Moticam Pro
CCD Powered Digital Microscopy

FULL PACKAGE

The Moticam Pro range consists of 12 feature rich options providing a large platform for highquality digital microscopy. A Moticam Pro is a sensitive piece of equipment designed to deliver high-quality CCD based images and yet still be affordable and exible enough for a large variety of applications. Choose from Colored / Monochrome and Standard / Peltier Cooled options. The Moticam Pro marks an extension of Motics Camera solutions from the affordable high-resolution CMOS market to the scientic grade CCD range while still offering many choices. Whether your application calls for a Full Color 5.0MP camera for documentation or a Cooled Monochrome camera with 6.45 x 6.45 micron pixels for low light microscopy, there is a Moticam Pro available for you. Designed exclusively in our new labs in Vancouver BC, and assembled under clean-room conditions in our Factory Headquarters, these cameras are ready to be shipped at a moments notice. As with all Motic Digital Solutions, the Moticam Pro is shipped with a full complement of Application Software and Tools to get you started right away.
CCD POWERED DIGITAL MICROSCOPY
www.moticampro.com www.motic.com
BRING SOME COLOR TO YOUR LAB The new Moticam Pro extended range of imaging cameras brings you plenty of performance with minimal impact on your budget.
EFFICIENT COOLING INFORMATION

MAXIMUM

This new range of CCD cameras features optional Peltier Cooling devices, which can bring down the ambient temperature up to 10 degrees Celsius. Our software will constantly show you both Ambient and Sensor Temperature.
DONT LEAVE ANYTHING TO CHANCE
EXPAND YOUR POSSIBILITIES TODAY

More Than Microscopy 2

The Moticam Pro is a vital component in an efcient Lab

Simplied Microscopy

Motics new range of CCD cameras are designed to be used as a valuable tool in the Digital Microscopy market and as a further step of our commitment to quality and a solution to the customers needs. Within the simple yet efficient solid metal housing sits technology designed in our own Canadian development labs. Using Sonys ICX range of sensors exclusively, the cameras are assembled and tested to the highest standards under Clean Room conditions in our own factories. Having invested time and resources into the development of the Moticam Pro range, Motic is now able to offer cameras ranging from High Resolution Color to High Sensitivity Cooled Monochrome Systems.

QUICK CONNECTIONS

Mount the Moticam Pro into an existing installation using the integrated tripod thread A standard mini USB2.0 connection supplies data as well as power for the non-cooled cameras. (USB cable supplied) Use the SDK to integrate the onboard TTL trigger functions. (Trigger cable not supplied)

LIVE READOUT

Create a Temperature Log by letting the Camera record the temperature at a customer chosen interval The cooled MoticamPro feature built-in sensor arrays that allow the user to see updated Sensor and Ambient Temperature as well as the Relative Humidity inside the casing eliminating the possibility of condensation brought on by rapid cooling. Repeatability can also be facilitated by setting a Target Temperature.

IMAGE MANAGEMENT

Manage live, still, time-lapse and video captures through the provided Motic application software. Motic Images Plus is provided right out of the box. For more feature-rich software, Motic Images Advanced is available as a free download for PC users. The MoticamPro uses exclusively Sony ICX sensors providing sustained quality readouts. Choose your camera from a variety of sensors depending on your application and your budget.

More Than Microscopy 4

Inside the MOTICAM PRO
The Moticam Pro is exclusively designed and manufactured in-house. This range of cameras offers many possibilities and options but never looses Motics out-of-the-box approach.
Moticam Pro 252A 252B 282A 282B 205A 205B 205C 205D 285A 285B 285C 285D
Sony Sensor ICX252AQ ICX252AQ ICX282AQ ICX282AQ ICX205AK ICX205AK ICX205AL ICX205AL ICX285AQ ICX285AQ ICX285AL ICX285AL

Sensor Size 1/1.8"

Pixel Size (Micron) 3.45 X 3.45
Resolution (Pixel) 2080 X 1542

Features Color Color With Peltier Cooling Color Color With Peltier Cooling Color

2/3"

3.40 X 3.40

2580 X 1944

1/2"

4.65 X 4.65

1360 X 1024
Color With Peltier Cooling Monochrome Monochrome With Peltier Cooling Color

6.45 X 6.45

Color With Peltier Cooling Monochrome Monochrome With Peltier Cooling
The Moticam Pro is a vital addition to uorescence microscopy

Moticam Pro 252A&B

1.0 1.0

Moticam Pro 282A&B

Moticam Pro 205A&B

Relative response

650 700

Wave length

Moticam Pro 205C&D
1 1.0 0.9 0.8 0.8 0.7 0.6 0.6

Moticam Pro 285A&B

1.0 0.9 0.8 0.7 0.6

Moticam Pro 285C&D

0.5 0.4 0.3 0.2 0.700

0.5 0.4 0.3 0.2 0.1000

900 1000

Hardware

Sony ICX CCD imaging sensor Motic control, processing & imaging Boards Built-in 4 frame buffer* Built-in FPGA* TTL trigger port* Schott BG-40 filter CS/C mount (C-ring provided) Usb2.0 Data mini port (USB cable provided) Universal power supply (for cooled cameras only) Peltier cooling device (for cooled cameras only) Cooling up to 10 degrees below ambient with temperature readout * Unlockable with SDK integration

Software

MOTIC IMAGES PLUS application software MOTIC IMAGES ADVANCED application software for windows computer only* Twain Drivers Motic SDK Motic direct show drivers Motic MI devices live imaging module with live calibration, grid, scale cross and scale bars * Available through free download
Recommened computer specifications
Intel Centrino 1,6 GHz or better 1GB RAM or better (2GB recommed if using shared memory graphics card) USB2.0 Windows XP (SP3) or better Macintosh OSX

More Than Microscopy 6

www.motic.com

More Than Microscopy

Motic Incorporation Ltd. (HONG KONG) Rm 2907-8, Windsor House, 311 Gloucester Road, Causeway Bay, Hong Kong Tel: 852-Fax: 852-Motic Instruments Inc. (CANADA) 130-4611 Viking Way Richmond, B.C. V6V 2K9 Canada Tel: 1-877-Fax: 1-604-Motic Deutschland GmbH (GERMANY) Christian-Kremp-Strasse 11, D-35578 Wetzlar, Germany Tel: 49-6441-Fax: 49-6441-Motic Spain, S.L. (SPAIN) Polgon Industrial Les Corts, Cam del Mig, Cabrera de Mar, Barcelona Spain Tel: 34-93-Fax: 34-93-756 6287
Motic Incorporation Limited Copyright 2002-2009. All Rights Reserved.

Design Change : The manufacturer reserves the right to make changes in instrument design in accordance with scientic and mechanical progress, without notice and without obligation.

Code No.: 1300901303101

Updated: 2009 09

Contents

Driver Installation... 1 User Interface.... 4 Basic Operation of Motic Live Imaging Module.. 5 Language Setting...5 Device Setting & Image Preview..6 Image Adjustment...8 Advanced Setting..13 Video Capture...16
Note: The intention of this guide is to provide quick instructions to key tasks of the Motic Live Imaging Module. If more details are needed or what you are looking for is not contained in this guide, please consult the Help that comes with your software or visit our website http://www.motic.com.

Motic Live Imaging

Motic Live Imaging Module is a professional image adjustment and capture module which is used to improve, edit or change image quality before capturing the final picture. It provides powerful video adjustment, live image transmission and image capturing functions, enabling users to view and adjust the real-time image, capture a still image to the assigned directory or directly transfer a live image into the main software for further processing or analysis.

Driver Installation

The driver files of all camera devices supported by the Motic Live Imaging Module can be found on the installation CD of your main Motic imaging program (for example Motic Images Plus 2.0ML or Motic Images Advanced 3.2) The following example is of a typical driver installation is of a Moticam 2300 (MC3001). Other supported Operating Systems have a similar approach. Ensure the Moticam 2300 (MC3001) is plugged into the computer. The Find New Hardware Wizard will appear. With the software CD in the CD driver, select Install the software automatically. If this fails to install the driver, select Install from a list or specific location (Advanced), and click Next.
Select Include this location in the search. Click Browse to select the driver.
Locate the Motic MC Camera V3 folder under Driver in the CD files (or select the corresponding driver according to the instructions on the CD case, or see the chart below) and click OK. Device
Camera 3 PAL USB 1.0 USB 2.0 Camera 3 NTSC USB 1.0 MC1001/MC2001/ USB 2.0 MC 3001 V3 MC1001/MC2001/ MC3001/1002/2002 (for Vista) MC352 MC352+ MD1500/MD3000/ IEEE1394 MD5000 MD5000 USB 1.0 USB 1.0 USB 2.0 Motic USB2 Camera MC352 MC352+ MD3000/ Camera AD640 Motic MC M210 M230

Interface

USB 2.0

Driver

Note: If you use M230, please ensure that: 1. The driver is installed under Windows XP or Windows 2000 + SP4 operating system; 2. USB 2.0 driver and DirectX9.0b (or higher version) have been correctly installed.
Please confirm the destination path, and click "Next".
Click Continue Anyway to continue.
Files will be copied to your computer.
Click "Finish".

User Interface

The Motic Live Imaging Module contains Title bar, Control Panel, Image Preview Window and Status Bar. Title Bar: Shows the module name. Control Panel: Four control panels are available. Click one of the following four buttons to display the corresponding control panel: Basic Adjustment panel;
Color Adjustment panel; Advanced Setting panel; Video Capture panel. Click the button display it again. Image Preview Window: Displays the real-time live image coming from your Moticam or Digital Microscope. Click the button again. Status Bar: Shows the current frame rate you can increase the frame rate by increasing illumination and decreasing the exposure rate, or by lowering the live resolution on the title bar to hide this window, re-click to display it on the title bar to hide the control panel, re-click to
Basic Operation of Motic Live Imaging Module

Language Setting

From the Language dropdown list, select a language of your choice.
Device Setting & Image Preview
Connect an imaging device to your computer and open this image Module, the connected device will be auto-opened and the live image will display. Note: When several Motic devices have been connected and the auto-opened device is not what you want, please click "Close", select the desired one from the dropdown list and click "Open". Note: When connecting with our low resolution devices, the Property dropdown list will be activated allowing you to adjust more chip parameters. Note: When connecting our Moticam 3000/5000 series, the Fast Display checkbox will be activated allowing you to start the Real Time Viewing (RTV) mode.
The Resolution drop-down menu allows you to switch between several resolution settings. These settings change the amount of data that are being transmitted to the computer by the imaging device. The lower the resolution the higher the frame rate. By clicking on the Fit to Window box, the live image will be automatically resized to fit into the display window therefore allowing you to view the cameras full Field of View even while keeping the resolution high. The width to height ratio will not change To view only a selected part of the image in full resolution, click and drag a Region Of Interest area on the live image with your mouse then click on the ROI Preview button. Note: You can change the shape and outline color of your ROI selection by clicking the ROI Property settings. Click the Full button to view the live image in full screen mode. Press Esc to exit the full screen mode.

Image Adjustment

1. Basic Adjustment It is recommended that you do basic image adjustment according to the following steps:

1) Click the button

to display the Basic Adjustment panel. Setup your microscope properly, focus on your slide, then move the slide away from your cameras field of view and drag the Exposure slider for manual, or click on the Auto box for automatic exposure. Note: Some camera chips have built-in
hardware fine auto-exposure controls and so will also adjust the exposure. If you wish to override this, use the slider to adjust the settings manually.
The Background Balance function will help reduce the effects of uneven illumination. To use it properly, please setup your microscope, focus on your slide and adjust the microscopes illumination and the Exposure settings above accordingly. Next move the slide away from the cameras Field of View and click on the Background Balance button. The screen will then be even. You can adjust exposure settings if you wish and also perform the White Balance function below. Next, move the slide back into the cameras field of view. Note: When you change the microscopes objective or adjust the microscopes illumination intensity, please perform the above steps again.
The White Balance button will help get the best Color similar to what you see through the eyepieces. Setup your microscope properly, focus on your slide, then remove the slide from the Field of View and click on the White Balance button. Next, you can move the slide back into the cameras view. Note: Some imaging chips will have an
automatic hardware white balance. You will see the white balance being adjusted automatically as close to the real color as possible. In order to adjust the color even closer, please use the tools in the Color Adjustment Panel. Note: Each Apply checkbox will be auto checked while clicking the Background Balance and White Balance buttons. Recheck it to cancel the corresponding function.
You can also adjust the gain, offset and other values by dragging the corresponding slider bar to improve the image quality; if the image is displayed upside down, check Mirror or Flip to correct it.

2. Color Adjustment

Click the button
to display the Color Adjustment panel, in which you are
able to do some color adjustments to the image if necessary. Color Correction Click on Enable to activate this function. Then drag the slider bar to a suitable setting for the intensity of the correction. This function was designed to distinguish between Blue, Red and Purple and will enhance in that spectrum. This is useful especially for H&E stained specimens.
RGB Adjustment For full manual color control, you can adjust both Gain and Brightness of a specific color separately.

To go back to the default camera settings, click on Reset.
You can display a color Histogram by clicking on the button. This will show the pixel gray scale distribution of the entire image or a user defined ROI area.

default,

Histogram

window

displays the histogram of the entire image. Right click within the window and select the ROI Histogram command to display the histogram of the user defined ROI area, click the command again to cancel it. Note: Please define an ROI area before selecting Histogram. the command ROI

Advanced Setting

Click the button to set advanced settings. From the Filter Setting group box, check the corresponding checkbox to enable the function of Filter, Edge Detection or Sharpness. You can choose a specific filter from the dropdown list, and specify the values of edge detection and sharpness by dragging the slider bars. Note: Filter and Edge Detection functions only work on an ROI area. Sharpness function only works on the entire image. Check Remove Noise and select 1, 2, 3 or 4 from the dropdown list to remove any noise from the current image. The larger the value, the greater the effect, and more system resources will be consumed causing the live image to appear slower. Note This function only works on the entire image.
You must do the live calibration before using Grid, Scale Cross or Scale Bar tools. Click calibration button following to the

perform window. Note:

instructions on the popup Calibration
You will need the calibration slide. Click the Calibration button in the Calibration window to display the Save dialog. First input a sign name or select a sign name from the left list, then click the Save button to save the result to the Calibration table for a later use.

button, and select

an item from the Calibration Table window, then click ok to apply the calibration results to the current image. Note: In the initial state, the data saved in this table is the initial data of each objective magnification before calibration and is marked column. false Please in the Calibration calibration perform

before you apply it.

Having done the calibration, check the corresponding checkbox below the Calibration button to analyze the image with Grid, Scale Cross or Scale Bar. Users are able to set the properties of each function. Note: The unit of calibration circle selected from the Calibration window will be the units of Grid, Scale Cross and Scale Bar.

You can set the shape and outline color of the ROI defined on the image. Users are able to save and load the parameter settings that have been set within the Basic Adjustment panel, Color Adjustment panel, and Advanced Setting panel. Note: A name should be input in the blank before saving the corresponding
parameter settings. Check Cooler Enabled to enable the Peltier camera cooler. Note: Only available with Cooled Cameras. 15

Video Capture

Click the button to display the Video Capture panel, with which you are
able to capture images and record videos.
1. Image Capture Select a size at which to capture an image from the Format dropdown list. Click the "Capture" button to capture the real-time image shown in the preview window to the main software; if you open this module directly without running the main software, a dialog will appear for you to define the image saving path when you click the "Capture" button. To capture an ROI image, hold down the left mouse button and drag the mouse to define an ROI on the image then click Capture. To auto capture images, click the "AutoCapture" button and specify the auto capture settings from the popup dialog, then click "OK" to capture a sequence of still images according to the preset settings.
2. Video Recording To record video, click the "Record" button and specify the video settings from the popup dialog, then click "OK" to start recording video according to the preset settings. Click the "Stop" button to stop recording at any time. Note: It is recommended that you download the Xvid Codec in order to capture smaller size video files.