LWT-32000/32000S: 32" Widescreen LCD HDTV For The Lodging Industry
32" Widescreen LCD HDTV with integrated hospitality Video on Demand (VOD) support offers a high definition picture and user-friendly functions. Other features include: Commercial-ready with integrated Technicians Menu 1366 x 768 resolution 400 cd/m2 brightness 1200:1 contrast ratio Dual HDMI connectivity Detachable TV stand for easy wall-mounting

Features

High definition hybrid NTSC and ATSC tuner 480i/480p/720p/1080i digital compatibility 16:9 aspect ratio 3D comb filter Built-in speakers system with SAP stereo sound (10 watts x 2) Pre-programmed sound controls User-selectable tone controls Trilingual on-screen display (English, Spanish, French) Sleep timer Closed captioning Integrated hospitality Video On Demand (VOD) support Commercial-ready with integrated Technicians Menu Pro-Idiom support (Available on "S" models only) Detachable TV stand for easy wallmounting

Specifications

Resolution: 1366 x 768 Brightness: 400 cd/m2 Contrast ratio: 1200:1 Response time: 8 ms Viewing angle: 176 x 176
Products, features, specifications and appearances are subject to change without notice.

Inputs/Outputs:

2 HDMI inputs VGA (D-Sub) input 2 component video inputs (YPbPr) S-video input 2 composite video inputs Coaxial RF input 5 audio inputs Audio output Headphone jack
Toll Free Order Line 1-800-742-6758

18:40 20:00

Mondav Mav 24th
08:30 Ebner Auditorium - Registration and Life Sciences Posters Displaying.

PLENARY SESSION II

Chairperson: A. Amsterdam
S. Cohen, Weizmann Institute of Science, Rehovot. SCANNED PROBE MICROSCOPIES - NOVEL EXPERIMENTS WITH A DEVELOPING TECHNIQUE. A. Yonath, T. Arad, J. Piefke, Z. Berkovitch-Yellin, M. Eisenstein. F. Franceschi, I. Agmon, M. Laschever, S. Weinstein.Weizmann Institute of Science, Rehovot, Max-Planck-Institute Berlin, Hamburg, Germany. ELECTRON MICROSCOPY OF CRYSTALLINE RIBOSOMAL PARTICLES. M. Neeman, Weizmann Institute of Science, Rehovot. SELF DIFFUSION MAPPING BY NMR MICROSCOPY: APPLICATION TO BIOMEDICAL RESEARCH. H. Gundlach, Carl Zeiss, Germany. PROGRESS IN LIGHT MICROSCOPY BY MEANS OF MULTICOLOR FLUORESCENCE MICROSCOPY, DIGITAL IMAGING AND CONFOCAL LASER SCANNING MICROSCOPY.
-6Parallel Sessions: Life Sciences - Ebner Auditorium; Material Sciences - Schmidt Auditorium. LIFE SCIENCES IV
Chairpersons: E. livne and R. Coleman
C.J.F. Van Noorden, Academic Medical Center, University of Amsterdam, The Netherlands. QUANTITATIVE ENZYME HISTOCHEMISTRY AS A TOOL FOR THE IN SITU ANALYSIS OF PATHOLOGICAL CHANGES IN CELLULAR METABOLISM. H. Arad-Dann, U. Beller, R. Haimovitch. Y. Gavrieli, S.A. Ben-Sasson, Shaare-Zedek Medical Center, Jerusalem. Hebrew UniversityHadassah Medical School, Jerusalem. IMMUNOHISTOCHEMISTRY OF PHOSPHOTYROSINE RESIDUES: INDENTIFICATION OF DISTINCT INTRACELLULAR PATTERNS IN EPITHELIAL STEROIDOGENIC TISSUES. D. Dayan, M. Wolman. School of Dental Medicine. Sackler Faculty of Medicine. Tel-Aviv University. USE OF PICROSIRIUS STAINING AND POLARIZATION MICROSCOPY IN DIFFERENTIATING COLLAGENS IN TISSUE. A. M. Nahir, D. Shomrat. M. Awad. Rambam Medical Center, Haifa, Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa. CHONDROITINASE ABC AFFECTS INTRA-CELLULAR CHONDROCYTIC ENZYME ACTIVITY. D. Hanein, H. Sabanay, L. Addadi. B. Geiger. Weizmann Institute of Science. Rehovot. SELECTIVE INTERACTIONS OF CELLS WITH CRYSTAL SURFACES. Chairperson: A. Venkert
MATERIAL SCIENCES IV 12:00
E. Manor, H. Ni, C.G. Levi, R. Mehrabian. Ben-Gurion University of the Negev, Beer-Sheva. University of California, Santa-Barbara, Carnegie Mellon University. Pittsburg. CERAMIC MATRIX COMPOSITES PRODUCED BY OXIDATION OF LIQUID Al ALLOY. I. Grlmberg, B. Z. Weiss, Technion - Israel Institute of Technology, Haifa. THERMAL STABILITY OF EPITAXIAL GROWTH IN COEVAPORATED NlSi2o.2 T H 1 N ALLOY FILMS. T. Ben David, Y. Lereah. G. Deutscher, R. Kofnian, P. Cheyssac. Tel-Aviv University. SOLID LIQUID TRANSITION IN ULTRA FINE LEAD PARTICLES (R=20-500A). E. Napchan, Imperial College, London. England. MONTE-CARLO SIMULATIONS OF CATHODOLUMINESCENCE EFFECTS IN SEM. LUNCH BREAK POSTER SESSION

A. Nyska, A. Zuckerman, Y. Weisman, M. Malkinson, A. Lublin, Klmron Veterinary Institute, Beit Dagan, School of Veterinary Medicine, Hebrew University, J e r u s a l e m , Israel Institute of Biological Research, Ness-Ziona. USE OF IMAGE ANALYSIS FOR THE STANDARDIZATION OF INFECTIOUS BURSAL DISEASE VIRUS VACCINES. R. Coleman, M. Nahir, Z. Tanne, D. Shomrat, M.B.H. Youdim, Bruce Rappaport Faculty of Medicine.Technlon - Israel Institute of Technology, Haifa.
SUCCINIC DEHYDROGENASE ACTIVITY IN CARDIAC MYOCYTES AND CORRELATED ULTRASTRUCTURE IN IRON-DEFICIENT RATS.
R. Coleman, Z. Tanne, M.B.H. Youdim, Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa. THE ADRENAL CORTEX OF IRON-DEFICIENT RATS: AN ULTRASTRUCTURAL STUDY. H. Levanony, G. Galili, Y. Altschuler, Weizmann Institute of Science, Rehovot. THE INTERNALIZATION PROCESS OF WHEAT STORAGE PROTEINS AND THE BIOGENESIS OF PROTEIN BODIES. V. Holdengreber, T. Hayimi, Y. Ben-Shaul, Tel-Aviv University. IMMUNOHISTOCHEMICAL ANALYSIS OF MOLECULES INVOLVED IN MORPHOGENESIS OF EMBRYONIC RETINA D. Levanon, H. Stein, Bruce Rappaport Faculty of Medicine, Technion- Israel Institute of Technology, Haifa. Rambam Medical Center, Haifa, TANNIC ACID ENHANCES THE FORMATION OF COMPLEX(ES) MADE OUT OF COLLAGEN AND CHONDROITIN SULFATE.

- 10 Mondav Mav 24th

POSTER SESSION II
E. Shezen, A. Lossos, O. Abramsky, T. Siegal. Hadassah-Hebrew University Hospital, Jerusalem.
EXPRESSION OF a-SMOOTH MUSCLE ACTIN IN GLIOTIC SCAR, PRIMARY AND METASTATIC BRAIN TUMORS.
E. Shezen, E. Okon, H. Ben Hur, O. Abramsky. Hadassah-Hebrew University Hospital, Jerusalem, Kaplan Hospital, Rehovot. IMMUNOHISTOCHEMICAL MAPPING OF CYTOKERATIN DISTRIBUTION IN HUMAN THYMUS. A. M. Nahir, D. Shomrat, M. Awad, Rambam Medical Center, Haifa. Bruce Rappaporl Faculty of Medicine, Technion - Israel Institute of Technology, Haifa. DECREASED ENZYME ACTIVITY WITH AGE IN RABBIT CHONDROCYTES. M. Weinreb, D. Halperin, R. Baron, School of Dental Medicine, Tel-Aviv University, Yale University, New-Haven, CT. RAT OSTEOCLAST PRECURSORS EXPRESS A VITRONECTIN RECEPTOR AND A CHLORIDE-BICARBONATE EXCHANGER. D. Dayan, H. Tal, T. Waner. A. Nyska, School of Dental Medicine, Tel-Aviv University, Life Science Research, Ness Ziona, Kimron Veterinary Institute, Beit Dagan. OXODIPINE-INDUCED GINGIVAL HYPERPLASIA IN BEAGLE DOGS: POLARIZING MICROSCOPY OF PICROSIRIUS RED-STAINED COLLAGEN. D. Lewinson, P. Shenzer. Z. Hochberg, Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology. Haifa.

MOLECULAR AND CELLULAR RESPONSE OF GLIAL CELLS TO AXONAL INJURY IN FISH AND RODENT CENTRAL NERVOUS SYSTEMS
Michal Schwartz1, Vered Lavie1, Arieh Solomon2, Shoshana Eitan1, Snejana Ben-Bassat1, liana Cohen' and Eran Blaugrund1. 'Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel, and 2Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel Aviv University, Sheba Medical Center, Tel Hashomer, Israel. The poor ability of neurons of the mammalian central nervous system (CNS) to regenerate their injured axons, unlike their counterpart in lower vertebrates, has been attributed to the degree of permissiveness of the injured nerves to axonal growth. Such permissiveness is thought to be the net outcome of several cellular and molecular factors, among them the effects of astrocytes (the potentially scar-forming cells) and of oligodendrocytes (the growth-inhibitory cells), and the presence or absence of soluble factors that inhibit or support growth. In an attempt to evaluate the specific contributions of the above factors and to determine how their response to injury is controlled so as to make them conducive to axonal growth, we compared the response to injury in adult fish and rodent optic nerves, focusing on their nonneuronal elements. In the course of our studies of the spontaneously regenerating CNS of the fish, we observed that axonal regeneration in the fish optic nerve is accompanied by the production of a factor which is selectively cytotoxic to oligodendrocytes of both fish and mammals. This factor was recently identified as a dimeric form of interleukin 2 (IL-2), possibly resulting from the posttranslational modification of monomeric IL2 mediated by the enzyme transglutaminase (Eitan et ah, 1992; Eitan and Schwartz, 1993). In fish, the presence of the cytotoxic factor has been correlated with the postinjury inflammatory reaction and a low postinjury number of oligodendrocytes (Sivron et al., 1991). In addition, astrocytes of injured optic nerves in the fish behave differently from those in mammals, with respect to their postinjury migratory ability. Thus, while in fish the astrocytes of injured optic nerves migrate out of their zone of proliferation, in rodents they remain within their zone of proliferation and a glial scar is subsequently observed. Migration of astrocytes across the site of injury seems to be a necessary condition in order to support the regrowth of axons. In view of the above, we treated the injured mammalian optic nerve immediately after injury by a local application of soluble factors derived from regenerating fish optic nerves. In the treated animals, axons were seen to grow across the injury site, penetrating deep into the distal stump. In line with the role of astrocytes suggested above, we also observed that the newly growing axons were embedded in astrocytes that had apparently exploited their potential ability to migrate. We therefore conclude that the rodent optic nerve is amenable to treatment aimed at inducing axonal regeneration.

**Supported by research grants GIF 1-170 in cooperation with Dr. D. Godde, Ruhr University Bochum, SFB-184 in cooperation with R.Herrmann and W.Rudiger, LudwigMaximilian University, Munchen, Germany and BARD-IS-1986-91R in cooperation with H. Pakrasi, Washington University St. Louis U.S.A. 1) Prasil.O., Function and pp.295-348 Adir. N. and Ohad I. (1992) in "The Photosystems, Structure, Molecular Biology" J.Barber ed. Elsevier Science Publishers B.V.

- 27 -

INTRACELLULAR TRANSPORT OF WHEAT SEED STORAGE PROTEINS: SIGNALS AND SUGGESTED MECHANISMS
G. Galili, H. Levanony, Y. Altschuler, N. Rosenberg, S. Giorini, Y. Shimoni, N. Shani, and H. Karchi. Department of Plant Genetics, The Weizmann Institute of Science, Rehovot. Ultra structural and molecular analyses were performed on developing wheat endosperm to study the intracellular transport of the storage proteins and the subcellular site of their packaging and accumulation in protein bodies (PB). At early to mid stages of grain maturation, the majority of the endosperm cells contained central vacuoles where most of the PB accumulated as inclusions. Golgi apparati, containing storage proteins, were also detected but these were rare, suggesting that their participation in the transport of the storage proteins to vacuoles is limited. Many of the endosperm cells exhibited a considerable amount of PB that were surrounded by endoplasmic reticulum (ER) membranes. Small, electron lucent vesicles were often detected around the surfaces of these PB, or attached to them, suggesting that such attachments and subsequent fusion of the vesicles could lead to the formation of small vacuoles containing PB inclusions. This is a novel intracellular route which is analogous to autophagy. Immunogold labeling with serum raised against BiP, an ERlocalized molecular chaperone, demonstrated that this protein was present within the PB that were localized either within the ER or inside vacuoles. This confirmed that some of the PB were formed within the ER and that the Golgi was apparently not involved in their transport to vacuoles. In order to identify signals which control the sorting of wheat storage proteins within the ER, either for retention inside this organelle or for export to the Golgi, we have expressed wild type and modified proteins in the heterologous systems of Xenopus oocytes and yeast. We found that retention of the storage proteins within the ER was determined by recognition of a specific signal located on their N-terminal regions by specific, evolutionary conserved ER factor/s. Export of the storage proteins was related to correct folding by disulfide bond formation of the C-terminal region of the storage proteins. We also found that the retention and export signals were competitive. Attempts to identify the ER factor/s that control the transport of the wheat storage proteins inside the organelle are in progress.

Laboratory of Cell Biology and Histology, Academic Medical Centre, University of Amsterdam, The Netherlands
Certain changes in the metabolism of tissues or cells can be used as sensitive parameters for the demonstration of pathological or toxicological effects. Ideally, one would like to study these effects in an non-invasive way in living cells or tissues. However, despite the fact that cytochemistry of living cells with the use of fluorescent probes is a rapidly expanding new field in cell physiological research its applications are still rather limited. A good alternative is the use of unfixed cryostat sections or cell preparations for metabolic research. When frozen properly, unfixed biological material has such good morphology that it can be used for ultrastructural investigations. A fair number of valid and reliable histochemical methods have been developed to analyse metabolic processes in unfixed preparations, such as the activity of regulatory enzymes of relevant metabolic pathways, but also proteolytic activity and production of oxygen radicals and their metabolites. The validity of histochemical methods allows quantification and scanning and integrating cytophotometry has been used for decades for this purpose. However, image analysis, flow cytometry and confocal scanning laser microscopy have been introduced in the field to analyse amounts of final fluorescent or coloured reaction products. Until recently, quantitative enzyme histochemistry was performed to measure amounts of final reaction product precipitated after incubation for a set period of time (end point measurements). Furthermore, the capacity of an enzyme rather than its activity was detected because saturating substrate concentrations were used that yielded zero order kinetics. The latest approach is to study on the one hand the kinetics of enzyme reactions by varying substrate concentrations in the incubation media and on the other hand by analysing the reactions in time while they proceed. For the latter purpose, incubations are performed on the stage of a microscope attached to the measuring equipment. Finally, physiological substrate concentrations can be detected in situ in unfixed sections or cell preparations and these parameters taken together enable us to analyse in situ fluxes of substrates through metabolic pathways which should be a good reflection of the metabolic status of tissues and cells in vivo.
Literature C./.F. Van Noorden and W.M. Frederiki (1992): Enzyme Histochemistry. A Laboratory Manual of Current Methods. Oxford University Press Inc., New York.

irradiated

regenerating
N. Weiss, A. Bibikova and U. Oron Dept. of Zoology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, 69978 Israel
The expression of desmin was investigated in normal and He-Ne irradiated regenerating rat and toad gastrocnemius muscles using immunocytochemical
methods. In the first few days post-injury presumptive myoblasts were positively stained with desmin while other monocleated cells remained
unstained. At a later stage the cytoplasm of the myotubes was intensely stained with antidesmin. The cytoplasm of the newly-formed young ayofibers in the injured area also reacted positively to desmin with decrease in the intensity of the staining with the maturation of these structures. At all time intervals there were more mature myogenic structures in the injured zones of the laser irradiated muscles as compared to non-irradiated muscles, it is concluded that desmin can serve as a good marker for myogenic cells and structures during regeneration of skeletal muscles. The process of skeletal muscle regeneration following injury is markedly promoted by low energy direct laser irradiation during the regeneration process.
IN SITU ANALYSIS OF PHYSIOLOGICAL AND PATHOLOGICAL ANCIOGENESIS Eh Keshet, Eran Bacharach, Dorit Shweikj and Ahuva Itin. Depi of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem. Angiogenesis - denned as the formation of new blood vessels as extensions of preexisting vessels - is a fundumental developmental process, but a mostly quiescent process in the adult, healthy organism. The angiogenic potential, however, is maintained in a constant state of readiness, capable of responding with a rapid capillary growth towards a positive stimulus eminating from under-perfused tissues. A number of factors have been shown capable of eliciting capillary growth in a model systems Yet very little is known about angiogenic factors operating in the context of natural neovascularization. We have, therefore, devised a new strategy to assess the //; vivo relevance of putative mediators of angiogenesis, based on retrieval of specimens that represent sequential stages in natural neovascularization, and elucidating spatio-temporal patterns of expression of candidate genes throughout the ongoing process. This procedure also provides a molecular access to downstream responses to the angiogenic stimulus. Here we report the following findings : A. Hypoxia, resulting from insufficient perfusion, is thought to act as a natural trigger of a feedback neovascularization response Yet, the nature of the presumptive hypoxia-induced angiogenic factors that mediate this response has not been elucidated. We show that vascular endothelial growth factor (VEGF) is an hypoxia-inducible angiogenic factor. Moreover, we show that VECF is specifically elaborated by ischemic cells in different settings of ischemiainitiated, pahthological neovascularization. ft. We show that plasminogen activator inhibitor type I (PAID is activated during angiogenesis, specifically in cell juxtapositioned next to cells producing the respective protease activator. We propose that this apposition-dependent regulation is required to attain the correct proteolytic balance, allowing endothelial cell invasiveness, on one hand, and preventing excessive damage to the neovascularized tissue, on the other.

WELD CHARACTERIZATION OF NEW METASTABLE BETA-21S TITANIUM ALLOY P. Benaim#*. D. EHezei**, E. AbranW*. A. Stern#* and A. Venker^ # Nuclear Research Center-Negev P.O.Box 9001 Beer-Sheva * Ben-gurion University, P.O.Box 653 Beer-Sheva. Metastable beta titanium alloys have excellent formabilhy. However, because of their high oxidation rate and poor creep resistance they can be used as structural materials only at limited temperatures. The new metastable titanium alloy, beta-2IS with the composition of Ti-15%Mo-2.7Nb-3%Al-0.2%Si, was developed to overcome these limitations of the beta alloys. This alloy is a promising candidate for future applications, especially for the aerospace industry. Good weldability will increase the alloy utility for engineering applications. The primary purpose of this work was to develop an understanding of the micro structural evolution during welding with gas tungsten arc (GTAW) and with electron beam (EBW), as a function of the heat input and therefore the cooling rate. Metallurgical characterization of the welded specimens was carried out using optical and electron microscopy. The determination of the phases and the chemical compositions of the welded zones and the interfaces between the weld and the metal, was carried out using x-ray diffraction and electron probe microanalysis techniques. The microprobe results showed composition variations in the fused zone and the heat affected zone. The composition variations were affected both by the heat input and by the welding processes.
TEM observation of giant nested fullerenes in Mo(W)Sz L. Margulis, G. Salitra and R. Tenne Dept of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel
Nested fullerenes (the so-called onion or Russian-doll shape) is one of the subgroups of polyhedral structures. A related structure to these polyhedra is the nested nanotubules, in which the apex consists of a half fullerene molecule. Previously, closed structures were believed to exist only in carbonaceous materials, but quite recently we discovered them also in the layered semiconductor WS2. One would expect that similar polyhedral structures could exist in other layered materials. Here we report on nested fullerene-like structures in both tungsten and molybdenum disulfides prepared by heating thin W or Mo films on quartz in a H2S atmosphere. It was found advantageous, though not absolutely necessary, for the fullerenes' growth to first oxidize the sample at 500C in open air for half an hour. The oxide film was subsequently fired at 850-1050C for various periods of time in a stream of H2S and N2/H2 mixture. The giant fullerenes grow from an amorphous phase. In addition to the giant fullerenes, nested tubulenes were also observed. The fullerenes form in the bulk of the matrix, while the cylinders grow into voids, due probably to the asymmetry in the local environment surrounding the crystals. The closed nature of the nested fullerenes and tubulenes has been verified by electron diffraction and lattice imaging. The geometrical model was proposed which allows to explain an appearance of different angles at the apex of the giant fullerenes.

along single myofilamentB, in vacuoles of different size and were seen
budding through the cellular membrane.

USE OF

ANALYSIS

STANDARDIZATION

INFECTIOUS
BURSAL DISEASE VIRUS VACCINES. A.NYSKA(1),A. ZUCKERMAN(2), Y. MEISMAN(l), M. MALKINSON(l), A. LUBLIN(l). l.KIMRON VETERINARY INSTITOE, BEIT DAGAN, AND THE SCHOOL OF VETERINARY MEDICINE, THE HEBREW UNIVERSITY JERUSALEM. 2. ISRAEL INSTITUTE OF BIOLOGICAL RESEARCH (IMAGE ANALYSIS), NESS-ZIONA. Guraboro Disease(GD) or Infectious Bursal Disease(IBD) is a viral disease of chickens that depresses the bird's immune system. The primary target organ for the IBD virus is the bursa of Fabricius., which plays a key role in B-cell immunity. Histological examination of pathological changes in the bursa is a critical factor in determining the extent of protection afforded by both live and inactivated IBD vaccines. We have adapted the image analysis technique to evaluate damage to bursal follicles objectively and by this, to assess the efficacy of IBD vaccines. The experiment included 3 groups of 10 "Anak" chicks: a nonvaccinated, noninfected group(NVNI); an nonvaccinated, IBD virus-infected group(NVI); and a vaccinated, IBD virus infected group(VI). Vaccination was carried out at 14 days oi age with 0.5 ml of inactivated vaccine (B.L.T., Jerusalem). Intra-ocular challenge with a virulent field virus was carried out 15 days post-vaccination. Ten days later, the chicks were sacrificed, their bursae were weighed, formalin fixed and 5 micron sections cut for histological examination and stained by Hematoxylin and Eosin(HE). Morphometric analysis was performed on histological sections, using the CUE-3 image analysis system (GALAI, Migdal Haemek, Israel). Analysis of the bursa included the total area and the integrated optical density(IOD) of 10 adjacent follicles. The ratio of IOD to area was calculated from both parameters. Correlation of the morphometric values to bursa-spleen weight ratio was also examined. The results of the morphometric measurements were: Area(microns) IOD(IOD units) control(NVNI) 6.25x 4 392x 2 VI NVI 5.54x 4 2.74x 1 0

254x 2 117x 2

All the differences between the groups were statistically significant (p<0.001). The results of the morphometric measurements were in agreement with alterations in the organ weights. The use of image analysis technique in the present case enabled us to perform an accurate and rapid assessment of the bursal damage, and by this, to evaluate the efficacy of the immunity afforded by the vaccine.

SUCCINIC DEHYDROGENASE ACTIVITY IN CARDIAC HYOCYTES AND CORRELATED ULTRASTRUCTURE IN IRON-DEFICIENT RATS R. Coleman1, H. Nahir2, Z. Tanne3, D. Shomrat^ & M.B.H. Youdim3
^Division of Morphological Sciences, 2H.Schussheim Rheumatology Unit, Department of Pharmacology, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel
Male Sprague-Dawley rats aged 3 weeks that were maintained on an iron-deficient diet for 4-5 weeks developed severe anemia, which was accompanied by marked cardiac hypertrophy (cardiomegaly). The major morphological and cytochemical changes were seen in the left ventricle. Succinic dehydrogenase activity was localized cytochemically and the colored formazan reaction product was quantified using scanning and integrating microdensitometry with a Vickers M86 microdensitometer. The myocytes of the left ventricle of the iron-deficient rats showed a marked reduction in SDH activity. This correlated well with the ultrastructure of the myocytes as seen in transmission electron microscopy. In the iron-deficient rat hearts the myocytes showed disruption of sarcomeres, myofilament loss, myofilament discontinuities and marked interfibrillar edema. The interfibrillar mitochondria were enlarged and showed degenerative changes in the matrix including the formation of electrondense bodies. When the severely iron-deficient rats were restored to a normal diet for two weeks, hemoglobin levels recovered, though the heart remained hypertrophied and the myocytes continued to show severe degenerative changes. Supported in part by the Fund for Promotion of Research (Technion 184-197)
THE ADRENAL CORTEX OF IRON-DEFICIENT RATS: AN ULTRASTRUCTURAL STUDY Raymond Coleman*, Zahava Tanne** & Moussa B.H. Youdim** ^Division of Morphological Sciences, **Department of Pharmacology, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 3 week old male Sprague-Dawley rats were placed on an iron-deficient diet for 4-5 weeks. These rats developed severe anemia with markedly reduced hemoglobin levels (4.100.20Hb g% compared with controls 12.74+O.15Hb g%). On sacrifice the adrenal glands were removed and processed for light and transmission electron microscopy. The major histological and ultrastructural changes in response to the iron-deficiency were seen in the adrenal cortex in the zona fasciculata, especially in cells of the outer part of this layer, and to a lesser degree in cells of the zona reticularis. In these regions the cells developed severe degenerative stuctural changes, especially in the mitochondria. The mitochondria were frequently grossly enlarged and

RAT OSTEOCLAST PRECURSORS EXPRESS A VITRONECTIN RECEPTOR AND A CHLORIDE-BICARBONATE EXCHANGER. M. Weinreb 1, D. Halperin 1 and R. Baron 2. 1- Tel-Aviv University School of Dental Medicine, ISRAEL 2- Yale University school of Medicine, New-Haven, CT, USA Osteoclasts are the cells responsible for bone resorption. They are large, multinucleated, hematopoietic in origin and express distinct differentiation markers such as tartrate-resistant acid phosphatase (TRAP) and calcitonin receptor (CTR). Their precursors are mononucleated and have b^en shown to express TRAP and CTR as well. In recent years, mature osteoclasts have been shown to express other markers, namely\a vitronectin receptor (VNR) and a chloride-bicarbonate exchanger (CBE) which play an important role in matrix attachment and pH regulation, respectively. In order to expand our knowledge on the characteristics of osteoclast precursors, this study used immunohistochemistry to examine whether these cells also express VNR and CBE. Frozen metatarsal sections of 1-4 day-old Sprague-Dawley rats were fixed with 1% paraformaldehyde/PBS and reacted histochemically for TRAP using naphtol-ASTR phosphate. Mononuclear, 'On-oositive cells were found along the perichondrium and perios.~\.m, i,s expected, /djacent sections were reacted with polyclonal antibc-ies against a subunit of the VNR or synthetic sequences of the CBE, using either a peroxidase-labeled secondary antibody or an avidinbiotin-peroxidase method with AEC as substrate. Both mature, i?ultinucleated osteoclasts (in the forcing bone marrow) and mononuclear cells in the perichondrium and periosteum were positive for both markers. These findings indicate that osteoclast precursors express the VNR and CBE before their invasion of the calcified rudiment and subsequent fusion to multinucleated cells. This research was supported by the basic research foundation administered by the Israel Academy of Sciences and Humanities.
Oxodipine-induced Gingival Hyperplasia in Beagle Dogs: Polarizing Microscopy of Picrosirius Red-stained Collagen. D. Dayan,^ H. Tal,* T. Waner,^ A. Nyska.* ^Section of Oral Pathology and Oral Medicine, a Section of Periodontology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, 3 Life Science Research, Ness Ziona, ''Kimron Veterinary Institute, Beit Dagan, Israel. Oxodipine, a new light stable dihydropyridine-type calcium antagonist, was administered orally twice daily for a period of 90 days to 8 beagle dogs (4 males, 4 females) in a dose of 24 mg/kg weight/day. At the end of treatment, the dogs were necropsied and blocks were prepared from gingiva of the following sites: the mandible in the region of the canine and incisor, and the maxilla in the region of the incisor. Sections were stained with H & E and a modified Picrosirius red procedure followed by microscopic examination under crossed polars. Fiber thickness was determined with the aid of a calibrated ocular micrometer using an oil immersion xlOO objective. Polarization colors were determined for thin (0.8 urn) and thick (1.6-2.4 jam) fibers. Polarization colors of thin fibers ranged from green to yellow and no differences were found between the experimental group and the controls. No differences between the groups were also noted when polarization colors of thick fibers from female dogs were recorded. However, in the male group, polarization colors of thick fibers ranged between blue-green to yellow in treated animals, while in controls, colors ranged between green-yellow to yellow-orange and orange. The green to yellow color of thick fibers in the hyperplastic gingiva of oxodipine treated beagle dogs (males) suggests that the collagen found in these lesions might be composed of procollagens, intermediates, or other pathologic collagens and not of normal, tightly packed fibers.

Scanning Tunneling Microscopy of Polyhedral Structures on M0S2 M. Hershfinkel, L.A. Gheber, V. Volterra and G. Gorodetsky
Department 0/Physics, Ben Gurion Univ., Beer Sheva, ISRAEL.
ABSTRACT During the growth process of M0S2 thin films it was observed that closed polyhedral structures were formed (R. Tenne et al.). M0S2 is a layered semiconductor with a planar hexagonal geometry. The closed structures that were observed may be an anlogy to the carbon fullerenes and nanotubes. We imaged the M0S2 films with a Scanning Tunneling Microscope (STM) in ambient conditions. We found that the samples had regions covered with polyhedral and round structures of different sizes. The smaller structures are usually tubes with dimensions as small as 30 x 60 Angs. The larger structures are more round and have typical diameters of 400 Angs. Their height is of the same order of magnitude as their other dimensions. Structures of similar dimensions are clustered together. The top of these structures is always round and no flat regions were found. In measurements of the angles of the polyhedra we found discrete values of 120> 240 and some sharp angles of -60, ~75> ~90. Our results are in agreement with the TEM measurements done by Margulis et al.

f :"V. V

Microscopic phase stability of the dilute magnetic semiconductor Cdi-xFexSe
E. Klein (1), M. Homyonfer (2), W. Giriat (3), R. Tenne (2)
1. Laboratory of Electronmicroscopy, Biological Services, Weizmann institute,
Rehovot 76100, Israel 2. Department of Materials and Interfaces, Weizmann Institute, Rehovot Israel 3. Centro de Fisica, Institutuo Venezolano de Invistigaciones Cientificas, Apartado 1827, Caracas 1010A, Venezuela 76100,
The solubility of Fe in CdSe crystal has been studied in detail in the past. Using powder diffraction and atomic absorption spectroscopy, it was concluded that the upper limit for the solubility of iron in CdSe is 12-15%, and the actual Fe concentration in a solid solution agrees with the nominal Fe concentration (calculated from the concentration of the reactants). In the present study this question is re-examined using techniques that probe the average properties of the solid solution, such as x-ray diffraction, and techniques that probe the local structure and composition of the solid solution on the micron scale, such as energy and wavelength x-ray dispersive microprobe analysis. It is shown that the solubility of Fe in the CdSe matrix is appreciably smaller than the nominal value, and that microsegregation is obtained at a much lower Fe concentration than previously concluded. The immiscibility of Fe in this matrix is consistent with the large deviation of the lattice constants from Vegard law. It is attributed to the large strain exerted on the lattice upon substitution of Cd by the much smaller Fe ion.

Electron Microscopy Sciences

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Agar Scientific Ltd.

66a Cambridge Rd. Stansted, Essex CM24BDA U.K. Tel: (0279) 813519. Telex 81667. Fax (0279) 815106

Crystalbond Adhesives

These adhesives are used as a temporary bond for holding delicate crystals, metallurgical samples, glass components and ceramic substrates for dicing, slicing, drilling, grinding and polishing. Crystalbond adheres readily to metals, glass and ceramics and can be washed away using various solvents after machining. We offer Crystalbond"" 509 and 555. 509 is recommended for precise high purity work since it leaves no residue after dissolving and does not clog the diamond wheels as can occur with conventional waxes. It offers excellent adhesion and is dissolved in acetone. 555 is used where it is necessary to have a temporary bond which is water soluble. B7297 Crystalbond 509, stick B7298 Crystalbond 555, approx. 150g

Silver Epoxy Kit

This is a two part electrically conductive silver epoxy suitable for mounting SEM samples, and for other applications such as repairing circuit boards for solderless electronic connections. G3349 Silver epoxy kit, 14g

Conductive Silver Pen

This pen is suitable for affixing samples to SEM stubs. It is designed for making instant conductive silver traces, the specially formulated silver-bearing thermoplastic polymer dries very quickly at room temperature. The valved pen tip allows a smooth flow with normal writing pressure and is spring loaded to prevent clogging. Each pen contains approximately 30 metres of trace. G3342 Conductive silver pen

JEOL Specimen Holder Kit

Designed to fit the 800 series JEOL scanning electron microscope, this kit enables a wide range of irregularly shaped samples to be examined without involving complicated mounting procedures. The sample holders fit in to the special sledge (supplied) which has a dove-tailed base to fit the JEOL airlock system. The kit contains four holders, two of which have interchangeable jaws so that various shapes and sizes can be accommodated. A further holder accepts thin strip type samples where the sample is held by spring clips, and the fourth holder accepts three 12.5mm diameter pin type (Cambridge) stubs. G3366 JEOL specimen holder kit

Kneten Kneading Malaxage

Heizen Heating Chauffage

Temperieren Tempering Conditkmnement therniique

Pumpen Pumping/Pompage

Analysieren Analysing Analyses
OestHlMfen Distilling Distillation
Messen, Steuem, Regain Measuring, Controlling, Regulating Mesure, commande, rtta
Janke & Kunkel GmbH & Co. KG IKA Labortechnik Ihr Facfthandler: Your specialised dealer:

INSIGHT

TIME. REAL COLOR.
Use the affordable MNHT laser scanning confocal microscope as you would an inverted fluorescence scope. Place your sample on the stage, and view it via the oculars in phase. DIC or confocal fluorescence. in Real Color and in Real Time. If you see a field of interest, photograph i t. simply and in Real Color using an optional automatic photomicrographic system with 35 mm or Polaroid back.

Confocal Microscope

Laser Scanning
-JfUse the affordable toOOHT laser scanning confocal microscope as a confocal image acquisition system for your existing videobased imaging computer. Interfacing is. Real I-asy. simply attach your camera to the INSIGHT'S standard C mount.
Use the affordable MMWr laser scanning confocal microscope as a complete confocal imaging and quantitating system by using the INSIGHT-IQ" computer system. Highly advanced hardware and software utilizing simple pull-down menus allow you to acquire and quantitate 2D confocal images in space and/or time, and generate 3D reconstructions and rotations. Real Time. Real Color. Real Easy. all yours with the UBOHT laser scanning confocal microscope. Contact Meridian today and select the /WWW model that's right for you. You'll be surprised at how affordable your choice will be.
Cos 7 cells stained with PI and NBD-Phallacidin.

Instruments, Inc.

2311) Science Parkway Okemos. Michigan -18804 Phone: 800-247-8084 517-34"-7200 Fax. 5l7-i4-5'ft7 Meridian Instruments Europe lndustrieparkWest 75 B-9100 St. Niklass BELGIUM Phone: 3-7801760 Fax: 3-7781727 Meridian Instruments Far F.ast K K The Sonehara Bldg 2nd Floor 2-2.K? Higashi Nihtmhashi Chuo-ku. Tokyo H\' IAPAN Phone: O.V.iNAWi! 5 t Fax 0.V5JW-
DISTRIBUTORS: Austria: Salus Braumapharm, O222-54-25-3. Benelux: Analis. H1-225-OH5. France: Cylolah. I-.W0H32? Oermanv dunn Lahortechnik. 02083-4.V30t>. Israel: Curmha. 02-322051. Italy: Kontron Instruments. 02-50721 Korea: Hwa Yuong Medical 8r Science Co. 02-SSo-225. Spain: Cultek. l-72-O333. Switzerland: BioCcll Consulting. 0ftl-7121ftlti. United Kingdom: Biotech Instrumenf. 05K.! 5u2'KK