Development 104, 601-608 (1988) Printed in Great Britain The Company of Biologists Limited 1988
Pioneer neurones use basal lamina as a substratum for outgrowth in the embryonic grasshopper limb
HILARY ANDERSON and RICHARD P. TUCKER1
Department of Zoology, University of California, Davis, California 95616, USA
Present address: Friedrich Miescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland
Summary During axonogenesis, contacts made by the growth cone with its substratum are important in guiding the direction of neurone outgrowth. This study examines the contacts made by the growth cones of pioneer neurones in the embryonic grasshopper limb. Individual pioneer neurones at different stages of development were injected with horseradish peroxidase and the contacts made by the filopodia at the tip of their growth cones were examined by electron microscopy. Filopodia made few contacts with mesodermal cells, some contacts with ectodermal cells and very frequent contacts with basal lamina underlying the ectoderm. Components of the basal lamina may therefore play a role in guiding pioneer axon outgrowth.
Key words: pioneer neurones, basal lamina, growth cones, neurone substratum, grasshopper embryo.

Introduction

How do neurones navigate through embryonic tissue in precise patterns to find their appropriate targets? Interactions between the growth cone at the growing tip of an axon and its substratum clearly affect the direction of outgrowth of neurones growing in culture (Letourneau, 1975a; Collins & Garrett, 1980; Adler & Varon, 1981; Collins & Lee, 1984) and it seems likely that similar interactions are important in the embryo. However, little is known about the nature of the substrata used by growth cones in the embryo. Here we address this question in a simple system, the peripheral nervous system of the grasshopper embryo. At 30% of embryonic development, the limb buds are simple structures consisting of hollow tubes of ectoderm containing scattered mesodermal cells. At the tip of each limb bud, an ectodermal cell enlarges and divides to give rise to a pair of neurones which sink inward to lie beneath the ectoderm. These neurones are the Tibia 1 (Til) neurones; their cell bodies will eventually lie in the tibia of the fully formed limb (Keshishian & Bentley, 1983). They are most commonly referred to as pioneer neurones since they are the first neurones to appear within the limbs and form the first axon pathway between the limb and
the CNS (Bate, 1976). This pathway has a characteristic zigzag shape (Fig. 1) formed as the pioneers extend along the inner surface of the limbs, growing first proximally, then turning to grow circumferentially to the opposite side of the limb, and finally turning and growing proximally again to reach the CNS at about 35% of development (Bate, 1976). Shortly after the pioneer neurones begin their outgrowth, other neurones appear within the limb. These are the Femur 1 (Fel), Trochanter 1 (Trl), and pair of Coxa 1 (Cxi) neurones (Fig. 1; nomenclature according to Caudy & Bentley, 1986) whose axons will later fasciculate with those of the pioneer neurones and grow to the CNS. The navigational cues used by the pioneer neurones are intrinsic to the limb bud; a normal pioneer pathway is formed in limb buds cultured in isolation from the rest of the embryo (Lefcort & Bentley, 1987). These cues could be provided by the ectoderm, the mesoderm or the immature neurones. The first proposed source of cues was the immature neurones, which are frequently contacted by the pioneer neurones (Ho & Goodman, 1982; Keshishian & Bentley, 1983). They are found at particular points along the pioneer pathway (Fig. 1): the Fel neurone midway along the initial proximal section, the Trl

H. Anderson and R. P. Tucker
microscope slide. The embryo was held in place with fine hairs anchored in the silicon grease.
neurone near the first turn, and the Cxi pair of neurones near the second turn (Fig. 1). It was proposed that these immature neurones form a chain of 'guideposts' spaced about 50 /mi apart, and that the pioneer growth cones grow from one 'guidepost' cell to the next with which their long (50-100/xm) filopodia were in contact (Bentley & Keshishian, 1982). The zigzag distribution of these immature neurones would therefore determine the pathway taken by the pioneers (Bentley & Keshishian, 1982). Elimination of the Cxi pair of neurones in cultured embryos does indeed result in the pioneer neurones taking abnormal routes at the end of their pathway (Bentley & Caudy, 1983). However, there is no compelling evidence that the Fel and Trl neurones also provide critical guidance cues. In fact, the Fel neurone usually appears only after the pioneer growth cones have passed by (Ho & Goodman, 1982; Berlot & Goodman, 1984). In addition, one or both pioneers frequently fail to contact the Fel or Trl neurones (Ho & Goodman, 1982; Caudy & Bentley, 1986), yet form a normal pathway. Furthermore, the Fel and Trl neurones are delayed in development in certain lines of embryos (Caudy & Bentley, 1986), and in cultured limbs (Lefcort & Bentley, 1987), and have been surgically removed from limbs (Lefcort & Bentley, 1987), yet the pioneer neurones form a normal pathway in their absence. Much of the pathway can therefore be navigated using cues other than immature neurones. The need to identify these other cues prompted us to carry out this study. We have examined the substrata used by the pioneer neurones during different stages of their outgrowth by injecting individual pioneer neurones with the marker horseradish peroxidase and examining by transmission electron microscopy the contacts made by their growth cones and associated filopodia. We find that a basal lamina underlying the limb ectoderm is used extensively as a substratum and we propose that interactions between pioneer neurone growth cones and components of this basal lamina may be important in directing pioneer neurone outgrowth.

Materials and methods

Intracellular injection of horseradish peroxidase
The embryo was viewed with Nomarski optics through a Leitz x50 saltwater immersion lens on a Zeiss Standard microscope. Individual pioneer neurones were penetrated with microelectrodes filled with 4 % horseradish peroxidase (HRP; Sigma Type VI) in M - K C 1 , 0-2M-THS buffer, pH7-4. Successful penetrations were indicated by stable resting potentials recorded through the microelectrode. HRP was injected into the cell body with about 2nA depolarizing current for about 2 min.
Processing for electron microscopy
Injected embryos were left for 30 min at room temperature to allow the HRP to diffuse throughout the neurone. They were then fixed for 1 h on ice in 2 % glutaraldehyde, 0-5 % paraformaldehyde in 0-lM-sodium cacodylate buffer, thoroughly rinsed in 0-lM-cacodylate buffer, rinsed for 5 min in 0-05M-Tris buffer, pH7-6, and then reacted in 0-5% 3,3 diaminobenzidine (ICN Biomedicals) in 0-05 MTris buffer, pH7-6 containing 0-006% hydrogen peroxide. The reaction was monitored under a dissecting microscope and stopped when appropriate by transferring the embryo to a large volume of Tris buffer. The embryos were temporarily mounted on a depression slide in buffer and the injected neurones were drawn using a camera lucida. The embryos were then postfixed for 2h on ice in 2% OsO4 in O'lM-cacodylate buffer, rinsed in distilled water, dehydrated and embedded in Araldite.

Electron microscopy

Specimens were oriented to yield cross-sections through the limb containing the injected neurone and sectioned at 8 fm\ on a Sorvall MT-2 microtome. The sections were dried on to siliconized microscope slides and observed with a Zeiss Standard microscope. Appropriate sections were photographed or drawn, re-embedded and thin sections were cut with a diamond knife on a Reichert OmU3 ultramicrotome. Thin sections were stained in 2 % aqueous uranyl acetate and Venable's lead citrate, and observed at 60 kV with a Philips 410 transmission electron microscope. Results

Embryos

Eggs were collected daily from a colony of Schistocerca americana and kept in an incubator at 32 C, at which temperature hatching (100 % of development) took place about 20 days after laying (0 % of development). Embryos that had completed 30 % to 35 % of development were dissected from their egg cases in saline (Shankland et al. 1982) containing 12 gl" 1 sucrose. The amniotic membrane was removed, the dorsal closure opened and the embryo placed in a pool of saline within a silicon grease well on a
Fig. 1 shows the distinctive pathway formed by the pioneer neurones within the embryonic grasshopper limb. Three HRP-filled neurones were selected for detailed study of which surfaces pioneer neurone growth cones contact. Each had reached a different point along the pathway, indicated by the numbers I, II and III on Fig. 1, which are the numbers also used to designate the individual neurones. Examination of an additional six neurones at similar stages of development supported these observations. The pioneer neurones, when viewed in the light microscope, do not display a clearly defined growth cone such as those displayed by some vertebrate
Basal lamina is a substratum for pioneer growth cones
Fig. 1. Camera-lucida drawing of the complete pathway of the Til pioneer neurones in a 35% metathoracic limb. Til, Fel, Trl, Cxi indicate the cell bodies of the Tibia 1 (pair), Femur 1, Trochanter 1, and Coxa 1 (pair) neurones, respectively. The arrowheads labelled I, II and III indicate the approximate extents of the three neurones described in detail in this study. The presumptive limb segments are indicated by dotted lines, and are, the coxa (Cx), trochanter (Tr), femur (Fe), tibia (Ti), and tarsus (Ta). Bar, 0-1 mm. neurones in which the tip of the axon is considerably enlarged and extended into many veil-like lamellipodia and long, thin filopodia (e.g. Mason, 1985). Rather, the pioneer axons extend processes all along their lengths, with a greater profusion in the distal region which is only slightly enlarged and has few lamellipodia. For our purposes, therefore, we used the term growth cone to designate the tip of the growing axon. Ultrastructural observation confirmed that this was the region containing the usual complement of organelles associated with growth cones. In this study, we wished to examine filopodial contacts that might be involved in axon navigation and, therefore, largely restricted our observations to those filopodia extending from the growth cone, where directional changes in growth take place. We, therefore, ignored the very many filopodia that extend from all over the axon behind the growth cone. While some of these lateral filopodia could be remnants offilopodiaonce formed at the growth cone and now left behind on the axon (Bray & Chapman, 1985), some are formed directly from the axon (Keshishian, 1980). For example, pioneer axons that have already completed their pathway show subsequent expansion or flattening of the axon and extension of many filopodia in the region of the developing border between the tibia and femur segments (Caudy & Bentley, 1986). Filopodia formed in this position at this stage clearly serve functions other than influencing the direction of axon outgrowth. Neurone I The cell bodies of Neurone I and its unlabelled sibling

appear in the light microscope as two slightly elongated cells closely apposed to the ectoderm about two cell diameters from the tip (Fig. 2A); the limb has continued to elongate since they were displaced from the ectoderm at the tip of the limb. They have extended axons as far as the distal part of the presumptive femur (Fig. 2A). The cell bodies make some contact with the ectoderm but are mostly separated from it by basal lamina (Fig. 2B,C). On the lumen side, the cell bodies may have patches of basal lamina material associated with their surface, but may also be in contact with mesodermal cells (Fig. 2B). The tips of the pioneer axons lie between the ectoderm and the basal lamina (Fig. 2D). The pioneers do not have extensive direct contact with the ectoderm (Fig. 2E) but their luminal surfaces are very tightly apposed to the basal lamina (Fig. 2E). The filopodia extending from the growth cones may be found between the ectoderm and its basal lamina (Fig. 2F), but are most frequently associated with basal lamina alone (Fig. 2G,H). Neurone II Neurone II has extended its axon into the proximal region of the presumptive femur, almost as far as the trochanter border (Fig. 3A). The cell bodies are separated from the ectoderm by one or more layers of basal lamina (Fig. 3B). The growth cone is also isolated from the ectoderm; it is sandwiched between layers of basal lamina (Fig. 3C). The filopodia from the tip of the growth cone also contact only basal lamina (Fig. 3D,E). No examples of filopodia touching mesodermal cells or ectodermal cells were found in this region. Neurone III Neurone III has extended its growth cone the full length of the first proximal section of the pathway to the border between the coxa and trochanter, and has turned around the circumference of the limb, reaching about halfway towards the opposite side (Fig. 4A). The relationship between the growth cone and filopodia and the surrounding tissues is the same as for Neurone II - they are in contact with basal lamina on both sides (Fig. 4B,C). In the distalmost part of the presumptive femur, the pioneer axon is closely apposed to the ectoderm and has many short processes which extend along or between the ectoderm cells (Fig. 4D-G). Such an arrangement was not seen in preparations in which only the axon tip had reached this area (e.g. Fig. 2F-H). Nor was this degree of apposition seen in other regions of the axon. This is a region where additional lateral branches and filopodia were ob-
Fig. 2. Neurone I. (A) Camera-lucida drawing showing the extent of outgrowth of Neurone I. (B) The cell bodies of Neurone I, which was injected with HRP, and its uninjected sibling. (C) Higher magnification of B showing basal lamina between the pioneer cell bodies and the ectoderm. (D) The growth cones of neurone I and its sibling and their arrangement within the limb. (E) Higher magnification of D showing contacts between the growth cones and the ectoderm and basal lamina. (F-H) Filopodia extending from the growth cone of Neurone I. Arrows indicate basal lamina; large arrowhead, the HRP-injected neurone; small arrowhead, the uninjected neurone; stars, filopodia; e, ectoderm; m, mesoderm; lu, lumen of the limb. Bar, 0-1 mm (A), 5/mi (B,D), 1/rni (C,E,F,G,H).

served in wholemount preparations (Caudy & Bentley, 1986). On its luminal side, the axon is closely apposed to several layers of basal lamina (Fig. 4D).

Discussion

The pioneer neurones of the embryonic grasshopper limb grow in a characteristic zigzag trajectory through
the limb to the CNS (Fig. 1). What cues are used by the pioneers during their outgrowth? We observed very few contacts between labelled pioneer neurones and mesodermal cells at any stage of the pathway. This is probably because mesoderm and ectoderm are separated throughout most of the pathway by a layer of extracellular matrix, the basal lamina. It seems unlikely therefore that mesodermal
Fig. 3. Neurone II. (A) Camera-lucida drawing showing the extent of outgrowth of Neurone II. (B-E) Electron micrographs of the cell body (B), growth cone (C), and growth cone filopodia (D,E). Symbols and abbreviations as in Fig. 2. Bar, 0-1 mm (A), l^m (B-E). cells play a significant role in guiding the pioneer neurones at any stage of the pathway. Experimental evidence that this is the case has been obtained by observing normal pathways formed by pioneer neurones in isolated cultured limbs from which the mesoderm had been removed (Lefcort & Bentley, 1987). Growth cones within the distal half of the proximal pathway were in contact with ectodermal cell surfaces as well as with basal lamina (Fig. 2D,E). Filopodia extending from growth cones at this stage of development were rarely in contact with ectodermal cells (Fig. 2F), but were commonly in contact with basal lamina (Fig. 2G,H)- Growth cones within the proximal part of the proximal pathway and the circumferential pathway were usually separated from the ectoderm and were sandwiched between layers of basal lamina (Figs 3C, 4B). Filopodia from growth cones at this stage of development were usually in contact with basal lamina (Figs 3D,E> 4C). As the axons elongate, filopodia emerge laterally behind the growth cones and extend into the interstitial spaces between the adjacent ectodermal cells (Keshishian, 1980). Thus along the proximal pathway later in development far more extensive contacts with ectodermal cells are formed by filopodia (e.g. in the distalmost femur, Fig. 4D), but these are lateral filopodia. Such contacts are probably not involved in growth cone orientation, but could be a passive consequence of changed surface properties of the developing ectoderm with which they are in contact (Caudy & Bentley, 1986), or could perhaps serve temporarily to anchor the axon to the ectoderm, or to mediate other interactions between pioneers and
Fig. 4. Neurone III. (A) Camera-lucida drawing showing the extent of outgrowth of Neurone III. (B-G) Electron micrographs of the growth cone (B) and its associated filopodia (C), and of the axon (D) and axonal filopodia (E-G) in the region marked by a box in A. Symbols and abbreviations as in Fig. 2. Bar, 0-1 mm (A), ljan (B-C), 5 f.tm (D), 0-5 jim (E-G).

ectodermal cells. Previous studies have emphasized the possible role of ectodermal cell surface cues in guiding pioneer neurone outgrowth. Berlot & Goodman (1984) have suggested that the ectoderm might provide a polarity cue to guide the proximal outgrowth of pioneers. They observed that in the antenna the epithelial cells express an intrinsic polarity in their shape. The more distal cells overlap the luminal surface of the more proximal cells; this is particularly prominent near the antennal tip where the neurones make their initial polarity decision. We have not been able to demonstrate such an arrangement in the limb in this or a
related study (Anderson & Tucker, in preparation). Caudy & Bentley (1986) have suggested that ectodermal cell surface cues are important in maintaining proximal pioneer outgrowth. They proposed that the affinity of the ectoderm for pioneer growth cones is not uniform throughout the limb, but increases proximally within segments with a peak at the segment boundary, and that increasing ectodermal affinity might act as a cue for growth cones to grow proximally. Observations that support the first part of their proposal are: (1) in clutches of embryos in which pioneer neurones develop at an unusually early stage of limb development (selected so that the pioneers
Basal lamina is a substratum for pioneer growth cones complete much of their pathway without the complicating presence of putative guidepost cells), pioneer neurone branching is increased in the proximal femur over the distal femur; and (2) in normal embryos, more lateral branches and filopodia are extended from the growth cone as it crosses the tibia-femur segment boundary than when it crosses the regions just proximal or just distal to the boundary. Branching and filopodium extension are known to be increased in vertebrate neurones cultured on artificial substrata to which they adhere more strongly (Letourneau, 1975a), or in the presence of higher concentrations of NGF (Connolly et al. 1985). Assuming that these correlations also exist for neurones growing within the grasshopper embryo, they argue that pioneer growth cones adhere more strongly to, or have a higher affinity for, the tibia-femur segment boundary and the proximal femur. In support of the second part of their proposal, they cite evidence that vertebrate neurones in culture orient to remain on substrates of higher adhesivity (Hammarback et al. 1985; Letourneau, 19756) or with a higher density of surface-bound NGF (Gunderson, 1985). Thus, when presented with ectodermal regions of differing affinity for growth cones, the pioneers will orient towards the proximally increasing affinity regions. Ectodermal cues have also been proposed to be important in guiding pioneers along the circumferential part of the pathway. The pioneer neurones generally make their first turn when they encounter the prospective trochanter-coxa segment boundary, which becomes morphologically distinct at a later stage of development when the ectodermal cells in this area change shape and invaginate a little (Caudy & Bentley, 1987). The pioneers extend along this boundary until their filopodia contact the Cxi cells towards which their growth cones then grow. In some 20 % of embryos, the pioneers grow directly from the proximal pathway to the Cxi cells (Caudy & Bentley, 1987). The abrupt growth cone reorientation at the trochanter-coxa boundary could be due to either a high affinity for the segment boundary itself or an aversion to extension over coxa ectoderm, or both (Caudy & Bentley, 1987). In the present EM study, growth cones and their filopodia had more extensive contact with basal lamina than with ectodermal cell surfaces. It is possible that the increased affinity of proximal limb regions for neurones proposed by Caudy & Bentley (1986) and the high affinity of the trochanter-coxa segment boundary or the low affinity of the coxa segment for neurones proposed by Caudy & Bentley (1987) actually lies in the basal lamina beneath the ectoderm rather than in the ectoderm itself. A descriptive study such as that presented here

cannot resolve the issue of which factors are responsible for directing pioneer axon outgrowth in the embryonic grasshopper limb. That requires experimentation. However, in the absence of any compelling evidence in favour of other cues, we suggest that components of the basal lamina could provide a generally preferred substratum for axon outgrowth and could also provide directional cues for growth cone navigation. Very little is known about the organization and composition of insect basal lamina or its interactions with neurones. The present study suggests that these topics might be worthy of further investigation. This work was supported by grant NS 21355 to H. A. from the National Institutes of Health. We thank Drs Michel
Roberge and Vernon French for critical reading of the manuscript. References
ADLER, R. & VARON, S. (1981). Neuritic guidance of
polyornithine-attached materials of ganglionic origin.

DevlBiol. 81, 1-11.

BATE, C M. (1976). Pioneer neurones in an insect embryo. Nature, Lond. 260, 54-56. BENTLEY, D. & CAUDY, M. (1983). Pioneer axons lose directed growth after selective killing of guidepost cells. Nature, Lond. 304, 62-65. BENTLEY, D. & KESHISHIAN, H. (1982). Pathiinding by peripheral pioneer neurons in grasshoppers. Science 218, 1082-1088. BERLOT, J. & GOODMAN, C. S. (1984). Guidance of peripheral pioneer neurons in the grasshopper: adhesive hierarchy of epithelial and neuronal surfaces. Science 223, 493-4%. BRAY, D. & CHAPMAN, K. (1985). Analysis of microspike movements on the neuronal growth cone. J. Neurosci. 33, 3204-3213. CAUDY, M. & BENTLEY, D. (1986). Pioneer growth cone morphologies reveal proximal increases in substrate affinity within leg segments of grasshopper embryos. J. Neurosci. 6, 364-379. CAUDY, M. & BENTLEY, D. (1987). Pioneer growth cone behavior at a differentiating limb segment boundary in the grasshopper embryo. Devi Biol. 119, 454465. COLLINS, F. & GARRETT, J. E. (1980). Elongating nerve fibers are guided by a pathway of material released from embryonic nonneuronal cells. Proc. natn. Acad. Sci. U.S.A. 77, 6226-6228. COLLINS, F. & LEE, M. R. (1984). The spatial control of ganglionic neurite growth by the substrate-associated material from conditioned medium: an experimental model of haptotaxis. J. Neurosci. 4, 2823-2829. CONNOLLY, J. L., SEELEY, P. J. & GREENE, L. A. (1985). Regulation of growth cone morphology by nerve growth factor. J. Neurosci. res. 13, 183-198. GUNDERSEN, R. W. (1985). Sensory neurite growth cone guidance by substrate adsorbed nerve growth factor. /. Neurosci. Res. 13, 199-212.

119, 466-480. P. C. (1975a). Possible roles for cell-tosubstratum adhesion in neuronal morphogenesis. Devi Biol. 44, 77-91. LETOURNEAU, P. C. (1975b). Cell-to-substratum adhesion and guidance of axonal elongation. Devi Biol. 44, 92-101. MASON, C. A. (1985). Growing tips of embryonic cerebellar axons in vivo. J. Neurosci. Res. 13, 55-73. SHANKLAND, M., BENTLEY, D. & GOODMAN, C. S. (1982). Afferent innervation shapes the dendritic branching pattern of the medial giant interneuron in grasshopper embryos raised in culture. Devi Biol. 92, 507-520.

LETOURNEAU,

HAMMMARBACK, J. A., PALM, S. L., FURCHT, L. T. &
LETOURNEAU, P. C. (1985). Guidance of neurite outgrowth by pathways of substratum-adsorbed laminin. J. Neurosci. Res. 13, 213-220. Ho, R. K. & GOODMAN, C. S. (1982). Peripheral pathways are pioneered by an array of central and peripheral neurones in grasshopper embryos. Nature, Lond. 297, 404-406. KESHISHIAN, H. (1980). The origin and morphogenesis of pioneer neurons in the grasshopper metathoracic leg. Devi Biol. 80, 388-397. KESHISHIAN, H. & BENTLEY, D. (1983). Embryogenesis of peripheral nerve pathways in grasshopper legs. Devi Biol. 96, 89-102. LEFCORT, F. & BENTLEY, D. (1987). Pathfinding by pioneer neurons in isolated, opened and mesodermfree limb buds of embryonic grasshoppers. Devi Biol.
(Accepted 8 September 1988)

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Ref. No. 23 24

Part No. XF250-A-0104100A XF250-A-0104201A XF250-A-0104202A XF250-A-0104203A XF250-A-0104204A XF250-A-0104210B XF250-A-0104205A XF250-A-0104300A XF250-A-0104001A XF250-A-0104002A XF250-A-0104003A XF250-A-0104004A XF250-A-0104005A XF250-A-0104006A XF250-A-0104007B XF250-A-0104008 XF250-A-0104009A XF250-A-0104011A XF250-A-0104012A XF250-A-0104013A XF250-A-0104014A XF250-A-0104020 XF250-A-0114015A XF250-A-0104016B
Description Crank Shaft Component Piston Piston Pin 1st Piston Ring 2nd Piston Ring Oil Ring Component Clip Piston Pin Cam Chain Adjustor Guide, Cam Chain Gear Primary Drive Gear Oil Pump Drive Washer Lock M16 x 1 Nut Hex Bolt Rotor Setting Starter Clutch Gear Washer 12 Stud 2 x 14 Spring Hollow Oil Bolt Bolt M6 x 26 Washer 6 Oil Seal 22 x 31x 5 Plate Washer Pin Lock

Page 6 of 56

Figure E05

CAMSHAFT

Figure No. E5 E5 E5 E5 E5 E5 E5 E5 E5 E5 E5 E5 E5 E5 E5

Ref. No. 15

Part No. XF250-A-0105101A XF250-A-0105102A XF250-A-0105103A GB894.2-86 XF250-A-0105105A XF250-A-0105200A XF250-A-0105201A XF250-A-0105202A XF250-A-0105203A XF250-A-0105204A XF250-A-0105205A XF250-A-0105206A XF250-A-0105001A XF250-A-0105002A XF250-A-0105300A
Description Cam Shaft Bushing, Camshaft Pin, Dowel Bush Camshaft Circlip, External Washer Rocker Arm Assembly Camshaft Tower Shaft Valve Rocker Arm Spring Rocker Arm Side Arm Valve Rocker Screw Tappet Adjusting Nut, Tappet Adjusting Sprocket Cam M7 x 13 Bolt Chain Cam

Page 7 of 56

Figure E06

OIL PUMP

Figure No. E6 E6 E6 E6 E6 E6 E6 E6 E6 E6 E6 E6 E6 E6 E6
Part No. XF250-A-010600A XF250-A-0106001B XF250-A-0106002A XF250-A-0106003A XF250-A-0106004A XF250-A-0106005A XF250-A-0106006A XF250-A-0106007A XF250-A-0106010 XF250-A-0106110A XF250-A-0106102A XF250-A-0106008A XF250-A-0106009A GB308-77 GB5780-86
Description Oil Pump Assembly Oil Pump Body Oil Pump Cover Back Cover Shaft Oil Pump Gear Oil Pump Rotor Oil Pump Outer Rotor Oil Pump Inner Screw M5 x 7.5 Oil Filter Screen Oil Filter Holder Screen Holder Gasket Pin, Dowel Steel Ball 8 Bolt M5 x 20 Q-ZnD

Page 8 of 56

Figure E07

CLUTCH ASSEMBLY

Figure No. E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7 E7

Ref. No. 17

Part No. XF250-A-0107110A XF250-A-0107120A XF250-A-0107121A XF250-A-0107122A XF250-A-0107123A XF250-A-0107124A XF250-A-0107125A XF250-A-0107126A XF250-A-0107127A XF250-A-0107128A XF250-A-0107129A XF250-A-0107101A XF250-A-0107102A XF250-A-0107001A XF250-A-0107002A XF250-A-0107003A XF250-A-0107200A
Description Outer Component Clutch Centre Frame Component Center Frame Disk Clutch Friction Plate Clutch Pressure Plate Clutch Clutch Wave Ring 1 Clutch Wave Ring 2 Spring Clutch Bolt M6 x 22 Pressure Cover Guide Clutch Outer Washer Spline Washer Circular Nut Washer Thrust Pushing Bearing

Page 9 of 56

Figure E08

LEFT CRANKCASE COVER

Figure No. E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8 E8

Ref. No. 15 16

Part No. XF250-A-0108101A XF250-A-0108102A GB70-85 XF250-A-0108103A XF250-A-0108104A XF250-A-0108105A XF250-A-0108106A GB70-85 GB70-85 XF250-A-0108001A XF250-A-0108002A XF250-A-0108003A GB5787-86 XF250-A-0108004A XF250-A-0112002A XF250-A-0108006A

Description Cover Component Right Crankcase Clutch Lever Component Bearing HK 1210 Oil Seal 12 x 18 x 5 Pin, Clutch Lifter Receiver, Clutch Cable Gauge, Oil Level O-Ring 18 x 3 Spring, Clutch Lever Bolt M6 x 40 Bolt M6 x 50

Page 14 of 56

Figure E13

LEFT REAR CRANKCASE

Figure No. E13 E13 E13

Ref. No. 3

Part No. XF250-A-0113100B XF250-A-0113001 XF250-A-0113002
Description Left Rear Crankcase Cover Bolt M6 x 46 Bolt M6 x 60

Page 15 of 56

Figure E14 (a)

TRANSMISSION

Figure No. E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14

Ref. No. 19 20

Part No. XF250-A-0114101A XF250-A-0114102A XF250-A-0114103A XF250-A-0114104A XF250-A-0114105A XF250-A-0114106A XF250-A-0114107A XF250-A-0114108A XF250-A-0114201A XF250-A-0114202A XF250-A-0114203A XF250-A-0114204A XF250-A-0114205A XF250-A-0114206A XF250-A-0114207A XF250-A-0114208A XF250-A-0114209A XF250-A-0114401A XF250-A-0114402A GB5783-86
Description Mainshaft Component Gear Mainshaft Second 18T Gear Mainshaft Third 21T Gear Mainshaft Forth 24T Gear Mainshaft Fifth 32T Washer Spline Set Ring Washer Spline Countershaft Component Gear Countershaft First 37T Gear Countershaft Second 32T Gear Countershaft Third 28T Gear Countershaft Fourth 26T Gear Countershaft Fifth 21T Bush Washer Retaining Ring Sprocket Counter Shaft Plate Fixing Bolt M6 x 10/ZnD

Page 16 of 56

Figure E14 (b)
Figure No. E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14 E14

Ref. No. 33 34

Part No. XF250-A-0114300B XF250-A-0114500A XF250-A-0114501 XF250-A-0114502A XF250-A-0114503A XF250-A-0114001A XF250-A-0114002A XF250-A-0114003A XF250-A-0114004A XF250-A-0114005A XF250-A-0114006A XF250-A-0114007A XF250-A-0114601A XF250-A-0114602A
Description Spindle Component, Gearshift Stopper Component, Gearshift Drum Spring Shift Drum Stopper Washer 6 Screw M6 x 12 Fork Left Gearshift Fork Center Gearshift Fork Right Gearshift Shaft Gearshift Fork Guide Plate Drum Stopper Pin, Gearshift Drum Screw M6 x 20 Drum Gearshift Rotor Neutral Switch

Page 17 of 56

Figure F01

FRAME BODY

Figure No. F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1
Part No. GB6187-86 XF250-A-0205-001 XF250-A-0205-002 XF250-A-0205-003 GB5787-86 XF250-A-0205-000 GB6187-86 GB5786-86 XF250-A-AJ0200-001 XF250-A-0201-0020 XF250-AJ-0201-000 DD90E-1300-006 XF250-A-0200-001 XF250-A-0202-000 XF250-A-0202-001 XF250-A-0202-002 GB1014-86 XF250-A-5-0204-000 GB5787-86 XF250-A-0200-005 GB827-86 XF250-A-0205-005

Description Nut M6 x 1.25 Bush Oil Seal Washer Bolt M8 x 65 Lower Bracket, Engine Nut M8 Bolt M10 x 1.25 x 128 Bolt M10 x 1.25 x 198 Frame Component Frame Bolt M8 x 40 Right Plate Left Plate Washer Rubber Pressure Plank Rivet 5 x 8 Bracket Bolt M10x1.25x112 Nameplate Rivet 2x5 Washer 10

Page 18 of 56

Figure F01 (Continued)
Figure No. F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1 F1

Ref. No. 33

Part No. GB6170-86 XF250-A-2900-001 GB5787-86 GB5787-86 DD90E-1900-002 GB96-85 XF250-A-0202-003 XF250-A-0000-001 XF250-A-0201-050 XF250-AJ-0200-008 XF250-AJ0200-007 XF250-A-AJ-2-2901-000 GB5787-86
Description Nut M6 Protector Bolt M6 x 50 Molt M6 x 45 Nut M6 Washer 6 Bolt M6 x 12 Radiator Catch Tank Bracket Spacer 30 Spacer 8 Protector Bolt M6 x60

Page 19 of 56

Figure F02

HEADLIGHT ASSEMBLY

Figure No. F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2
Part No. XF250-A-8-0302-000 GB818-85 XF250-A-0301-011 XF250-A-0301-010 XF250-A-8-0302-001 XF250-A-0301-008 XF250-A-0301-006 XF250-A-0301-001 XF250-A-0301-005 XF250-A-0301-002 XF250-A-0301-003 XF250-A-0301-004 GB818-85 GB5787-86 XF250-A-0706-000 XF250-A-0301-004 GB6187-86
Description Headlight Assembly Bolt M5 x 12 Set Mat Light Combination Headlight Lens Headlight Rim Rubber Washer Shell Headlight Spacer Socket Assembly Bulb Headlight 12v35w Spring Bolt M4 x 40 Bolt M6 x 45 Frame Headlight Cover Nut M6 Self Locking Nut M8

Page 20 of 56

Figure F03

TURN LIGHT ASSEMBLY

Figure No. F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3 F3

Ref. No. 25 26

Part No. XF250-A-0302-000 GB845-85 XF250-A-0302-005 XF250-A-0302-004 XF250-A-0302-007 XF250-A-0302-003 XF250-A-0302-100 XF250-A-0302-001 XF250-A-0302-006 XF250-A-0303-000 XF250-A-0308-000 XF250-A-0308-001 XF250-A-03058-100 XF250-A-0308-002 XF250-A-0306.000 XF250-A-0308-003 XF250-A-0308-004 XF250-A-0308-200 XF250-A-0301-012 XF250-A-0304-000 XF250-A-0304-001 XF250-*A-1100.006 DD90E-2700-002 XF250-A-0301-004 XF250-A-0305-000 XF250-A-0307-000 XGF250-A-0305-005 XF250-A-0305-004
Description Left Turn Light, Front Self Tapping Bolt, Flange ST42 x 24-F-H Lens, Left Front (Right Rear) Blinker Bulb, Blinker Fr 12v10/3w Lamp Bulb 12v 21/5w Rubber Cushion Socket, Front Blinker Shell, Blinker Left Front (Right Rear) Phillips Bolt Rubber Socket, Blinker Left Front Right Turn Light, Rear Front Right (Rear Left) Lamp Shade Rear Lamp Frame And Wire Bolder Front Right (Rear Left) Lamp Shell Rubber Seat Of Back Steering Lamp Rubber Grommet Fixed Position Set Installing Plate R Cover Nut M8 Right Turn Light, Front Rubber Socket, Blinker Left Front Bolt M6x25 Washer, Front Fender Cover Nut M6 Left Turn Light, Rear Installing Plate I. Bulb, Blinker Rear Bulb, Blinker Rear

Description Screw M6 x 16 Advertisement Plate Hook Bolt M8 x 22 Stem Component, Steering Bolt M 6 x 12 Lock Head Card Hook Bearing Nut Top Cover Of Roller Bearing Steel Ball D4.763 Roller Bearing Ring Lower Frame Of Roller Bearing Sealing Block Ring Side Reflector Assembly Nut M5

Page 28 of 56

Figure F11

FRONT SHOCK ABSORBER

Figure No. F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11 F11
Part No. XF250-A-0700-007 XF250-A-0704-000 XF250-A-0703-003 XF250-A-0703-004 XF250-A-0703-005 XF250-A-0704-003 XF250-A-0703-006 XF250-A-0704-002 XF250-A-0703-007 XF250-A-0703-008 XF250-A-0703-009 XF250-A-0703-010 XF250-A-0703-011 XF250-A-0703-012 GB3452.1-88 XF250-A-0703-013 XF250-A-0704-001 XF250-A-0703-014 XF250-A-0703-015 XF250-A-0703-016 XF250-A-0703-017 XF250-A-0703-000 XF250-A-0703-001 XF250-A-0703-002
Description Screw M8 x 25 Front Shock Absorber, Right Screw M8 x 25 Washer Self Locking Nut Bottom Fork Cap Double Head Bolt Damping Tube, Right Front Turn Block Ring Framework Sealing Washer Block Ring Dust Seal Nut Cover O-Ring Damping Spring I Damping Post, Right Damping Spring II Set Piston Assembly Stop Cover Front Shock Absorber, Left Damping Tube, Left Front Damping Post, Left

Page 29 of 56

Figure F12

FRONT WHEEL

Figure No. F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12 F12
Part No. XF250-A-0800-001 XF250-A-0800-002 XF250-A-0800-003 XF250-A-0800-013 GB278-88 XF250-A-0800-005 XF250-A-0801-000 XF250-A-0800-012 XF250-A-0800.011 XF250-A-0804-001 XF250-A-0804-002 XF250-A-0804-003 XF250-A-0804-004 XF250-A-0806-000 XF250-A-1000-005 XF250-A-0804-100
Description Front Wheel Axle Disk, Front Brake Hex Bolt Inner M8 x 24 Bushing II Wheel Bearing 60302 Framework Oil Seal 22x42x7 Rim, Front Wheel Front Tire Axel Set Speedometer Drive Plate Framework Oil Seal 40x50x5 Wheel Gear Washer Box, Speedometer Gear Spirit Nut Joint Of Speedometer Gear Set

Page 30 of 56

Figure F13

FRONT BRAKE

Figure No. F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13

Ref. No. 27

Part No. XF250-A-0802-000 XF250-A-0802-200 XF250-A-0802-001 XF250-A0802-002 XF250-A-0802-003 XF250-A-0802-100 XF250-A-0802-004 XF250-A-0802-005 XF250-A-0802-006 XF250-A-0802-007 XF250-A-0802-008 XF250-A-0802-009 XF250-A-0802-010 XF250-A-0802-011 XF250-A-0802-012 XF250-A-0802-013 XF250-A-0802-020 XF250-A-0802-016 XF250-A-0802-017 XF250-A-0802-018 XF250-A-0802-019 GB5787-86 XF250-A-0803-000 XF250-A-0803-001 XF250-A-0803-002 XF250-A-0803-003 XF250-A-0803-004
Description Pump, Front Brake Pipeline Module Washer Oil, Bolt, Flange Cover Protector Main Pump, Front Brake Seal Gasket Piston Spring Nut M6 Bolt Flange M8 x 22 Bolt, Flange M4x 11.5 Cover Plank Rubber Thin Film Cover Pump Front Brake Light Switch Bolt Flange M8 x 22 Right Hand Handle Bolt Flange 6 x 25 Oil Pump

Page 31 of 56

Figure F13 (Continued)
Figure No. F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13 F13

Ref. No. 42 43

Part No. XF250-A-0803-005 XF250-A-0803-006 XF250-A-0803-007 XF250-A-0803-008 XF250-A-0803-009 XF250-A-0803-010 XF250-A-0803-011 XF250-A-0803-012 XF250-A-0803-013 XF250-A-0803-014 XF250-A-0803-015 XF250-A-0803-016 XF250-A-0803-017 XF250-A-0803-018 XF250-A-0803-019 XF250-A-5-0805-001
Description Rubber Guide Pin Sleeve Front Brake Slide Pin Nut M8 Brake Shoe Rattle Plate Brake Piston Brake Piston Inner Seal Brake Piston Outer Seal Brake Pad Brake Pad Brake Pad Brake Pad Front Brake Slide Pin Rubber Guide Pin Sleeve Front Brake Caliper Bracket Brake Caliper Assembly Anti Lock Device

Page 32 of 56

Figure F14

FRONT FORK

Figure No. F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14 F14
Part No. XF250-A-0900-001 GB6187-86 XF250-A-0902-000 XF250-A-0901-000 XF250-A-0900-002 XF250-A-0900-007 XF250-A-0901-100 XF250-A-0900-005 XF250-A-0900-006 GB97.1-85 GB93-87 GB6170-86 GB91-86 XF250-A-0900-010 XF250-A-0900-011 XF250-A-0900-012 GB6170-86 GB6170-86 XF250-A-0903-000 XF250-A-0900-009 XF250-A-0900-008 XF250-A-0904-000 GB/T14212-93 DGB845-85 XF250-A-0900-004 XF250-A-0900-003 GB97.1-85
Description Shaft, Rear Swing Arm Self Locking Nut M14 x 1.5 Framework Oil Seal Rear Swing Arm Bushing Bushing Swing Arm Bolt Flange M8 x 25 Torque Link Washer 8 Spring Washer8 Hexangular Nut M8 Openings 2x20 Chain Adjuster Assembly Chain Adjuster Plate Chain Puller Hexangular Nut M6 Hexangular Nut M8 Case, Chain (Chain Guard) Cover Nut M5 Bolt Flange M6 x8 Chain Protest Pod I Chain 520-1 x 108l Self Tapping The Bolt Flange St4.8 x 16 Washer 8 Buffer, Chain Washer 10

Page 33 of 56

Figure F14-1
Figure No. F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1 F14-1
Part No. XF250-A-0900-001 GB6187-86 XF250-A-0902-000 XF250-A-9-0901-000 XF250-A-0900-002 XF250-A-0900-007 XF250-A-0901-100 XF250-A-0900-005 XF250-A-0900-006 GB97.1-85 GB93-87 GB6170-86 GB91-86 XF250-A-5-0900-010 XF250-A-5-900-011 XF250-A-9-0900-003 GB97.1-85 XF250-A-9-0903-000 XF250-A-0900-009 XF250-A-0900-008 XF250-A-0904-000 GB/T14212-93 DGB845-85 XF250-A-0900-004

Description Swing Arm Pivot Bolt Self Locking Nut M14 x 1.5 Framework Oil Seal Swing Arm Assembly Swing Arm Bushing Swing Arm Bushing Swing Arm Bolt Flange M8 x 25 Tip Rap Washer 8 Spring Washer T8 Nut M8 Openings 2 x 20 Chain Puller Chain Adjuster Plate Swing Arm Protector Washer 10 Chain Shell Cover Nut M5 Bolt Flange M6 x8 Chain Protest Pod I Chain 520-1 x 108l Self Tapping Bolt Flange ST4.8 x16 Washer

Page 34 of 56

Figure F15

REAR BRAKE

Figure No. F15 F15 F15 F15 F15 F15 F15 F15 F15 F15 F15 F15 F15 F15

Ref. No. 13 14

Part No. XF250-A-1003-000 GB6177-86 XF250-A-1003-001 XF250-A-1003-003 XF250-A-1003-002 XF250-A-1004-000 GB91-86 GB6170-86 GB96-85 XF250-A-1003-004 XF250-A-1003-005 XF250-A-1003-006 XF250-A-1003-007 XF250-A-1005-000
Description Plate Component, Rear Brake Nut M6 Back Brake Axle Arm Instruction Washer Bolt Back Brake Cover Openings 2x20 Nut M8 Washer 8 Rubber Washer Collar Bolt Brake Shaft Brake Spring Coil Rear Brake Shoe

Page 35 of 56

Figure F16

FUEL TANK

Figure No. F16 F16 F16 F16 F16 F16 F16 F16 F16 F16 F16 F16 F16 F16
Part No. XF250-A-1100-006 DD90E-1100-002 DD90E-1100-003 DD90E-1100-001 XF250-A-5-1101-000 DD90E-1100-005 GB5787-86 DD90E-1100-007 XF250-A-1100-005 XF250-A-1100-007 XF250-A-1102-000 XF250-A-1100-002 XF250-A-1100-003 XF250-A-5-1100-003
Description Bolt Flange M6 x 25 Fuel Tank Washer Washer and Grommet Fuel Tank Rubber Mat Fuel Tank Washer Spring Bolt Flange M8 x 35 Washer Washer and Grommet Rubber Mat, Fuel Tank Cock, Fuel Tank Fuel Line Clamp Fuel Line Fuel Line 4.5 x 8.5

Page 36 of 56

Figure F17

SEAT ASSEMBLY

Figure No. F17 F17 F17 F17 F17 F17 F17 F17 F17 F17 F17 F17 F17 F17

Ref. No. 11 12

Part No. XF250-A-1301-000 XF250-A-1311-000 GB5789-86 XF250-A-1303-000 XF250-A-1333-000 GB818-85 XF250-A-1300-008 DD90E-1100-001 DD90E-1100-003 GB96-85 XF250-A-2100-010 XF250-A-1300-001 XF250-A-1300-002 XF250-A-1302-000
Description Driver Seat Driver Seat Bolt Flange M8 x 14 Passenger Seat Passenger Seat M6 x 12 Ten Bolt Flange Plank Seat, Rubber Grommet Washer and Grommet Washer 6 Nut M6 Tape Saddle Seat Grab Strap

Page 37 of 56

Figure F18

BACKREST ASSEMBLY

Figure No. F18 F18 F18 F18 F18 F18 F18

Ref. No. 7

Part No. XF250-A-1307-000 XF250-A-1306-000 XF250-A-1304-000 XF250-A-1305-000 GB70-85 XF250-A-0200-003 XF250-A-0400-003

Description Plank Of Right Main Footstep Rubber Mat Of Right Main Footstep Pin Axle A8 x50 Screw M6 x 16 Module Of Right Main Footstep Nut M6 Forelock 2x20 Quakeproof Module Twist Spring Right Set Module Fame Of Right Main Footstep Screw M8 x 32 Plank Of Left Main Footstep Module Of Left Main Footstep Fame Of Left Main Footstep Rubber Mat Of Left Main Footstep Twist Spring Left Foot Brace Set Self Locking Nut 10 x 1.25 Bolt Flange 10 x 1.25 x 35

Page 41 of 56

Figure F19-1 (Continued)
Figure No. F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1 F19-1
Part No. XF250-A-1400-003 DD150E-3-1912-000 GB93-87 XF250-A-1908-002 XF250-A-1912-003 XF250-A-1912-001 GB96-85 XF250-A-1912-004 XF250-A-1912-002 XF250-A-1910-000 XF250-A-1913-000 DD150E-3-1908-000 XF250-A-1911-000 GB5787-86 GB97.1-85
Description Foot Brace Extension Spring Right Secondary Footstep Set Spring Coil Washer 10 Pin Axle Right Secondary Footstep Axle Step The Foot Rubber Set Washer 6 Rubber Mat Piece Washer 8 Card Module Right Card Module Left Left Secondary Footstep Set Left Secondary Footstep Axle M10 x 1.25 x 35 Washer 10

Page 42 of 56

Figure F20
Figure No. F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20 F20

Ref. No. 30 31

Part No. GB923-88 XF250-A-1402-000 XF250-A-1402-001 XF250-A-1402-003 GB91-86 XF250-A-1402-007 XF250-A-1402-005 GB5783-86 XF250-A-1405-000 XF250-A-1404-001 XF250-A-1405-008 XF250-A-1404-002 XF250-A-1405-100 XF250-A-1405-008 XF250-A-1405-003 XF250-A-1405-004 GB91-86 XF250-A-1405-006 XF250-A-1405-007 XF250-A-1405-001 XF250-A-0418-000 XF250-A-1406-003 XF250-A-1406-000 XF250-A-1406-100 XF250-A-1407-000 XF250-A-1406-004 GB6170-86 XF250-A-1406-006 XF250-A-1406-006 XF250-A-1406-007 XF250-A-1408-000 GB5783-86
Description Cover Nut M10 x 1.25 Rear Pedal Rear Brake Pedal Pivot Bolt Pin Openings 1.6 x 16 Pull The Pole Multiple Plank Bolt Flange M8 x 25 Rear Brake Rod Assembly Nut Pin Axle Brake Rod Spring Brake Rod Pin Rear Brake Pivot Cover Pin Axle Module Cotter Pin 2 x 20 Rubber Tube 15x19x60 Brake Return Spring Back Brake Lamp Switch Spring Back Brake Lamp Switch Line Variable Floor Pole Pin Axle Pedal Component, Gear Shaft Shift Pedal Rubber Shift Pedal Shift Rod End Cover Nut M6 Double Head Bolt Flange Double Head Bolt Flange Nut M6 Shifter Arm Bolt Flange M6 x 12

Page 43 of 56

Figure F21

COVERS

Figure No. F21 F21 F21 F21 F21 F21 F21 F21 F21
Part No. XF250-A-5-1602-001 XF250-A-5-1602-001 XF250-A-0202-004 XF250-A-0202-005 XF250-A-5-16001-001 XF250-A-5-1601-001 XF250-A-1602-003 XF250-A-1602-004 XF250-A-2400-078
Description Cover, Right (Battery Cover, Right) Right Protect Plank Rubber Washer I Rubber Washer II Cover, Left (Battery Cover, Left) Left Protect Plank Nut M6 Bolt Decal

Page 44 of 56

Figure F22

AIR CLEANER ASSEMBLY

Figure No. F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22 F22
Part No. XF250-A-1700-000 XF250-A-1700-007 XF250-A-1700-005 XF250-A-1700-006 XF250-A-1705-000 GB845-85 XF250-A-1704-004 XF250-A-1704-001 XF250-A-1704-003 XF250-A-1704-002 XF250-A-1700-014 XF250-A-1700-010 XF250-A-1701-000 XF250-A-1700-011 XF250-A-1700-009 XF250-A-1700-012 XF250-A-1700-013 GB5787-86 XF250-A-1700-008 XF250-A-1700-004 XF250-A-1700-002 XF250-A-1700-001 XF250-A-1700-003 XF250-A-0202-003
Description Air Filter Assembly (Air Cleaner) Rubber Plug Clamp Vent Hose Crankcase Vent Assembly Self Tapping Bolt, Flange ST5 x20 Tool Box Cover Tool Box Bolt Tool Box Bolt Fastener Tool Box Bolt Part Air Filter Hull Air Box Intake Manifold Air Filter Air Filter Metal Holder Hose Clamp Vent Hose Air Filter Outer Shell Cover Bolt Flange M6 x25 Air Box Drain Tube Is Into The Nut Carburetor Intake Boot Carburetor Intake Boot Clamp Rubber Grommet Washer M6 x12

Page 45 of 56

Figure F23

MUFFLER

Figure No. F23 F23 F23 F23 F23 F23 F23 F23 F23 F23
Part No. XF250-A-0301-013 XF250-A-1800-001 XF250-A-1803-000 XF250-A-1800-002 XF250-A-1800-004 GB6177-86 GB5789-86 GB5789-86 XF250-A-1800-005 XF250-A-1800-009
Description Exhaust Flange Nut M8 Exhaust Pipe Flange Exhaust Pipe Collars Exhaust Gasket 1801 A Rear Muffler Bracket Nut M8 Bolt Flange M8 x 22 Bolt, Flange M8 x 30 Exhaust Gasket Center Muffler Clamp

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Figure F23-1
Figure No. F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1 F23-1

Ref. No. 16

Part No. GB5789-86 XF250-A-1800-007 XF250-A-1800-006 GB6177-86 DD150E-3-1806-000 DD150E-3-1806-001 XF250A-1800-005 XF250-A-1800-009 XF250-A-8-1807-000 XF250-A-8-0803-003 XF250-A-0200-007 XF250-A-1812-000 XF250-A-9-1812-000 XF250-A-1811-000 GB5789-86 DD150E-3-1805-000 DD150E-3-1805-001
Description Bolt Flange M8x30 Rubber Washer and Grommet Rubber Grommet Nut M8 Right Muffler Bracket Right Muffler Bracket Rubber Washer and Grommet Center Muffler Clamp Right Muffler Bracket Bolt Bolt Flange, M8 x 20 Left Muffler Left Muffler Right Exhaust Gas Muffler Bolt Flange M8 x 22 Left Muffler Bracket Left Muffler Bracket

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Figure F24

REAR SHOCK ABSORBER

Figure No. F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24 F24
Part No. XF250-A-2000-000 XF250-A-2000-011 XF250-A-2000-010 XF250-A-2000-002 XF250-A-2000-004 XF250-A-2000-006 XF250-A-2000-007 XF250-A-2000-005 XF250-A-2000-003 XF250-A-2000-001 XF250-A-2001-000 XF250-A-2000-009 XF250-A-2000-008 GB5787-86 XF250-A-0200-006 XF250-A-0200-007 GB97.1-85
Description Rear Shock Absorber Lower Bushing Decal Damper Spring Top Bushing Upper Joint Rubber Bushing Nut Rubber Shock Damper Rear Shock Adjusting Ring Damper Unit Lower Shock Grommet Collar Bolt M10 x 1.25 x 38 Washer Bolt M8 x 16 Washer 8

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Figure F25

REAR FENDER

Figure No. F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25 F25

Ref. No. 21

Part No. XF250-A-2100-000 XF250-A-2100-001 XF250-A-210-000 XF250-A-2100-008 XF250-A-2100-014 XF250-A-2100-013 XF250-A-2100-011 GB848-85 DD90E-2100-022 GB97.1-85 XF250-A-2500-004 XF250-A-0301-013 XF250-A-2100-003 XF250-A-0309-008 XF250-A-2101-000 GB96-85 XF250-A-2100-007 XF250-A-2100-017 XF250-A-2100-015 DD90E-1700-005 XF250-A-2100-010
Description Rear Fender Assembly Stop Rear Fender Bracket, Right Washer Special Bolt Flange Bolt Flange M10 x 40 Washer 10 Bolt, Flange M8 x 32 Washer 8 Bolt, Flange M 8 x16 Cover Nut M8 Washer Rubber Taillight Rubber Grommet Rubber Washer and Grommet Washer 6 Bracket, Left Self Locking Nut M8 Washer Washer Ring Nut M6

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Figure F26

TOOL KIT

Figure No. F26 F26 F26 F26 F26 F26 F26 F26 F26 F26
Part No. XF250-A-2200-000 XF250-A-2200-010 XF250-A-2200-008 XF250-A-2200-004 XF250-A-2200-006 XF250-A-2200-001 XF250-A-2200-005 XF250-A-5-2200-001 XF250-A-5-2200-002 XF250-A-5-2200-003
Description Tools Tool Bag Wrench Box Shaft Screw Driver Wrench Hex Spanner 8-10 Grip Spanner 12-13 Pliers Spanner Pin

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Figure F27

FRONT FENDER

Figure No. F27 F27 F27 F27 F27 F27 F27 F27
Part No. XF250-A-8-2700-000 GB5787-86 XF250-A-8-2701-000 XF250-A-2700-001 XF250-A-8-2701-003 GB5783-86 XF250-A-0301-004 DD90E-270-003
Description Front Fender Set Bolt, Flange M6 x 16 Plate, Front License License Plank Bracket, Front Fender Screw M6 x 12 Nut M6 Clamp

Page 51 of 56

Figure F28

SIDE BOX

Figure No. F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28 F28
Part No. XF250-A-4000-000 XF250-A-4000-000 XF250-A-4000-001 XF250-A-4005-000 XF250-A-4002-000 XF250-A-4004-000 XF250-A-4000-005 XF250-A-4001-000 XF250-A XF250-A-4000-003 XF250-A-4003-000 XF250-A-4000-009 XF250-A-2840-009 GB96-85 DD90E-1100-002 GB5781-86 XF250-AJ-2840-012

Description Side Box Assembly Bolt Self ST2.9 x 12 Washer Side Box, Cable Side Box, Body Lid Pin Lid Rubber Gasket Side Box, Cover Bolt Self ST2.9 x 14 Paper Reflector Lock Rubber Mat Washer 6 Washer Screw M6 x 30 Rubber Washer

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Figure F29

REAR BOX AND LIGHTS

Figure No. F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29 F29
Part No. GB845-85 XF250-A-0308-001 XF250-A-0305-005 XF250-A-0302-003 XF250-A-0308-100 XF250-A-0308-002 XF250-A-0302-006 XF250-A-0306-000 XF250-A-0308-003 XF250-A-0308-004 XF250-AJ-0305-021 XF250-A-0301-013 XF250-AJ-2-1300-000 XF250-A-0612-000 XF250-AJ-2-2810-10 GB5787-86 GB70-85 XF250-AJ-2-2810-101 XF250-AJ-2810-005 XF250-A-0305-000 XF250-A-0308-000
Description Self Tapping Bolt Flange ST42 x 24 F-H Back Left Lamp Shade Bulb, Blinker Front 12v10/3w Cushion Rubber Back Left Lamp Shell Phillips Bolt Rubber Seat Of Back Steering Lamp Gum Set Fixed Position Set Set Cover Nut M8 Side Box Self Locking Nut M8 Set I Screw M8 x 65 Screw M8 x 50 Set II Baggage Box Stop Back Left Steering Lamp Set Back Right Steering Lamp Set

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Figure F30

WINDSHIELD

Figure No. F30 F30 F30 F30 F30 F30 F30 F30 F30 F30 F30 F30
Part No. XF250-A-0700 GB96-85 DD90E-2100-012 DD90E-2100-008 XF250-AJ-2310-001 DD90E-2100-022 XF250-AJ-2312-000 XF250-AJ-2310-007 GB6187-86 XF250-A-0200-006 GB6170-86 XF250-AJ-2310-008
Description Screw M8 x 25 Washer 8 Windshield Collar Windshield Grommet Windshield Screw MWindshield Clamp Windshield Bracket Arm Nut Self M8 Washer Nut M8 Windshield Bracket Arm

Page 54 of 56

Figure F31

TAILLIGHT AND REAR RACK

Figure No. F31 F31 F31 F31 F31 F31 F31 F31
Part No. XF2520-AJ-2-0306-000 GB845-85 DD90E-0306-002 DD90E-0306-001 DD90E-0306-100 DD90E-0306-200 GB818-85 XF250-AJ-2-1308-000
Description Tailgate Assembly Screw Tapping St3.9 x 30 Taillight Shell Bulb, Taillight Socket Taillight Light Hull Module Screw M6 x 12 Rear Luggage Rack

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Figure F32

REAR BOX

Figure No. F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32 F32
Part No. XF250-AJ-2-3000-000 XF250-AJ-2-3001-000 GB819-85 GB6172-86 XF250-AJ-2-300-011 GB818-85 XF250-AJ-2-3002-000 XF250-AJ-2-3000-007 XF250-AJ-2-3000-005 XF250-AJ-2-3000-001 XF250-AJ-2-3000-002 XF250-AJ-2-3000-010 XF250-AJ-2-3000-012 XF250-AJ-2-3003-000 XF250-AJ-2-3000-017 GB65-85 GB95-85 XF250-AJ-2-3000-018 GB5781-86 XF250-AJ-2-3000-009 GB6170-86 XF250-AJ-2-3000-015 XF250-AJ-2-3000-014 GB847-85 XF250-AJ-2-3000-004 XF250-AJ-2-3000-019 GB802-88 XF250-AJ-2-3000-008 XF250-AJ-2-3000-006 XF250-AJ-2-3001-002 XF250-AJ-2-3000-016 XF250-AJ-2-3000-013 XF250-AJ-2-3000-003

Description Tail Box Assembly Tail Box Top Bolt M5 x 8 Nut M5 The Tail Box Matches The Leaf Bolt M5 x 13 Tail Box Inside Reflector Press The Plank Reflector Decorate 1 Decorate 2 Tail Box Antenna Tail Box The Inside Decorate Along Tail Box Private Parts Tail Box Mat Set Bolt M4 x12 Washer 4 Tail Box Mat Scale board Bolt M8 x40 Installs To Press The Plank Nut M8 Three Locks Is Total Decorate The Piece Screw Tapping St 2.9 x 8 Tail Box Empress Reflector Tail Box Rubber Mat Nut M6 Reflector Press The Plank R Reflector Tail Box Upper Cover Rubber Mat Glint Paper Paper Sticker

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PIONEER MOTORS USA

MODEL XF250-A

PARTS CATALOG
Note: Please be advised that some Figure and References contained in this parts catalog may not apply to the model of Pioneer XF250-A motorcycle that is sold in the United States. Not all features or parts are available for the model sold in the United States.
The online manual is provided for reference. Without prior permission from Pioneer Motors USA, L.L.C., no part of this manual may be reproduced, duplicated or quoted in any form at any time. The data, explanations, photographs and specifications contained in this manual depict current products at time of publication. Due to improvements and a variety of other reasons, there may be some difference between the manual and your product. Pioneer Motors USA, L.L.C. reserves the right to revise or modify the specifications, parts information or any other information contained in this manual at any time without prior notice.