All binocular tubes permit the precise, reproducible setting of the users specific interpupillary distance. Swinging the eyepieces while retaining the interpupillary distance permits the viewing height to be changed easily by up to 40 mm. Furthermore, the ergonomic tubes provide different viewing heights and angles. Thanks to eyepieces with folding eyecups, the removal of spectacles during microscopy has become unnecessary.

Microscope stage

Ergonomic tube
The vertically adjustable stage drive is positioned very close to the focusing drive to permit one-handed operation of the microscope stage and focusing drive.
Relaxed, fatigue-free and efficient microscopy
Motorization for Easy Operation
Microscopy is particularly efficient if recurring procedures can be performed simply, quickly and reproducibly. The Axioskop 2 mot plus offers the ideal requirements: coded and motorized microscope components, easily accessible function controls on the stand, and sophisticated operating software.

Coding

The positions of both the quintuple and sextuple nosepieces are coded. The same applies to the five reflector turret positions. Therefore, the operating software knows at all times which magnification and which contrasting technique is being used. The required illumination intensity can thus be activated automatically. All the electronic data of the microscope configuration is transferred to the control and application software. Exact documentation of the microscope settings is guaranteed at all times.
Motorized focus via traditional coarse/fine drive

Motorized condenser

Motorization
The Axioskop 2 mot plus features one constantly motorized component: the z-drive. Nevertheless, manual operation is also possible via a traditionally styled coarse / fine drive on both sides of the stand. Increments of 50 nm are ideal for a variety of applications. Of course, motor control with z-position readout is also possible via the software. The motorized reflector turret permits the change from reflected light to transmitted light and vice versa at the push of a button. The required light intensity is adjusted automatically. In addition to fluorescence filter sets, the reflector turret also accepts additional magnification optics Optovar modules for transmitted light techniques.

The motorized shutter in the reflected-light beam path prevents unnecessary bleaching of the fluorescence dyes. The possibility of integrating shutter functions in the software is particularly beneficial for time-lapse experiments. Further motorized components include the universal condenser, an external excitation filter wheel and the microscope stage. Integration of all the motorized components in the application software turns the microscope into a screening system.
Endothelial cells of the pulmonary artery, triple fluorescence Cystadenolymphoma, immunohistochemical staining of proliferating cells, brightfield

Memory Function

Thanks to the coded and motorized components, all microscope settings are known at all times. This permits the operating software to control all settings in accordance with default values which can be defined as required. For example, the required contrasting technique is automatically set after an objective change: brightfield, phase contrast or DIC, each with the correct condenser aperture and the required light intensity. Entire microscope configurations can be easily defined, applied and modified.
Fast change of microscope technique
Condenser aperture automatically matched to the objective aperture
Adenocarcinoma, immunocytochemical staining of tumor cells, brightfield
Tongue, hematoxylin and eosin staining, brightfield
Transmitted Light Microscopy
Traditional microscopy techniques such as brightfield, darkfield, phase contrast, differential interference contrast (DIC) and polarization can be performed without any restriction. The positioning of the DIC analyzer as a module in the reflector turret is particularly beneficial, since turning of the reflector turret permits the fast change from DIC to brightfield or phase contrast.

Brightfield

The traditional microscopy technique for stained histology specimens such as tissue sections and smears.

Darkfield

The contrasting technique for the display of minute structures at or even below the resolution limits of a light microscope.

Phase contrast

Ideal for fine tissue and cell structures in very thin, unstained or only slightly stained specimens.

Polarization

Motorized and coded components make this function extremely convenient.
The technique for the display of birefringence features of crystals and biological molecules.
Thin section of bone, brightfield, darkfield, phase contrast

Universal condenser

Arthritis crystal, polarization

DIC principle

Differential Interference Contrast (DIC)

Analyzer module

Nomarski interference contrast is ideal for thick, unstained specimens. This technique provides optical sections with optimum resolution, thus permitting the clear identification of fine structures in defined focal planes. The contrast is set via objective-specific DIC sliders in the nosepiece, so the contrast is always maintained once it has been set. The DIC prisms in the condenser are available with integrated polarizer. When reflector turret and condenser are motorized, a single push of the button is sufficient to change from epi-fluorescence to transmitted light DIC.

DIC slider

DIC prism

Polarizer

Cell culture, DIC
Illumination and Contrasting in Transmitted Light
The transmission parameters of the transmitted light beam path permit all contrasting techniques in the visible light range. The light intensity can be set either via modification of the lamp voltage or by maintaining the color temperature with neutral-density filters. The switchable filter mount also accepts color filters as required.

The condenser system

The condenser plays a major part in the setting of the various contrasting techniques and when properly adjusted ensures efficient and homogenous specimen illumination. All condenser systems can be set for Khler illumination.
Beam path Transmitted Light

Light source

The easily adjustable standard 100 Watt halogen light source provides sufficient light intensity for all the traditional transmitted light contrasting techniques.
Condensers for every technique
The achromatic-aplanatic universal condenser with aperture 0.9 is suitable for almost all applications. Whether manual or motorized, it covers the entire magnification range from 1x to 100x. Its seven positions provide ample space for all the contrasting components of traditional microscopy: phase stops 1 to 3, DIC prisms I to III with or without polarizer, darkfield stop and polarizer for transmitted-light polarization microscopy. All motorized condenser functions can be triggered at the push of a button or via the AxioVision application software. The achromatic switching condenser with aperture 0.9 is suitable for less complex brightfield and phase contrast applications.
Oil immersion objectives and condensers with oil immersion optics permit optimum resolution of the microscope image. The achromaticaplanatic condenser with aperture 1.4 has been optically corrected for this purpose. Whenever specimen vessels require long working distances, the achro-
matic condenser 0.8 with 7 mm working distance is used for brightfield, phase contrast and DIC applications. Complex darkfield with special darkfield condensers: oil immersion with the ultra condenser 1.2/1.4, dry applications with the dry darkfield condenser 0.8/0.95.

Epi-Fluorescence Microscopy
Fluorochromes and special labeling techniques are being used more frequently where the accurate reproduction of defined cell and tissue structures is required. Today, epi-fluorescence is the standard fluorescence technique. It is particularly efficient and permits a compact design of the filter modules. Furthermore, epi-fluorescence images and those provided by the transmitted light technique can be combined in a single image.
Today, traditional fluorescence dyes such as DAPI, FITC, Rhodamine and Texas Red are increasingly being replaced by even more efficient fluorochromes, e.g. Alexa Fluor and Cyanin. Examinations of living specimens in transmitted light are supplemented and extended with fluorescent proteins (CFP, GFP, etc.). Experiments previously only possible in-vitro can now be performed directly in the living cell. The Antiflex epi-polarization system visualizes the adhesion structures of cells on glass substrates. Together with fluorescence techniques, this permits the analysis of the function and structure of cytoskeletal elements.
Hep-2 epithelial cells, immunofluorescence of Cyclin I in cell nuclei (FITC)
Hep-2 epithelial cells, immunofluorescence of mitochondria (FITC)
Human endothelial cells, quadruple fluorescence DAPI, Alexa 350, Alexa 488, Phalloidin-Alexa 594. J. Zbaeren, Inselspital, Bern/Switzerland
Human endothelial cells, quadruple fluorescence DAPI, Alexa 350, Alexa 488, Phalloidin-TRITC. J. Zbaeren, Inselspital, Bern/Switzerland
Tongue, rat, double fluorescence Alexa 594, DAPI. J. Zbaeren, Inselspital, Bern/Switzerland
13 C. elegans, GFP-marked neurons. H. Hutter, MPI Heidelberg/Germany
Illumination and Contrast in Fluorescence

Adjusting aid

The beam path required for epifluorescence can be integrated and retrofitted into the Axioskop 2 plus and Axioskop 2 mot plus microscopes. The viewing height remains unchanged, since the stand has been prepared for this integra-

Reflector turret

The 5-position reflector turret is always included in the basic stand. Manual, coded and motorized versions are available. It permits easy switching between transmitted and reflected light techniques with very convenient push-button operation in the motorized version.

P&C modules

Fluorescence configurations
The filters required for fluorescence microscopy are integrated into the Push&Click modules which can be attached to the reflector turret and removed from it without any tools. Therefore, many different fluorochromes can be examined quickly and efficiently. The patented Light Trap principle provides optimum fluorescence signals without any disturbing background noise.

External filter wheel

The beam path is equipped with an adjustable and centerable luminousfield diaphragm and aperture stop. The adjusting aid is available for easy adjustment of the illumination source after a lamp change.
A further increase in flexibility of fluorescence microscopy is provided by the motorized 8-position filter wheel (option) for the Axioskop 2 mot plus. Multiple fluorescence applications are easy to perform.
Filter wheel Change of filter cubes the Push&Click technique The 5-position reflector turret for Push&Click filter cubes

Beam path Fluorescence

The high-energy HBO 103 mercury vapor short-arc lamp is used for almost all fluorescence techniques. Clearly allocated and easy-to-operate setting screws in the lamp housing permit fast and precise lamp adjustment which can be monitored through the window of the adjusting aid. The lamp is inserted into the precentered clamping device without any need for a special tool.

Light trap

Stray radiation passes through a beam splitter (2), leaves the filter cube (1) directly through the rear opening (3) and is then directed out of the beam path through the conical mount (4) of the filter turret (5). The results are self-explanatory: considerable contrast enhancement, increased image definition and brilliance, higher sensitivity due to an improved signal-to-noise ratio.
Beam path in a traditional reflector module Beam path with light trap

A-Plan objectives

Objectives for Observation and Measurement
Infinity optics were designed and computed by August Khler as far back as the thirties. But it was not before the invention of ICS (Infinity Color-corrected System) in 1986 that this optical system became the centerpiece Microscopy. Simply said, the function of the ICS optics is to achieve optimum performance by using the minimum number of optical elements. Since every optical element contributes to the reduction of light transmission, the minimization of optical components means the maximizaFluar Plan-Neofluar objectives Achroplan objectives

modern

tion of the overall optical performance. All in all, this resulted in visible improvements of image contrast, brightness and detail resolution.

objectives

Plan-Apochromat objectives

An attractive alternative
Are you looking for a budget-priced alternative to Achroplan objectives? Here it is: A-Plan objectives are reliable in daily routine, clinical diagnostics and research. They provide rich contrast, are suitable for fluorescence and can be used with eyepieces with a field of view of up to 23 mm. They are applicable for brightfield and phase contrast techniques.

A sound basis

These are the basic objectives for your daily transmitted light routine and for epi-fluorescence microscopy with visible-light excitation. Thanks to their high image flatness across field diameters of 23 mm, Achroplan objectives are ideal for image documentation (photomicrography and digital microscopy). LD-Achroplan objectives are also available for extremely long working distances with high apertures. Special Achroplan water objectives are provided for applications in physiology.

The allrounders

Where flexibility and a wide range of techniques are prime concerns, the universal semi-apochromatic Plan-Neofluar objectives are the best answer. With a transmission range up to the near UV, achromatic correction, optimum working distances, low strain and high numerical apertures, they are ideal for brightfield, darkfield, phase contrast, DIC, polarization and fluorescence. High contrast and clear definition make them the right choice for image processing and analysis.

The photon gatherers

The Fluar line is designed for maximum transmission and photon gathering efficiency. Made of special optical glass types, these objectives feature high numerical apertures, high contrast and high transmission for the entire visible spectrum up to the near UV. If you want to detect the faintest fluorescence, Fluar objectives from Carl Zeiss are your best choice.
The ultimate imaging experts
Combining the highest color correction with highest numerical apertures, Plan-Apochromat objectives deliver the ultimate in resolution and image definition and thus provide the finest details and subtlest color nuances. The large apertures enable brilliant brightfield and DIC images and ensure high performance in fluorescence.

Microscope Stages to Move the Specimen
Vertically adjustable control elements on the stage drive
Three stage models are available for the precise movement of the specimens in the x-y direction: manual, coded and completely motorized. They are attached to the stage and condenser carriers of the Axioskop 2 plus and Axioskop 2 mot plus microscopes without any need for special tools.

Manual version

Mechanical stages, manually moved in x and y, with a travel range of 75x50 mm, are the most traditional types of microscope stages. Depending on requirements, they are available with coaxial x-y drive on the right or left. The centerable rotary mechanical stage 75x50/240R with x-y drive on the right provides a rotation range of 240. The controls can be positioned at any height required and their smoothness set as required by the user.

Coded and manual version

An electrical vernier scale permits the reading of x-y data of the mechanical stage 75x50R. The x-y positions can be displayed either directly or transferred to an application software. The mechanical stage 75x50 mot features its own control unit. The specimen can be moved optionally via a 2-axis joystick or a coaxial electrical drive.

Fully motorized control

The scanning stage DC 4 x 4 has been designed for entirely motorized experiments. The connection to the control computer is made via a motor control unit. However, the x-y movements of the stage can also be performed via an optional control panel for two axes.
Mechanical stage with stage drive on the right Mechanical stage with stage drive on the left

Specimen holders

Motorized mechanical stage
A wide variety of specimen holders is available for microscopy. From the simple model for manual mechanical stages to universal heating frames for complex experiments and various inserts for fully motorized microscope stages.

DC scanning stage

A Single System for Documentation and Evaluation
Image documentation and analysis are a must in daily routine and research. No matter whether you use an SLR, video or digital camera, the binocular phototubes permit easy recording and display of the specimens.

Digital photography

Traditional, time-consuming photography is being replaced more and more by cameras with light-sensitive sensors. The image of the specimen is readily available on the monitor and can be processed immediately. Documentation is further facilitated by the modern, digital AxioCam microscope camera, featuring easy operation and optimum resolution.
Digital image documentation

Amo International Inc.

Central Quality Control Laboratory

QC - Image Archive

Documentation is supported by the application-specific AxioVision software. Image recording, simple image processing, annotations and image archiving can be performed in no time at all. The hand-written, time-consuming recording of test procedures is a thing of the past. And if required, the results can be printed quickly on customized report forms.

Keywords: Comments:

Pol color etched Material is color-etched FeC/Ni Delivered to MNO Ltd Delivery Date: Aug. 31, 1998 State: Final approval required

MNO Institut

ZL GmbH
Musterstrae 4 D-80801 Mnchen Telefon: +49 (89) 95 97-0 Fax: +49 (89) 9597-100

Bild Datum Kommentar

Osteo00012 27.07.98 Knochenschnitt mit Goldner-Frbung (rot: Osteoid, grn: Trabekel), Wachstumsaktivitt Bildaufnahme mit Axioskop, Durchlicht, PlanNeofluar 20 x, SONY 950 3-CCD RGB Kamera. Osteoid Trabekel

Stichwort

AG Zellmorphologie
Titel Datum Autor Kommentar
D-10000 Musterstadt Zahn00014 Telefon: +49 (99) 9597-0 27.07.98 M. Meier Grenzflche zwischen Zahnimplantat (schwarz) und umgebendes Gewebe (rot: Knochen, wei: Bindegewebe) knstliche Zahnwurzel aus Titan Adaptation an den Knochen. Bildaufnahme mit Axioskop. Durchlicht, PlanNeofluar 5x, SONY MC 3309 RGB Kamera Prof. Dr. Dr. O. Meier Gebude 08 / 15

Zahnimplantat

Fluor00002 22.05.98 Dr. Dr. Mller Mehrfach-Fluorenszenz: DAPI FITC Texas Red Fluorescence Multichannel
AxioVision, Ideal for Documentation
Together with the AxioCam digital camera, the AxioVision application software and its additional modules are ideal for all image-related documentation tasks using the Axioskop 2 plus and Axioskop 2 mot plus microscopes. Image acquisition, processing and archiving are time-saving and low-cost procedures. All the motorized microscope components are integrated into the application workflow and can be controlled via the software. All the required documentation parameters such as magnification factor, contrasting technique, exposure times, etc., are ready and available at the push of a button. Time-consuming test records are a thing of the past.
Easy insertion of text and graphics elements Triple fluorescence

Digital Photography

AxioVision system package
Even the basic version of AxioVision permits complete control of all motorized microscope components, in addition to image documentation and archiving. Suitable software modules are available for complex applications such as multichannel fluorescence, time-lapse microscopy and z-stacks, or combinations of these applications. This permits complex experiments to be easily configured and triggered at the push of a button. All system parameters are recorded automatically and are available at all times for recurring experiments, thus ensuring the reproducibility of recording conditions.

MC U 28

POW ER ERR OR RUN 7
Stage carrier with condenser carrier 452327-0000-000 or Condenser carrier for FS 000000-1022-999
Axioskop 2 FS plus microscope stand 000000-1066-600 Axioskop 2 FS MOT microscope stand 000000-1066-601
Filter set VIS/IR 000000-1031-489

Video adapter C-mount

Video adapter ENG-mount
Video zoom 44 C 1/3" (3 CCD) 0.33x. 1.6x 452989-0000-000 Video adapter 60 C 1/3" 0.4x 456108-0000-000 Video adapter Video adapter Video adapter 60 C 1/2" 0.5x 60 C 2/3" 0.63x 60 C 2/3" 1.0x 456106-0000-000 000000-1069-414 456105-0000-000 (000000-1069-415 in preparation)
Video zoom 44 ENG 1/2" 0.5x. 2.4x 452984-0000-000
Video adapter 44 ENG 1/2" 0.63x 452992-0000-000

Video camera as desired

Video adapter 60 ENG 2/3" 1.0x 456115-0000-000 Adapter from 60 to 44 456140-0000-000
Binocular phototube 30/23 (70:30) 452344-0000-000
Binocular ergonomy phototube 6-25/23 (100:100) 452342-0000-000
Binocular tube 30/23 452340-0000-000
Binocular ergonomy phototube 20/23, height adjustable 000000-1104-296
Binocular ergonomy tube 20/23, height adjustable 000000-1104-293
Double video adapter 000000-1058-640
Binocular phototube with sliding prism 30/25 (100:0/50:50/0:100) 452143-0000-000
Binocular phototube with two TV ports 30/25 (100:0/50:50/0:100) 452145-0000-000
Double TV-tube mot. with 2 outputs 000000-1054-146 Accessories acc. price list
Projector tube for image interpolation 452181-0000-000 Optovar intermediate tube 1.0x/1.25x/1.6x/2.0x/2.5x cod. 452175-0000-000 Intermediate zoom tube 1.0x. 2.5x cod. 452180-0000-000

Adapter 452969-0000-000

Polarizer D, fixed 453615-0000-000
Polarizer D, rotatable 453620-0000-000
Polarizer fixed with lambda plate, rotatable 445226-0000-000
Senarmont polarizer DIC 453622-0000-000
Color filter carrier 3x for filter d=32 mm 452159-0000-000 Polarizer with filter holder 000000-1118-003
Cable release 416167-0000-000 Single-lens reflex camera housing CONTAX-ARIA 000000-1057-127
Compact digital camera, e.g. SONY DSC-SMB, d=52mm (D) 000000-0425-186
T2-adapter for CONTAX 416010-0000-000 (additional T2-adapter acc. price list)

Camera adapter D40 M52x0.75 000000-1096-522
Digital camera adapter 44 M52x0.75 000000-1108-984 Microscope camera MC 80 See price list for equipment and accessories
Adapter T2 2.5x for SLR 456005-0000-000
Connector 60 for microscope camera d=30 456006-0000-000
Attachment for 60-44 456140-0000-000

Leve 1 l Leve 2 l Hour s

Carl Zeis
Active coobservation device for the Axioskop 2 (transmitted light) with light point and power unit 452379-0000-000 for tubes 452340-0000-000 (only rear); 452342-0000-000 (in front and rear)
Variable intensity lamp control AttoArc 2 N HBO adjustable 100-120-220-240 V 50/60 Hz 000000-1007-975
Incident light equipment 000000-1150-310
8-position filter wheel for dia. 25 mm excitation filters (only with MOT stand, PC and microscope control software) 000000-1008-106

HOU RS 230

POW ER

CUR TIS

SAF ETY
Transformer HBO 103 ebq 100 dc 000000-1003-928
Adjusting aid for HBO/XBO 452369-0000-000
Lamp housing N HBO 103 000000-1007-980 Achromatic collector 000000-1007-978 Collector N HBO 103/XBO 75 000000-1007-976 or Quartz collector N HBO 103/XBO 75 000000-1007-977 Mercury vapor short-arc lamp HBO 103 W/2 380301-9350-000
6-position FL filter slider 446377-0000-000
Transformer mbq 52 ac-z for HBO 50 220-240 V 000000-1113-833 Lamp housing HBO 50 incl. lamp socket 447220-0000-000 Collektor HBO 50/SF 25 447270-0000-000 Super-pressure mercury lamp HBO 50 381619-0000-000
Axioskop 2 plus microscope stand 000000-1116-576 6-position nosepiece H W 0.8 with 5-position reflector turret 000000-1059-202 5-position nosepiece H DIC W 0.8 with 5-position reflector turret 000000-1056-430
Axioskop 2 mot plus microscope stand 000000-1130-660 5-position nosepiece H DIC W 0.8 cod. with coded, 5-position reflector turret 000000-1058-255 6-position nosepiece H W 0.8 cod. with coded, 5-position reflector turret 000000-1059-203 5-position nosepiece H DIC W 0.8 cod. with motorized, 5-position reflector turret 000000-1144-840 Shutter for incident light equipment 000000-1144-850 (only in combination with nosepiece 000000-1144-840)
Filter set D 000000-1058-230
Notebook 000000-1056-852/853 Microscope control software 000000-1068-HAL lamp housing with collector, lamp mount 447219-0000-000 Halogen lamp 12 V 100W 380079-9540-000
for external power supply HAL absoluteley essential for Axioskop 2 FS
Analyzer module for transmitted light 000000-1050-958 Optovar module 1.25x 000000-1046-284 Optovar module 1.6x 000000-1046-283 Optovar module 2.5x 000000-1046-282

Analyzer DIC/FL 452374-0000-000 (additional to barrier filter on FL or optovar module)
Power supply 12 V DC 100 W stabilized 000000-1128-574
Reflector module FL 000000-1046-281 Reflector module H 000000-1046-274 Filter sets according price list
Achr.-aplanatic universal condenser 0.9 H MOT 000000-1159-528 Achr.-aplanatic universal condenser 0.9 H D Ph MOT 000000-1159-529 Achr.-aplanatic universal condenser 0.9 H D Ph DIC MOT 000000-1159-531 Polarizer for condenser (000000-1159-531) 000000-1028-973 DIC slider according price list
Compensator lambda, 6x20 473704-0000-000 Compensator lambda/4, 6x20 473714-0000-000 Compensator lambda, rotary, +/-8 6x20 473710-0000-000 Wedge compensator 0-4 lambda, 6x20 000000-1140-663
Antiglare screen 452163-0000-000
DIC prism I/0.9 (with polarizer) 000000-1005-002 DIC prism II/0.9 (with polarizer) 000000-1005-003 DIC prism III/0.9 (with polarizer) 000000-1005-004 DIC prism I/0.9 000000-1004-901 DIC prism II/0.9 000000-1004-902 DIC prism III/0.9 000000-1004-903
Adapter ring W 0.8 without NG 000000-1104-291
Adapter ring W 0.8 NG 0.25 000000-1104-289 Adapter ring W 0.8 NG 0.10 000000-1104-290
Specimen marker 000000-1105-072 Refill set for specimen marker 000000-0428-327
Objectives (ICS objectives according to price list)
Achr.-aplanatic universal condenser 0.9 H 000000-1159-526 Achr.-aplanatic universal condenser 0.9 H D Ph 000000-1159-527 Achr.-aplanatic universal condenser 0.9 H D Ph DIC 000000-1159-530 Polarizer for condenser (000000-1159-530) 000000-1028-973 Achromatic switching condenser 0.9 H 000000-1017-688 Achromatic switching condenser 0.9 H D Ph 000000-1017-690 Ultra condenser 1.2/1.4(0.75-1.0) 465500-0000-000 Dry darkfield condenser 0.8/0.95(0.6-0.75) 465505-0000-000
DIC prism II/1.4 445375-0000-000 DIC prism III/1.4 445376-0000-000 Achr. condenser 0.8 H d=6.7 mm 000000-1087-444 DIC prism III /0.8 000000-1087-445
DIC prism III/0.8 445485-0000-000
Achr. aplanatic condenser 1.4 H Ph DIC d=0,5 mm 445453-0000-000
Achr. condenser 0.8 H D Ph DIC d=6.7 mm 445445-9901-000

Darkfield illuminator 445314-9901-000
Condenser holder Z for darkfield condenser 445323-0000-000

488 438

260 333
For further details, please contact:
Carl Zeiss Light Microscopy P.O.B. 37030 Gttingen GERMANY Phone: ++Telefax: ++E-Mail: micro@zeiss.de www.zeiss.de/micro
Design and specifications are subject to change in the interest of technical development

40-010 e/03.02

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Axioskop 2 plus Axioskop 2 mot plus
START-UP Initial start-up

Carl Zeiss

2.1.10 (1)
HAL 100 halogen illuminator Attachment illuminator of HAL 100 halogen
Remove cover from transmitted-light tube (2-11/3). Insert dovetail 2-12/2) of lamp housing (2-11/1) in transmitted-light tube (2-11/3) on the instrument rear and use the SW 3 ball-headed screwdriver to tighten clamping screw (2-11/2). Connect 3-pin lamp plug (2-11/5) to 3-pin 12 V/100 W socket (2-11/4) on the instrument rear.

(2) a)

Adjustment illuminator

HAL 100

halogen

Fig. 2-11

Attachment of HAL 100 halogen illuminator

Coarse adjustment

Loosen clamping screw (2-11/2) and remove the halogen illuminator from the microscope stand. Switch on microscope as described under 0. Direct the light beam against a projection area (wall) approx. 3 m away.
Do not look into the light exit opening of the illuminator. Set adjusting screw (2-12/3) using SW 3 ballheaded screwdriver in such a way that both images of the lamp coil are as sharply focused as possible on the projection screen. Then set adjusting screws (2-12/4 and 5) in such a way that the lamp coil of one image exactly fills the gaps in the reflector image (2-12/1).

Fig. 2-12

Adjustment of HAL 100 halogen illuminator

B 40-075 e

START-UP Attachments and conversions
Changing the HAL 100 halogen lamp
Switch off the microscope as described in section 2.1.9, remove plug (2-11/5) from 12 V / 100 W socket (2-11/4) and allow the instrument to cool down for approx. 15 mins. Loosen screw (2-29/5) until the lamp housing can be removed in an upward direction. Press both spring levers (2-29/3) downwards and pull out the old halogen lamp (2-29/2) in an upward direction. Press both spring levers downwards, insert new lamp, with protection cap (2-29/1) being attached in lamp base (2-29/4), let go the spring lever and pull the protection cap off. Press spring lever briefly downwards again to center the lamp. Attach lamp housing again clamping screw (2-29/5). and tighten
Fig. 2-29 Changing the halogen lamp
Changing the HBO 103 W/2 mercury pressure short-arc lamp
Before opening the lamp housing, make sure that no connection to the electrical line is available. The HBO 203 W/2 lamps may only be changed after they have cooled down. Allow the HBO 103 microscope illuminator to cool down for approx. 15 mins to avoid the risk of burns. The HBO 103 W/2 lamp may only be removed from the packaging and inserted in the HBO 103 illuminator if a protective mask and safety gloves are worn. All electrical clamping connections must be made carefully. Pronounced heat during operation may result in loose contacts.