Wireless Rain Gauge with Indoor / Outdoor Thermometer Model: RGR126 User Manual
CONTENTS Introduction.. 2 Product Overview.. 2 Front View.. 2 Back View.. 3 LCD Display... 4 Rain Gauge (PCR122).. 4 Remote Thermo Sensor (THN122N). 5 Getting Started.. 6 Main Unit... 6 Rain Gauge (PCR122).. 6 Thermo Sensor (THN122N).. 7 Data Transmission.. 9 Low Battery Warning.. 9 Clock / Calendar Setting.. 9 Set Clock & Calendar.. 9 Rainfall... 10 Rainfall History... 10 Total Rainfall... 10 Rainfall Alarm.. 10 1
Temperature.. 11 Outdoor... 11 Indoor.. 11 Reset... 11 Precautions.. 11 Technical Specifications.. 12 Notices... 12 Statement of Responsibility.. 12 About Oregon Scientific.. 13 FCC Statement.. 13 Declaration of Conformity. 14

RGR126(R13)-4(final)

4/6/05, 5:46 PM

INTRODUCTION

Congratulations on purchasing the Oregon Scientific Wireless Rain Gauge with Indoor / Outdoor Thermometer (RGR126). The rain gauge is equipped with the following functions: Daily and total rainfall display 9-day rainfall history Rainfall alarm Indoor / outdoor temperature display Real-time clock and calendar setting 328-foot (100-meter) transmission range

PRODUCT OVERVIEW

FRONT VIEW
The RGR126 operates at 433MHz. No wire installation is required. 6 7
8 1. FOUR-LINE LCD DISPLAY: For displaying the total rainfall, current rainfall or rainfall history, indoor / outdoor temperature and clock / calendar SEARCH: Press and hold to enforce a search for the rain gauge and remote thermo sensor in - mm: Selects between inch and millimeter rainfall measurement

2. 3. 2

RGR126(R13)-4(final) 2
((.)): Displays the rain alarm setting; press and hold to enter the rain alarm setting mode SINCE: Toggles between displaying the start day or start year for total rainfall; press and hold to clear the previous rainfall start time and reset the rainfall counter to start again HISTORY: Toggles between rainfall history and daily rainfall : Enables or disables the rainfall alarm REMOVABLE TABLE STAND: For securing the main unit on a flat surface

1. 2. 3. 4. 5.

ANTENNA: Receives radio signal from rain gauge and remote thermo sensor : Increases setting values; press and hold for rapid increase : Decreases setting values; press and hold for rapid decrease WALL MOUNT HOLE TIME / SET: Toggles among the time, day, year and rainfall display; press and hold to enter the time setting mode RESET: Returns all settings to their default values C / F BUTTON: Selects between C and F BATTERY COMPARTMENT: Accommodates 2 x UM-3 or AA-sized batteries

6. 7. 8.

BACK VIEW
RGR126(R13)-4(final) 3 4/6/05, 5:46 PM

LCD DISPLAY

RAIN GAUGE (PCR122) 1. ANTENNA: Transmits radio signal to main unit BATTERY COMPARTMENT: Accommodates 2 x UM-3 or AAsized batteries

1. 2. 3. 4.

Today's Rainfall, Rainfall History, Rainfall Alarm, Start Date of Total Rainfall Record, Time / Date Rain Gauge RF Reception Status, Total Rainfall Thermo-sensor RF Reception Status, Outdoor Temp Indoor Temp 2
RGR126(R13)-4(final) 4 4/6/05, 5:46 PM
REMOTE THERMO SENSOR (THN122N) 1. LED Indicator 1 1. 2. 3. 1 4. 4 WALL MOUNT HOLE RESET hole CHANNEL number (1-3) BATTERY COMPARTMENT
RGR126(R13)-4(final) 5 4/6/05, 5:46 PM

GETTING STARTED

Position the main unit, rain gauge, and thermo sensor within effective range: Rain gauge - 328 feet / 100 meters Thermo sensor - 98 feet / 30 meters IMPORTANT It is recommended that you follow the sequence below when setting up the units: 1. 2. Set up the main unit. Set up the rain gauge, then press and hold SEARCH on the main unit and wait until you receive a signal. Set up the thermo sensor, then press and hold SEARCH on the main unit and wait until you receive a signal. In the event that you do not obtain a signal from a remote unit please repeat the process above.
Press RESET, or press and hold SEARCH on the main unit to initiate operation. The reception icon on the main unit will blink while it is searching for the sensor or rainfall gauge.
NOTE Every time the battery in the main unit is changed, repeat installation before use. RAIN GAUGE (PCR122) 1. Insert the batteries, matching the polarity (+ and -) as shown in the battery compartment.

"AA" UM3 1.5V

MAIN UNIT 1. Insert the batteries, matching the polarity (+ and -) as shown in the battery compartment.
Mount the rain gauge on a level surface.

RGR126(R13)-4(final) 6 4/6/05, 5:46 PM
Put drops of water on the cross at the base to check the leveling.
Remove the fiber tape from around the bucket assemblies.
THERMO SENSOR (THN122N) 1. Insert the batteries, matching the polarity (+ and -) as shown in the battery compartment.
Use the metal ring to adjust the level of the rain gauge.

2. 3. 4. 7

RGR126(R13)-4(final) 7
Set the channel switch to any channel. The switch is located in the battery compartment. Press RESET on the sensor. Close the remote sensor battery compartment.
Secure the sensor in the desired location using the wall mount or table stand.
Place the sensor in a location with a clear view to the sky, away from metallic or electronic objects. Position the sensor close to the main unit during cold winter months as below-freezing temperatures may affect battery performance and signal transmission.
You may need to experiment with various locations to get the best results. Standard Alkaline batteries contain significant amounts of water. Because of this they will freeze in low temperatures of approximately 10F (-12C). Disposable Lithium batteries have a much lower threshold for temperature with an estimated freezing range of below -40F (-40C). Wireless ranges can be impacted by a variety of factors such as extremely cold temperatures. Extreme cold may temporarily reduce the effective range between the sensor and the base station. If the unit's performance fails due to low temperature, the unit will resume proper functioning as the temperature rises to within the normal temperature range (i.e. no permanent damage will occur to the unit due to low temperatures).
For best results: Insert the batteries before you mount the sensor. Place the sensor out of direct sunlight and moisture. Do not place the sensor more than 98 feet (30 meters) from the main (indoor) unit. Position the sensor so that it faces the main (indoor) unit, minimizing obstructions such as doors, walls, and furniture. 8

RGR126(R13)-4(final) 8

DATA TRANSMISSION Data is sent from the remote thermo sensor approx. every 78 seconds and every 94 seconds from the rain gauge. The reception icon shows the status - see table.
THERMO SENSOR RAIN GAUGE DESCRIPTION Searching Connected Disconnected

LOW BATTERY WARNING

There are 3 low-battery indicators - see table.
UNIT Main Remote Thermo Sensor Rain Gauge LOCATION ON MAIN UNIT DISPLAY Indoor Temperature Area Outdoor Temperature Area Total Rainfall Area
Replace the batteries immediately when the respective indicator lights up.
If without obvious reason the main unit display shows that the thermo sensor or rainfall connector is disconnected, press and hold SEARCH to enforce an immediate search.N If that fails, check: The remote rain gauge and thermo sensor are still in place. The batteries of the main unit, rain gauge, and thermo sensor are still good. Replace them if necessary. The transmission is within range and path is clear of obstacles and interference. Shorten the distance if necessary. Then press and hold SEARCH again.

CLOCK / CALENDAR SETTING

Press TIME / SET to toggle among today's rainfall, time, date, and year. SET CLOCK & CALENDAR 1. 2. 3. 4. 5. 6. Press TIME / SET to display the clock or calendar. Press and hold TIME / SET for two seconds. Use or to set the desired value. Press TIME / SET to confirm. Repeat steps 3 and 4 to complete all settings. The order of the settings is hour, minute, year, month and day.
RGR126(R13)-4(final) 9 4/6/05, 5:46 PM

RAINFALL

Today's rainfall appears on the 1st line of the display and total rainfall is shown on the 2nd line. Press in / mm to toggle between inches and millimeters as the unit of measurement. RAINFALL HISTORY The rainfall history is displayed on the 1st line of the LCD display. The main unit can record up to 9 days of rainfall. Use HISTORY to display the record for a particular day. The day of the record will be displayed with a minus (-) sign. Caution: Other sensors using the 433 MHz transmission frequency may influence the rainfall reading. Please avoid placing those sensors too close to the unit. TOTAL RAINFALL The total rainfall is displayed on the 2nd line of the LCD display. To display the commencing date of the total rainfall record, press SINCE. The date will appear on the 1st line of the display. Press again to show the commencing year. 10

RGR126(R13)-4(final) 10

To clear the existing commencing date and reset it to start again, press and hold SINCE. The total rainfall will now be equal to the today rainfall and the unit will start again to collect the rainfall data. RAINFALL ALARM The rain alarm value can be displayed at any time by pressing (( )). To set the rainfall alarm, 1. 2. 3. Press and hold (( )) for two seconds. Use or to set the desired value. Press (( )) to confirm. The alarm will be activated. The rain alarm indicator will light up.
The alarm will go off for one minute when the rainfall reaches the preset value and the rain alarm indicator will flash. A reminder beep will be emitted every minute. Once the rain alarm is on the indicator will not stop flashing unless is pressed or the rain alarm value is changed. To stop the alarm and reminder beep, press any button or change the rainfall alarm value. To deactivate the alarm, press.

TEMPERATURE

OUTDOOR The outdoor temperature is displayed on the 3rd line of the LCD display. Use the C / F button to select between Centigrade and Fahrenheit display units. INDOOR The indoor temperature is displayed on the 4th line of the LCD display. Use the C / F button to select between Centigrade and Fahrenheit display units.

PRECAUTIONS

This product is engineered to give you years of satisfactory service if you handle it carefully. Here are a few precautions: Do not immerse the unit in water. Do not clean the unit with abrasive or corrosive materials. They may scratch the plastic parts and corrode the electronic circuit. Do not subject the unit to excessive force, shock, dust, temperature or humidity, which may result in malfunction, shorter electronic life span, damaged battery and distorted parts. Do not tamper with the unit's internal components. Doing so will invalidate the warranty on the unit and may cause unnecessary damage. The unit contains no user-serviceable parts. Only use fresh batteries as specified in the user's manual. Do not mix new and old batteries as the old ones may leak. Always read the user's manual thoroughly before operating the unit.
This button should only be used when the unit is operating in an unfavorable way. Use a blunt stylus to hold down the button. All settings will return to their default values.
RGR126(R13)-4(final) 11 4/6/05, 5:46 PM

TECHNICAL SPECIFICATIONS

TYPE Main Unit Dimensions LxWxH Weight (without battery) Main unit Display rainfall range (total) Display rainfall range (history / daily) Rainfall resolution Operating range DESCRIPTION 3.54 x 0.98 x 5.59 inches (90 x 25 x 142 mm) 0.38 lbs (173 g) 0 - 999.99 inch (0 - 25,400 mm) 0 - 99.99 inch (0 - 2,540 mm)
General RF Transmission Frequency 433 MHz RF Transmission Range Rain gauge - 328 feet (100 meters) Remote thermo sensor - 98 feet (30 meters) Temperature sensing cycle Around 78 seconds Rainfall sensing cycle Around 94 seconds NOTE It is recommended that you use alkaline batteries with this product for longer performance or lithium batteries in temperatures below freezing. NOTE Design and specifications are subject to change without notice.
0.04 inch (1 mm) 23F to 122F (-5C to +50C) Temperature resolution 0.2F to (0.1C) Remote Rain Gauge (PCR122) Operating range 33.8 F to 140.0F (1.0 C to 60.0C) Weight 0.57 lbs (260 g) Remote Thermo Sensor (THN122N) Proposed operating range -22 F to 140F (-30 C to 60C) Power Main unit 2 x UM-3 / AA 1.5V batteries Rain gauge 2 x UM-3 / AA 1.5V batteries Remote thermo sensor 2 x UM-4 / AAA 1.5V batteries 12

RGR126(R13)-4(final) 12

NOTICES
STATEMENT OF RESPONSIBILITY Oregon Scientific will not be held liable for any bodily injury, death, property damages, or any other claims of whatever nature resulting from the misuse or negligence of the product, whether intentional or unintentional. Oregon Scientific will not be responsible for any deviations in the usage of the device from those specified in the user instructions or any approved alternations or repairs of the product.

ABOUT OREGON SCIENTIFIC

Visit our website (www.oregonscientific.com) to learn more about Oregon Scientific products such as digital cameras; MP3 players; children's electronic learning products and games; projection clocks; health and fitness gear; weather stations; and digital and conference phones. The website also includes contact information for our Customer Care department in case you need to reach us, as well as frequently asked questions and customer downloads. We hope you will find all the information you need on our website, however if you're in the US and would like to contact the Oregon Scientific Customer Care department directly, please visit: www2.oregonscientific.com/service/support OR Call 1-800-853-8883. For international inquiries, please visit: www2.oregonscientific.com/about/international/default.asp

FCC STATEMENT

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) This device must accept any interference received, including interference that may cause undesired operation. WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user's authority to operate the equipment. NOTE This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

RGR126(R13)-4(final) 13 4/6/05, 5:46 PM
Reorient or relocate the receiving antenna. Increase the separation between the equipment and receiver. Connect the equipment into an outlet on a circuit different from that to which the receiver is connected. Consult the dealer or an experienced radio / TV technician for help.

Address:

Block C, 9/F, Kaiser Estate, Phase 1,41 Man Yue St., Hung Hom, Kowloon, Hong Kong
DECLARATION OF CONFORMITY The following information is not to be used as contact for support or sales. Please call our customer service number (listed on our website at www.oregonscientific.com), or on the warranty card for this product) for all inquiries instead. We Name: Address: Telephone No.: Fax No.: Oregon Scientific, Inc. 19861 SW 95th Ave., Tualatin, Oregon 97062 USA 1-800-853-8883 1-503-684-3332
is in conformity with Part 15 of the FCC Rules. Operation is subject to the following two conditions: 1) This device may not cause harmful interference. 2) This device must accept any interference received, including interference that may cause undesired operation.
declare that the product Product No.: RGR126 Product Name: Wireless Rain Monitor Manufacturer: IDT Technology Limited
RGR126(R13)-4(final) 14 4/6/05, 5:46 PM

Cell Culture people you can contact today.

Tina Mclure (Australia)

Product Specialist Cell Culture tina.mclure@thermofisher.com ph: +Tina has been involved in the sales and product management of tissue culture products over the past 5 years and has just returned from visiting the Nunc and Nalgene manufacturing sites. Tina is very excited with the introduction of a number of new and innovative products for tissue culture work for 2010 and 2011.
The Thermo Fisher Scientific Story
Australia & New Zealand
Thermo Fisher Scientific is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. In April 2009 Thermo Fisher Scientific made a major investment in the Australasian region acquiring the distribution company Biolab which in itself has a 150 year history. By doing so, Thermo Fisher has taken the first steps to bringing together worldwide leading products and brands into the one company. With our continued commitment and investment into R&D, analysis, discovery and diagnostics, Thermo Fisher Scientific today is leading the way with an unparalleled product portfolio and service offering, combined with a customer-focused technical sales capability. Globally, Thermo Fisher Scientific has 33,000 employees from 38 countries all sharing in the same mission and core values expressed as the four Is: Integrity, Intensity, Innovation, and Involvement. So it is a great pleasure to have the opportunity to relaunch the previously known publication Helix, with the aim of sharing one of those key values by renaming the publication Bio-Innovation. Bio-Innovation will be published quarterly and will provide researchers with the latest updates on new applications and methodology (including new products and technologies) with the first issue focusing on Cell Culture. This issue will also be available at the forthcoming Australian Society of Immunology meeting, Perth, 5-9 December 2010. Our next issue will be focussed on molecular biology.
Peter Chisholm (Australia)
Product Manager Cell Culture and BioProcess peter.chisholm@thermofisher.com ph: +Peter has been part of the life science industry for more than 30 years, and still loves what he is doing. Peter is always willing to share his industry-wide experience and expertise particularly in the Bioprocessing domain. Peters key responsibility is HyClone the quality name in cell culture media, sera and bioprocessing containers.

Jerry Wong (New Zealand)

Product Specialist Cell Culture jerry.wong@thermofisher.com ph: +Jerry completed his Bachelor of Technology honours degree (Biomedical Science) at the University of Auckland, and worked at Vialactia Biosciences Ltd before embarking on completing his PhD at the University of Auckland. Jerrys research was in molecular neuroscience to investigate novel therapeutic strategies for Parkinsons disease. Jerry joined our team early this year and will bring his extensive knowledge to our cell culture team. 2
Christopher Hum Business Development Manager Biosciences

Application Note

Contents

Articles

Outstanding Observation: A Faster Way To Publish Your Groundbreaking Findings Stem Cell PromiseResearch Brings Autograft Revolution Closer Choosing the Right Centrifuge for Your Application 22

Applications

Technical Bulletin: Cell Adhesion & Growth UpCell Surface versus Trypsinisation and Scraping in Cell Detachment
Take Your Cell-Based Assays To The Edge / Vita Means Life A Novel Feeder-Free Embryonic Stem Cell Culture System Endothelial Progenitors Encapsulated In Bioartificial Niches Are Insulated From Systemic Cytotoxicity & Are Angiogenesis Competent Real World Advantages In Breast Cancer Analysis Water Quality Standards For Research And Analysis Applications Testing The Efficacy Of The Antimicrobial Treatment A Study Preventing Cell Culture Contamination with Copper CO2 Incubators
Advancing Cell Culture News, Events & Exhibitions

The Next Generation In Automated Liquid Handling When is a L not a L? Chart MVE for Ultra Cold Storage Culture Serum Q & A

article

Outstanding Observation: A faster way to publish your groundbreaking findings
It is often difficult to publish novel findings if they do not also include a detailed description of the molecular mechanisms involved. Researchers may find that the dissemination of their groundbreaking discoveries is delayed by months or even years as they try to provide the in-depth mechanistic data required to satisfy the submission criteria for their desired journal.
Professor Chris R Parish, Editor-inChief, Immunology and Cell Biology
In an effort to break through this barrier and to allow immunologists to publish their paradigm-shifting results without getting held up with the mechanistic basis of the observations, Immunology and Cell Biology launched the Outstanding Observation series in 2008. Owing to the cutting-edge nature of this type of article, papers are expedited through the refereeing process and are therefore published more rapidly than standard articles.
Polyclonal Treg cells enhance the activity of a mucosal adjuvant Silvia Vendetti, Todd S Davidson, Filippo Veglia, et al.Immunology and Cell Biology (2010) 88, 698-706 CD69 limits early inflammatory diseases associated with immune response to Listeria monocytogenes infection Javier Vega-Ramos, Elisenda Alari-Pahissa, Juana del Valle, et al. Immunology and Cell Biology (2010) 88, 707-715
Scan these tags with your mobile phone for instant access to these articles. Download the free app for your phone at http://gettag.mobi
Access these articles free of charge via the
I would like to invite you to sample 2 recent examples of this article type:
Immunology & Cell Biology website. www.nature.com/icb
Congratulations to Dr Cindy Ma, Immunology and Inflammation, Garvan Institute of Medical Research, NSW, Australia, winner of the 2009 Outstanding Observation ICB Publication of the Year award. The award is a AU$1000 scholarship provided by the Nature Publishing Group. Also Congratulations to Dr Mark Dowling, Immunology, Walter and Eliza Hall Institute, VIC, Australia as runner-up and will receive AU$500 travel scholarship provided by Thermo Fisher Scientific.
Technical Bulletin: Cell Adhesion & Growth. A comparison of Coated, Modified Glass and Plastic Surfaces

Results: Cells of the fibroblast-like cell line BHK-21 (baby hamster kidney), which are very adherent, did not distinguish between these growth surfaces. The less adherent fibroblast-like cell line L929 (mouse lung) and two epitheliallike cell lines, HEp-2 (human epidermal) and WISH (human amnion), grew to slightly higher densities and produced more uniform monolayers when grown on electrically modified plastic compared to unmodified plastic or glass. These cell types also grew very well on both types of chemically modified glass surfaces.Primary brain neurons did not adhere to unmodified plastic or glass surfaces. However, neurons adhered and differentiated on polylysine coated glass or plastic surfaces and, albeit differently, on both of the chemically modified glass surfaces. Electrical modification of the plastic or glass, which significantly increased the surface energy or wettability, did not produce a surface suitable for these neurons. Adhesion, growth and differentiation of cells on a surface is cell type specific and involves more than one mechanism. Many cell lines prefer surfaces with a high surface energy such as produced by electrical modification. Other cell types, such as primary neurons, require a specific interaction with functional groups provided by polylysine coating or chemical modification of the growth surface. Conclusions The effect of growth surfaces on adhesion, growth and differentiation of cells is cell-type specific and involves more than one mechanism. Many cells prefer surfaces with high surface energies (i.e. hydrophilic surfaces). Growth surfaces such as CC2 may improve cell adhesion and induce cellular differentiation of fibroblastic-like cells. High surface energy is not sufficient for primary neuron growth. Biological coatings or chemical modifications that place amines on the growth surface facilitate primary neuron adhesion and differentiation. Other variables, such as those that differentiate European and American borosilicate coverglass, may influence neuron survival on glass surfaces.
For more information or to obtain a full copy of this technical bulletin please contact: Tina McLure(AU) tina.mclure@thermofisher.com Ph: +Dr Jerry Wong (NZ) jerry.wong@thermofisher.com Ph: +980 6768

Recovery ratio (%)

UpCell Surface versus Trypsinisation and Scraping in Cell Detachment
This compares the recovery of mouse peritoneal macrophages harvested from the UpCell Surface using temperature reduction with those harvested from traditional cultureware (tissue culture-treated polystyrene) using trypsinisation or scraping.
Methods Mouse peritoneal macrophages in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) were seeded in one dish with UpCell Surface and two traditional cultureware dishes at 2.4 x 105 cells/cm2. The cells were incubated at 37C in a humidified atmosphere of 5% CO2 in air. After 2 hours of incubation, non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Cells were then cultured for 2 days in RPMI 1640 medium supplemented with 10% FBS and harvested using one of the following procedures: Harvest of cells from the UpCell Surface using temperature reduction Non-adherent cells were removed by washing with Ca2+ and Mg2+ free PBS 4.0 mL RPMI 1640 medium supplemented with 10% FBS was added and the dish was incubated at 20C for 30 minutes Detached cells were harvested Harvest of cells from traditional cultureware using trypsinisation Non-adherent cells were removed by washing with Ca2+ and Mg2+ free PBS 1.0 mL of 0.25% trypsin/EDTA was added, and the dish was incubated at 37C for 5 minutes 3.0 mL RPMI 1640 medium supplemented with 10% FBS was added Detached cells were harvested Harvest of cells from traditional cultureware using EDTA and scraping Non-adherent cells were removed by washing with Ca2+ and Mg2+ free PBS 4.0 mL of 2.5 mM EDTA/PBS was added and the dish was incubated on ice for 20 minutes The cells were detached by scraping and harvested

Advantages of Thermo Scientific Nunc Edge Plate
Low-evaporation moat 96 well format - follows ANSI standard footprint and is amenable to automation Simply fill moat with sterile H2O and eliminate edge effects Cell culture treated or non-treated Low fluorescence Custom barcoding available the safest way to track your samples Designed for both automated or manual use Superior imaging
For more information please contact: Tina McLure (AU) tina.mclure@thermofisher.com or Ph: +Dr Jerry Wong (NZ) jerry.wong@thermofisher.com or Ph: +980 6768
Vita means Life : NEW Thermo Scientific Nunc Nunclon Vita Surface
Animal component-free surface for growth of stem cells & other fastidious cells.
Nunclon Vita Surface supports attachment, colony formation and growth of human ESC, human IPS cells and other fastidious cell lines in the absence of feeder cells and matrix coatings. In media supplemented with ROCK inhibitor, human ESC can be cultured on the Nunclon Vita Surface for at least 10 passages without loss of pluripotency. Human ESC has successfully been expanded for more than 10 passages without changes to the karyotype.
The unique Nunclon Vita surface helps remove variability, requires less work, and allows for the treatment surface to be adaptable to scalable cell expansion. Human Embryonic Stem Cells (human ESC) No matrix coating or feeder cells necessary Expand for more than 10 passages in conditioned media with a ROCK inhibitor while maintaining pluripotency Passage with enzymes or by mechanical selection Alternatively, passage by withdrawing ROCK inhibitor followed by a short incubation and gentle pipetting

HyStem

A Novel Feeder-Free Embryonic Stem Cell Culture System that Supports Mouse Embryonic Stem Cell Growth and Proliferation
Kalle Johnson 1, Robin Wesselschmidt 2, Mark Wight1, Thomas I. Zarembinski 3
Figure 1: Phase contrast photographs of mESC colonies over the course of the study. Photos of colonies grown on HyStem-C (top Row) and photos of colonies co-cultured with MEF feeders (bottom row). Morphologies bear slight differences between HyStem-C and MEF co-cultures. However, cultures grown on both substrates were able to maintain colonies that generally exhibited relatively regular, phasebright, well defined borders, characteristic of undifferentiated mESC colonies.

Passage 1

Passage 2

Passage 3

Passage 4

Passage 5

Standard embryonic stem cell culture systems require co-culture with mitotically inactive mouse embryonic fibroblast feeder cells (iMEFs) to maintain their undifferentiated proliferative state. While iMEFs provide a suitable attachment surface and crucial soluble factors promoting embryonic stem cell (ESC) growth and proliferation, iMEFs are timeconsuming to prepare. More importantly, iMEFs vary from lot-to-lot and contaminate ESCs with carry over iMEFs from previous culture passages. These latter shortcomings confound basic research attempting to dissect the culture components or underlying gene and protein expression patterns important for ESC proliferation and differentiation. Development of a feeder-free system for embryonic stem cell culture using a synthetic matrix would provide a ready-to-use substrate which is consistent from lot to lot without iMEF carry over. iMEFs make abundant amounts of hyaluronic acid (HA) which is important for not only embryogenesis but also for human embryonic stem cell culture (1). Using mouse embryonic stem cells (mESCs) as a model system, we reasoned that the use of a commercially available HA-rich matrix would provide a suitable starting point for preparing a novel feeder-free substrate for undifferentiated growth. Here we report the use of a crosslinkable HA-based substrate (HyStem-CTM) for feeder-free propagation of mESCs in the presence of FBS. mESCs plated on HyStem-C maintain excellent morphology and offer comparable plating efficiency to those grown on iMEFs.

Cell Culture

Testing the efficacy of the antimicrobial treatment a study
ELGA LabWaterWater quality standards for Research and analysis applications
Type 1+ Goes beyond the purity requirements of Type 1 water Type I Often referred to as ultra pure, this grade is required for some of the most water-critical applications such as HPLC (High Performance Liquid Chromatography) mobile phase preparation, as well as, blanks and sample dilution for other key analytical techniques; such as GC (Gas Chromatography), AAS (Atomic Absorption Spectrophotometry) and ICP-MS (Inductively Coupled Plasma Mass Spectrometry). Type I is also required for molecular biology applications as well as mammalian cell culture and IVF (In vitro Fertilisation). Type II Is the grade for general laboratory applications. This may include media preparation, pH solutions and buffers and for certain clinical analysers. It is also common for Type II systems to be used as a feed to a Type I system*.
LCP Research Group, Thermo Fisher Scientific, Vantaa, Finland

Advancing

Microbes, such as bacteria, fungi and algae, are found everywhere around us, and they are also present in the human skin. Normally they are not harmful, but in some cases they may cause deterioration of the material they grow on or cause cross contamination. Even when strict cleanliness is observed, microbes from the hands may contaminate any surface. Antimicrobial treatment protects from microbial growth and adds additional protection against cross- contamination. i.e. bacteria from a pipette that could contaminate the sample. How does it work? The active ingredient of the antimicrobial material tested is silver in the form of silver ions. In a humid environment the ions are slowly released from the inorganic matrix via an ion-exchange mechanism. The release is slow, but fast enough to maintain an effective concentration on the surface of the material. Silver ions are taken up by microbial cells and interrupt critical functions, such as DNA replication, resulting in the death of the microbes. The antimicrobial effect of the material used is longterm and silver inhibits the growth of a broad spectrum of microorganisms. Testing the efficacy of the antimicrobial treatment The antimicrobial effect of the material was evaluated according to ASTM standard E21 80. The standard describes a test method to evaluate (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat hydrophobic or polymeric surfaces. The test organisms used were Escherichia coli, Staphylococcus aureus, Candida albicans and conidiospores of Aspergillus niger. Contamination of the antimicrobial polymer pieces (from a Thermo Scientific Finnpipette F1 - The handle and the dispensing button are made of an antimicrobial polymer) was carried out by pipetting 0.2 ml of the cell or conidiospore suspension on test pieces that were stored in a horizontal position throughout the experiments. After complete drying of the suspensions, the amount of colony forming units (cfu, a measure for viable cells) was determined after 4 hours and after 24 hours.

From key brands: Thermo Scientific Thermo Scientific Heraeus Thermo Scientific Pierce Sartorius Partec Santa Cruz
Our range includes: ULT freezers Cryogenic preservation tanks Software Cryo tubes Storage racks 2D barcode scanners Tube Picker 2D Cryo tubes and plates Decappers Cryo racks Mr Frosty From key brands: Thermo Scientific Revco Thermo Scientific ABgene Thermo Scientific Forma Thermo Scientific Nalgene Thermo Scientific Nunc Thermo Scientific Matrix Chart MVE
Mr. Frosty Provides the critical, repeatable -1C/minute cooling rate required for successful cell cryopreservation and recovery
Precise temperature control Accurate sample tracking Safe, secure storage
Streamlined harvesting Efficient, intuitive analysis Seamless data integration
Preventing Cell Culture Contamination with Copper CO2 Incubators
Imam El-Danasouri, DVM, Ph.D., HCLD. Director, California Reproductive Laboratories, 1700 California Street #570, San Francisco CA 94109 Daniel Schroen, Ph.D. Senior Application Scientist, Thermo Fisher Scientific
Introduction A CO2 incubator provides an excellent growth environment for cell cultures. However, the same warm, humid conditions can also sustain the growth of contaminating microorganisms. From easy-toclean design to external water reservoirs and heat decontamination cycles, Thermo Scientific Heracell CO2 incubators are proven to prevent and eliminate contamination(1-3). Antimicrobial action of copper Records from early civilizations demonstrate that copper can inhibit the growth of many different microorganisms. Reviews of modern literature (4) indicate that copper slows or stops growth of many organisms, including bacteria, fungi, algae and yeast. Copper ions bond to contaminants and then disrupt key proteins and processes that are critical to microbial life. For instance, the suppression of bacterial colonization by solid copper was recently demonstrated in Porton Down, England by the Centre for Applied Microbiology and Research (CAMR)(5). In that study, CAMR showed that copper piping reduces the growth of Legionella pneumophila, causitive agent of Legionaires Disease. This is the same testing facility that certifies many Thermo Scientific centrifuge rotors for biocontainment. CAMR also clearly documented that the Thermo Scientific Heracell ContraCon heat cycle effectively kills fungus and bacteria (2). There are many examples of copper acting as a microcide in everyday products, for example: Incorporated into cement or paint, it prevents bacterial and fungal growth (for example, in humid basements) It reduces bacteria and algae in cooling systems & towers Copper plumbing pipes reduce the threat from the bacteria Legionella pneumophila Brass (copper/zinc alloy) used in machining coolant filters removes bacteria and algae Copper-sulfate and -chelate aquacides control aquatic pests in ponds and municipal water supplies Copper-based pesticides control nematodes and fungi

seeding process used a novel bioreactor developed at the Politechnico di Milano, Italy, which provided the right environment for the cells to form the cartilaginous part of the trachea within four days of seeding. The graft was then lined with epithelial cells and transplanted into the patient, who responded very quickly to the new airway section, without any sign of rejection (no antibodies to the graft were found). Subsequent biopsies have shown that the new section is fully integrated with the existing airway and is fully supplied with blood vessels. She is now able to live life as if she had not been struck down with TB, a result that would never have been possible if her lung had been removed. Discussion Stem cell based-therapies have promised huge changes in the treatments of many diseases and disorders, but much research is still required to ensure safety and consistency before they can be applied more extensively. Prof Hollander and his colleagues at the Department of Cellular & Molecular Medicine at the University of Bristol have been investigating the fundamental principles governing the differentiation of bone marrow stem cells into chondrocytes the source of cartilage. Through this research they aim to further improve the processes used to generate chondrocyte-based autografts, which have already started to prove their value in the treatment of cartilage damage. Throughout their pioneering research, Professor Hollanders team has come to rely on the dependability and functionality of a broad array of standard and advanced laboratory equipment specifically designed to provide the highest quality and reliability in the cell biology laboratory. As a result of their dedicated work, Professor Hollanders team was able to take part in the amazing feat of the first ever bio-engineered tracheal graft. Their work has enabled this patient to regain an amazing quality of life following a life-threatening condition while increasing the drive among researchers and clinicians to more expansive use of stem cell based therapies.

separations are also regularly performed in ultracentrifuges and, more recently, a number of new nanotechnology applications have appeared. Low-speed centrifuges These large-capacity centrifuges have fairly specific applications due to the maximum RCF of approximately 7000 g. The most common lowspeed application is the separation of whole cells, for example, separating red blood cells and platelets from whole blood. The second most common application is the whole cell harvest step in the processing of large volumes of cultures from bioreactors in the bioprocessing and pharmaceutical industries. Lowspeed centrifuges offer a basic set of rotors that support volumes ranging from 1.5mL (with adapters) up to 2000mL. Choosing the right benchtop centrifuge Benchtop centrifuges are available in a wide variety of platforms designed for different application requirements, including general-purpose benchtops, microcentrifuges, small clinical centrifuges, cell washers, and high-speed benchtop centrifuges. General-purpose benchtop centrifuges These workhorse units (Figure 2) are the most common type of centrifuge found in the laboratory. Their versatility makes them extremely practical: They offer a wide range of rotor types, volumes, and speeds within a single unit to meet the demands of many common protocols. Generalpurpose centrifuges are typically used for tissue culture, DNA/RNA research, cell harvesting, subcellular separations, protein work, and many other applications. Most generalpurpose units can be equipped with a broad range of swinging-bucket and fixed-angle rotors, making them an excellent fit in a multiuser
environment where floor space is an issue and RCFs of up to 24,000 g are sufficient. Microcentrifuges Like the general-purpose benchtop models, microcentrifuges (Figure 3) are a necessity in every laboratory. These compact units provide RCFs of up to 21,000 g, which is sufficient for the most common microvolume applications such as plasmid, DNA/ RNA work, and mini-prep kits. Designed to spin up to 2mL volumes, microcentrifuges are typically equipped with rotors that accept commonly used 0.2-mL PCR tubes and 1.5-mL/2.0-mL disposable micro- centrifuge tubes and filters. Clinical benchtop centrifuges These compact models are designed for use in hospitals and clinics that require a low-throughput unit to spin blood collection tubes and urine samples at very low speeds for diagnostic examination. Most of these centrifuges spin at RCFs at or below 3000 g. The volume supported by a typical clinical benchtop unit ranges from 3-mL up to 15-mL tubes, with throughput ranging from 4 to 28 tubes per run, depending on the tube size and unit selected. Cell washers These specialpurpose centrifuges support very specific applications in the clinical and medical industry: washing away cellular debris, extraneous proteins, and other constituents of donor blood from red blood cells. The washed red blood cells are used for tests such as crossmatching prior to blood transfusion. Cell washers spin at RCFs at or below 1500 g and support 3-mL and 5-mL culture tubes. High-speed benchtop centrifuges These powerful and compact units offer g-forces close to that of a floormodel superspeed centrifuge, but with a limited set of rotors. The most

common rotors and applications for this type of centrifuge include a low-speed, high-volume swing-out rotor for a whole cell harvest step; a high-speed, midvolume (i.e., 5015 mL) fixed- angle rotor for a subcellular pelleting step; or a high-speed, low-volume (1.5-mL/2.0-mL) fixedangle rotor for certain DNA/RNA applications. The average maximum RCF for a high-speed benchtop is 50,000 g, with a volume range from 1.5 mL to 200 mL over a range of swinging-bucket and fixed-angle rotors. Understanding the different types of centrifuge platforms and the applications they support is a good first step in the selection process. Once you have identified your application needs and the appropriate centrifuge model, your centrifuge supplier should be able to assist you in configuring the best system (centrifuge, rotor, and consumables) to meet your specific requirements. For further information please contact: Dhru Patel (AU) dhru.patel@thermofisher.com or Ph: +4522 Kris Baker (NZ) kris.baker@thermofisher.com Ph: +980 6763
Figure 2 General-purpose benchtop centrifuges offer versatility and convenience, and can be equipped many ways to support tissue culture, DNA/RNA research, cell harvesting, subcellular separations, and many other applications.
Figure 3 Microcentrifuges are a necessity in every laboratory for everyday microvolume applications such as plasmid, DNA and RNA work, and miniprep kits.
The next generation in Automated liquid handling
The new Thermo Scientific Versette automated liquid handler
Compatible with 19 interchangeable, RFID-tagged pipetting heads, the Versette provides exceptional pipetting versatility for a broad range of applications that require single to 384 channel automated pipetting. To further optimize performance, the single, 8 and 12-channel pipetting heads utilize the Thermo Scientific ClipTips, which securely seal to the pipette head. The unique clip design requires minimal insertion and ejection force, decreasing wear and tear and increasing the life of the instrument and pipetting heads. The 96 and 384 channel pipetting heads use the Thermo Scientific D.A.R.T.s tips, which seal to ensure accurate and precise pipetting across all channels. The Versette platform is packed full of features, it offers two stage capacity options. These easy to swap 2 and 6 position stages provide flexibility for both stand-alone and robot-friendly use.
With a compact footprint, further facilitated by the unique dual-level design of the 6 position stage, the Versette can be placed exactly where it is needed, making it ideal for use on any benchtop or in an enclosure. Simple and complex pipetting procedures can be programmed quickly and easily via the intuitive onboard LCD user interface or the Thermo Scientific ControlMate software. This advanced instrument control, combined with precision and versatility, makes the Thermo Scientific Versette automated liquid handling platform ideal whether transitioning from handheld pipetting or establishing an integrated liquid handling system. For more information visit www.thermoscientific.com/versette or call Mika Mitropoulos (AU) on Mika.Mitropoulos@thermofisher.com Kris Baker (NZ) +kris.baker@thermofisher.com

La test ne Ne

When is a L not a L?
Have you ever picked up your pipette and thought Is this accurate?
Pipettes are used to measure and transfer liquids; a simple enough processes until you really think about it. What if it isnt reliably aspirating the correct amount of liquid? What will this do to your results? Can you rely on the results you are getting if your pipette is not calibrated? The answer isno.
The pipette is one of the most commonly used instruments in labs everywhere. Many pipettes are shared; they are used with varying liquids and are treated differently by different users. Pipettes work by creating an air gap to aspirate and then dispense liquid. The air gap is proportional to the amount of liquid that is being dispensed and it can be affected by many different factors. Usage over time, damage and general wear can have a major impact on dispensing accuracy and precision. If your pipette has not been checked and calibrated, the quality of the results obtained is questionable. It may not seem like a major factor at the time, but repeated incorrect dispensing can have a cumulative impact on results and can lead to major errors or even test failures. Next time you use a pipette, think about the quality of the results you want to achieve and whether the pipette in your hand is going to deliver.
Imagine the peace of mind that comes from knowing that all your valuable samples are being dispensed reliably every time you pick up your pipette. At Thermo Fisher Scientific we know that selecting the right pipette calibration service is important for the success and efficiency of your laboratory. We also know that each lab can vary considerably and thats why we have tailored our pipette service centre to meet your diverse needs.
We let you choose the services you want
Choose between 3 different modules for your pipettes:
Benefits of our NATA accredited pipette calibration centre are: Nata certified laboratory + equipment Accredited for all pipette calibrations from volumes 0.2L to 60mL 5 and 6 place balances as per AS2162.2-1998 Speedy turnaround times Loan pipettes (contract customers) We are the manufacturer of the Finnpipettes, therefore easier access to replacement pipettes/ warranty claims plus original and cost effective spare parts Relationships with all the other pipette manufacturers with access to their spare parts and warranties For further information email : ServiceAU@thermofisher.com 1. Maintenance & Calibration Internal & external inspection, cleaning and lubrication Replacement of seal/o-ring if necessary NATA calibration certificate Calibration sticker affixed to pipette to provide a suggested date for next calibration/service 2. Training & Education Over our many years of experience, we have developed first rate user training methods to ensure your staff correctly use and maintain their pipettes Our training workshops include correct pipetting techniques, maintenance, servicing and calibration of pipettes Workshops also cover AS/ISO standards, equipment recommendations and general requirements for setting up in-house calibration services Onsite or depot training options are available

CONTAINED SERVICE COSTS

R E PA I R & I N SP E C T I O N
I N STA LLAT I O N WA R R AN T Y E X T E N S I ON I N STALLAT I O N

TRAINING

MA IN T E N A N C E & C ALI B R AT I O N

EMERGENCY R E S P ON S E

Repair & Inspection Internal & external inspection, cleaning and lubrication Replacement of seal/o-ring if necessary Replacement of worn/broken parts Free of charge repair evaluation economical or uneconomical to repair? NATA calibration certificate Calibration sticker affixed to pipette to provide a suggested date for next calibration/service 3.
Chart MVE for Ultra Cold Storage
Thermo Fisher Scientific is proud to announce it is now the distributor of choice for Chart MVE vacuum insulated products and liquid nitrogen freezers.
Excellence & Innovation Chart-MVE is the worlds leading manufacturer of vacuum insulated products and cryogenic systems. More than forty years ago, we set the standard for storage of biological materials at low temperatures. Today, we continue to exceed these standards. Industries from around the world look to Chart-MVE for excellence and innovation. Our solutions empower industries to better utilize cryogenic technology. In this manner, Chart-MVE continues to make a vital contribution in todays biomedical industry. Chart-MVE has the solution for all of your cryogenic storage needs. We offer the broadest range of storage capacities for your biological products, with the most advanced vacuum technology available today. Chart-MVE is the market leader in the manufacturing of Bulk Storage, Liquid Cylinder and Vacuum Insulated Pipe products. Chart-MVE applied this knowledge to the development and creation of Turn Key liquid nitrogen supply systems that can provide your freezer with the most economical use of liquid nitrogen and the best return on your storage investment. Every Chart-MVE freezer is designed for optimum vacuum performance for the duration of its use. Chart-MVE freezers are engineered to hold and maintain specific temperatures, whether samples are in liquid or vapour. Chart-MVE offers the widest range of storage capacities and storage options (from -125C to -196C) to suit your biological product needs. By choosing Chart-MVE, you are installing a secure and viable environment, free of the noise and heat created by mechanical refrigeration systems. Chart-MVE products meet worldwide standards of excellence such as CE, MDD, UL, IATA, TGA, and ISO 9001. Factory tested to ensure reliability in the field, Chart-MVE vessels are backed by one of the strongest and longest warranties in the industry. Product Warranties Standard two (2) year warranty on all equipment. Three (3) year vacuum warranty on CryoSystem Series, Doble Series and Vapor Shippers. Five (5) year vacuum warranty on Stainless Steel Freezers, XC/SC Aluminum Units, and LAB units. Static evaporation rate and static holding times are nominal. Actual rate and holding time will be affected by the nature of container use, atmospheric conditions, and manufacturing tolerances.

Pass it on If you have enjoyed reading BioInnovation please pass it on to a colleague. They may enjoy reading the magazine and could learn something that could move their research forward.
November 14-18 November 19 November 23-24 December 2-3
Sign up for Bio-Innovation If you are new to Bio-Innovation and have received this copy from a colleague but would like your own, why not register for your own copy email: Bio-Innovation@thermofisher.com and guarantee that you are one of the first to receive the magazine when it is published? Publish in Bio-Innovation If youve used our products in an application and you feel others would benefit from the information generated, why not share it with the wider scientific community? Send your ideas or abstracts to us at Bio-Innovation@thermofisher.com Tell us what you think We would like to get your feedback on the content of the magazine so that we can improve it and increase your reading enjoyment. Send us your comments or suggestions for improvement to Bio-Innovation@thermofisher.com

December 3 December 5-9

February 3-15
As the world leader in serving science we are uniquely positioned to combine our unrivalled depth of product, application & service expertise with our extensive range of Scientific, Healthcare, Environmental & Industrial Process products to provide tailored solutions for each & every customer.
Photo of cardiomyocytes co-cultured with fibroblasts on HyStem-C : The photo was provided courtesy of Adam Engler, Stem Cell Biology and Bioengineering Laboratory, Department of Bioengineering, University of California, San Diego
Australia: For customer service, call 1300-735-292 Visit us online at: www.thermofisher.com.au New Zealand: For customer service, call 0800-933-966 Visit us online at: www.thermofisher.co.nz
2010 Thermo Fisher Scientific Inc. All rights reserved.