,78 0,78 0,78 0,93 0,50 0,50 0,70 0,49 0,70 0,46 0,69 0,69 0,78 1,02 1,02 1,02 0,65
KGO brutto 0,48 0,67 0,67 0,52 0,56 0,51 0,56 0,22 0,43 0,43 0,43 0,41 0,43 0,22 0,22 0,2 0,2 0,43 0,37 0,37 0,37 0,37 0,91 0,91 0,57 0,34 0,34 0,43 0,77 0,96 0,96 0,5 0,5 0,96 2,09 0,5 0,5 0,31 0,31 0,96 0,55 0,37 0,13 0,16 0,15 0,16 0,16 0,16 0,96 0,96 0,96 1,14 0,61 0,61 0,86 0,6 0,86 0,56 0,85 0,85 0,96 1,26 1,26 1,26 0,8
AR6290 ALL-in-ONE processor Steam food processor SCF 280 SCF 870/BY 20 BT 20EA BT 20W SBBC 203 BF 2051 Electronic Baby Scale with memory Animee 34Z015 AD 3131 AD 3133 AD 3134 AD 3136 AD 3137 S AD 3137 W Libra Silver 850/1 Libra DS 81 KS 05 Biay KS 05 Srebrny KS 22 KS 30 KS 32 KS 38 KS 40 KS 42 KS 45 KS 48 Cream KS 48 Flavour KS 48 Plain KS 50

Strona 265 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD AGD KATEGORIA Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne Wagi kuchenne MARKA BEURER BEURER BEURER BEURER BEURER BEURER BEURER BEURER BOMANN BOMANN BOSCH BRAVO CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CLATRONIC CONCEPT CONCEPT ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELDOM ELESKO ELESKO ELESKO ELESKO ELTA ELTA EXIDO FAGOR GORENJE GORENJE HOLDEN HOLDEN HOLDEN HOLDEN HOLDEN HOLDEN HYUNDAI HYUNDAI HYUNDAI IDEAL-AGD KENWOOD-AGD KENWOOD-AGD LAICA LAICA LAICA LAICA LAICA LAICA MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT PHILIPS PHILIPS NAZWA PRODUKTU KS 52 KS 55 KS 58 Biay KS 58 S KS 60 KS 61 KS 65 KS 70 KW 1411 CB KW 1413 CB MKW 0120 B 4118 KW 2582 KW 2943 KW 3051 KW 3052 KW 3367 EDS KWA 3115 VK-5510 VK-5520 DWK100 EK3052 Biay EK3052 Srebrny EK3130 EK4052 EK5055 Biay EK5055 Srebrny WK100 WK101 WK200S WK210 WK220 WKN100 WKN110 WKN120 WKN20S WKN25S WK-02 WK-03 WK-04 WK-05 KS 200 KS 250 HNS-017677 BC-200 KT 03NC KT 05NE WK-40 WK-41 WK-42 WK-50 WK-51 WK-52 KVE 301G KVE 301S KVE305 IWK100 DS 700 DS 800 BX 9260 BX 9310 KS 1006L KS 1006W KS 3002 KS 3009L EK-8013 EK-8013/B MWK-01 Biay MWK-01 Czarny MWK-01 Srebrny HR 2393/01 HR 2395/00

KGO netto 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55 0,55
KGO brutto 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68 0,68

Strona 335 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA FOTO FOTO FOTO FOTO FOTO FOTO FOTO KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KLIMATYZACJA KATEGORIA Kompakty Kompakty Kompakty Kompakty Kompakty Kompakty Kompakty Cyrkulatory Cyrkulatory Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki ceramiczne Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki halogenowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe Grzejniki konwektorowe MARKA SONY SONY SONY SONY SONY SONY SONY OPTIMUM OPTIMUM ADLER ARDES ARDES ELESKO HB HB PUR LINE PUR LINE RAVANSON ROWENTA ADLER ARDES ELESKO ELESKO ELESKO ELTA ELTA ELTA MPM PRODUCT OPTI OPTIMUM RAVANSON RAVANSON RAVANSON RAVANSON ADLER ARDES ARDES BOMANN CLATRONIC CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT CONCEPT ELDOM ELDOM ELDOM ELESKO ELESKO ELESKO ELTA FAGOR FAGOR HB HB HB HB HOLDEN HOLDEN MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT MPM PRODUCT NOVOTERM OPTIMUM OPTIMUM OPTIMUM OPTIMUM NAZWA PRODUKTU DSC-W85 DSC-W90/B DSC-W90/S DSC-WX1B DSC-WX1N DSC-WX1S NEX-5B WC-0350 WC-0400 AD 483 OC-1500 CFH 1801 E CTH 1801 R CHD 120 C Heaty 104 PTC-2007 Super SO 9005 AD OH-1200 OH-202 OK-9735 N1NFB 9735 N1W NSB-80Y4 OK-9300 GPQ-10 HH-1000T HH-1200 HH-1800 HH-2000 AD 462 KH 1055 CB KH 3077 KS-3000 KS-3001 KS-3002 KS-3003 KS-3004BC KS-3005 HC100 HC120T HC2000 K-100 K-101 K-AFL-60 TM AFL-61 T CH-2001 CH-2002T CH-2003S CH-2004ST CV-41 CV-42 CH-2004C MUG-02 MUG-03 MUG-05 MUG-06 N-16 YC-20F NW 300 A HNC-2000TT HNC-2002 HNC-2010 LX-2900

KGO netto 0,54 0,54 0,54 0,54 0,54 0,54 0,54 4,25 4,25 4,25 0,92 0,92 0,92 2,44 2,44 0,84 0,84 1,80 1,80 0,32 8,12 3,61 5,80 4,77 0,51 0,95 0,95 1,17 1,17 1,06 1,06 1,36 1,36 1,85 1,85 2,13 2,13 2,71 2,97 6,77 2,84 3,87 17,80 7,93 0,48 0,48 0,48 1,16 1,29 2,45 2,45 1,54 3,61 3,61 6,19 6,19 3,48 3,48 5,93 7,22 5,28 5,28 3,41 3,41 4,90 1,16 1,16 7,74 7,74 0,33 0,33 0,39
KGO brutto 0,67 0,67 0,67 0,67 0,67 0,67 0,67 5,23 5,23 5,23 1,13 1,13 1,3 1,03 1,03 2,22 2,22 0,39 9,99 4,44 7,14 5,87 0,63 1,17 1,17 1,44 1,44 1,3 1,3 1,67 1,67 2,28 2,28 2,62 2,62 3,33 3,65 8,33 3,49 4,76 21,89 9,75 0,59 0,59 0,59 1,43 1,59 3,01 3,01 1,9 4,44 4,44 7,61 7,61 4,28 4,28 7,3 8,88 6,5 6,5 4,2 4,2 6,03 1,43 1,43 9,52 9,52 0,4 0,4 0,48
SS-F6000 SS-MB250H/B SS-MB250H/M SS-MF450H/B SS-MF450H/M SS-X90ED/B SS-X90ED/M Platinum F Czarny Platinum F Orzech Platinum F Wenge Platinum S Czarny Platinum S Orzech Platinum S Wenge TPS-600F Heban TPS-600F Wenge Arena highline 500 S Czarny Arena highline 500 S Srebrny Arena highline 500 T Czarny Arena highline 500 T Srebrny Arena Satellite Canterbury SE Glenair 10 Glenair 15 Kensington SE Mini Autograph Revolution dc4 Espresso Revolution dc4 Jasny db Revolution dc4 t Espresso Revolution dc4 t Jasny db Revolution signature dc4 Espresso Revolution signature dc4 Jasny db Revolution signature dc4 t Espresso Revolution signature dc4 t Jasny db Revolution signature dc6 Espresso Revolution signature dc6 Jasny db Revolution signature dc6 t Espresso Revolution signature dc6 t Jasny db Sandringham SE Stirling SE TD12 TD8 Turnberry SE Westminster Royal SE Yorkminster SE LS-H255 Czarny LS-H255 Klon LS-H255 Orzech NS-125F NS-225F NS-325F Czarny NS-325F Winia NS-333 NS-50 Czarny NS-50 Winia NS-515F Czarny NS-515F Winia NS-525F Czarny NS-525F Winia NS-555 NS-777 NS-7900 NS-7900 Czarny NS-8900 Orzech NS-8900 Winia NS-8HX NS-B700 Brzowy NS-B700 Czarny NS-F700 Brzowy NS-F700 Czarny NS-M125 Czarny NS-M125 Srebrny NS-M225 Brzoza

Strona 368 z 457

Havo Sp. z o.o. ul. Przdzalniana 6H 15-688 Biaystok 542-24-71-782 BRANA RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV RTV KATEGORIA Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny gonikowe Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Kolumny cienne Magnetofony Magnetofony Magnetofony Magnetofony Magnetofony Magnetofony Magnetofony Magnetofony Magnetofony osobiste Magnetofony osobiste Magnetofony osobiste Magnetofony osobiste Magnetowidy Magnetowidy Magnetowidy Magnetowidy Magnetowidy Magnetowidy Magnetowidy Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD Magnetowidy z DVD MD przenone MD przenone Mikrofony Mikrofony Mikrofony Mikrofony Mikrofony Mikrofony MARKA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA YAMAHA MAGNAT MAGNAT MAGNAT MAGNAT MAGNAT TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA TAGA VELODYNE VELODYNE ION AUDIO ONKYO ONKYO PIONEER SONY SONY TEAC TEAC SONY SONY SONY SONY PANASONIC PANASONIC PANASONIC SHARP SHARP SONY SONY LG PANASONIC PANASONIC PHILIPS SAMSUNG SHARP SHARP SONY SONY SONY PHILIPS PHILIPS PHILIPS PIONEER PIONEER SONY NAZWA PRODUKTU NS-M225 Czarny NS-M325 Czarny NS-M325 Winia NS-M515 NS-M525 Czarny NS-M525 Winia Soavo-900M Soavo-900M Brzowa brzoza Soavo-900M Ciemny brz Soavo-900M Naturalna brzoza Interior IC 62 Interior IC 82 Interior IW 510C Interior IW 610 Interior IW 810 GTCS-50 GTCS-65 TCP-200 TCW-45RV TCW-46RV TCW-500R v.3 TCW-550R TCW-55V TCW-64RV TCW-66RV TCW-72V TCW-750 TCW-86RV TLCR-400 TLCR-525 TOS-415B TOS-415W TOS-715B TOS-715W TOW-65V SC-IF/IC SC-IW Tape 2 PC TA-RW255 Czarny TA-RW255 Srebrny CT-W208R TC-WE475/B TC-WE475/S W-600R W-865R WM-EX190 WM-EX362 WM-FX383 WM-GX410 NV-HV51EP/S NV-HV61EP/K NV-HV61EP/S VC-FH300BM VC-MH780BM SLV-SE640N SLV-SE840N V280 NV-VP23 NV-VP33 DVP3350V DVD-V6800 DV-NC200S DV-NC230S SLV-D970PN MZ-RH10 MZ-RH910 SBC MC 8650 SBC MD 110 SBC MD 150 DM-DV05 DM-DV15 ECM-719

KGO netto 1,98 1,98 1,59 1,59 1,49 1,49 1,49 3,11 3,11 1,59 1,59 3,63 3,63 3,63 4,46 4,46 7,22 7,22 5,57 5,57 8,46 1,30 6,45 25,30 6,91 6,91 9,94 9,94 13,93 13,93 12,19 12,19 17,86 17,86 6,45 6,45 6,45 6,02 6,02 7,02 7,02 7,13 7,13 8,76 8,76 8,34 8,34 8,89 8,89 6,11 6,11 3,24 3,24 2,40 2,40 2,40 5,03 5,03 5,03 5,27 5,27 5,27 4,44 4,44 4,44 4,17 4,17 4,17 4,17
KGO brutto 2,44 2,44 1,95 1,95 1,83 1,83 1,83 3,82 3,82 1,96 1,96 4,47 4,47 4,47 5,49 5,49 8,88 8,88 6,85 6,85 10,4 1,6 7,93 31,12 8,5 8,5 12,23 12,23 17,14 17,14 14,99 14,99 21,97 21,97 7,93 7,93 7,93 7,41 7,41 8,63 8,63 8,77 8,77 10,78 10,78 10,26 10,26 10,93 10,93 7,51 7,51 3,98 3,98 2,95 2,95 2,95 6,19 6,19 6,19 6,48 6,48 6,48 5,46 5,46 5,46 5,13 5,13 5,13 5,13
Movie 155 QX Biay wysoki poysk Movie 155 QX Czarny wysoki poysk Movie 65 CX Czarny Movie 65 CX Srebrny Movie 85 CX Biay wysoki poysk Movie 85 CX Czarny wysoki poysk Movie 85 CX Srebrny wysoki poysk Movie CD 1000.2 Czarny Movie CD 1000.2 Srebrny Movie CD 105 Czarny Movie CD 105 Srebrny Movie CD 2000.2 Biay byszczcy Movie CD 2000.2 Czarny byszczcy Movie CD 2000.2 Srebrne aluminium Universe Universe Calvados 500 Set Czarny 500 Set Winia C30050c50 Czarny C30050c50 Winia RC-50 Set Winia SP-EXA3 HD Theater 500 PALLADIUM Set REFERENCE 52 HT Czarny REFERENCE 52 HT Winia REFERENCE 62 HT Czarny REFERENCE 62 HT Winia REFERENCE 63 HT Czarny REFERENCE 63 HT Winia REFERENCE 82 HTB Czarny REFERENCE 82 HTB Winia REFERENCE 83 Set Czarny REFERENCE 83 Set Winia RF-63 (RF63-RC64-RS62) SYNERGY F1-C1-B2 HT-B Czarny SYNERGY F1-C1-B2 HT-B Winia SYNERGY F1-C1-S1 Czarny SYNERGY F1-C1-S1 HT SYNERGY F2-C1-B2 Czarny SYNERGY F2-C1-B2 Winia SYNERGY F2-C2-B2 HTB Czarny SYNERGY F2-C2-B2 HTB Winia SYNERGY F3-C1-B2 Czarny SYNERGY F3-C1-B2 Winia SYNERGY F3-C1-S2 Czarny SYNERGY F3-C1-S2 Winia SYNERGY F3-C2-B2 Czarny SYNERGY F3-C2-B2 Winia AV-702 v.3 ciemny orzech AV-702 v.3 czarny AV-707 v.3 + SW-850 v.2 Ciemny orzech AV-707 v.3 + SW-850 v.2 Czarny AV-707 v.3 Ciemny orzech AV-707 v.3 Czarny AV-707 v.3 Orzech rany F-51v.3 F/S/C + SW-550v.2 Ciemny orzech F-51v.3 F/S/C + SW-550v.2 Czarny F-51v.3 F/S/C + SW-550v.2 Grusza F-51v.3 F/S/C + SW-850v.2 Ciemny orzech F-51v.3 F/S/C + SW-850v.2 Czarny F-51v.3 F/S/C + SW-850v.2 Grusza F-51v.3 F/S/C Ciemny orzech F-51v.3 F/S/C Czarny F-51v.3 F/S/C Grusza K-5300FCS + SW5300 calvados K-5300FCS + SW5300 ciemny orzech K-5300FCS + SW5300 grusza K-5300FCS + SW5300 heban

Francesca M. Pisani Istituto di Biochimica delle Proteine, Consiglio Nazionale Ricerche, 80131 Napoli, Italy, fm.pisani@ibp.cnr.it Helmut Pospiech Leibniz Institute for Age Research Fritz Lipmann Institute, D-07745 Jena, Germany ine M. Prendergast Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland Lisa Prendergast Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland Nadine Quinn Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland Stephen Rea Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland, stephen.rea@nuigalway.ie N.I. Rechkunova Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia Kristina Rehmet Cell Cycle Control Laboratory, School of Natural Sciences, Centre of Chromosome Biology, National University of Ireland Galway, Galway, Ireland A. Ivana Scovassi Istituto di Genetica Molecolare CNR, 27100 Pavia, Italy, scovassi@igm.cnr.it Noriko Shimazaki USC Norris Comprehensive Cancer Center, Los Angeles, CA 90089-9176, USA Parkkinen Sinikka Faculty of Biosciences, University of Joensuu, FI-80101 Joensuu, Finland Anna M. Sokol DNA Damage Response Laboratory, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland Kevin F. Sullivan Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland, kevin.sullivan@nuigalway.ie Juhani E. Syvoja Faculty of Biosciences, University of Joensuu, FI-80101 Joensuu, Finland, juhani.syvaoja@joensuu. Albert G. Tsai USC Norris Comprehensive Cancer Center, Los Angeles, CA 90089-9176, USA Sangamitra Villalan DNA Damage Response Laboratory, Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland Lisa Wiesmller Department of Obstetrics and Gynaecology, University of Ulm, D-89075 Ulm, Germany, lisa.wiesmueller@uni-ulm.de

HP1 HP1

Sensing and binding of DSB by the MRN complex
Phosphorylation and release of HP1 bound to H3K79me Phosphorylation and release of linker H1 Acetylation of H3/H4 by TIP60 De-phosphorylation of H2AX tyrosine 142 by EYA

P P HP1 P

Nucleosome remodelling through incorporation of H2AX

ATM P P

Acetylation of H4K16 by MOF Phosphorylation of H2AX by ATM
Recruitment of MDC1 to H2AX Phosphorylation of MDC1

P MDC1 P

Recruitment of RNF8 and local ubiquitylation of H2A + H2AX

RNF8 RNF168

RNF168
BRCA1 binds to ubiquitin via RNF8 53BP1 binds cooperatively to H3K79me/H4K20me and H2AX

RAP80 ABRA1 BRCA1

53BP1 M M P

P RAP80 ABRA1 BRCA1

P MDC1

RNF168 P

Checkpoint activation

Physical repair of DSB

Fig. 2 Model showing stages of the DDR and associated modications
Removal of repair proteins
Repositioning of nucleosome Removal of modifications Active remodelling by TIP60 acetylation of H2AX-K5 and UBC13 ubiquitylating K119

TIP60 A

CAF-1 deposition of H3K56 acetylated nucleosomes Re-loading of H1 linkers
Chromatin condensation by binding of HP1 to H3K9me Phosphorylation of H2AX tyrosine 142 by WSTF kinase

P WSTF

Fig. 2 (continued)
in impaired phosphorylation of S139 of H2AX and impaired recruitment of DNA damage response proteins (Cook et al., 2009; Xiao et al., 2009). This suggests that although Y142 is de-phosphorylated following IR some basal level of phosphorylation may be needed to promote or maintain S139 phosphorylation and the DNA damage repair processes.

Methylation of H3K79

Methylation of various lysine residues on histones H3 and H4 has also been suggested to play a role in the early events of the DNA damage response (Huyen et al., 2004; Kouzarides, 2007). 53BP1 is a conserved DDR protein that rapidly localises to DSBs following DNA damage (Anderson et al., 2001; Schultz et al., 2000) and contributes to the phosphorylation and activation of the Chk2 effector kinase (Wilson and Stern, 2008). 53BP1 possesses a tandem tudor domain that mediates its in vitro binding to histone H3 methylated at lysine 79 (H3K79me) and which is required for its targeting to DSBs (Huyen et al., 2004). Furthermore, depletion of DOT1L, the methyltransferase responsible for H3K79me, inhibits the recruitment of 53BP1 to DSBs, providing further evidence that this modication is important for the recognition of breaks by 53BP1. Curiously, no detectable change has been observed for
overall H3K79 methylation levels in response to damage by irradiation. It has therefore been suggested that 53BP1 can sense DSBs via changes in chromatin structure that expose a previously concealed H3K79me binding platform (Huyen et al., 2004; Fig. 2b).

Histone Acetylation in the DDR
Along with modications including phosphorylation, methylation and ubiquitylation, acetylation of histones has been shown to play an important role in the DDR (Table 1). Acetylation of conserved lysine residues on the tails of H3 and H4 is important for normal cell growth and an efcient DDR following treatment with damaging agents such as MMS or breaks induced by the endonuclease EcoR1 (Bird et al., 2002; Choy and Kron, 2002; Qin and Parthun, 2002). Similarly, mutation of the histone acetyltransferases (HATs) responsible for these acetylation events, NuA4, Gcn5 and Hat1, also confers sensitivity to DSB-inducing agents (Downs
T. Costelloe and N.F. Lowndes Table 1 Histone modications involved in the DNA damage response

Histone residue H2AS129

Modication Phosphorylation

Enzyme Mec1, Tel1

Function Stable retention of DDR checkpoint proteins at DSB, DSB repair Rad9 recruitment to DSBs, checkpoint activation. Marks euchromatin
References Downs et al. (2007)

Methylation

H2BK123 H3K56
Ubiquitylation Acetylation

Rad6-Bre1 Rtt109

H3K9, K14, K18, K23, K27

Acetylation

Hat1, Gcn5

H4K5, K8, K12, K16

Giannattasio et al. (2005), Grenon et al. (2007), Huyen et al. (2004), van Leeuwen et al. (2002), Wysocki et al. (2005) Checkpoint activation. Giannattasio et al. H3K4me1 localised (2005), Wysocki to silenced et al. (2005) chromatin, H3K4me2, H3K4me3 mark 5 region of active genes Checkpoint activation Giannattasio et al. (2005) Modication regulated Chen et al. (2008), Driscoll et al. in cell cycle and (2007), Han et al. DNA damage (2007), Hyland dependent manner. et al. (2005), Li Required for et al. (2008) chromatin assembly following DNA replication and DNA repair. H3K56ac signals checkpoint recovery following DNA damage Li et al. (2008), Qin Acetylation of and Parthun N-terminal lysines (2002) of H3 required for efcient DDR following MMS treatment Acetylation of Bird et al. (2002), N-terminal lysines Choy and Kron of H4 plays minor (2002) roles in DDR following MMS or CPT treatment
et al., 2004; Qin and Parthun, 2002). In mammalian cells, the Tip60 HAT is also recruited to sites of DSBs, and is required for acetylation of H4 and efcient homologous recombination (HR) (Murr et al., 2006). Similarly, hMOF is required for DNA damage-induced acetylation of H4K16, and defective acetylation of H3 and H4 has been linked to defective cellular responses to DNA damage (see Chubb and Rea, and references therein). Finally, H2A acetylation has also been implicated in the DDR, an event carried out by the HAT NuA4, and shown to be required for efcient repair of DNA damage (Bird et al., 2002; Moore et al., 2007).

H2AX Protein

Despite the large amount of attention paid to the DNA damage-linked serine phosphorylation by PIKKs, the H2AX protein itself has a number of additional unique properties. The dening feature of H2AX is considered to be the C-terminal region with the SQ[E/D] motif (Fig. 3). As mentioned in section Denition of H2AX, the number of residues separating this motif from the histone fold is variable and claimed to correlate with the evolutionary complexity of the organism (Redon et al., 2002). The residues responsible for this variable spacing are mainly hydrophilic with a high glycine and proline content suggesting a exible, unstructured nature so the basis for the correlation could be more directly related to a structural constraint such as the
62 Fig. 3 Sequence logo of all human canonical H2A isoforms showing differences with H2AX below. The 4 residues changes from H2A to H2AX outside the C-terminal region are Gln6Thr, Thr16Ser, Asn38His and Lys99Gly. Alignment of H2A genes was made usign edialign (Morgenstern, 1999) from EMBOSS (Rice et al., 2000) and WebLogo 3 (Crooks et al., 2004).
variation in internucleosomal repeat lengths of organisms which itself shows linkage with evolutionary complexity. In addition to the C-terminal motif, amino acid residues 6, 16, 38 and 99 of H2AX are different from the human H2A.1 consensus (Figs. 3 and 4). Inspection of the human and X. laevis histone based nucleosome structures reveals that H2A
Fig. 4 Nucleosome structure highlighting differences between H2A and H2AX. H2A chain is highlighted and white frames indicate the position of the residues that differ between the human canonical H2A and H2AX. Image from PDB structure 1KX5 using PyMOL (DeLano, 2002).
residue 6 is located in the exible N-terminal tail and residue 16 is located at the very base of the tail (Figs. 4 and 5b) which tracks the minor groove at superhelical location 4.5 (SHL4.5) (Fig. 5a). The substitution of glutamine with threonine at position 6 in H2AX introduces a potential hydroxyl site for post-translational modication that is not present for the glutamine in canonical H2A. In contrast, the threonine to serine substitution conserves the modiable hydroxyl at position 16. Asparagine 38 is located in the loop between the 1 and 2 helices of H2A within the nucleosome (Figs. 4 and 5c). Importantly, this residue makes direct contact with the equivalent amino acid in the other H2AH2B dimer in the nucleosome structure and has been suggested to affect both nucleosome stability and the balance between homotypic and heterotypic combinations (see section H2AX Distribution in Chromatin) of H2A types within the yeast nucleosome (White et al., 2001). It is possible that the change of residue 38 from asparagine in H2A to histidine in H2AX could also affect nucleosome stability and dynamics. For example, weakening of interactions between the two H2AH2B histone fold dimers could result in increased nucleosome exing and impact the ability to condense into stable higher order chromatin structure. Furthermore, the presence of the histidine in H2AX could affect the stabilisation of a second copy of H2AX relative to canonical H2A within the nucleosome. This change of asparagine to histidine at position 38 occurs only in higher organism H2AX and could potentially drive a bias towards either homotypic H2AX-only or heterotypic H2AXH2A mixed nucleosomes which could

A Phosphorylation Switch for the Initiation of DNA Replication
Recent experiments have shown that S-CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast (Zegerman and Difey, 2007; Tanaka et al., 2007b). Dpb11 forms a ternary complex with the replication initiation
factors Sld2 and Sld3 when these become phosphorylated (Fig. 1). This complex then controls the association of Cdc45 and the replicative DNA polymerases with the origins of DNA replication (Masumoto et al., 2000). Tanaka and co-workers (Tanaka et al., 2007b) were able to demonstrate that a phospho-mimetic form of Sld2 (Sld2-11D) confers S-CDK-independent DNA replication when combined with either the JET1 mutation of Cdc45, or overexpression of Dpb11. Both JET1 and Dpb11 over-expression overcomes the requirement for Sld3 phosphorylation for initiation of DNA replication. Zegerman and Difey (2007) fused an Sld3 mutant that cannot become phosphorylated by S-CDK and that is decient for DNA replication with the sequence for the amino-terminal BRCT domain pair of Dpb11. In a strain where S-CDK activity was inhibited at the same time, almost no DNA replication occurs. But when wild-type SLD2 is in addition replaced by the phospho-mimetic SLD2-T84D variant, extensive DNA replication occurs, bypassing the requirement for S-CDK activity. Therefore, the phosphorylation of Sld2 and Sld3, and their subsequent binding by Dpb11 represents the minimal requirement for CDK-dependent activation of replication initiation in yeast (Tanaka et al., 2007a). The subsequent recruitment of Cdc45 into this complex (via Sld3) might be the rate-limiting step for the formation of an active replicative DNA helicase, i.e. the Cdc45MCM2-7-GINS complex (Moyer et al., 2006; Boskovic et al., 2007; Aparicio et al., 2009). As discussed above, the corresponding regulatory mechanisms in higher eukaryotes are poorly understood. Considering the roles of TopBP1/XCut5, RecQL4 and Cdc45 for the loading of the replicative DNA polymerases and establishment of the replication fork both in Xenopus and in human cells, a similar regulatory network as in yeast can be assumed for vertebrates. The targets for S-CDK and DDK among these proteins have not yet been identied. What is more, it remains unclear, which vertebrate factor takes over the role of yeast Sld3. Since human Cdc45 interacts directly with TopBP1, it is conceivable that human TopBP1 abrogates a requirement of Sld3. But in yeast, DDK-dependent loading of Sld3 appears to be the most upstream event in the initiation cascade, and a comparable regulatory step is not yet in sight in higher eukaryotes.
GINS: An Evolutionarily Conserved Key Player in DNA Replication Identication of the GINS Complex

Kim, J. E., McAvoy, S. A., Smith, D. I., and Chen, J. (2005) Human TopBP1 ensures genome integrity during normal S phase. Mol Cell Biol, 25, 1090710915. Kramer, A., Lukas, J., and Bartek, J. (2004) Checking out the centrosome. Cell Cycle, 3, 13901393. Kubota, Y., Takase, Y., Komori, Y., Hashimoto, Y., Arata, T., Kamimura, Y., Araki, H., and Takisawa, H. (2003) A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication. Genes Dev, 17, 11411152. Kumagai, A., Lee, J., Yoo, H. Y., and Dunphy, W. G. (2006) TopBP1 activates the ATR-ATRIP complex. Cell, 124, 943955. Lee, J., Kumagai, A., and Dunphy, W. G. (2007) The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR. J Biol Chem, 282, 2803628044. Liu, K., Lin, F. T., Ruppert, J. M., and Lin, W. C. (2003) Regulation of E2F1 by BRCT domaincontaining protein TopBP1. Mol Cell Biol, 23, 32873304. Liu, K., Luo, Y., Lin, F. T., and Lin, W. C. (2004) TopBP1 recruits Brg1/Brm to repress E2F1induced apoptosis, a novel pRb-independent and E2F1-specic control for cell survival. Genes Dev, 18, 673686. Liu, K., Paik, J. C., Wang, B., Lin, F. T., and Lin, W. C. (2006) Regulation of TopBP1 oligomerization by Akt/PKB for cell survival. EMBO J, 25, 47954807. Lou, Z., Minter-Dykhouse, K., Franco, S., Gostissa, M., Rivera, M. A., Celeste, A., Manis, J. P., van Deursen, J., Nussenzweig, A., Paull, T. T., Alt, F. W., and Chen, J. (2006) MDC1 maintains genomic stability by participating in the amplication of ATM-dependent DNA damage signals. Mol Cell, 21, 187200. Lupardus, P. J. and Cimprich, K. A. (2006) Phosphorylation of Xenopus Rad1 and Hus1 denes a readout for ATR activation that is independent of Claspin and the Rad9 carboxy terminus. Mol Biol Cell, 17, 15591569. Makiniemi, M., Hillukkala, T., Tuusa, J., Reini, K., Vaara, M., Huang, D., Pospiech, H., Majuri, I., Westerling, T., Makela, T. P., and Syvaoja, J. E. (2001) BRCT domain-containing protein TopBP1 functions in DNA replication and damage response. J Biol Chem, 276, 3039930406. Manke, I. A., Lowery, D. M., Nguyen, A., and Yaffe, M. B. (2003) BRCT repeats as phosphopeptide-binding modules involved in protein targeting. Science, 302, 636639. Masumoto, H., Sugino, A., and Araki, H. (2000) Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast. Mol Cell Biol, 20, 28092817. Matsuno, K., Kumano, M., Kubota, Y., Hashimoto, Y., and Takisawa, H. (2006) The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase alpha in the initiation of DNA replication. Mol Cell Biol, 26, 48434852. Mordes, D. A., Glick, G. G., Zhao, R., and Cortez, D. (2008) TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev, 22, 14781489. Morishima, K., Sakamoto, S., Kobayashi, J., Izumi, H., Suda, T., Matsumoto, Y., Tauchi, H., Ide, H., Komatsu, K., and Matsuura, S. (2007) TopBP1 associates with NBS1 and is involved in homologous recombination repair. Biochem Biophys Res Commun, 362, 872879. Morris, J. S., Nixon, C., Bruck, A., Nasir, L., Morgan, I. M., and Philbey, A. W. (2008) Immunohistochemical expression of TopBP1 in feline mammary neoplasia in relation to histological grade, Ki67, ER and p53. Vet J, 175, 218226. Morris, J. S., Nixon, C., King, O. J., Morgan, I. M., and Philbey, A. W. (2009) Expression of TopBP1 in canine mammary neoplasia in relation to histological type, Ki67, ER and p53. Vet J, 179, 422429. Nasheuer, H. P., Pospiech, H., and Syvoja, J. (2007) Progress towards the anatomy of the eukaryotic DNA replication fork. In: Lankenau, D. H. (Ed.) Genome Integrity: Facets and Perspectives, Genome Dynamics & Stability, Vol. 1, Springer, Berlin-Heidelberg-NewYork, pp. 2768. Nasheuer, H. P., Smith, R., Bauerschmidt, C., Grosse, F., and Weisshart, K. (2002) Initiation of eukaryotic DNA replication: regulation and mechanisms. Prog Nucleic Acid Res Mol Biol, 72, 4194.

cycle and apoptosis. Chk1 inhibits CDC25 phosphatases by causing their nuclear exclusion or degradation, which in turn prompts the accumulation of inhibitory phosphorylation of CDKs. Chk2 and ATM signal collaborated with p53 to induce different pro-.apoptotic factors (like Puma, Bax, Noxa), oxidative-stress response genes and DNA repair, the expression of p21Cip1/WAF1 and the feedback regulator HDM2 (the human orthologue of the mouse double minute 2, MDM2). In cancer cells, which are often defective in ATR and ATM pathways, unscheduled HR might lead to high rates of chromosome breakage and illegitimate fusions, contribution to genomic instability. Non-homologous end joining (NHEJ). For efcient rejoining of broken chromosome ends by NHEJ, a number of specic factors are needed, including the complex formed by of XRCC4 and DNA ligase IV, a DNA end binding heterodimer, Ku70 and Ku80, DNA-PK and the recently described accessory factor XLF/Cernunnos. All these factors are essential for the NHEJ reaction and the absence of only one of them can induce sensitivity to double-strand break inducing agents and premature cellular senescence (Mills et al., 2003; Sekiguchi and Ferguson, 2006). NHEJ relies on short homologous sequences called microhomologies present on the singlestranded tails of the DNA ends to be joined. If the structure of broken ends do not show microhomologies, NHEJ employs different factors to process the ends, including the family X DNA polymerases Terminal deoxynucleotidyl transferase (TdT), Pol and Pol (Nick McElhinny and Ramsden, 2004), to extend the terminus of the broken ends to create such regions. Another important role played by NHEJ is joining hairpin-capped double-strand breaks induced during V(D)J recombination. Such a process generates diversity in B-cell and T-cell receptors in the vertebrate immune system (Budman and Chu, 2005) (see also Nonhomologous DNA End Joining (NHEJ) and Chromosomal Translocations in Humans by Lieber et al., this book). However, NHEJ is intrinsically an error-prone process. Thus, high frequencies of DSBs when repaired by NHEJ, can substantially increase the spontaneous mutagenesis rate, accelerating tumor progression. Translesion synthesis (TLS). In mammalian cells, during DNA replication, there are situations where a lesion in DNA can result in replication fork stalling due to inability of replicative Pols to bypass lesions like abasic (AP) sites or bulky DNA template adducts, thymine-thymine or cyclobutane pyrimidine dimers and cis-platinum adducts (Prakash et al., 2005; Waters et al., 2009). In recent years a variety of so-called translesion Pols have been identied. These enzymes exhibit a distributive DNA synthesis over the lesions, lack the proofreading activity and are usually error-prone in copying canonical DNA templates. Their ability to incorporate nucleotide opposite bulky modied bases is due to a wider nucleotide-binding pocket with respect to the replicative enzymes. Since TLS Pols perform DNA synthesis often in an error-prone manner, this can lead to accumulation of mutations in the newly synthesized DNA. According to the so-called mutagenesis hypothesis of carcinogenesis, mutations are a prerequisite for most, if not all, cancers. Since error-prone TLS constitutes a major mechanism of mutagenesis, its deregulated activity can substantially contribute to the mutated phenotype of cancer cells. Indeed, many if not all the TLS enzymes are often found overexpressed in cancer

Concluding Remarks

A cancer cell feature is genome instability resulting in chromosome breaks and generation of fusions between oncogenes and cellular genes, inactivation of tumor suppressor genes and drug resistance genes amplication. A state of genomic instability can be achieved through the loss of DNA damage signaling and checkpoint pathways or by defects in one or more of the six major DNA repair pathways. For these reasons, understanding how the enzymes implicated in the different DNA repair pathways are regulated in normal and in tumor cells will provide signicant novel information on cancer outset. The identication of key proteins involved in these pathways might also provide novel markers for cancer diagnosis and new avenues for the development of antiproliferative (anticancer) drugs. As mentioned above, cancer cell are usually defective for at least one DNA repair pathway. The specic pathway affected is predictive of the kinds of mutations so, under this respect, the mechanisms through which tumor cells respond to and deal with damaged DNA have profound implications in the development of the cancer phenotype. Moreover, the specic DNA repair pathways altered in cancer cells can cause specic tumor drugs sensitivity and could help in the choice of optimal treatment.
Acknowledgments Work in authors laboratories has been partially supported by an AIRCInvestigator Grant to GM. EC is the recipient of a FIRC Fellowship
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DNA Polymerase , a Key Protein in Translesion Synthesis in Human Cells
Sverine Cruet-Hennequart, Kathleen Gallagher, Anna M. Sokl, Sangamitra Villalan, ine M. Prendergast, and Michael P. Carty
Abstract Genomic DNA is constantly damaged by exposure to exogenous and endogenous agents. Bulky adducts such as UV-induced cyclobutane pyrimidine dimers (CPDs) in the template DNA present a barrier to DNA synthesis by the major eukaryotic replicative polymerases including DNA polymerase. Translesion synthesis (TLS) carried out by specialized DNA polymerases is an evolutionarily conserved mechanism of DNA damage tolerance. The Y family of DNA polymerases, including DNA polymerase (Pol ), the subject of this chapter, play a key role in TLS. Mutations in the human POLH gene encoding Pol underlie the genetic disease xeroderma pigmentosum variant (XPV), characterized by sun sensitivity, elevated incidence of skin cancer, and at the cellular level, by delayed replication and hypermutability after UV-irradiation. Pol is a low delity enzyme when copying undamaged DNA, but can carry out error-free TLS at sites of UVinduced dithymine CPDs. The active site of Pol has an open conformation that can accommodate CPDs, as well as cisplatin-induced intrastrand DNA crosslinks. Pol is recruited to sites of replication arrest in a tightly regulated process through interaction with PCNA. Pol -decient cells show strong activation of downstream DNA damage responses including ATR signaling, and accumulate strand breaks as a result of replication fork collapse. Thus, Pol plays an important role in preventing genome instability after UV- and cisplatin-induced DNA damage. Inhibition of DNA damage tolerance pathways in tumors might also represent an approach to potentiate the effects of DNA damaging agents such as cisplatin. Keywords DNA polymerase eta Translesion synthesis XPV UV Cisplatin DDR

Haracska, L., Johnson, R. E., Unk, I., Phillips, B., Hurwitz, J., Prakash, L., and Prakash, S. (2001a) Physical and functional interactions of human DNA polymerase eta with PCNA. Mol Cell Biol, 21, 71997206. Haracska, L., Johnson, R. E., Unk, I., Phillips, B. B., Hurwitz, J., Prakash, L., and Prakash, S. (2001b) Targeting of human DNA polymerase iota to the replication machinery via interaction with PCNA. Proc Natl Acad Sci USA, 98, 1425614261. Haracska, L., Kondratick, C. M., Unk, I., Prakash, S., and Prakash, L. (2001c) Interaction with PCNA is essential for yeast DNA polymerase eta function. Mol Cell, 8, 407415. Haracska, L., Prakash, S., and Prakash, L. (2000a) Replication past O(6)-methylguanine by yeast and human DNA polymerase eta. Mol Cell Biol, 20, 80018007. Haracska, L., Torres-Ramos, C. A., Johnson, R. E., Prakash, S., and Prakash, L. (2004) Opposing effects of ubiquitin conjugation and SUMO modication of PCNA on replicational bypass of DNA lesions in Saccharomyces cerevisiae. Mol Cell Biol, 24, 42674274. Haracska, L., Unk, I., Johnson, R. E., Phillips, B. B., Hurwitz, J., Prakash, L., and Prakash, S. (2002) Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol Cell Biol, 22, 784791. Haracska, L., Washington, M. T., Prakash, S., and Prakash, L. (2001d) Inefcient bypass of an abasic site by DNA polymerase eta. J Biol Chem, 276, 68616866. Haracska, L., Yu, S. L., Johnson, R. E., Prakash, L., and Prakash, S. (2000b) Efcient and accurate replication in the presence of 7,8-dihydro-8-oxoguanine by DNA polymerase eta. Nat Genet, 25, 458461. Harper, J. W. and Elledge, S. J. (2007) The DNA damage response: ten years after. Mol Cell, 28, 739745. Hendel, A., Ziv, O., Gueranger, Q., Geacintov, N., and Livneh, Z. (2008) Reduced efciency and increased mutagenicity of translesion DNA synthesis across a TT cyclobutane pyrimidine dimer, but not a TT 6-4 photoproduct, in human cells lacking DNA polymerase eta. DNA Repair, 7, 16361646. Hishida, T., Kubota, Y., Carr, A. M., and Iwasaki, H. (2009) RAD6-RAD18-RAD5-pathwaydependent tolerance to chronic low-dose ultraviolet light. Nature, 457, 612615. Hoege, C., Pfander, B., Moldovan, G. L., Pyrowolakis, G., and Jentsch, S. (2002) RAD6-dependent DNA repair is linked to modication of PCNA by ubiquitin and SUMO. Nature, 419, 135141. Huang, T. T., Nijman, S. M. B., Mirchandani, K. D., Galardy, P. J., Cohn, M. A., Haas, W., Gygi, S. P., Ploegh, H. L., Bernards, R., and DAndrea, A. D. (2006) Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol, 8, 341347. Indiani, C., McInerney, P., Georgescu, R., Goodman, M. F., and ODonnell, M. (2005) A slidingclamp toolbelt binds high- and low-delity DNA polymerases simultaneously. Mol Cell, 19, 805815. Johnson, R. E., Haracska, L., Prakash, S., and Prakash, L. (2001) Role of DNA polymerase zeta in the bypass of a (6-4) TT photoproduct. Mol Cell Biol, 21, 35583563. Johnson, R. E., Kondratick, C. M., Prakash, S., and Prakash, L. (1999a) hRAD30 mutations in the variant form of xeroderma pigmentosum. Science, 285, 263265. Johnson, R. E., Prakash, S., and Prakash, L. (1999b) Efcient bypass of a thymine-thymine dimer by yeast DNA polymerase, Pol-eta. Science, 283, 10011004. Johnson, R. E., Trincao, J., Aggarwal, A. K., Prakash, S., and Prakash, L. (2003) Deoxynucleotide triphosphate binding mode conserved in Y family DNA polymerases. Mol Cell Biol, 23, 30083012. Kannouche, P., Broughton, B. C., Volker, M., Hanaoka, F., Mullenders, L. H., and Lehmann, A. R. (2001) Domain structure, localization, and function of DNA polymerase eta, defective in xeroderma pigmentosum variant cells. Genes Dev, 15, 158172. Kannouche, P., Fernandez de Henestrosa, A. R., Coull, B., Vidal, A. E., Gray, C., Zicha, D., Woodgate, R., and Lehmann, A. R. (2003) Localization of DNA polymerases eta and iota to the replication machinery is tightly co-ordinated in human cells. EMBO J, 22, 12231233.

Human POLG gene

E1143G* E1143G* 3482+2(T>C)splice M1163R L1173fs X K1191N K1191R

Y1210fs1216X

R1096H Q879H R1096C T885S L886P R1047W G888S T914P W1020X R943C A957P R964C L965X L966R A B

Fingers

H277C L304R L304R E873X G517V K561M A767D T251I A862T S305R Q497H H569Q G763R L244P R853Q R309H A467T F749S R574W W235X R852C T326fs387X A467T P587L P648R W748S R232G T851A L428P R232H P589L W748S L83P T849X W347fs356X R227P L605R G737R G11D Q68X R227W G848S R374X R627W G11D 2157 splice 2480 splice C224Y R627Q C418R A143V Mitochondria R807C Q715X R417T R627Q targeting sequence I II III C
Palm Thumb Spacer Thumb Palm

Exonuclease

R807P L424X M430L G431V R597W R709X G746S G763R 2354Gins P587L R579W R574W A467T R562Q S511N N468D S433C T452X L463F A847X G848S R853W A862T R617C M603L P648R Y831C*

F961S A957S

G1205A D1196N

(CAG)n

Y955C R953C R1047W R1047Q R943H G1051R G1076V H932Y N864S V855A A889T G923D W918R

W312R G380D

D1184N R1187C S1176L F1164I R1138C I1079L R1128H V1106I S1095R A1105T R1096C S1104C
Fig. 1 Schematic diagram of human DNA polymerase protein showing the location of amino acid substitutions resulting from disease and polymorphism mutations. Disease substitutions above the line that are not Boxed are associated with Alpers and myocerebralhepatopathy syndrome, while Boxed substations above the line are associated with ataxia-neuropathy syndromes. Mutations below the line are associated with various forms of progressive external ophthalmoplegia where Boxed mutations are autosomal dominant PEO substitutions. The (CAG)n repeat is associated with male infertility or idiopathic Parkinsons disease. Arrows and substitutions with an asteric depict the non-synonymous polymorphic amino acid changes
(SNP), which is found in 4% of European populations. The W748S mutations has intrinsic lower polymerase activity as well as a demonstrated lower afnity for DNA (Chan et al., 2006). We have found that the E1143G SNP can modulate the deleterious effect of the W748S mutation (Chan et al., 2006). This nding raises the possibility that other SNPs could potentially affect POLG enzymatic activity. Four adPEO mutations, G923D, R943H, Y955C and A957S that are found in and around motif B in the active site of the DNA polymerase were characterized biochemically (Graziewicz et al., 2004). Two of the substitutions, R943H and Y955C, change side chains that interact with the incoming dNTP and pol with these substitutions retained less than 1% of the wild-type polymerase activity and display a severe decrease in processivity. The signicant stalling of DNA synthesis and extremely low catalytic activities of both mutant enzymes are the two most likely causes of the severe clinical presentation in R943H and Y955C heterozygotes (Graziewicz et al., 2004). The substitution of Tyr955 to cysteine also increases nucleotide misinsertion replication errors 10100 fold in the absence of exonucleolytic proofreading (Ponamarev et al., 2002). For the majority of the disease substitutions that have been studied in vitro, the biochemical defects correlate with the severity and age of onset found in patients (Chan and Copeland, 2009). Further analysis of disease substitutions as well as structural analysis should aid in the continued understanding of disease mutations in the POLG gene.

O.I. Lavrik (B) Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia e-mail: lavrik@niboch.nsc.ru
H.P. Nasheuer (ed.), Genome Stability and Human Diseases, Subcellular Biochemistry 50, DOI 10.1007/978-90-481-3471-7_13, C Springer Science+Business Media B.V. 2010

DBD dsDNA ERCC1 FAP-dCTP

FAP-dUTP
FAP Flu CS GFP GG-NER NER PCNA RPA ssDNA TFIIH TTD UbL UV-DDB UvrABC XP
DNA binding domain double-stranded DNA excision repair cross-complementing rodent repair deciency, complementation group 1 exo-N-[2-N-(N-(4-azido-2,5-diuoro-3-chloropyridine-6yl)-3-aminopropionyl)-aminoethyl]-deoxycytidine-triphosphate 5-{N-[N-(4-azido-2,5-diuoro-3 chloropyridine-6-yl)-3aminopropionyl]-trans-3-aminopropenyl-1}-deoxyuridine -triphosphate uoro-arylazido uoresceinyl Cockayne syndrome green uorescent protein global genome repair nucleotide excision repair poliferating cell nuclear antigen replication protein A single-stranded DNA transcription factor IIH tichothiodistrophy ubiquitin-like UV-damaged DNA-binding protein UV resistant protein ABC xeroderma pigmentosum
Cellular DNA is permanently damaged by endogenous reactive metabolites and exogenous factors. The genetic stability of the organism is maintained by several repair mechanisms (Lindahl and Wood, 1999; Hoeijmakers, 2001; Schrer, 2003; Sancar et al., 2004), among them nucleotide excision repair (NER) plays an important role. NER is the pathway that removes the most types of damages generated in DNA by exogenous agents. Endogenous damage as a rule is producing helix-distorting (bulky) DNA lesions. Defects in NER bring to serious inherited recessive diseases, such as xeroderma pigmentosum (HR), Cockayne syndrome (CS), and tichothiodistrophy (TTD) (Bootsma et al., 1998; Berneburg and Lehmann, 2001). Patients with XP syndrome are highly sensitive to sunlight, and many of them develop skin cancer. NER is an intricate process and depends on the coordinate functions of more than 30 polypeptides (Wood et al., 2001; Gillet and Schrer, 2006). There are two repair pathways, which differ only in the step of recognition of the DNA lesion: global genome repair (GG-NER), which removes lesions from total genomic DNA, and transcription-associated repair, which is restricted to removing
Nucleotide Excision Repair in Higher Eukaryotes

Apoptosis: General Considerations
Apoptosis is the best known form of programmed cell death, dened for the rst time by Kerr, Currie and Wyllie (Kerr et al., 1972) as an event reminiscent of the falling of leaves from trees. Apoptosis is activated in a number of developmental conditions and during cell life can be triggered by external stimuli, among which different types of damage either to DNA or to different cellular components (Scovassi, 2006) (see below). Apoptotic cells can be easily identied by typical morphological changes, such as shrinkage, chromatin aggregation and loss of plasma membrane integrity, which ultimately originate the apoptotic bodies, consisting of chromatin fractions sorrounded by a thin layer of blebbed plasma membrane. Apoptosis triggers cell death without rising any inammatory response, given that apoptotic bodies are eliminated by phagocytosis through the recognition of peculiar eat-me signals of various nature (Gregory and Brown, 2005). Concomitantly to the above described morphological hallmarks, typical biochemical reactions take place, including controlled and organized DNA and protein digestion, operated respectively by specic endonucleases and proteases (Scovassi and Torriglia, 2003; Counis and Torriglia, 2006). Among the latter enzymes, the best known are caspases (cystein aspartate proteases), so called because they contain a cystein residue in their catalytic site and cleave their substrates after an aspartic acid (Kumar, 2007). Caspases exist as inactive zymogens that are proteolytically converted into active enzymes in response to apoptotic stimuli (Shi, 2004). Apoptotic caspases include initiator caspases (caspase-2,-8,-9 and -10) which start
the proteolytic cleavage and effector caspases (-3,-6 and -7) that dismantle the cell by digesting a number of proteins (Scovassi, 2005; Giansanti and Scovassi, 2008a). Some targets of caspases are involved in the maintenance of cell structures, others control DNA metabolism (Luthi and Martin, 2007; Li and Yuan, 2008); the best known is the DNA repair protein poly(ADP-ribose)polymerase-1 (PARP-1), whose proteolysis is considered as a hallmark of apoptosis (Soldani et al., 2001; Soldani and Scovassi, 2002; Scovassi and Diederich, 2004) (see Introduction). Apoptosis occurs mainly through two different pathways: the extrinsic and the intrinsic one, the former being mediated by soluble molecules that interact with outer membrane receptors, and the latter triggered by mitochondria-converging stimuli. Indeed, these pathways are not completely distinct and partially overlap, both converging to the activation of effector caspases (Fadeel and Orrenius, 2005; Green, 2005; Letai, 2008). As illustrated in Fig. 1, the extrinsic pathway is triggered by external stimuli leading to the activation of initiator caspase-8, whereas intrinsic signals converge to mitochondria and promote the activation of caspase-9.